Quantitative proteomics unveils potential plasma biomarkers and provides insights into the pathophysiological mechanisms underlying equine metabolic syndrome DOI

Elisa María Espinosa-López,

Beatriz Ortiz-Guisado,

E. Díez de Castro

et al.

Published: April 7, 2025

Abstract Background Equine Metabolic Syndrome (EMS) is a multifactorial endocrine disorder characterized by obesity, insulin dysregulation (ID), and an increase in the risk of laminitis, painful condition that can lead to euthanasia severe cases. Diagnosing EMS challenging often relies on clinical history including difficulty losing weight, recurring episodes laminitis. The gold standard for laboratory support diagnosis identification ID, being basal simplest most accessible method. However, various factors such as diet, age, stress, season, testing protocols influence results. Dynamic tests like oral sugar test (OST) are preferred but present limitations due low sensitivity poor repeatability. These diagnostic challenges make difficult detect veterinary medicine highlighting need effective method early detection prevent laminitis its associated complications. Results Mass spectrometry-based proteomics represents powerful tool identify biomarkers explore molecular pathways related underlying pathology. In current study we established integrated pipeline plasma diagnosis. We compared proteomes from healthy horses, non-ID obese horses animals diagnosed with EMS. This comparison revealed 76 proteins significant changes (1% FDR) between groups. Conclusions Our demonstrates complement system, coagulation cascade extracellular matrix remodelling altered findings offer new insights into basis development led nomination several potential detection.

Language: Английский

The importance of some specific proteins in the pathogenesis and diagnosis of diabetic peripheral polyneuropathy in children DOI Creative Commons
G.O. Lezhenko,

M.S. Spilnik

CHILD`S HEALTH, Journal Year: 2025, Volume and Issue: 20(1), P. 41 - 47

Published: March 5, 2025

Background. Considering that most diagnostic tests for diabetic peripheral polyneuropathy (DPN) are not suitable use in childhood, resulting low accuracy of this complication, there is a need to identify reliable and simple markers early detection monitoring progression children. Purpose: study the content fetuin A, gamma-aminobutyric acid (GABA), S100 protein copeptin blood serum children with type 1 diabetes mellitus determine their role development polyneuropathy. Materials methods. We examined 63 aged 10 17 years. Group included 26 patients without signs neuropathy, group 2 consisted 37 The control 29 representative terms age gender carbohydrate metabolism disorders. levels acid, protein, were determined by enzyme-linked immunosorbent assay using commercial kits. Results. It has been proven who did have DPN, was 1.6-fold increase A 2.4-fold GABA compared (p < 0.05). With decrease both GABA. significant statistical level found while values statistically differ from > An severity neurological deficit inversely related (r = –0.40; p 0.05) –0.45; positively correlated 0.66; 0.68; Conclusions. comprehensive fetuin, GABA, can act as an additional objective marker DPN will allow objectification improvement diagnosis complication.

Language: Английский

Citations

0
Skeletal Changes and Vascular Calcifications in Chronic Kidney Disease: Two Connected Cups DOI Open Access

Alexander Jančuška,

Martin Kužma,

Roman Králik

et al.

Bratislavské lekárske listy/Bratislava medical journal, Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

Abstract In chronic kidney disease, calcium and phosphate imbalances occur, causing numerous dysfunctions of related regulatory mechanisms, called disease–mineral bone disorder (CKD-MBD). Its consequences include impaired health heightened risk fractures, extensive soft tissue calcification, most significantly affecting arteries, leading to the deterioration cardiovascular health. Over past decades, many pathophysiological connections have been found markers discovered that can be used evaluate status, as well extent vascular calcifications. These a multitude older newer laboratory parameters imaging methods. The use effective predictive scoring systems improve identification patients who are at developing complications. Aside from discoveries novel markers, opportunistic some commonly utilised diagnostic methods patient has already undergone for other indications powerful tool.

