Advances in Clinical Medicine, Journal Year: 2023, Volume and Issue: 13(07), P. 11741 - 11749
Published: Jan. 1, 2023
Language: Английский
Bioactive Materials, Journal Year: 2022, Volume and Issue: 17, P. 344 - 359
Published: Jan. 22, 2022
Shoulder stiffness (SS) is a common shoulder disease characterized by increasing pain and limited range of motion. SS considered to be an inflammatory fibrotic disorder pathologically. However, there no consensus on the most effective conservative treatment for fibrosis. Given that human Bone Marrow Mesenchymal Stem Cell-derived extracellular vesicles (BMSC-EVs) displayed promising therapeutic effects various tissues, we investigated effect BMSC-EVs fibrosis in mice immobilization model two cell models. By conducting series experiments, found can significantly inhibit fibrogenic process both vitro vivo. In detail, suppressed aberrant proliferation, high collagen production capacity, activation pathways TGF-β-stimulated fibroblasts vitro. Besides, vivo, reduced infiltration, tissue capsule, improved mobility. addition, via exosomal small RNA sequencing qPCR analysis, let-7a-5p was verified highest expressed miRNA with predicted antifibrotic capability BMSC-EVs. The capacity impaired after knockdown let-7a-5p. Moreover, discovered mRNA TGFBR1 (the membrane receptor transforming growth factor β) target Together, these findings elucidated role capsular This study clarifies new approach using stem cell-derived EVs therapy as alternative therapy, which may clinically benefit patients future.
Language: Английский
Citations
65
Pharmaceutics, Journal Year: 2022, Volume and Issue: 14(5), P. 1012 - 1012
Published: May 8, 2022
Extracellular vesicles (EVs) are small membrane that can be secreted by most cells. EVs released into the extracellular environment through exocytosis, transporting endogenous cargo (proteins, lipids, RNAs, etc.) to target cells and thereby triggering release of these biomolecules participating in various physiological pathological processes. Among them, derived from bone marrow mesenchymal stem (BMSC-EVs) have similar therapeutic effects BMSCs, including repairing damaged tissues, inhibiting macrophage polarization promoting angiogenesis. In addition, BMSC-EVs, as efficient feasible natural nanocarriers for drug delivery, advantages low immunogenicity, no ethical controversy, good stability easy storage, thus providing a promising strategy many diseases. particular, BMSC-EVs show great potential treatment metabolic This article reviews mechanism formation resorption, which provides new insights future research on strategies
Language: Английский
Citations
52
International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 1345 - 1345
Published: Feb. 5, 2025
Exosomes are a subset of extracellular vesicles (EVs) secreted by nearly all cell types and have emerged as novel mechanism for intercellular communication within the central nervous system (CNS). These facilitate transport proteins, nucleic acids, lipids, metabolites between neurons glial cells, playing pivotal role in CNS development maintenance homeostasis. Current evidence indicates that exosomes from cells may function either inhibitors or enhancers onset progression neurological disorders. Furthermore, been found to disease-related molecules across blood–brain barrier, enabling their detection peripheral blood. This distinctive property positions promising diagnostic biomarkers conditions. Additionally, growing body research suggests derived mesenchymal stem exhibit reparative effects context review provides concise overview functions both physiological pathological states, with particular emphasis on emerging roles potential therapeutic agents treatment diseases.
Language: Английский
Citations
1
Frontiers in Cell and Developmental Biology, Journal Year: 2022, Volume and Issue: 9
Published: Jan. 14, 2022
Exosomes are extracellular vesicles formed by various donor cells that regulate gene expression and cellular function in recipient cells. derived from mesenchymal stem (MSC-Exos) perform the regulatory of transporting proteins, nucleic acids, lipids. Intervertebral disc degeneration (IDD) is one main causes low back pain, it characterized a decreased number nucleus pulposus cells, matrix decomposition, aging annulus fibrosus, cartilage endplate calcification. Besides, nutrient transport structural repair intervertebral discs depend on bone closely related to state bone. Trauma, disease can all cause injury. However, there lack effective drugs against IDD Recent MSC-Exos fine tuning has led significant progress treatment regeneration. In this review, we looked at uniqueness MSC-Exos, potential mechanisms with respect IDD, defects injuries.
Language: Английский
Citations
38
Bioactive Materials, Journal Year: 2024, Volume and Issue: 37, P. 1 - 13
Published: March 13, 2024
Low back pain is among the most grave public health concerns worldwide and major clinical manifestation of intervertebral disc degeneration (IVDD). The destruction annulus fibrosus (AF) primary cause IVDD. A sustainable stable treatment system for IVDD lacking because special organizational structure low nutrient supply AF. We here found that results in impaired mitochondrial function AF tissue, autophagy (mitophagy) plays a protective role this process. therefore reported thread-structural microneedle (T-MN) matching ring Based on adsorption effect laminin, our T-MN could load with bone marrow mesenchymal stem cell-derived exosomes to envelope regulating mitophagy microRNA (miRNA 378), named as
Language: Английский
Citations
7
Frontiers in Cell and Developmental Biology, Journal Year: 2022, Volume and Issue: 9
Published: Feb. 10, 2022
Low back pain (LBP), which is one of the most severe medical and social problems globally, has affected nearly 80% population worldwide, intervertebral disc degeneration (IDD) a common musculoskeletal disorder that happens to be primary trigger LBP. The pathology IDD based on impaired homeostasis catabolism anabolism in extracellular matrix (ECM), uncontrolled activation immunologic cascades, dysfunction, loss nucleus pulposus (NP) cells addition dynamic cellular biochemical alterations microenvironment (IVD). Currently, main therapeutic approach regarding surgical intervention, but it could not considerably cure IDD. Exosomes, vesicles with diameter 30–150 nm, are secreted by various kinds cell types like stem cells, tumor immune endothelial cells; lipid bilayer exosomes protects them from ribonuclease degradation helps improve their biological efficiency recipient cells. Increasing lines evidence have reported promising applications immunological diseases, regarded as potential source for This review focuses clarifying novel therapies derived different sources essential roles regulating IDD, especially strategy.
