Materials Today Nano,
Journal Year:
2024,
Volume and Issue:
26, P. 100483 - 100483
Published: May 21, 2024
Intracerebral
hemorrhage
(ICH)
is
a
common
cerebrovascular
disorder.
There
still
high
risk
of
severe
neurological
deficits
even
when
patients
survive
ICH.
The
application
new
treatment
techniques
can
reduce
mortality,
but
reversing
damage
caused
by
ICH
remains
challenging.
Although
cell-based
therapies
have
shown
promise
in
treating
cerebral
hemorrhage,
recurring
challenges
are
premature
apoptosis
transplanted
cells
accompanied
insufficient
numbers
and
lack
engraftment
the
target
tissue,
thus
limiting
therapeutic
efficacy.
Here,
we
show
that
hypoxic
preconditioning
combined
with
three-dimensional
(3D)
dynamically
cultured
gingival
lamina
propria-derived
MSCs
(GMSCs)
significantly
improves
cell
viability,
anti-apoptosis
ability,
efficacy
compared
traditional
monolayers.
Hypoxia-preconditioned
3D-GMSCs
also
exhibit
notable
neuroprotective
promote
extracellular
vesicles
(EVs)
biogenesis
potential
as
evidenced
transwell
vitro
intervention
experiments
RNA-seq;
further
confirmed
characterization
analysis
EVs.
Furthermore,
3D-GMSCs-EVs
derived
from
not
only
had
better
tissue
uptake
capabilities
reduced
bleeding
mice
improved
motor
function.
Subsequent
on
found
hypoxia-preconditioned
promoted
reduction
neuronal
oxidative
stress
levels
mitochondrial
function
recovery.
This
study
innovated
culture
method
GMSCs,
demonstrated
effective
modification
EVs,
provided
ideas
for
establishing
cell-free
hemorrhage.
Frontiers in Pharmacology,
Journal Year:
2023,
Volume and Issue:
14
Published: March 22, 2023
Ischemic
stroke
has
been
a
prominent
focus
of
scientific
investigation
owing
to
its
high
prevalence,
complex
pathogenesis,
and
difficulties
in
treatment.
Mitochondria
play
an
important
role
cellular
energy
homeostasis
are
involved
neuronal
death
following
ischemic
stroke.
Hence,
maintaining
mitochondrial
function
is
critical
for
survival
neurological
improvement
stroke,
mitochondria
key
therapeutic
targets
cerebral
research.
With
the
benefits
efficacy,
low
cost,
safety,
traditional
Chinese
medicine
(TCM)
great
advantages
preventing
treating
Accumulating
studies
have
explored
effect
TCM
from
perspective
regulating
structure
function.
In
this
review,
we
discuss
molecular
mechanisms
by
which
Furthermore,
summarized
current
advances
modulating
mitochondria.
We
aimed
provide
new
enlightenment
prevention
treatment
Oxidative Medicine and Cellular Longevity,
Journal Year:
2021,
Volume and Issue:
2021(1)
Published: Jan. 1, 2021
The
mechanism
of
Golgi
apparatus
(GA)
stress
responses
mediated
by
GOLPH3
has
been
widely
studied
in
ischemic
stroke,
and
the
neuroprotection
effect
olfactory
mucosa
mesenchymal
stem
cells
(OM-MSCs)
against
cerebral
ischemia/reperfusion
injury
(IRI)
preliminarily
presented.
However,
exact
role
OM-MSCs
GA
response
following
IRI
remains
to
be
elucidated.
In
present
study,
we
used
an
oxygen-glucose
deprivation/reoxygenation
(OGD/R)
model
reversible
middle
artery
occlusion
(MCAO)
simulate
vitro
vivo.
Our
results
showed
that
level
protein,
reactive
oxygen
species
(ROS),
Ca2+
was
upregulated,
SPCA1
downregulated,
fragmentation
increased
stroke
models,
OM-MSC
treatment
clearly
ameliorated
these
Subsequently,
knockdown
PEDF
using
PEDF-specific
siRNA
further
demonstrated
secretion
protected
OGD/R-treated
N2a
MCAO
rats
from
response.
Additionally,
rescue
experiment
specific
pathway
inhibitors
suggested
could
promote
phosphorylation
PI3K/Akt/mTOR
pathway,
thereby
mitigating
OGD/R-induced
excessive
autophagy.
conclusion,
minimized
IRI,
at
least
partially,
through
PEDF-PI3K/Akt/mTOR
pathway.
Cell Biology International,
Journal Year:
2022,
Volume and Issue:
46(9), P. 1309 - 1319
Published: July 13, 2022
Abstract
The
Golgi
apparatus
is
a
membrane‐bound
organelle
that
functions
as
central
role
in
the
secretory
pathway.