Language: Английский

Citations

0
Unraveling the Mystery of Insulin Resistance: From Principle Mechanistic Insights and Consequences to Therapeutic Interventions DOI Open Access
Rashid Mir, Mohammed Jeelani, Muffarah Hamid Alharthi

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2770 - 2770

Published: March 19, 2025

Insulin resistance (IR) is a significant factor in the development and progression of metabolic-related diseases like dyslipidemia, T2DM, hypertension, nonalcoholic fatty liver disease, cardiovascular cerebrovascular disorders, cancer. The pathogenesis IR depends on multiple factors, including age, genetic predisposition, obesity, oxidative stress, among others. Abnormalities insulin-signaling cascade lead to host, insulin receptor abnormalities, internal environment disturbances, metabolic alterations muscle, liver, cellular organelles. complex multifaceted characteristics signaling envisage their thorough comprehensive understanding at molecular level. Therapeutic strategies for include exercise, dietary interventions, pharmacotherapy. However, there are still gaps be addressed, more precise biomarkers associated chronic lifestyle interventions needed. Understanding these pathways essential developing effective treatments IR, reducing healthcare costs, improving quality patient life.

Language: Английский

Citations

0
New Cardiovascular Risk Biomarkers in Rheumatoid Arthritis: Implications and Clinical Utility—A Narrative Review DOI Creative Commons
Anna Pàmies, Joan-Carles Vallvé, Sílvia Paredes

et al.

Biomedicines, Journal Year: 2025, Volume and Issue: 13(4), P. 870 - 870

Published: April 3, 2025

Rheumatoid arthritis (RA) is a chronic autoimmune disease that not only causes joint inflammation but also significantly increases the risk of cardiovascular (CVD), leading to higher morbidity and mortality. RA patients face an accelerated progression atherosclerosis, attributed both traditional factors systemic inflammation. This review focuses on emerging biomarkers for assessment in RA, aiming enhance early detection treatment strategies. Specifically, we examine roles interleukin-32 (IL-32), Dickkopf-1 (DKK-1), galectin-3 (Gal-3), catestatin (CST), fetuin-A (Fet-A) as potential markers CVD this patient population. IL-32, proinflammatory cytokine, elevated plays significant role endothelial dysfunction, which contribute atherosclerosis. DKK-1, Wnt signaling pathway inhibitor, has been associated with synovial development atherosclerotic plaques. Elevated DKK-1 levels have linked increased CV mortality could serve marker RA. Gal-3 involved immune modulation fibrosis, correlating activity outcomes. Catestatin, peptide derived from chromogranin A, protective anti-inflammatory antioxidative properties, though its RA-related remains under investigation. Finally, Fet-A, glycoprotein vascular calcification, shows biomarker events data remain conflicting. These provide deeper insights into pathophysiology comorbidities. Although some show promise improving stratification, further large-scale studies are required validate their clinical utility. Currently, these research phase yet implemented standard care. Identifying incorporating routine practice lead better management patients, thus outcomes high-risk highlights importance continued establish reliable can aid diagnosis targeted therapies complications

Language: Английский

Citations

0
Quantitative proteomics unveils potential plasma biomarkers and provides insights into the pathophysiological mechanisms underlying equine metabolic syndrome DOI

Elisa María Espinosa-López,

Beatriz Ortiz-Guisado,

E. Díez de Castro

et al.

Published: April 7, 2025

Abstract Background Equine Metabolic Syndrome (EMS) is a multifactorial endocrine disorder characterized by obesity, insulin dysregulation (ID), and an increase in the risk of laminitis, painful condition that can lead to euthanasia severe cases. Diagnosing EMS challenging often relies on clinical history including difficulty losing weight, recurring episodes laminitis. The gold standard for laboratory support diagnosis identification ID, being basal simplest most accessible method. However, various factors such as diet, age, stress, season, testing protocols influence results. Dynamic tests like oral sugar test (OST) are preferred but present limitations due low sensitivity poor repeatability. These diagnostic challenges make difficult detect veterinary medicine highlighting need effective method early detection prevent laminitis its associated complications. Results Mass spectrometry-based proteomics represents powerful tool identify biomarkers explore molecular pathways related underlying pathology. In current study we established integrated pipeline plasma diagnosis. We compared proteomes from healthy horses, non-ID obese horses animals diagnosed with EMS. This comparison revealed 76 proteins significant changes (1% FDR) between groups. Conclusions Our demonstrates complement system, coagulation cascade extracellular matrix remodelling altered findings offer new insights into basis development led nomination several potential detection.

Language: Английский

Citations

0