Molecular Markers: A New Paradigm in the Prediction of Sperm Freezability

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(4), P. 3379 - 3379

Published: Feb. 8, 2023

For decades now, sperm cryopreservation has been a pillar of assisted reproduction in animals as well humans. Nevertheless, the success varies across species, seasons, and latitudes even within same individual. With dawn progressive analytical techniques field genomics, proteomics, metabolomics, new options for more accurate semen quality assessment have become available. This review summarizes currently available information on specific molecular characteristics spermatozoa that could predict their cryotolerance before freezing process. Understanding changes biology result exposure to low temperatures may contribute development implementation appropriate measures assure high post-thaw quality. Furthermore, an early prediction or cryosensitivity lead establishment customized protocols interconnecting adequate processing procedures, techniques, cryosupplements are most feasible individual needs ejaculate.

Language: Английский

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Does the Type of Semen Affect the Phosphoproteome of Turkey (Meleagris gallopavo) Spermatozoa?

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3467 - 3467

Published: April 8, 2025

Yellow semen syndrome (YSS) is an increasingly common reproductive health problem in male turkeys. This condition characterised by a yellow discolouration of semen, often linked to decreased quality and fertility. poses significant concern due its negative impact on the performance Phosphorylation one major post-translational modifications proteins. A better understanding function sperm phosphoproteome crucial for advancement biology development therapies infertility. Spermatozoa from samples with YSS were lower levels malondialdehyde (MDA), reduced plasma membrane integrity (PMI), mitochondrial potential (MMP). However, these showed increased antioxidant enzyme activity elevated glutathione (GSH) content. also had percentage viable cells higher proportion apoptotic necrotic cells. The phosphoproteins identified turkey play key roles maturation, functional motility apparatus, efficient cellular metabolism, protection against oxidative stress, successful fertilisation egg. altered phosphorylation proteins serine, threonine (p ≤ 0.05), tyrosine residues, which could have influenced metabolism physiology spermatozoa samples, thus affecting their potential. These findings highlight function, including phosphorylation-dependent processes that are reproduction.

Language: Английский

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Seminal Plasma Proteomics Using Filter-Aided Sample Preparation

Methods in molecular biology, Journal Year: 2025, Volume and Issue: unknown, P. 637 - 645

Published: Jan. 1, 2025

Language: Английский

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Comprehensive proteomic characterization and functional annotation of Siberian sturgeon seminal plasma proteins

Aquaculture, Journal Year: 2023, Volume and Issue: 568, P. 739326 - 739326

Published: Feb. 8, 2023

Language: Английский

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Rab2 and Rab6 are Implicated in Acrosome Formation during Spermatogenesis in Eriocheir sinensis: Based on Sperm Proteome

Frontiers in Bioscience-Landmark, Journal Year: 2023, Volume and Issue: 28(8)

Published: Aug. 7, 2023

Background: Rab proteins are GTP-dependent small that function as regulators of intracellular vesicle transport, fusion, and localization. However, few studies have investigated their in Decapoda reproduction. The Eriocheir sinensis sperm has no tail the nuclei uncondensed. With acrosome forming majority mass, it provides an ideal model for studying formation. Methods: We firstly analyzed proteome using LC-MS/MS. To study functions Rab2 Rab6, related to Golgi apparatus, formation during spermatogenesis, genes Rab6 were cloned based on testis transcriptome E.sinensis poly-clonal antibodies prepared. presence 2 was confirmed by western blot. further observed characteristics target immunofluorescence (IF). Results: A total 1247 including 7 proteins, Rab1, Rab2, Rab5, Rab11, Rab14, Rab18 identified proteome. IF results showed co-localizes with GM130, a cis-Golgi matrix protein, spermatagonia spermatocytes. In early spermatids, participate pre-acrosomal vesicles. maturing both settle acrosomal membrane but present different wrapping pre-acrosome. mature sperm, localizes perinuclear theca surrounding cup, while remains membrane. Conclusions: Our research found analysis E.sinensis, involvement These findings provide foundation spermatogenesis animals.

Language: Английский

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Proteomic Insights into Seminal Plasma and Spermatozoa Proteins of Small-Spotted Catsharks, Scyliorhinus canicula: Implications for Reproductive Conservation in Aquariums

Animals, Journal Year: 2024, Volume and Issue: 14(9), P. 1281 - 1281

Published: April 24, 2024

In the ex situ conservation of chondrichthyan species, successful reproduction in aquaria is essential. However, these species often exhibit reduced reproductive success under human care. A key aspect that conventional sperm analyses do not provide insights into functional competence sperm. proteomics analysis enables a better understanding male physiology, gaining relevance as powerful tool for discovering protein biomarkers related to fertility. The present work aims build first proteome database shark semen and investigate proteomic profiles seminal plasma spermatozoa from small-spotted catsharks (Scyliorhinus canicula) underlying adaptations both natural aquarium environments, thereby identifying impact specimens. total 305 535 proteins were identified. Among these, 89 (29.2% set) common plasma. plasma, only adenosylhomocysteinase showed differential abundance (DAP) between wild animals. With respect proteins, 107 DAPs found groups. Gene Ontology enrichment highlighted primary roles involved oxidoreductase activity. Additionally, KEGG indicated primarily associated with metabolic pathways carbon metabolism. conclusion, we have successfully generated an initial S. canicula spermatozoa. Furthermore, identified variations, predominantly within spermatozoa, populations canicula. These findings foundation future biomarker discovery studies. additional research required determine whether variations correlate declines captive sharks.

