Prevalence of antibiotic-resistant Gram-negative bacteria having extended-spectrum β-lactamase phenotypes in polluted irrigation-purpose wastewaters from Indian agro-ecosystems DOI Creative Commons

Achhada Ujalkaur Avatsingh,

Sanjay Sharma,

Shilippreet Kour

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Aug. 7, 2023

Antibiotic resistance in bacteria has emerged as a serious public health threat worldwide. Aquatic environments including irrigation-purpose wastewaters facilitate the emergence and transmission of antibiotic-resistant antibiotic genes leading to detrimental effects on human environment sustainability. Considering paramount ever-increasing health, there is an urgent need for continuous environmental monitoring wastewater being used irrigation Indian agro-ecosystems. In this study, prevalence Gram-negative isolated from samples Sirmaur Solan districts Himachal Pradesh was determined. Bacterial isolates genera Escherichia, Enterobacter, Hafnia, Shigella, Citrobacter, Klebsiella obtained 11 different geographical locations were found exhibit against ampicillin, amoxyclav, cefotaxime, co-trimoxazole, tobramycin, cefpodoxime ceftazidime. However, all sensitive aminoglycoside gentamicin. Enterobacter spp. Escherichia coli showed predominance among isolates. Multidrug-resistance phenotype observed with isolate AUK-06 (Enterobacter sp.) which exhibited resistant five antibiotics. Isolate AUK-02 AUK-09, both E. strains phenotypes four antibiotics each. Phenotypic detection revealed that six positive extended-spectrum β-lactamases includes two each one Shigella sp. Citrobacter Overall, findings occurrence ESBL-positive bacterial utilized purpose study area necessitate precautionary interventions. The outcomes would be significant clinical, epidemiological, agro-environmental importance designing effective management pollution control strategies.

Language: Английский

Mapping bacterial diversity and antibiotic resistance across wastewater treatment plant stages: Insights from high-resolution 16S rRNA sequencing of the V3-V4 regions to detection of multi-drug resistant bacteria DOI

Nuona Li,

Minghua Li, Pu Chen

et al.

Journal of Water Process Engineering, Journal Year: 2025, Volume and Issue: 71, P. 107143 - 107143

Published: Feb. 9, 2025

Language: Английский

Citations

1
Detection and quantification of adenovirus, polyomavirus, and papillomavirus in urban sewage DOI Creative Commons

Mohamed N. F. Shaheen,

Nehal I. Ahmed,

Kareem Rady Badr

et al.

Journal of Water and Health, Journal Year: 2024, Volume and Issue: 22(2), P. 401 - 413

Published: Feb. 1, 2024

The objective of this study was to assess the occurrence and seasonal frequency human adenovirus (HAdV), polyomavirus (HPyV), papillomavirus (HPV) in urban sewage. detection these viruses carried out by polymerase chain reaction (PCR), then viral concentrations positive samples were quantified quantitative PCR (qPCR). Additionally, HAdV HPyV genotyping also performed PCR. A total 38/60 (63.3%) found. most prevalent virus (26/60; 43.3%), followed (21/60; 35%) HPV 35%). ranged from 3.56 × 10

Language: Английский

Citations

7
Integron-associated genes are reliable indicators of antibiotic resistance in wastewater despite treatment- and seasonality-driven fluctuations DOI Creative Commons
Rafael D.S. Tavares, Cátia Fidalgo, Elsa T. Rodrigues

et al.

Water Research, Journal Year: 2024, Volume and Issue: 258, P. 121784 - 121784

Published: May 14, 2024

The present study aims to characterize the bacterial community, resistome and integron abundance of a municipal wastewater treatment plant (WWTP) over course 12 months evaluate year-long performance integron-related genes as potential indicators antibiotic resistance mechanisms in influents effluents. For that, total DNA was extracted subjected 16S rRNA-targeted metabarcoding, high-throughput (HT) qPCR (48 targets) standard (5 targets). Targets included integrase genes, (ARGs) putative pathogenic groups. A 16 physicochemical parameters determined samples were also considered. Results revealed that WWTP significantly impacted well content ARGs genes. Indeed, there relative enrichment from influent effluent 13 groups (e.g., Legionella Mycobacterium) conferring sulphonamides, aminoglycosides disinfectants. Effluent (n = 25) presented seasonal differences, with an increase ARGs' concentration summer, differences between winter summer on sulphonamide disinfectant mechanisms. From eight selected, all positively correlated most specific classes. intI1, bla

Language: Английский

Citations

7
Primers and probes for the diagnostics of mpox virus DOI
Xingwen Chen,

Jiaoyang Xu,

Scout R. Vanderwal

et al.

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 227 - 244

Published: Jan. 1, 2025

Language: Английский

Citations

0
Synthesis and Characterization of Chitosan/Zinc Oxide Nanocomposite for Enhanced Applications as Antibacterial, Antifungal and Aflatoxin B1 Adsorption DOI
Muhammad Asif Asghar,

Farman Ahmed,

Abdur Rehman Qamar

et al.

