Contribution of semen to early embryo development: fertilization and beyond

Human Reproduction Update, Journal Year: 2023, Volume and Issue: 29(4), P. 395 - 433

Published: March 7, 2023

Abstract BACKGROUND It has long been thought that the factors affecting embryo and foetal development were exclusively maternally derived; hence, if issues regarding fertility to arise, blame traditionally placed solely on mother. An escalating interest in how paternal influence development, however, begun prove otherwise. Evidence suggests both seminal plasma (SP) sperm contribute multiple shape embryogenesis. This review thus focuses role semen driving early embryonic describes factors, such as SP, centriole, proteins, RNA, DNA, its integrity, together with epigenetics, may female reproductive tract post-fertilization events. The important contributions of highlight imperative need for further research this area, which is sure bring forth breakthroughs leading improvements infertility diagnosis ART well reducing risk miscarriage. OBJECTIVE AND RATIONALE provides a comprehensive overview human embryo, aim providing better understanding SP divisions, gene protein expression, miscarriage, congenital diseases. SEARCH METHODS PubMed searches performed using terms ‘sperm structure’, ‘capacitation’, ‘acrosome reaction’, ‘fertilization’, ‘oocyte activation’, ‘PLCζ’, ‘PAWP’, ‘sperm-borne oocyte activation factor’, deficiency’, centriole’, transport’, mitochondria’, ‘seminal plasma’, epigenetics’, histone modifications’, DNA methylation’, ‘sperm-derived transcripts’, proteins’, fragmentation’, mRNA’, miRNAs’, piRNAs’, aneuploidy’. reviewed articles restricted those published English between 1980 2022. OUTCOMES data suggest male-derived much more than just male haploid genome embryo. indicates contributes help fate These include from RNA integrity. In addition, epigenetic changes have an impact tract, fertilization, stages development. For example, recent proteomic transcriptomic studies identified several sperm-borne markers play roles fertilization WIDER IMPLICATIONS highlights are required work tandem counterparts allow correct A deeper shuttled over cell can shed light improve andrological perspective. Further aid preventing passing genetic abnormalities origin, decreasing incidence factor infertility. exact mechanisms contribution assist scientists IVF clinicians determining new causes recurrent miscarriage or failure.

Language: Английский

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The Renaissance of Male Infertility Management in the Golden Age of Andrology

The World Journal of Men s Health, Journal Year: 2023, Volume and Issue: 41(2), P. 237 - 237

Published: Jan. 1, 2023

Infertility affects nearly 186 million people worldwide and the male partner is cause in about half of cases. Meta-regression data indicate an unexplained decline sperm concentration total count over last four decades, with increasing prevalence infertility. This suggests urgent need to implement further basic clinical research Andrology. Andrology developed as a branch urology, gynecology, endocrinology, and, dermatology. The first scientific journal devoted andrological sciences was founded 1969. Since then, despite great advancements, andrology has encountered several obstacles its growth. In fact, for cultural reasons, often been neglected diagnostic therapeutic workup infertile couple. Furthermore, development assisted reproductive techniques (ART) driven strong impression that this biotechnology can overcome all forms infertility, common belief having spermatozoon from (a sort donor) needed achieve pregnancy. However, practice shown quality gamete important successful ART outcome. safety questioned because high comorbidities offspring conceptions compared spontaneous conceptions. These issues have paved way more greater understanding mechanisms spermatogenesis Consequently, numerous discoveries made field andrology, ranging genetics "omics" technologies, oxidative stress DNA fragmentation, sixth edition WHO manual, artificial intelligence, management azoospermia, fertility cancers survivors, testis, 3D printing, gene engineering, stem cells therapy spermatogenesis, reconstructive microsurgery seminal microbiome. Nevertheless, many cases infertility remain idiopathic, studies are required improve males. A multidisciplinary strategy involving both clinicians scientists basic, translational, core principle will allow limits reach goals. state-of-the-art article aims present historical review particularly, "Middle Ages" "Renaissance", golden age andrology.

Language: Английский

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How Relevant are Male Factors for Fertilization and Early Embryo Development? Looking into the (Epi)genome, Proteome and Metabolome

European Review, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 33

Published: Jan. 31, 2025

Infertility affects 10–15% of couples at the age conception. Mounting evidence supports that not only are paternal factors crucial during fertilization, but also for embryogenesis. This review aims to provide some clues about contribution male reproductive success and live birth, as such contributions can be important female. Semen is composed two fractions: sperm seminal plasma. Regarding former, integrity components (i.e., centrioles, DNA methylation, histone-to-protamine ration, specific proteins, etc.) has been proven essential events occurring upon engulfment spermatozoon into oocyte cytoplasm. The metabolic status seems shape their potential fertilizing capacity. Furthermore, plasma appears modulate female tract, suggested support embryo implantation. In spite aforementioned, it remains largely unaddressed how interact with maternal ones, whether latter may mask former. While assisted techniques (ART) useful rescue infertility, a better understanding semen development implantation increase efficiency these techniques, address further causes total fertilization failure, deficiency recurrent miscarriage.

