Compound 21 Attenuates Isoflurane-Induced Injury in Neonatal Rat Hippocampal Neurons and Primary Rat Neuronal Cells by Upregulating METTL3 DOI Creative Commons
Yaping Shen, Yongjian Wu,

Hai Zhuang

et al.

Journal of Inflammation Research, Journal Year: 2024, Volume and Issue: Volume 17, P. 10079 - 10091

Published: Nov. 1, 2024

Isoflurane, as an anesthetic drug, has a neurotoxic effect on the developing brain tissue. Compound 21 (C21) been reported to be neuroprotective and ameliorate stroke effects. However, mechanism by which C21 protects against nerve damage remains unclear. Animal cellular models of injury were constructed using isoflurane (ISO) in neonatal SD rats primary rat neuronal cells (PRNCs). After treatment with C21, ultrastructure morphology hippocampus model assessed transmission electron microscope H&E staining. Methylation or apoptosis-related genes proteins examined immunohistochemistry, RT-qPCR, Western blot. The levels inflammatory factors monitored ELISA kits. m6A modification was analyzed Dot blot MeRIP-qPCR. Cell proliferation apoptosis also tested Edu TUNEL suppresses inflammation improves hippocampal ISO-induced rats. Mechanistically, upregulates modification, PPAR-a, BCL-2, METTL3 ISO-treated PRNCs. promotes cell proliferation, enhances BCL-2 reduces upregulating These findings suggest that survival up-regulates methylation Bc1-2 levels, potentially offering therapeutic strategy for neuroprotection clinical settings, particularly cases exposure. may related upregulation METTL3.

Language: Английский

Activation of the CD200/CD200R1 axis improves cognitive impairment by enhancing hippocampal neurogenesis via suppression of M1 microglial polarization and neuroinflammation in hypoxic-ischemic neonatal rats DOI Creative Commons
Haitao Qian, Andi Chen,

Daoyi Lin

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 128, P. 111532 - 111532

Published: Jan. 21, 2024

Following hypoxic-ischemic brain damage (HIBD), there is a decline in cognitive function; however, are no effective treatment strategies for this condition neonates. This study aimed to evaluate the role of cluster differentiation 200 (CD200)/CD200R1 axis function following HIBD using an established model postnatal day 7 rats. Western blotting analysis was conducted protein expression levels CD200, CD200R1, proteins associated with PI3K/Akt-NF-κB pathway, and inflammatory factors such as TNF-α, IL-1β, IL-6 hippocampus. Additionally, double-immunofluorescence labeling utilized M1 microglial polarization neurogenesis To assess learning memory experimental rats, Morris water maze (MWM) test conducted. leads decrease CD200 CD200R1 neonatal rat hippocampus, while simultaneously increasing IL-6, IL-1β proteins, ultimately resulting impairment. The administration CD200Fc, fusion found enhance p-PI3K p-Akt, but reduce p-NF-κB. CD200Fc inhibited microglia, reduced neuroinflammation, improved hippocampal neurogenesis, mitigated impairment caused by In contrast, blocking interaction between anti-CD200R1 antibody (CD200R1 Ab) exerted opposite effect. Furthermore, PI3K specific activator, 740Y-P, significantly increased p-NF-κB expression. It also rats HIBD. Our findings illustrate that activation CD200/CD200R1 inhibits NF-κB-mediated microglia improve HIBD-induced disorder via PI3K/Akt signaling pathway.

Language: Английский

Citations

9
Cav3.2 channel regulates cerebral ischemia/reperfusion injury: a promising target for intervention DOI Creative Commons
Feibiao Dai, Chengyun Hu, Xue Li

et al.