Since
discovery
of
apparatus,
its
structure
and
function
have
attracted
ever‐increasing
attention
from
researchers.
Recently,
it
has
been
demonstrated
metal
ions
are
necessary
for
to
maintain
proper
functions.
Given
play
an
important
various
biological
processes,
their
abnormal
homeostasis
related
many
diseases.
Therefore,
this
paper,
we
reviewed
uptake
release
mechanisms
Ca
2+
,
Cu,
Zn
.
Furthermore,
describe
diseases
associated
with
imbalance.
Heliyon,
Journal Year:
2023,
Volume and Issue:
9(7), P. e17874 - e17874
Published: July 1, 2023
Oxidative
stress
is
essential
in
brain
injury
after
intracerebral
hemorrhage
(ICH).
Ferroptosis,
iron-dependent
oxidative
cell
death,
overwhelms
the
antioxidant
system.
Recently,
Olfactory
mucosa-derived
mesenchymal
stem
cells
(OM-MSCs)
hold
great
potential
for
treating
ferroptosis-mediated
damage
ICH.
However,
massive
grafted
possibly
caused
by
a
hostile
host
microenvironment,
lessens
effectiveness
of
OM-MSCs.
Therefore,
it
necessary
to
develop
strategies
upregulate
therapeutic
efficacy
OM-MSCs
Curcumin,
well-established
traditional
herbal
substance,
has
potent
property.
In
present
study,
curcumin
preconditioning
might
enhance
anti-oxidative
activity
OM-MSCs,
thereby
augmenting
vitro
model
ICH,
we
demonstrated
that
curcumin-preconditioned
co-culture
more
effective
attenuating
injury,
stress,
and
ferroptosis
neuronal
compared
native
treatment.
vivo
transplantation
also
showed
better
neuroprotective
effects.
Moreover,
pretreatment
promoted
survival
under
conditioned
medium
from
hemin-insulted
neurons
improving
capacities
Collectively,
our
investigation
suggested
effectively
enhanced
effects
ICH
upregulating
Curcumin-preconditioned
be
taken
as
novel
strategy
Medical Gas Research,
Journal Year:
2021,
Volume and Issue:
12(3), P. 73 - 76
Published: Nov. 19, 2021
Cerebral
ischemia/reperfusion
injury
is
an
important
factor
leading
to
poor
prognosis
in
ischemic
stroke
patients.
Therefore,
it
particularly
find
effective
remedial
measures
promote
the
health
of
patients
return
society.
Isoflurane
a
safe
and
reliable
anesthetic
gas
with
long
history
clinical
application.
In
recent
years,
its
protection
function
human
body
has
been
widely
recognized,
nowadays
isoflurane
for
cerebral
studied,
stable
effect
satisfied
many
researchers.
Basic
studies
have
shown
that
isoflurane's
brain
tissue
after
involves
variety
signaling
pathways
effector
molecules.
Even
though
described,
more
focus
on
exploring
their
mechanisms
action,
order
provide
strong
evidence
This
could
prompt
introduction
therapy
as
soon
possible.
this
paper,
several
confirmed
will
be
reviewed
possible
strategies
treatment.
Brain Sciences,
Journal Year:
2022,
Volume and Issue:
12(5), P. 631 - 631
Published: May 11, 2022
Mesenchymal
stem
cells
(MSCs)
have
great
application
prospects
in
the
treatment
of
ischemic
injury.
However,
their
long-time
cultivation
before
transplantation
and
poor
survival
after
greatly
limit
therapeutic
effect
applications.
This
study
aimed
to
investigate
whether
MSCs
under
microenvironment
could
improve
better
alleviate
cerebral
Firstly,
we
used
brain
tissue
culture
evaluated
functional
changes
MSCs.
Then
a
middle
artery
occlusion
(MCAO)
model
was
induced
rats,
pretreated
were
injected
via
tail
vein.
The
adhesive
removal
test,
rotarod
modified
neurological
severity
score,
pathological
analyses
applied
assess
rats'
function.
expression
neuron
apoptosis
related
markers
detected.
results
indicated
that
promoted
proliferation
release
growth
factors
Meanwhile,
MCAO
enhanced
neurogenesis,
attenuated
behavioral
changes,
reduced
infarct
size,
inhibited
apoptosis.
B-cell
lymphoma-2
(Bcl-2),
brain-derived
neurotrophic
factor
(BDNF),
glial
fibrillary
acidic
protein
(GFAP),
NF-L,
NeuN
increased,
while
BCL2-Associated
X
(Bax)
Caspase-3
decreased.
Our
suggest
pretreatment
with
stroke
be
an
effective
strategy
treating
