Livestock diseases and management, Journal Year: 2024, Volume and Issue: unknown, P. 699 - 741
Published: Jan. 1, 2024
Language: Английский
ACS Omega, Journal Year: 2024, Volume and Issue: 9(12), P. 13534 - 13555
Published: March 7, 2024
Pulmonary diseases like asthma, chronic obstructive pulmonary disorder, lung fibrosis, and cancer pose a significant burden to global human health. Many of these complications arise as result exposure particulate matter (PM), which has been examined in several preclinical clinical trials for its effect on respiratory diseases. Particulate size less than 2.5 μm (PM2.5) known inflict unforeseen repercussions, although data from epidemiological studies back this are pending. Conventionally utilized two-dimensional (2D) cell culture animal models have provided insufficient benefits emulating the vivo physiological pathological conditions. Three-dimensional (3D) structural models, including organ-on-a-chip experienced developmental upsurge recent times. Lung-on-a-chip potential simulate specific features lungs. With advancement technology, an emerging advanced technique termed microfluidic developed with aim identifying complexity cellular microenvironment body. In present Review, role lung-on-a-chip modeling reproducing explored, emphasis PM2.5-induced complications.
Language: Английский
Citations
6
Nanomaterials, Journal Year: 2022, Volume and Issue: 12(11), P. 1810 - 1810
Published: May 25, 2022
The use of nanomaterials has been increasing in recent times, and they are widely used industries such as cosmetics, drugs, food, water treatment, agriculture. rapid development new demands a set approaches to evaluate the potential toxicity risks related them. In this regard, nanosafety using adapting already existing methods (toxicological approach), but unique characteristics demand (nanotoxicology) fully understand toxicity, immunotoxicity, (epi)genotoxicity. addition, technologies, organs-on-chips sophisticated sensors, under and/or adaptation. All information generated is develop silico trying predict effects newly developed materials. overall evaluation from their production final disposal chain completed life cycle assessment (LCA), which becoming an important element considering sustainability environmental impact. review, we give overview all these elements nanosafety.
Language: Английский
Citations
24
Acta Biomaterialia, Journal Year: 2022, Volume and Issue: 146, P. 10 - 22
Published: May 4, 2022
Language: Английский
Citations
19
EFSA Supporting Publications, Journal Year: 2024, Volume and Issue: 21(9)
Published: Sept. 1, 2024
Abstract New Approach Methodologies (NAMs), broadly understood to include in silico, chemico, vitro and ex vivo methods, show great potential advancing risk assessment albeit their regulatory implementation is lagging. The EFSA Guidance on of nanomaterials (EFSA Nano‐RA) suggests nano‐specific best achieved through Integrated Approaches Testing Assessment (IATAs) with NAMs as the first choice generate new information. Integrating promises several advantages such a better human focus, more detailed insights into molecular mechanisms higher efficacy. However, applying NMs also poses considerable challenges issues related dispersion stability, dosimetry, agglomeration, dissolution, transformations or assay interferences. Significant efforts are being undertaken by standardisation organisations research projects establish various for NMs. Here thorough review provided covering that will be potentially useful food feed sector. It follows structure Nano‐RA expands it, where needed, support decision‐making selection NM assessment. begins an overview NAM‐frameworks, followed description individual including those relevant physicochemical characterisation, exposure hazard toxicodynamics toxicokinetics. focus concerning degradation/dissolution, genotoxicity, cytotoxicity, oxidative stress, (pro‐)inflammation, barrier integrity important endpoints initial screening according framework. As result, total 267 nano‐relevant NAMs, mostly “not validated” (with few notable exceptions), were included this review. Validation notwithstanding, could already prove reliable NMs, especially integrated approaches.
Language: Английский
Citations
4
Frontiers in Materials, Journal Year: 2023, Volume and Issue: 10
Published: April 6, 2023
Congenital and acquired tissular losses due to disease or trauma are a major world health problem. Regenerative therapy aims fix damaged tissues by directing the natural capacity of host organism use biofunctionalized artificial tissue scaffolds. These three-dimensional (3D) scaffolds can be customized with cells and/or bioactive molecules induce cellular homing angiogenesis, essential ensure successful regeneration. Hydrogels (HGs) networks hydrophilic homopolymers, copolymers, macromers chemical biological activities that enhance their cell colonization. The HGs in regenerative medicine has shown advantageous since prepared under clinical-grade conditions tailored specific needs replaced tissue. They made emulate native extracellular matrices (ECMs) including physical, mechanical, cues resilience properties. reproduce hygroscopic original which improves anchoring, nutrition, waste disposal. enable molecular modification conducive microenvironment, modifying properties scaffold, improving chemotaxis, adhesion, migration, proliferation, differentiation, angiogenesis; created linked growth factors synthetic peptides positively influence scaffold colonization functional biocompatibility. This review collect most relevant information regarding biofunctionalization used for vascular regeneration, effects, clinical implications. While still investigation, some them have been studied vitro, ex vivo , promising results . In this regard, studies such as chitosan hydrogel LL-37 promotes angiogenesis healing pressure ulcers. Also, GHK tripeptide is widely trials focused on guided remodeling.