Neural Regeneration Research, Journal Year: 2023, Volume and Issue: 19(11), P. 2480 - 2487

Published: Dec. 14, 2023

JOURNAL/nrgr/04.03/01300535-202411000-00028/figure1/v/2024-04-10T160327Z/r/image-tiff Calcium influx into neurons triggers neuronal death during cerebral ischemia/reperfusion injury. Various calcium channels are involved in Cav3.2 channel is a main subtype of T-type channels. blockers, such as pimozide and mibefradil, have been shown to prevent injury-induced brain However, the role injury remains unclear. Here, vitro vivo models were established using middle artery occlusion mice high glucose hypoxia/reoxygenation exposure primary hippocampal neurons. The results showed that expression was significantly upregulated injured tissue We further gene-knockout mouse model knockout markedly reduced infarct volume water content, alleviated neurological dysfunction after Additionally, attenuated oxidative stress, inflammatory response, apoptosis. In hippocampus Cav3.2-knockout mice, calcineurin overexpression offset beneficial effect These findings suggest neuroprotective function mediated by calcineurin/nuclear factor activated T cells 3 signaling. Findings from this study could be promising target for treatment

Language: Английский

Citations

13
Emergency management of intracerebral haemorrhage in hypertensive, alkalotic patients: A case report DOI Creative Commons
Made Suandika, Rahmaya Nova Handayani,

Sekar Mutiara Triya Suryani

et al.

BIO Web of Conferences, Journal Year: 2025, Volume and Issue: 152, P. 01015 - 01015

Published: Jan. 1, 2025

The high incidence of neurosurgical cases involving anaesthesia poses a challenge for anaesthesiologists. This study is type case with an observational descriptive design in single which aims to provide holistic view and description interventions surgery intubation. novelty on the surgical management emergency craniotomy patient intracerebral haemorrhage accompanied by hypertension metabolic alkalosis. results obtained patients focus reducing intra cranial pressure, expanding haematoma, maintaining cerebral homeostatis, managing haemodynamic stability administering appropriate induction doses postoperative care monitoring propofol sedation improving quality controlled sleep intensive unit. conclusion this need well-coordinated perioperative anaesthetic considerations so that can be successful avoid excessive post-anesthetic complications.

Language: Английский

Citations

0
Free Radical-Induced Diseases DOI
Biswajit Mukherjee,

Sandipan Mallick,

Sanchari Bhattacharya

et al.

Dietary Supplements and Nutraceuticals, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 38

Published: Jan. 1, 2025

Language: Английский

Citations

0
Determinants of Outcome After Endovascular Middle Cerebral Artery Occlusion in Rats in the SPAN Trial DOI
Xuyan Jin, Andreia Morais, Takahiko Imai

et al.

Stroke, Journal Year: 2025, Volume and Issue: unknown

Published: May 21, 2025

BACKGROUND: The SPAN (Stroke Preclinical Assessment Network) is a confirmatory trial platform to test the efficacy and safety of candidate cerebroprotective interventions in acute stroke. As largest multicenter preclinical stroke date, SPAN1 (first SPAN) prospectively captured many biological procedural variables, revealing high degree heterogeneity introduced by approach that may impact outcomes. Here, we examined predictors tissue neurological outcomes after focal cerebral ischemic rats. METHODS: enrolled randomized 698 rats various active treatment arms or controls. Rats were subjected transient middle artery occlusion for 60 (spontaneously hypertensive rats) 120 minutes (young, healthy Sprague-Dawley followed 1 month. Nine independent variables (sex, weight, strain, intervention arm, site, endovascular filament silicone tip coating characteristics, anesthesia duration, protocol) 5 dependent outcome (weight loss, 4-point neuroscore, corner test, infarct volume, mortality) captured. Multivariable regression was used identify each readout determine their effect sizes. RESULTS: Spontaneously exhibited larger infarcts than rats, particularly among females. Neuroscores also worse spontaneously Prolonged exposure associated with smaller cortical hippocampal infarcts. Filament thickness length showed complex association different regional volumes, neuroscores, weight Mortality Bivariate analysis revealed moderate correlations CONCLUSIONS: Using large multicenter, prospective dataset, our multivariable analyses identified several influencing rat refuted others previously reported. Investigators should consider whether herein be standardized, accounted for, stratified during subject allocation decrease variability avoid confounders future trials.

