Prevalence of antibiotic-resistant Gram-negative bacteria having extended-spectrum β-lactamase phenotypes in polluted irrigation-purpose wastewaters from Indian agro-ecosystems DOI Creative Commons

Achhada Ujalkaur Avatsingh,

Sanjay Sharma,

Shilippreet Kour

и другие.

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Авг. 7, 2023

Antibiotic resistance in bacteria has emerged as a serious public health threat worldwide. Aquatic environments including irrigation-purpose wastewaters facilitate the emergence and transmission of antibiotic-resistant antibiotic genes leading to detrimental effects on human environment sustainability. Considering paramount ever-increasing health, there is an urgent need for continuous environmental monitoring wastewater being used irrigation Indian agro-ecosystems. In this study, prevalence Gram-negative isolated from samples Sirmaur Solan districts Himachal Pradesh was determined. Bacterial isolates genera Escherichia, Enterobacter, Hafnia, Shigella, Citrobacter, Klebsiella obtained 11 different geographical locations were found exhibit against ampicillin, amoxyclav, cefotaxime, co-trimoxazole, tobramycin, cefpodoxime ceftazidime. However, all sensitive aminoglycoside gentamicin. Enterobacter spp. Escherichia coli showed predominance among isolates. Multidrug-resistance phenotype observed with isolate AUK-06 (Enterobacter sp.) which exhibited resistant five antibiotics. Isolate AUK-02 AUK-09, both E. strains phenotypes four antibiotics each. Phenotypic detection revealed that six positive extended-spectrum β-lactamases includes two each one Shigella sp. Citrobacter Overall, findings occurrence ESBL-positive bacterial utilized purpose study area necessitate precautionary interventions. The outcomes would be significant clinical, epidemiological, agro-environmental importance designing effective management pollution control strategies.

Язык: Английский

Mapping bacterial diversity and antibiotic resistance across wastewater treatment plant stages: Insights from high-resolution 16S rRNA sequencing of the V3-V4 regions to detection of multi-drug resistant bacteria DOI

Nuona Li,

Minghua Li, Pu Chen

и другие.

Journal of Water Process Engineering, Год журнала: 2025, Номер 71, С. 107143 - 107143

Опубликована: Фев. 9, 2025

Язык: Английский

Процитировано

1
Detection and quantification of adenovirus, polyomavirus, and papillomavirus in urban sewage DOI Creative Commons

Mohamed N. F. Shaheen,

Nehal I. Ahmed,

Kareem Rady Badr

и другие.

Journal of Water and Health, Год журнала: 2024, Номер 22(2), С. 401 - 413

Опубликована: Фев. 1, 2024

The objective of this study was to assess the occurrence and seasonal frequency human adenovirus (HAdV), polyomavirus (HPyV), papillomavirus (HPV) in urban sewage. detection these viruses carried out by polymerase chain reaction (PCR), then viral concentrations positive samples were quantified quantitative PCR (qPCR). Additionally, HAdV HPyV genotyping also performed PCR. A total 38/60 (63.3%) found. most prevalent virus (26/60; 43.3%), followed (21/60; 35%) HPV 35%). ranged from 3.56 × 10

Язык: Английский

Процитировано

7
Integron-associated genes are reliable indicators of antibiotic resistance in wastewater despite treatment- and seasonality-driven fluctuations DOI Creative Commons
Rafael D.S. Tavares, Cátia Fidalgo, Elsa T. Rodrigues

и другие.

Water Research, Год журнала: 2024, Номер 258, С. 121784 - 121784

Опубликована: Май 14, 2024

The present study aims to characterize the bacterial community, resistome and integron abundance of a municipal wastewater treatment plant (WWTP) over course 12 months evaluate year-long performance integron-related genes as potential indicators antibiotic resistance mechanisms in influents effluents. For that, total DNA was extracted subjected 16S rRNA-targeted metabarcoding, high-throughput (HT) qPCR (48 targets) standard (5 targets). Targets included integrase genes, (ARGs) putative pathogenic groups. A 16 physicochemical parameters determined samples were also considered. Results revealed that WWTP significantly impacted well content ARGs genes. Indeed, there relative enrichment from influent effluent 13 groups (e.g., Legionella Mycobacterium) conferring sulphonamides, aminoglycosides disinfectants. Effluent (n = 25) presented seasonal differences, with an increase ARGs' concentration summer, differences between winter summer on sulphonamide disinfectant mechanisms. From eight selected, all positively correlated most specific classes. intI1, bla

Язык: Английский

Процитировано

7
Primers and probes for the diagnostics of mpox virus DOI
Xingwen Chen,

Jiaoyang Xu,

Scout R. Vanderwal

и другие.

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 227 - 244

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Synthesis and Characterization of Chitosan/Zinc Oxide Nanocomposite for Enhanced Applications as Antibacterial, Antifungal and Aflatoxin B1 Adsorption DOI
Muhammad Asif Asghar,

Farman Ahmed,

Abdur Rehman Qamar

и другие.

Journal of Cluster Science, Год журнала: 2025, Номер 36(2)

Опубликована: Фев. 2, 2025

Язык: Английский

Процитировано

0
Making waves: Integrating wastewater surveillance with dynamic modeling to track and predict viral outbreaks DOI Creative Commons
Tin Phan, Samantha Brozak, Bruce Pell

и другие.