Language: Английский
Citations
7
Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)
Published: April 15, 2023
Abstract Kidneys are complex organs, and reproducing their function physiology in a laboratory setting remains difficult. During drug development, potential compounds may exhibit unexpected nephrotoxic effects, which imposes significant financial burden on pharmaceutical companies. As result, there is an ongoing need for more accurate model systems. The use of renal organoids to simulate responses insults has the bridge gap between preclinical efficacy studies cell cultures animal models, stages clinical trials humans. Here we established accessible fluorescent whole-mount approach nuclear membrane staining first provide overview organoid histology. Furthermore, investigated toxicity. For this purpose, were treated with chemotherapeutic agent doxorubicin 48 h. When viability was assessed biochemically, demonstrated significant, dose-dependent decline response treatment. Confocal microscopy revealed visible tubular disintegration loss cellular boundaries at high concentrations. This observation further reinforced by decrease area analyzed images. In contrast other approaches, study, straightforward experimental framework toxicity assessment that be used early research assist screen adverse effects compounds.
Language: Английский
Citations
7
Frontiers in Veterinary Science, Journal Year: 2023, Volume and Issue: 10
Published: Oct. 18, 2023
Organoids are in vitro organ-like structures that faithfully recapitulate many characteristics of a specific organ. During the past decades, major progress has been accomplished establishing three-dimensional (3D) culture systems toward stem cell-derived organoids. As significant technological breakthrough, these amazing 3D organoid constructs bridge conventional 2D models and vivo animal provide an unprecedented opportunity to investigate complexities veterinary diseases ranging from their pathogenesis prevention, therapy, or even future organ replacement strategies. In this review, we briefly discuss several definitions used research highlight currently known achievements modeling diseases, including infectious inflammatory cancers, metabolic diseases. The applications technology disease still infancy stage but is promising.
Language: Английский
Citations
7
ACS Applied Materials & Interfaces, Journal Year: 2022, Volume and Issue: 15(1), P. 200 - 219
Published: Dec. 5, 2022
Brain organoids are self-assembled three-dimensional aggregates with brain-like cell types and structures have emerged as new model systems that can be used to investigate human neurodevelopment neurological disorders. However, brain not mature functional real brains due limitations of the culture system insufficient developmental patterning signals a lack components important for development function, such non-neural population vasculature. In addition, establishing desired environment monitoring complex neural networks physiological functions remain challenging. The current protocols generate also problems heterogeneity batch variation spontaneous self-organization into architectures brain. To address these organoid technologies, various engineering platforms, extracellular matrices, fluidic devices, bioprinting, bioreactors, polymeric scaffolds, microelectrodes, biochemical sensors, been employed improve neuronal maturation, reduce structural heterogeneity, facilitate analysis monitoring. this review, we provide an overview latest techniques overcome in production application organoids.
Language: Английский
Citations
12
Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology, Journal Year: 2022, Volume and Issue: 15(1)
Published: Aug. 10, 2022
Abstract Recently, the aging population has increased exponentially around globe bringing more challenges to improve quality of life in those populations while reducing economic burden on healthcare systems. Aging is associated with changes immune system culminating detrimental effects such as dysfunction, immunosenescence, and chronic inflammation. Age‐related decline functions various pathologies including cardiovascular, autoimmune, neurodegenerative, infectious diseases name a few. Conventional treatment addresses onset age‐related by early detection risk factors, administration vaccines preventive care, immunomodulatory treatment, other dietary supplements. However, these approaches often come systemic side‐effects, low bioavailability therapeutic agents, poor outcomes seen elderly. Recent innovations nanotechnology have led development novel biomaterials/nanomaterials, which explore targeted drug delivery interactions vivo. Current nanotechnology‐based that potential be used interventions for some prominent are discussed here. Finally, we future aspects treatments disorders This article categorized under: Therapeutic Approaches Drug Discovery > Nanomedicine Cardiovascular Disease Neurological Emerging Technologies
Language: Английский
Citations
11
International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(22), P. 14361 - 14361
Published: Nov. 18, 2022
Efforts to heal damaged pulp tissue through engineering have produced positive results in pilot trials. However, the differentiation between real regeneration and mere repair is not possible clinical measures. Therefore, preclinical study models are still of great importance, both gain insights into treatment outcomes on cell levels develop further concepts for dental regeneration. This review aims at compiling information about different vitro vivo ectopic, semiorthotopic, orthotopic models. In this context, differences monolayer three-dimensional cultures discussed, a semiorthotopic transplantation model introduced as an regeneration, finally, animal used investigations presented.
Language: Английский
Citations
11