Chinese Medical Journal,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 12, 2024
In
multiple
areas
such
as
science,
technology,
and
economic
activities,
it
is
necessary
to
unify
the
management
of
repetitive
tasks
or
concepts
by
standardization
obtain
best
order
high
efficiency.
Organoids,
living
tissue
models,
have
rapidly
developed
in
past
decade.
Organoids
can
be
used
repetitively
for
vitro
culture,
cryopreservation,
recovery
further
utilization.
Because
organoids
recapitulate
parental
tissues'
morphological
phenotypes,
cell
functions,
biological
behaviors,
genomic
profiles,
they
are
known
renewable
"living
biobanks".
cover
two
mainstream
fields:
Adult
stem
cell-derived
(also
patient-derived
organoids)
induced
pluripotent
and/or
embryonic
organoids.
Given
increasing
importance
development
new
drugs,
standardized
operation,
all
steps
organoid
construction
an
important
guarantee
ensure
quality
products.
this
review,
we
systematically
introduce
operation
procedures,
laboratory
construction,
available
documents
related
culture
that
been
published
so
far.
We
also
proposed
challenges
prospects
field.
BMC Ophthalmology,
Journal Year:
2025,
Volume and Issue:
25(1)
Published: Jan. 10, 2025
Aniridia
is
a
rare
panocular
disease
caused
by
gene
mutation
in
the
PAX6,
which
essential
for
eye
development.
inherited
an
autosomal
dominant
manner,
but
its
phenotype
can
vary
significantly
among
individuals
with
same
mutation.
Animal
models,
such
as
drosophila,
zebrafish,
and
rodents,
have
been
used
to
study
aniridia
through
Pax6
deletions.
Recently,
patient-derived
limbal
epithelial
stem
cells
(LESCs)
human-induced
pluripotent
(hiPSCs)
model
vitro,
providing
new
insights
into
therapeutic
strategies.
In
this
study,
corneal
organoids
were
generated
from
hiPSCs
derived
patients
three
different
PAX6
nonsense
mutations,
allowing
detailed
comparison
between
diseased
healthy
control
models.
These
structurally
mimicked
human
cornea
investigate
histologic
metabolomic
differences
aniridia-derived
samples.
Untargeted
analysis
revealed
significant
metabolic
wild-type
(WT)
aniridia-associated
keratopathy
(AAK)
hiPSCs.
Further
profiling
at
time
points
demonstrated
distinct
shifts,
amino
acid
metabolism
pathways
being
consistently
enriched
AAK
organoids.
This
emphasizes
profound
impact
of
mutations
on
metabolism,
particularly
biosynthesis
energy
pathways.
Turkish Journal of Ophthalmology,
Journal Year:
2024,
Volume and Issue:
unknown, P. 159 - 169
Published: June 28, 2024
Tissue
engineering
(TE)
is
a
field
of
science
that
combines
biological,
engineering,
and
medical
sciences
allows
the
development
disease
models,
drug
gene
therapy
studies,
even
cellular
or
tissue-based
treatments
developed
by
methods.The
eye
an
organ
easily
accessible
amenable
to
applications,
paving
way
for
TE
in
ophthalmology.TE
studies
are
being
conducted
on
wide
range
topics,
including
tear
film,
eyelids,
cornea,
optic
nerve,
glaucoma,
retinal
diseases.With
rapid
scientific
advances
field,
it
seems
radically
modifying
management
ocular
disorders.
Current Ophthalmology Reports,
Journal Year:
2024,
Volume and Issue:
12(4), P. 55 - 62
Published: July 5, 2024
Abstract
Purpose
of
Review
We
review
the
latest
developments
in
restoration
lacrimal
gland
function
dry
eye
disease
and
promising
methods
to
generate
functional
units.
Recent
Findings
Mesenchymal
stem
cell
delivery
demonstrated
improved
tear
secretion
mouse
models
early
human
studies,
likely
through
immune
modulation
repair
mechanisms.
Advances
regenerative
strategies
create
units
included
new
porcine
scaffolds,
organ
germ
method,
novel
organoids,
3-dimensional
bioprinting.
FGF
signaling
holds
an
important
role
development
growth
epithelium.
Summary
various
approaches
restoring
engineering
show
promise
for
future
clinical
application.
Advanced Science,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 27, 2024
Abstract
The
primary
role
of
the
parathyroid
glands
is
to
maintain
calcium
homeostasis
through
secretion
hormone
(PTH).
limited
proliferative
capacity
and
differentiation
cells
hinder
generation
cell
therapy
options.
In
this
study,
organoids
are
successfully
generated
from
human‐induced
pluripotent
stem
(hiPSCs).
At
end
20
days
differentiation,
exhibited
distinct
morphology.
Stereomicroscope,
scanning
electron
microscopy
(SEM),
transmission
(TEM)
analysis
demonstrated
3D
arrangement
layers
in
which
intracellular
structures
resemble
human
cellular
Comprehensive
molecular
analyses,
including
RNA
sequencing
(RNA‐Seq)
liquid
chromatography/mass
spectrometry
(LC‐MS/MS),
confirmed
expression
key
parathyroid‐related
markers.
Protein
CasR,
CxCr4,
Gcm2,
PTH
observed
organoids.
Parathyroid
secrete
PTH,
demonstrate
active
intercellular
signaling,
induce
osteogenic
via
their
secretome.
tissue
integration
potential
determined
by
transplantation
into
parathyroidectomized
rats.
organoid
transplanted
animals
showed
significant
elevations
PTH‐related
markers
(CasR,
Foxn1,
PTH).
detected
organoid‐transplanted
animals.
findings
represent
a
advancement
culture
may
offer
for
treating
diseases,
hypoparathyroidism.
Materials,
Journal Year:
2024,
Volume and Issue:
17(22), P. 5425 - 5425
Published: Nov. 6, 2024
The
lacrimal
gland
(LG)
is
vital
for
ocular
health,
producing
tears
that
lubricate
and
protect
the
eye.
Dysfunction
of
LG
leads
to
aqueous-deficient
dry
eye
disease
(DED),
significantly
impacting
quality
life.
Current
treatments
mainly
address
symptoms
rather
than
underlying
dysfunction,
highlighting
need
regenerative
therapies.
Tissue
engineering
offers
a
promising
solution,
with
biomaterials
playing
crucial
roles
in
scaffolding
supporting
cell
growth
regeneration.
This
review
focuses
on
recent
advances
used
tissue
gland.
We
discuss
both
natural
synthetic
mimic
extracellular
matrix
provide
structural
support
proliferation
differentiation.
Natural
biomaterials,
such
as
Matrigel,
decellularized
matrices,
chitosan,
silk
fibroin
hydrogels,
human
amniotic
membrane
are
evaluated
their
biocompatibility
ability
cells.
Synthetic
like
polyethersulfone,
polyesters,
biodegradable
polymers
(PLLA
PLGA),
assessed
mechanical
properties
potential
create
scaffolds
replicate
complex
architecture
LG.
also
explore
integration
factors
stem
cells
these
enhance
Challenges
achieving
proper
vascularization,
innervation,
long-term
functionality
engineered
tissues
discussed.
Advances
3D
bioprinting
scaffold
fabrication
techniques
highlighted
avenues
overcome
current
limitations.
