Microbial Pathogenesis, Journal Year: 2022, Volume and Issue: 164, P. 105414 - 105414
Published: Jan. 29, 2022
Language: Английский
BioScience Trends, Journal Year: 2022, Volume and Issue: 16(4), P. 267 - 281
Published: Aug. 6, 2022
Recently, the emerging role of circular RNAs (circRNAs) in tumor development and progression has been a topic great interest. Nevertheless, effects hepatic stellate cell (HSC)-derived exosomes hepatocellular carcinoma (HCC) remain unclear. Here, we aim to explore potential effect HSC exosome-derived circWDR25 on aggressiveness HCC. Firstly, microarray analysis circRNAs was performed profile identify differentially expressed derived from activated by HCC cells. Subsequently, roles growth were confirmed through vitro vivo functional experiments. Moreover, RNA pull-down, dual-luciferase reporter assays, fluorescent situ hybridization (FISH) determine interactions circWDR25-miR-4474-3p-ALOX15 loop. Immunohistochemical also tissues peritumoral tissues. We found that overexpressed associated with survival recurrence patients promoted cells both vivo. Mechanistically, exogenous exosomal-derived regulated expression ALOX15 sponging miR-4474-3p ultimately inducing an epithelial-to-mesenchymal transition (EMT) CTLA-4 HSCs PD-L1 In conclusion, facilitated proliferation invasion via circWDR25/miR-4474-3p/ALOX15 EMT axes it cells, thus providing insights into mechanism mediated HSC-derived exosomal circWDR25.
Language: Английский
Citations
28
Bioengineered, Journal Year: 2022, Volume and Issue: 13(4), P. 9662 - 9673
Published: April 1, 2022
Circular RNAs (circRNAs) are crucial non-coding in the process of tumorigenesis. Nevertheless, biological function circ_0004277 acute myeloid leukemia (AML) is blurred. Microarray data circRNAs were utilized to evaluate circRNAs' differential expression AML. Quantitative real-time polymerase chain reaction (qRT-PCR) was executed determine and microRNA-134-5p (miR-134-5p) levels. The growth, migration invasion AML cells tested by cell counting kit-8 Transwell experiment. Dual-luciferase reporter gene experiment, RNA immunoprecipitation (RIP) experiment pull-down targeting relationship between miR-134-5p. Western blot assay used detect single stranded DNA binding protein 2 (SSBP2) expression. We observed that down-regulated AML, while miR-134-5p up-regulated. Functionally, overexpression or inhibition remarkably suppressed viability, invasion. Furthermore, served as a direct downstream target SSBP2 identified Compensation experiments showed mimics abolished on malignant phenotypes cells. Collectively, impedes development adsorbing up-regulating SSBP2.
Language: Английский
Citations
26
Critical Reviews in Oncology/Hematology, Journal Year: 2024, Volume and Issue: 196, P. 104312 - 104312
Published: Feb. 28, 2024
Language: Английский
Citations
6
Frontiers in Cell and Developmental Biology, Journal Year: 2021, Volume and Issue: 9
Published: Dec. 8, 2021
Metastasis is an important feature of malignant tumors, and the primary cause poor prognosis treatment failure, in addition to representing a potentially fatal challenge for cancer patients. Exosomes are small extracellular vesicles 30-150 nm diameter that transmit cargo, such as DNA, RNA, proteins, means intercellular communication. play crucial roles range human diseases, especially tumors. A growing number studies have verified circRNAs can be enveloped exosomes transferred from secretory cells recipient cells, thereby regulating tumor progression, metastasis. Exosomal regulate cell metastasis not only by signaling pathways, but also affecting microenvironment. Moreover, exosomal potential serve valuable diagnostic biomarkers novel therapeutic targets In this review, we summarize mechanism which modulate metastatic phenomena various types put forward prospects clinical applications therapy.
