Blood Research,
Journal Year:
2024,
Volume and Issue:
59(1)
Published: Dec. 1, 2024
Abstract
Introduction
Despite
advances
in
the
treatment
of
acute
myeloid
leukemia
(AML),
refractory
forms
this
malignancy
and
relapse
remain
common.
Therefore,
development
novel,
synergistic
targeted
therapies
are
needed
urgently.
Recently,
mesenchymal
stem
cells
(MSCs)
have
been
shown
to
be
effective
treating
various
diseases,
with
most
their
therapeutic
outcomes
attributed
exosomes.
In
current
study,
we
investigated
effects
bone
marrow
cell
(BM-MSC)
exosomes
on
expression
Janus
kinase/signal
transducers
activators
transcription
(JAK/STAT)
signaling
genes
involved
AML
pathogenesis.
Material
Methods
Exosomes
were
isolated
from
BM-MSCs
confirmed
using
transmission
electron
microscopy,
dynamic
light
scattering,
flow
cytometry.
Subsequently,
exosome
concentration
was
estimated
bicinchoninic
acid
assay,
HL-60
cocultured
100
µg/mL
BM-MSC
Finally,
JAK2,
STAT3,
STAT5
levels
analyzed
qRT-PCR.
Results
The
characterization
results
that
nanoparticles
exhibited
a
round
morphology,
expressed
CD9,
CD63,
CD81,
which
specific
protein
markers
for
identification,
ranged
between
80
nm
diameter.
Furthermore,
qRT-PCR
analysis
revealed
significant
downregulation
treated
μg/mL
Conclusion
Since
JAK/STAT
contributes
survival,
our
findings
suggest
by
leukemic
may
aid
designing
potent
strategy
treatment.
Kidney Diseases,
Journal Year:
2024,
Volume and Issue:
10(4), P. 303 - 312
Published: Jan. 1, 2024
Diabetic
kidney
disease
(DKD),
a
metabolism-related
syndrome
characterized
by
abnormal
glomerular
filtration
rate,
proteinuria,
and
renal
microangiopathy,
is
one
of
the
most
common
forms
chronic
disease,
whereas
extracellular
vesicles
(EVs)
have
been
recently
evidenced
as
novel
cell
communication
player
in
DKD
occurrence
progress
via
releasing
various
bioactive
molecules,
including
proteins,
lipids,
especially
RNA,
among
which
noncoding
RNAs
(including
miRNAs,
lncRNAs,
circRNAs)
are
major
regulators.
However,
functional
relevance
EV-derived
ncRNAs
to
be
elucidated.
Acta Materia Medica,
Journal Year:
2024,
Volume and Issue:
3(2)
Published: Jan. 1, 2024
To
determine
the
protective
effects
of
FM0807
against
diabetes-induced
renal
inflammation
and
fibrosis
underlying
mechanisms
in
vivo
vitro
.
was
administered
to
db/db
mice.
Glomerular
mesangial
cells
(HBZY-1)
were
cultured
under
high
glucose
conditions
with
or
without
FM0807.
Gene
protein
expression
assessed
by
quantitative
real-time
PCR,
western
blotting,
immunofluorescence.
Mitochondrial
reactive
oxygen
species
detected
MitoSOX
Red.
markedly
reduced
blood
glucose,
glycosylated
hemoglobin,
triglycerides,
low-density
lipoprotein-cholesterol
levels
improved
liver
organ
index,
high-density
level,
function,
as
evidenced
decreased
24-h
urinary
excretion
creatinine
urea
nitrogen
levels.
ameliorated
pathologic
changes
diabetic
mice
(reduced
glomerulosclerosis,
diminished
interstitial
cellular
inflammation,
less
tubular
luminal
narrowing).
Treatment
also
led
a
significant
reduction
inflammatory
markers,
including
JAK2,
STAT3,
TNF-α,
IL-1β,
IL-6,
TGF-β1,
Smad2/3,
addition
alterations
proteins
associated
kidney
injury.
These
data
suggest
that
alleviates
modulating
JAK2/STAT3
TGF-β1/SMAD2/3
signaling
pathways.
Biomedicines,
Journal Year:
2024,
Volume and Issue:
12(11), P. 2608 - 2608
Published: Nov. 14, 2024
Type
2
diabetes
mellitus
(T2DM)
is
a
metabolic
disorder,
and
urinary
exosomal
microRNAs
(miRNAs)
were
utilized
as
potential
disease
prediction
or
diagnostic
biomarkers
in
numerous
studies.
This
study
investigated
the
differential
expression
of
miRNAs
between
non-diabetes
(NDM)
individuals
those
with
T2DM.
Blood Research,
Journal Year:
2024,
Volume and Issue:
59(1)
Published: Dec. 1, 2024
Abstract
Introduction
Despite
advances
in
the
treatment
of
acute
myeloid
leukemia
(AML),
refractory
forms
this
malignancy
and
relapse
remain
common.
Therefore,
development
novel,
synergistic
targeted
therapies
are
needed
urgently.
Recently,
mesenchymal
stem
cells
(MSCs)
have
been
shown
to
be
effective
treating
various
diseases,
with
most
their
therapeutic
outcomes
attributed
exosomes.
In
current
study,
we
investigated
effects
bone
marrow
cell
(BM-MSC)
exosomes
on
expression
Janus
kinase/signal
transducers
activators
transcription
(JAK/STAT)
signaling
genes
involved
AML
pathogenesis.
Material
Methods
Exosomes
were
isolated
from
BM-MSCs
confirmed
using
transmission
electron
microscopy,
dynamic
light
scattering,
flow
cytometry.
Subsequently,
exosome
concentration
was
estimated
bicinchoninic
acid
assay,
HL-60
cocultured
100
µg/mL
BM-MSC
Finally,
JAK2,
STAT3,
STAT5
levels
analyzed
qRT-PCR.
Results
The
characterization
results
that
nanoparticles
exhibited
a
round
morphology,
expressed
CD9,
CD63,
CD81,
which
specific
protein
markers
for
identification,
ranged
between
80
nm
diameter.
Furthermore,
qRT-PCR
analysis
revealed
significant
downregulation
treated
μg/mL
Conclusion
Since
JAK/STAT
contributes
survival,
our
findings
suggest
by
leukemic
may
aid
designing
potent
strategy
treatment.
