Review of Spider Silk Applications in Biomedical and Tissue Engineering

Biomimetics, Journal Year: 2024, Volume and Issue: 9(3), P. 169 - 169

Published: March 11, 2024

This review will present the latest research related to production and application of spider silk silk-based materials in reconstructive regenerative medicine tissue engineering, with a focus on musculoskeletal tissues, including skin regeneration repair bone cartilage, ligaments, muscle tissue, peripheral nerves, artificial blood vessels. Natural synthesis is reviewed, further recombinant proteins. Research insights into possible structures, like fibers (1D), coatings (2D), 3D constructs, porous hydrogels, organ-on-chip designs, have been reviewed considering design bioactive for smart medical implants drug delivery systems. Silk one toughest natural materials, high strain at failure mechanical strength. Novel biomaterials fibroin can mimic structure promote new growth. proteins are important designing tissue-on-chip or technologies micro devices precise engineering tissues organs, disease modeling, selection adequate treatments. Recent indicates that (films, capsules, liposomes coated proteins) has potential provide controlled release target destination. However, even clear advantages, there still challenges need research, clinical trials.

Language: Английский

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MicroRNA-targeting nanomedicines for the treatment of intervertebral disc degeneration

Advanced Drug Delivery Reviews, Journal Year: 2024, Volume and Issue: 207, P. 115214 - 115214

Published: Feb. 22, 2024

Language: Английский

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Development and Applications of PLGA Hydrogels for Sustained Delivery of Therapeutic Agents

Gels, Journal Year: 2024, Volume and Issue: 10(8), P. 497 - 497

Published: July 26, 2024

Poly(lactic-co-glycolic acid) (PLGA) hydrogels are highly utilized in biomedical research due to their biocompatibility, biodegradability, and other versatile properties. This review comprehensively explores synthesis, properties, sustained release mechanisms, applications drug delivery. The introduction underscores the significance of PLGA addressing challenges like short half-lives systemic toxicity conventional formulations. Synthesis methods, including emulsion solvent evaporation, casting, electrospinning, thermal gelation, photopolymerization, described detail role tailoring hydrogel properties for specific is highlighted. Sustained mechanisms—such as diffusion-controlled, degradation-controlled, swelling-controlled, combined systems—are analyzed alongside key kinetic models (zero-order, first-order, Higuchi, Peppas models) designing controlled delivery systems. Applications discussed, highlighting effectiveness localized chemotherapy cancer, well antibiotics antimicrobials combat infections. Challenges future prospects with a focus on improving loading efficiency, control promoting clinical translation. In summary, provide promising platform therapeutic agents meet diverse requirements. Future advancements materials science engineering anticipated further optimize efficacy applicability settings. consolidates current understanding outlines directions hydrogels, emphasizing potential revolutionize improve patient outcomes.

Language: Английский

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Cutting-Edge Biomaterials in Intervertebral Disc Degeneration Tissue Engineering

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(8), P. 979 - 979

Published: July 24, 2024

Intervertebral disc degeneration (IVDD) stands as the foremost contributor to low back pain (LBP), imposing a substantial weight on world economy. Traditional treatment modalities encompass both conservative approaches and surgical interventions; however, former falls short in halting IVDD progression, while latter carries inherent risks. Hence, quest for an efficacious method reverse onset is paramount. Biomaterial delivery systems, exemplified by hydrogels, microspheres, microneedles, renowned their exceptional biocompatibility, biodegradability, biological efficacy, mechanical attributes, have found widespread application bone, cartilage, various tissue engineering endeavors. Consequently, IVD has emerged burgeoning field of interest. This paper succinctly introduces intervertebral (IVD) structure pathophysiology IVDD, meticulously classifies biomaterials repair, reviews recent advances field. Particularly, strengths weaknesses are emphasized, potential avenues future research suggested.

Language: Английский

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The MnO2/GelMA Composite Hydrogels Improve the ROS Microenvironment of Annulus Fibrosus Cells by Promoting the Antioxidant and Autophagy through the SIRT1/NRF2 Pathway

Gels, Journal Year: 2024, Volume and Issue: 10(5), P. 333 - 333

Published: May 15, 2024

Intervertebral disc degeneration (IVDD) is a worldwide disease that causes low back pain and reduces quality of life. Biotherapeutic strategies based on tissue engineering alternatives, such as intervertebral scaffolds, supplemented by drug-targeted therapy have brought new hope for IVDD. In this study, to explore the role mechanism MnO2/GelMA composite hydrogels in alleviating IVDD, we prepared with MnO2 methacrylate gelatin (GelMA) characterized them using compression testing transmission electron microscopy (TEM). Annulus fibrosus cells (AFCs) were cultured verify biocompatibility live/dead cytoskeleton staining. Cell viability assays reactive oxygen species (ROS) probe used analyze protective effect under oxidative damage. To improving microenvironment, detected expression levels antioxidant autophagy-related genes proteins qPCR Western blotting. We found exhibited excellent porous structure, which promoted cell proliferation. The addition nanoparticles GelMA cleared ROS AFCs induced cellular autophagy through common SIRT1/NRF2 pathway. Therefore, hydrogels, can improve microenvironment scavenging intracellular resisting damage, great application prospects treatment

Language: Английский

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Application of an Injectable Thermosensitive Hydrogel Drug Delivery System for Degenerated Intervertebral Disc Regeneration