Journal of Cluster Science, Journal Year: 2025, Volume and Issue: 36(2)

Published: Feb. 2, 2025

Language: Английский

Citations

0
Making waves: Integrating wastewater surveillance with dynamic modeling to track and predict viral outbreaks DOI Creative Commons
Tin Phan, Samantha Brozak, Bruce Pell

et al.

Water Research, Journal Year: 2023, Volume and Issue: 243, P. 120372 - 120372

Published: July 16, 2023

Language: Английский

Citations

8
RBD amplicon sequencing of wastewater reveals patterns of variant emergence and evolution DOI Creative Commons
Xingwen Chen,

John Balliew,

Cici Bauer

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 12, 2024

Abstract Rapid evolution of SARS-CoV-2 has resulted in the emergence numerous variants, posing significant challenges to public health surveillance. Clinical genome sequencing, while valuable, limitations capturing full epidemiological dynamics circulating variants general population. This study utilized receptor-binding domain (RBD) amplicon sequencing wastewater samples monitor community and El Paso, TX. Over 17 months, we identified 91 observed waves dominant transitioning from BA.2 BA.2.12.1, BA.4&5, BQ.1, XBB.1.5. Our findings demonstrated early detection identification unreported outbreaks, showing strong consistency with clinical data at local, state, national levels. Alpha diversity analyses revealed periodical variations, highest winter outbreak lag phases, likely due lower competition among before growth phase. The underscores importance low transmission periods for rapid mutation variant evolution. highlights effectiveness integrating RBD surveillance tracking viral evolution, understanding emergence, enhancing preparedness.

Language: Английский

Citations

2
Advancing Public Health Surveillance: Integrating Modeling and GIS in the Wastewater-Based Epidemiology of Viruses, a Narrative Review DOI Creative Commons
Diego F. Cuadros, Xi Chen, Jingjing Li

et al.

Pathogens, Journal Year: 2024, Volume and Issue: 13(8), P. 685 - 685

Published: Aug. 14, 2024

This review article will present a comprehensive examination of the use modeling, spatial analysis, and geographic information systems (GIS) in surveillance viruses wastewater. With advent global health challenges like COVID-19 pandemic, wastewater has emerged as crucial tool for early detection management viral outbreaks. explore application various modeling techniques that enable prediction understanding virus concentrations spread patterns systems. It highlights role analysis mapping distribution loads, providing insights into dynamics transmission within communities. The integration GIS be explored, emphasizing utility such visualizing data, enhancing sampling site selection, ensuring equitable monitoring across diverse populations. also discuss innovative combination with remote sensing data predictive offering multi-faceted approach to understand spread. Challenges quality, privacy concerns, necessity interdisciplinary collaboration addressed. concludes by underscoring transformative potential these analytical tools public health, advocating continued research innovation strengthen preparedness response strategies future threats. aims provide foundational researchers officials, fostering advancements field wastewater-based epidemiology.

Language: Английский

Citations

2
Early warnings and slow deaths: A sociology of outbreak and overdose DOI Creative Commons
Tim Rhodes, Kari Lancaster

International Journal of Drug Policy, Journal Year: 2023, Volume and Issue: 117, P. 104065 - 104065

Published: May 23, 2023

In this paper, we offer a sociological analysis of early warning and outbreak in the field drug policy, focusing on opioid overdose. We trace how 'outbreak' is enacted as rupturing event which enables rapid reflex responses precautionary control, based largely short-term proximal indicators. make case for an alternative view outbreak. argue that practices detection projection help to materialise drug-related outbreaks are too focused short-term. Engaging with epidemiological work investigating epidemics overdose, show short-termism response fails appreciate slow violent pasts indicative ongoing need care structural societal change. Accordingly, gather together ideas 'slow emergency' (Ben Anderson), death' (Lauren Berlant) violence' (Rob Nixon), re-assemble 'long view'. This locates overdose long-term attritional processes deindustrialisation, pharmaceuticalisation, other forms violence, including criminalisation problematisation people who use drugs. Outbreaks evolve relation their pasts. To ignore can perpetuate harm. Attending social conditions create possibilities invites goes 'beyond outbreak' epidemic' generally configured.

Language: Английский

Citations

6
Title: Qualitative Assessment of a Novel Results-Based Partnership between National Wastewater Surveillance Centers of Excellence and Utility Companies, Houston (Texas), Colorado, Wisconsin, and California, 2023. DOI Creative Commons
Hannah Turner, Libby Horter, Michael Welton

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 21, 2024

Abstract Background The U.S. Centers for Disease Control and Prevention (CDC) initiated the National Wastewater Surveillance System (NWSS) in September 2020. Four of Excellence (COEs) were established between 2021–2023 Houston (Texas), Colorado, Wisconsin, California to guide wastewater surveillance efforts public health. Our objective was increase understanding factors that facilitated implementation from perspectives experiences shared by health department COEs utility partners. Methods We used a purposive sampling strategy select eight key respondents four NWSS respective conducted in-depth interviews related identified common points interview transcription files. Insights on WWS partners distilled responses into lessons learned. Results Three primary themes emerged after we analyzed responses: perceived community benefits surveillance, collaboration trust building among helped program advancement, sustainability strategies considerations. Conclusion This analysis has highlighted importance partnerships collaborations.

Language: Английский

Citations

1