Water Research, Год журнала: 2023, Номер 243, С. 120372 - 120372

Опубликована: Июль 16, 2023

Язык: Английский

Процитировано

8
RBD amplicon sequencing of wastewater reveals patterns of variant emergence and evolution DOI Creative Commons
Xingwen Chen,

John Balliew,

Cici Bauer

и другие.

medRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июль 12, 2024

Abstract Rapid evolution of SARS-CoV-2 has resulted in the emergence numerous variants, posing significant challenges to public health surveillance. Clinical genome sequencing, while valuable, limitations capturing full epidemiological dynamics circulating variants general population. This study utilized receptor-binding domain (RBD) amplicon sequencing wastewater samples monitor community and El Paso, TX. Over 17 months, we identified 91 observed waves dominant transitioning from BA.2 BA.2.12.1, BA.4&5, BQ.1, XBB.1.5. Our findings demonstrated early detection identification unreported outbreaks, showing strong consistency with clinical data at local, state, national levels. Alpha diversity analyses revealed periodical variations, highest winter outbreak lag phases, likely due lower competition among before growth phase. The underscores importance low transmission periods for rapid mutation variant evolution. highlights effectiveness integrating RBD surveillance tracking viral evolution, understanding emergence, enhancing preparedness.

Язык: Английский

Процитировано

2
Advancing Public Health Surveillance: Integrating Modeling and GIS in the Wastewater-Based Epidemiology of Viruses, a Narrative Review DOI Creative Commons
Diego F. Cuadros, Xi Chen, Jingjing Li

и другие.

Pathogens, Год журнала: 2024, Номер 13(8), С. 685 - 685

Опубликована: Авг. 14, 2024

This review article will present a comprehensive examination of the use modeling, spatial analysis, and geographic information systems (GIS) in surveillance viruses wastewater. With advent global health challenges like COVID-19 pandemic, wastewater has emerged as crucial tool for early detection management viral outbreaks. explore application various modeling techniques that enable prediction understanding virus concentrations spread patterns systems. It highlights role analysis mapping distribution loads, providing insights into dynamics transmission within communities. The integration GIS be explored, emphasizing utility such visualizing data, enhancing sampling site selection, ensuring equitable monitoring across diverse populations. also discuss innovative combination with remote sensing data predictive offering multi-faceted approach to understand spread. Challenges quality, privacy concerns, necessity interdisciplinary collaboration addressed. concludes by underscoring transformative potential these analytical tools public health, advocating continued research innovation strengthen preparedness response strategies future threats. aims provide foundational researchers officials, fostering advancements field wastewater-based epidemiology.

Язык: Английский

Процитировано

2
Early warnings and slow deaths: A sociology of outbreak and overdose DOI Creative Commons
Tim Rhodes, Kari Lancaster

International Journal of Drug Policy, Год журнала: 2023, Номер 117, С. 104065 - 104065

Опубликована: Май 23, 2023

In this paper, we offer a sociological analysis of early warning and outbreak in the field drug policy, focusing on opioid overdose. We trace how 'outbreak' is enacted as rupturing event which enables rapid reflex responses precautionary control, based largely short-term proximal indicators. make case for an alternative view outbreak. argue that practices detection projection help to materialise drug-related outbreaks are too focused short-term. Engaging with epidemiological work investigating epidemics overdose, show short-termism response fails appreciate slow violent pasts indicative ongoing need care structural societal change. Accordingly, gather together ideas 'slow emergency' (Ben Anderson), death' (Lauren Berlant) violence' (Rob Nixon), re-assemble 'long view'. This locates overdose long-term attritional processes deindustrialisation, pharmaceuticalisation, other forms violence, including criminalisation problematisation people who use drugs. Outbreaks evolve relation their pasts. To ignore can perpetuate harm. Attending social conditions create possibilities invites goes 'beyond outbreak' epidemic' generally configured.

Язык: Английский

Процитировано

6
Title: Qualitative Assessment of a Novel Results-Based Partnership between National Wastewater Surveillance Centers of Excellence and Utility Companies, Houston (Texas), Colorado, Wisconsin, and California, 2023. DOI Creative Commons
Hannah Turner, Libby Horter, Michael Welton

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Авг. 21, 2024

Abstract Background The U.S. Centers for Disease Control and Prevention (CDC) initiated the National Wastewater Surveillance System (NWSS) in September 2020. Four of Excellence (COEs) were established between 2021–2023 Houston (Texas), Colorado, Wisconsin, California to guide wastewater surveillance efforts public health. Our objective was increase understanding factors that facilitated implementation from perspectives experiences shared by health department COEs utility partners. Methods We used a purposive sampling strategy select eight key respondents four NWSS respective conducted in-depth interviews related identified common points interview transcription files. Insights on WWS partners distilled responses into lessons learned. Results Three primary themes emerged after we analyzed responses: perceived community benefits surveillance, collaboration trust building among helped program advancement, sustainability strategies considerations. Conclusion This analysis has highlighted importance partnerships collaborations.

Язык: Английский

Процитировано

1