Biomacromolecules, Journal Year: 2024, Volume and Issue: 26(1), P. 209 - 221

Published: Dec. 13, 2024

Intervertebral disc degeneration is characterized by a localized, chronic inflammatory response leading to synthesis/catabolism imbalance within the nucleus pulposus (NP) and progressive functional impairment NP. Polyphenol molecules have been widely used in anti-inflammatory therapies recent years; therefore, we designed an injectable, temperature-sensitive hydrogel PLGA-PEG-PLGA-based drug delivery system for local sustained of two drugs tannic acid (TA) resveratrol (Res), with carrying TA directly Res indirectly (carried inflammation-responsive nanoparticles). The presents good injectability at room temperature forms gel situ upon entering intervertebral disc. can rapidly release sustain release. In vitro vivo experiments shown that this effective anti-inflammation degenerated discs promotes regeneration extracellular matrix

Language: Английский

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The Artificial Disc Nucleus and Other Strategies for Replacement of the Nucleus Pulposus: Past, Present and Future Designs for an Emerging Surgical Solution

Engineered Regeneration, Journal Year: 2024, Volume and Issue: 5(2), P. 269 - 281

Published: May 8, 2024

Nucleus Pulposus (NP) Replacement is a developing surgical methodology for the treatment of pathology related to degeneration intervertebral discs (IVDs). This article provides necessary context regarding pathologies treated with this technology, biomechanical structure and function IVD, procedures technology aims replace. Primarily, it an overview discussion commercial experimental preformed in situ curing prosthesis designs reported scientific literature summarizes results clinical studies evaluating their efficacy. Contextual updated information on most recent research into NP replacement novel hydrogel tissue engineering (TE) strategies described. allows potential improvement degenerative spinal through minimally invasive techniques.

Language: Английский

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Anterior percutaneous full-endoscopic transcorporeal decompression for cervical disc herniation: a finite element analysis and long-term follow-up study

BMC Musculoskeletal Disorders, Journal Year: 2024, Volume and Issue: 25(1)

Published: Aug. 13, 2024

The purpose of this study was to investigate the long-term consequences on cervical spine after Anterior transcorporeal percutaneous endoscopy discectomy (ATc-PECD) from biomechanical standpoint. A three-dimensional model normal C2-T1 established using finite element method. Subsequently, a disc degeneration and with surgery were constructed basis model. same loading conditions applied simulate flexion, extension, lateral bending axial rotation spine. We calculated range motion (ROM), intradiscal pressure, intravertebral body pressure under different motions for observing changes in biomechanics surgery. At time, we combined results follow-up ATc-PECD, used imaging methods measure vertebral height mobility, Japanese Orthopaedic Association (JOA) score visual analog scale (VAS) assess pain relief neurological functional recovery. revealed that preoperative JOA score, neck VAS hand IDH, VBH, ROM patients 9.49 ± 2.16, 6.34 1.68, 5.14 1.48, 5.95 0.22 mm, 15.41 1.68 52.46 9.36° respectively. It changed 15.71 1.13 (P < 0.05), 1.02 0.82 0.77 0.76 4.73 0.26 mm 13.67 1.48 59.26 6.72° respectively, at 6 years postoperatively. Finite analysis showed establishing spondylosis model, overall bending, decreased by 3.298°, 0.753°, 3.852°, 1.131° Conversely, bone tunnel these actions increased 0.843°, 0.65°, 0.278°, 0.488° consistent results. Moreover, segmental mobility primarily contributed surgical segments. Additionally, demonstrated tunneling could lead stress within bodies intervertebral discs Long-term studies have shown ATc-PECD has good clinical efficacy can be as complementary method CDH treatment. FEM internal stresses operated segments, which is directly related ATc-PECD.

Language: Английский

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Nanocomposite Hydrogels: A Promising Approach for the Treatment of Degenerative Joint Diseases

Small Science, Journal Year: 2024, Volume and Issue: 4(11)

Published: Sept. 3, 2024

Degenerative joint diseases, as a global public health issue, impose significant burdens on patients' lives and substantial economic costs society. Currently, the primary modalities include physical therapy, pharmaceutical intervention, surgical procedures. None of these approaches can alter course this degenerative process. Due to their commendable biocompatibility, biodegradability, heightened efficacy in drug delivery, hydrogels present themselves novel noninvasive remedy for ailments. However, clinical application still faces some challenges, including uncontrolled discharge encapsulated medications, absence adequate mechanical reinforcement destabilized joints, adaptability fluctuating microenvironments. Recently, nanocomposite hydrogels, formed by introducing nanomaterials into or chemical means, improve limitations extend potential biological applications diseases. In study, pathologic features diseases multiple different types targeting are briefly described. It also concludes with an outlook use settings discusses challenges limitations.

Language: Английский

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Regenerative Outcomes of Combining siCOL1A2 Hydrogel with Acupuncture in a Rat Model of Chronic Intervertebral Disc Degeneration

Bioengineering, Journal Year: 2024, Volume and Issue: 11(11), P. 1066 - 1066

Published: Oct. 25, 2024

Intervertebral disc degeneration (IVDD) is a significant cause of chronic pain and disability, necessitating innovative therapeutic strategies. This study investigates the combined effect novel siCOL1A2-encapsulated hydrogel acupuncture on IVDD in rat model. We developed system, G5-PBA (siCOL1A2@G5-PBA@Gel), designed for sustained siRNA delivery to degenerated discs assessed its efficacy alongside treatment. Key inflammatory genes were identified through RNA-seq analysis, with COL1A2 highlighted as crucial regulator responses IVDD. Our vivo experiments involved treating rats alone, combining both. The treatments evaluated behavioral assessments, imaging techniques (X-ray MRI), histological analyses. Results indicated that combination therapy significantly alleviated pain, reduced inflammation, promoted regeneration more effectively than individual treatments. proved biocompatible facilitated targeted gene silencing, while enhanced outcomes by improving local blood circulation modulating responses. These findings suggest integrating siCOL1A2 offers promising approach

Language: Английский

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