Language: Английский

Citations

0
Emerging diagnostic markers and therapeutic targets in post-stroke hemorrhagic transformation and brain edema DOI Creative Commons

Ying Yao,

Fei Liu,

Zhaowen Gu

et al.

Frontiers in Molecular Neuroscience, Journal Year: 2023, Volume and Issue: 16

Published: Dec. 21, 2023

Stroke is a devastating condition that can lead to significant morbidity and mortality. The aftermath of stroke, particularly hemorrhagic transformation (HT) brain edema, significantly impact the prognosis patients. Early detection effective management these complications are crucial for improving outcomes in stroke This review highlights emerging diagnostic markers therapeutic targets including claudin, occludin, zonula occluden, s100β, albumin, MMP-9, MMP-2, MMP-12, IL-1β, TNF-α, IL-6, IFN-γ, TGF-β, IL-10, IL-4, IL-13, MCP-1/CCL2, CXCL2, CXCL8, CXCL12, CCL5, CX3CL1, ICAM-1, VCAM-1, P-selectin, E-selectin, PECAM-1/CD31, JAMs, HMGB1, vWF, VEGF, ROS, NAC, AQP4. clinical significance implications biomarkers were also discussed.

Language: Английский

Citations

5
An update on the molecular mechanism and pharmacological interventions for Ischemia-reperfusion injury by regulating AMPK/mTOR signaling pathway in autophagy DOI
Bin Tang,

Zhijian Luo,

Rong Zhang

et al.

Cellular Signalling, Journal Year: 2023, Volume and Issue: 107, P. 110665 - 110665

Published: March 31, 2023

Language: Английский

Citations

4
2-Acetylacteoside improves recovery after ischemic stroke by promoting neurogenesis via the PI3K/Akt pathway DOI

Meng Wang,

Songyu Xing,

Yang Liu

et al.

Free Radical Biology and Medicine, Journal Year: 2024, Volume and Issue: 225, P. 415 - 429

Published: Oct. 11, 2024

Language: Английский

Citations

1
Bioinformatics Identification of angiogenesis-related biomarkers and therapeutic targets in cerebral ischemia-reperfusion DOI Creative Commons
Yonghong Wu, Jing Sun, Junhua Huang

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Dec. 30, 2024

Language: Английский

Citations

1
Effect of dexmedetomidine on liver transplantation: a meta-analysis DOI Creative Commons
Degong Jia, Shanshan Guo, Xinyi Wu

et al.

Frontiers in Pharmacology, Journal Year: 2023, Volume and Issue: 14

Published: May 22, 2023

Background: Dexmedetomidine (DEX), an adjuvant anesthetic, may improve the clinical outcomes of liver transplantation (LT). Methods: We summarized relevant trials DEX in patients undergoing LT. As 30 January 2023, we searched The Cochrane Library, MEDLINE, EMBASE, Clinical Trial.gov and WHO ICTRP. main were postoperative renal function. random effect model or fixed was used to summarize across centers based on differences heterogeneity. Results: meta-analysis included nine studies total. Compared with control group, group had a reduced warm ischemia time (MD-4.39; 95% CI-6.74--2.05), improved (peak aspartate transferase: MD-75.77, CI-112.81--38.73; peak alanine MD-133.51, CI-235.57--31.45) function creatinine: MD-8.35, CI-14.89--1.80), risk moderate-to-extreme ischemia-reperfusion injury (OR 0.28, CI 0.14-0.60). Finally, hospital stay these decreased (MD-2.28, CI-4.00--0.56). Subgroup analysis prospective showed that have better efficacy living donors adult recipients. Conclusion: can short-term shorten patients. However, long-term its interfering factors deserves further study. Systematic Review: identifier CRD42022351664.

Language: Английский

Citations

3