Language: Английский

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Spermatozoa centriole quality determined by FRAC may correlate with zygote nucleoli polarization—a pilot study

Journal of Assisted Reproduction and Genetics, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 7, 2025

Spermatozoa centriolar defects can result in abnormal zygote functions. Recently, a method to quantify spermatozoa was developed named Fluorescence-Based Ratiometric Analysis of Sperm Centrioles (FRAC). However, whether identified by FRAC functions not tested. Here, we quantified using FRAC, and centriole function assessed imaging Nucleolus Precursor Body (NPB) polarization which based on the pattern NPB polarization. Data analyzed at couple embryo levels. Subjects were divided into two groups: seven couples 62 embryos with normal centrioles versus eight 78 (140 from 15 total). Patterned statistically significant both couple- embryo-level analyses (p < 0.0001 p = 0.0024). These results suggest that may correlate centrosome via scoring. This study provides foundation for more extensive studies test FRAC's utility assessing quality.

Language: Английский

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The proximal centriole-like structure maintains nucleus-centriole architecture in sperm

Journal of Cell Science, Journal Year: 2024, Volume and Issue: 137(17)

Published: Aug. 22, 2024

ABSTRACT Proper connection between the sperm head and tail is critical for motility fertilization. Head–tail linkage mediated by head-tail coupling apparatus (HTCA), which secures axoneme (tail) to nucleus (head). However, molecular architecture of HTCA poorly understood. Here, we use Drosophila investigate formation remodeling throughout spermiogenesis visualizing key components this complex. Using structured illumination microscopy, demonstrate that proteins Spag4 Yuri form a ‘centriole cap’ surrounds centriole (or basal body) as it invaginates into surface nucleus. As development progresses, laterally displaced side while expands under nucleus, forming what term ‘nuclear shelf’. We next show proximal centriole-like (PCL) structure positioned nuclear shelf, functioning crucial stabilizer centriole–nucleus attachment. Together, our data indicate complex, multi-point attachment site simultaneously engages PCL, ensure proper head–tail during late-stage spermiogenesis.

Language: Английский

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Sperm centriole assessment identifies male factor infertility in couples with unexplained infertility – a pilot study

European Journal of Cell Biology, Journal Year: 2022, Volume and Issue: 101(3), P. 151243 - 151243

Published: May 27, 2022

Unexplained infertility affects about one-third of infertile couples and is defined as the failure to identify cause despite extensive evaluation male female partners. Therefore, there a need for multiparametric approach study sperm function. Recently, we developed Fluorescence-Based Ratiometric Analysis Sperm Centrioles (FRAC) assay determine centriole quality. Here, perform pilot from 10 fertile men in with unexplained infertility, using three centriolar biomarkers measured at locations two fractions, representing high low We found that FRAC can likely source infertility. Higher quality fractions individuals were reference population. All 180 studied values fell within population range. Eleven patients outliers beyond 95% confidence intervals (P = 0.0008). Three had outlier their higher fraction, while four lower fraction (3/10 4/10, P 0.060 0.025, respectively), suggesting these are due, part, defects. propose larger scale should be performed ability factor its potential contribution analysis.

Language: Английский

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High-throughput proteomic characterization of seminal plasma from bulls with contrasting semen quality

3 Biotech, Journal Year: 2023, Volume and Issue: 13(2)

Published: Jan. 26, 2023

Language: Английский

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CEP112 coordinates translational regulation of essential fertility genes during spermiogenesis through phase separation in humans and mice

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Sept. 30, 2024

Language: Английский

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Focus on centrin in normal and altered human spermatozoa

Systems Biology in Reproductive Medicine, Journal Year: 2023, Volume and Issue: 69(3), P. 175 - 187

Published: March 9, 2023

This review provides details on the role of centrin in human spermatozoa and various forms male infertility. Centrin is a calcium (Ca2+)-binding phosphoprotein that located centrioles – which are typical structures sperm connecting piece play key centrosome dynamics during morphogenesis as well zygotes early embryos spindle assembly. In humans, three different genes encoding isoforms have been discovered. 1, only one expressed spermatozoa, seems to be lost inside oocyte after fertilization. The characterized by presence numerous proteins including centrin, deserves particular attention because, it enriched maturation centrioles. normal sperm, 1 visible two distinct spots head–tail junction; however, some defective distribution altered. has studied humans animal models. Its mutations may lead several structural alterations, such serious defects connective and, subsequently, fertilization failure or incomplete embryonic development. However, effects these abnormalities fertility not fully studied. Because function appears important for reproductive success, additional studies needed bring medical benefits resolving cases idiopathic

Language: Английский

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The Proximal Centriole-Like Structure Anchors the Centriole to the Sperm Nucleus

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 15, 2024

Proper connection between the sperm head and tail is critical for motility fertilization. The link mediated by Head-Tail Coupling Apparatus (HTCA), which secures axoneme (tail) to nucleus (head). However, molecular architecture of HTCA not well understood. Here, we use

Language: Английский

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