Language: Английский
Citations
30
Journal of Hepatocellular Carcinoma, Journal Year: 2022, Volume and Issue: Volume 9, P. 1029 - 1040
Published: Sept. 1, 2022
Abstract: Hepatocellular carcinoma (HCC) is currently the second leading cause of cancer-related deaths worldwide, with high morbidity and mortality. The clinical diagnosis HCC mainly depends on imaging technology, such as ultrasound computed tomography, serum biomarkers, alpha-fetoprotein (AFP). However, still hard to diagnose at an early stage due low sensitivity above mentioned traditional methods. Typically, diagnosed advanced when limited treatment options are available. It urgent identify effective biomarkers for HCC. Increasing evidence uncovered ncRNAs, including microRNAs (miRNAs), long non-coding RNAs (lncRNAs), circular (circRNAs), could be used in diagnosis. aim this review summarize our understanding circulating miRNAs, lncRNAs circRNAs fluid-based non-invasive aiming providing new insights into Keywords: hepatocellular carcinoma, microRNAs, RNAs,
Language: Английский
Citations
20
Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13
Published: Oct. 10, 2022
Hepatocellular carcinoma (HCC) is a complex and heterogeneous malignancy with high incidence poor prognosis. In addition, owing to the lack of diagnostic prognostic markers, current multimodal treatment options fail achieve satisfactory outcomes. Tumor immune microenvironment (TIME), angiogenesis, epithelial-mesenchymal transition (EMT), invasion, metastasis, metabolism, drug resistance are important factors influencing tumor development therapy. The intercellular communication these processes mediated by variety bioactive molecules regulate pathophysiological in recipient cells. Among molecules, non-coding RNAs (ncRNAs), including microRNAs (miRNAs), long (lncRNAs), circular (circRNAs), account for large part human transcriptome, their dysregulation affects progression HCC. purpose this review evaluate potential regulatory mechanisms ncRNAs HCC, summarize novel biomarkers from somatic fluids (plasma/serum/urine), explore some small-molecule modulators as drugs. Thus, through review, we aim contribute deeper understanding mechanisms, early diagnosis, prognosis, precise
Language: Английский
Citations
20
Biomedicine & Pharmacotherapy, Journal Year: 2022, Volume and Issue: 157, P. 114053 - 114053
Published: Nov. 30, 2022
Despite the high prevalence of gastrointestinal tumors, early diagnosis and treatment these tumors is limited by lack effective specific biomarkers therapeutic targets. Exosomes carry active molecules to mediate cell-to-cell communication, especially in tumor microenvironment, are promising targets for cancer. Circular RNAs (circRNAs) stably enriched exosomes show a unique circular structure, stability, conservation, tissue specificity. Exosomal circRNAs play important roles regulating cell proliferation, metastasis, angiogenesis, metabolism, immune microenvironment exhibit great potential as anti-tumor or tools. This review briefly introduces characteristics functions exosomes, systematically describes biological mechanisms exosomal tumors. article also summarizes detection methodology discusses their clinical significance
Language: Английский
Citations
20
World Journal of Gastroenterology, Journal Year: 2022, Volume and Issue: 28(46), P. 6433 - 6477
Published: Dec. 6, 2022
Hepatectomy is currently considered the most effective option for treating patients with early and intermediate hepatocellular carcinoma (HCC). Unfortunately, postoperative prognosis of HCC remains unsatisfactory, predominantly because high metastasis recurrence rates. Therefore, research on molecular mechanisms will help develop intervention measures to prevent or delay improve long-term survival patients. Herein, we review latest progress underlying lay a foundation improving understanding developing more precise prevention strategies.
Language: Английский
Citations
20
Environmental Science and Pollution Research, Journal Year: 2023, Volume and Issue: 30(24), P. 65237 - 65249
Published: April 20, 2023
Abstract The eukaryotic translation initiation factor 4A (eIF4A) family determines transcription efficiency by directly binding to precursor RNAs. One member, EIF4A3, modulates the expression of circRNAs. Circular RNA SCAP (circSCAP), a newly found circRNA, has been implicated in atherosclerosis. Yet, how circSCAP regulates cancer development and progression remains understudied. Here, we investigated function molecular mechanism tumorigenesis non-small-cell lung (NSCLC). CircSCAP was upregulated both NSCLC tissues cell lines mainly located cytoplasm. promoted which associated with poor prognosis patients NSCLC. sponged miR-7 upregulate small mothers against decapentaplegic 2 (SMAD2). knockdown undermined proliferation, migration, invasion abilities (SPCA1 A549), rescued either inhibiting or overexpressing SMAD2. Moreover, E-cadherin, while downregulating N-cadherin, Vimentin, MMP9 SPCA1 A549 cells, were abolished Additionally, markedly downregulated, whereas SMAD2 significantly tissues. MiR-7 inversely correlated In conclusion, this study demonstrates that is elucidates facilitates sponging upregulating provides novel target for early diagnosis treatment
Language: Английский
Citations
13
Cells, Journal Year: 2023, Volume and Issue: 12(16), P. 2036 - 2036
Published: Aug. 10, 2023
Hepatocellular carcinoma (HCC) is a primary liver cancer with high mortality rate and limited treatment options. Recent research has brought attention to the significant importance of intercellular communication in progression HCC, wherein exosomes have been identified as critical agents facilitating cell-to-cell signaling. In this article, we investigate impact macrophages both sources targets shedding light on intricate interplay between exosome-mediated macrophage involvement HCC pathogenesis. It investigates how derived from cells other cell types within tumor microenvironment (TME) can influence behavior, polarization, recruitment. Furthermore, section explores reciprocal interactions macrophage-derived cells, stromal immune elucidating their role growth, angiogenesis, metastasis, evasion. The findings presented here contribute better understanding offer new avenues for targeted interventions improved patient outcomes.
Language: Английский
Citations
13