Language: Английский
Citations
23
Bioengineered, Journal Year: 2022, Volume and Issue: 13(5), P. 11933 - 11944
Published: May 2, 2022
Bone mesenchymal stem cells (BMSCs)-derived exosomes (Exos) play important roles in osteoporosis, while the regulation of microRNA (miR)-21-5p remains unclear. The BMSCs-derived were isolated from femoral bone marrow trauma patients, which then used to stimulate human osteoblasts (hFOB1.19 cells). miR-21-5p mimic or inhibitor was transfected into BMSCs overexpress knockdown miR-21-5p. functions osteoporosis assessed by cell counting kit-8 (CCK-8) assay, alkaline phosphatase (ALP) staining and alizarin red assays. We found that could enhance proliferation, osteoblastic differentiation ALP activity hFOB1.19 cells. with upregulated further these protective impacts compared exosomes, downregulated reduced phenotypes. MiR-21-5p directly bind 3'-untranslated region (UTR) Kruppel-like factor 3 (KLF3), KLF3 obviously attenuated inhibitory effects on In summary, exosomal improved through regulating KLF3, providing a potential therapeutic strategy for osteoporosis.
Language: Английский
Citations
23
World Journal of Diabetes, Journal Year: 2022, Volume and Issue: 13(12), P. 1066 - 1095
Published: Dec. 14, 2022
Chronic wound healing has long been an unmet medical need in the field of repair, with diabetes being one major etiologies. Diabetic chronic wounds (DCWs), especially diabetic foot ulcers, are most threatening complications diabetes. Although treatment strategies, drugs, and dressings for DCWs have made great progress, they remain ineffective some patients refractory wounds. Stem cell-based therapies achieved specific efficacy various fields, mesenchymal stem cells (MSCs) widely used. MSCs good feedback preclinical studies clinical trials cutaneous or other situations, potential safety concerns associated allogeneic/autologous unknown long-term health effects further attention supervision. Recent reported that mainly exert their trauma repair through paracrine secretion, exosomes play important role intercellular communication as main bioactive component. MSC-derived (MSC-Exos) inherit powerful inflammation immune modulation, angiogenesis, cell proliferation migration promotion, oxidative stress alleviation, collagen remodeling imbalances regulation parental cells, can avoid risks direct transplantation to a large extent, thus demonstrating promising performance novel "cell-free" This review aimed elucidate mechanism update progress MSC-Exos DCW healing, thereby providing new therapeutic directions difficult be cured using conventional therapy.
Language: Английский
Citations
21
Extracellular Vesicles and Circulating Nucleic Acids, Journal Year: 2024, Volume and Issue: 5(1), P. 64 - 82
Published: Feb. 6, 2024
Stem cell therapy is a novel approach for treating various severe and intractable diseases, including autoimmune disorders, organ transplants, tumors, neurodegenerative diseases. Nevertheless, the extensive utilization of stem cells constrained by potential tumorigenicity, challenges in precise differentiation, rejection concerns, ethical considerations. Extracellular vesicles possess ability to carry diverse bioactive factors from deliver them specific target or tissues. Moreover, they offer advantage low immunogenicity. Consequently, have facilitate therapeutic cells, mitigating risks associated with direct application. Therefore, use extracellular clinical diseases has received increasing attention. This review summarizes advances mesenchymal (MSC). MSC are used treatment inflammatory such as rheumatoid arthritis, liver injury, COVID-19, allergies; repair tissue damage heart disease, kidney osteoarthritic diseases; carrier tumors; regenerative agent disorders Alzheimer's Parkinson's.
Language: Английский
Citations
4
The Kaohsiung Journal of Medical Sciences, Journal Year: 2025, Volume and Issue: unknown
Published: March 7, 2025
The purpose of this study was to investigate the role LncRNA SNHG14 delivered by bone marrow mesenchymal stem cells-secreted exosomes (BMSC-Exos) in osteoporosis (OP). BMSCs and BMSCs-Exos were isolated identified. transfected, from which collected. treated co-cultured with BMSCs. After osteogenic induction BMSCs, calcification analyzed alizarin red S staining. adipogenic lipid droplets detected oil O Glycerol-3-phosphate dehydrogenase activity measured OVX mouse models established BMSC-EXOs. HE staining IHC performed on femurs mice. interaction between SNHG14, miR-27a-3p, LMNB1 evaluated luciferase reporter gene assay RIP assay. Gene levels assessed using RT-qPCR Western blot, respectively. BMSC-Exos promoted osteogenic-adipogenic balance enhanced promoting effect osteogenesis adipogenesis directly bound miR-27a-3p. SNHG1 mediated via a target involved process regulating overexpression formation alleviated OP vivo. regulates mediating miR-27a-3p/LMNB1 axis.
Language: Английский
Citations
0