Language: Английский

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ShinySperm: navigating the sperm proteome landscape

Reproduction Fertility and Development, Journal Year: 2024, Volume and Issue: 36(13)

Published: Aug. 1, 2024

Context Integrated omics studies hold a crucial role in improving our understanding of reproductive biology. However, the complex datasets so generated are often only accessible via supplementary data files, which lack capacity for interactive features to allow users readily interrogate and visualise interest. Aims The intent this technical note was develop an web-based application that enables detailed interrogation representative sperm proteome, facilitating deeper proteins identified, their relative abundance, classifications, functions, associated phenotypes. Methods We developed Shiny web application, ShinySperm (https://reproproteomics.shinyapps.io/ShinySperm/), utilising R several complementary libraries manipulation (dplyr), tables (DT), visualisation (ggplot2, plotly). responsive user interface, dynamic filtering options, charts, export capabilities. Key results allows interactively search, filter, proteomics based on key (e.g. protein classification, cell domain, presence, or absence at different maturation stages). This responds live options generation plots tables, thus enhancing engagement data. Conclusions provides robust platform exploration epididymal proteome It significantly improves accessibility interpretability datasets, allowing effective data-driven insights. Implications highlights potential applications biology plug play script field produce meaningful researcher interaction with omic datasets.

Language: Английский

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Seminal Plasma Proteins as Biomarkers of Fertility and Semen Traits in Egyptian Barki Rams

Annals of Agricultural Science Moshtohor, Journal Year: 2022, Volume and Issue: 60(3), P. 787 - 798

Published: Sept. 1, 2022

The objective of this study was to identify seminal plasma proteins associated with semen traits (mass motility (MM; %), live sperm (LS; abnormal (AS; hypo-osmotic swelling test (Host; pH, colour, viscosity (Visco), ejaculate volume (VOL; ml), normal chromatin (NC; reacted acrosome (LR; unreacted (LU; dead (DR; (DU; %)) in Egyptian Barki rams. Depending on MM%, the animals were divided into three different groups (high (≥ 80% MM), medium (50-80% MM) and low-fertility (≤50% group). separated using one-dimensional sodium dodecyl sulfate polyacrylamide gel (SDS-PAGE) electrophoresis technique based their molecular weight. actual means for MM, LS, AS, Host, Visco, VOL, NC, LR, LU, DR DU 69.93%, 94.40%, 6.76%, 20.14%, 6.94, 3.30, 3.04, 0.75 mL, 53.52%, 44%, 15.78%, 19.36% 20.86%, respectively. correlation coefficients between MM each LS (0.61) NC (0.89) highly positive significant (P<0.05). Semen samples displayed a total 8 distinct protein bands SDS-PAGE proteins. identified ranged weight from 14 248 kDa. high fertility ram showed six bands. However, all eight seen group It is notable that two solely are those weights 29 35 Negative correlations (P<0.05) observed these -0.87 -0.36 kDa band -0.62 -0.56 band, reduced low rams may be caused by protein. In contrast, there kDa, 25 LR Relevant mass other characteristics found Potential have as biomarkers related traits.

Language: Английский

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Research Note: Spermatozoa proteins identification in domesticated pigeons by proteomic analysis

Poultry Science, Journal Year: 2023, Volume and Issue: 102(11), P. 103088 - 103088

Published: Sept. 3, 2023

Proteins are considered major effectors of sperm function. However, the proteins expressed in pigeon have not been explored. Here, we collected semen from meat and racing pigeons using electroejaculation method identified proteomics approach. A total 1,641 were domesticated pigeons. Of which, 1,541 reliably quantified, gene ontology (GO) associated bioinformatics analyses indicated that annotated linked to oxidation-reduction process, integral component membrane, protein binding, etc. Among quantified proteins, 1,515 1,507 respectively presented White King pigeons, 1,481 shared between these 2 types including axonemal dynein, solute carrier, cilia- flagella-associated protein, outer dense fiber our constructed protein-protein interaction (PPI) network involved oxidative phosphorylation, axoneme assembly, cilium-dependent cell motility, dynein complex flagellated In conclusion, this study characterized proteome provided a foundation for subsequent research screening markers fertility evaluation

Language: Английский

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Effect of Carbohydrate Type and Phenotype on the Quality of Post-Thawing Frozen Semen of KUB Chicken

Jurnal Ilmu Ternak dan Veteriner, Journal Year: 2023, Volume and Issue: 28(1), P. 56 - 68

Published: Feb. 2, 2023

The superior Balitbangtan Kampung Chicken (KUB) chickens have different phenotypes. It was reported that the chicken phenotype related to semen quality. This study aimed determine post-thawing characteristics and quality of KUB with phenotypes frozen in Ringer's lactate egg yolk (RLEY) diluent addition fructose or glucose. Semen collected using massaging method from 20 a single comb black dark brown feather color red neck (SCNR), green-black white collar (SCNW), pea feathers fur (PCNR), (PCNW). each divided into three parts types diluents: RLEY, RLEY+fructose (RLEYF), RLEY+glucose (RLEYG). highest sperm motility found glucose SCNR PCNW (P0.05). viability shown RLEYG abnormality RLEY RLEYF diluents SCNW, PCNR, groups, whereas group, it only PCNR group. From results this study, can be concluded type influences semen. best is attachments phenotype.

Language: Английский

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