Improvement of Low-Cost Commercial Carbon Screen-Printed Electrodes Conductivities with Controlled Gold Reduction Towards Thiol Modification DOI

Kristin Partanen,

Dianne Lee,

Adekunle Omoboye

et al.

Journal of The Electrochemical Society, Journal Year: 2023, Volume and Issue: 170(9), P. 092510 - 092510

Published: Sept. 1, 2023

Effectively detecting bacteria in the environment is crucial for researchers to make informed decisions about safety of public areas, such as lakes. This led an increased need development portable handheld devices, capable on-the-spot chemical and biological sensing applications. Specific interests lie electrochemical biosensors screen-printed electrodes (SPEs) due decreased costs, ability integrate with their user-friendly nature. Together, these qualities devices more accessible resource-poor settings. Two most common substrates used fabricate SPEs are carbon gold. Carbon effective applications yet challenged when attempting covalently attach biomolecules surface. Gold have higher affinity towards improve sensitivity, selectivity, stability a device; they can be costly. A SPE modified gold may ideal candidate create efficient low-cost device, using deposition. In this study, deposition on explored enhance surface area conductivity These were then thiol-based self-assembled monolayer (SAM) which demonstrates technique could further modification biosensing.

Language: Английский

NFC-enabled potentiostat and nitrocellulose-based metal electrodes for electrochemical lateral flow assay DOI Creative Commons
Laura Gonzalez‐Macia, Y. H. Li, Kaijia Zhang

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 251, P. 116124 - 116124

Published: Feb. 12, 2024

Rapid detection of pathogens at the point-of-need is crucial for preventing spread human, animal and plant diseases which can have devastating consequences both on lives livelihood billions people. Colorimetric, lateral flow assays consisting a nitrocellulose membrane, are preferred format today low-cost on-site pathogens. This assay has, however, historically suffered from poor analytical performance not compatible with digital technologies. In this work, we report development new class diagnostics platform precision testing. versatile consists two important innovations: i) A wireless batteryless, microcontroller-based, Near Field Communication (NFC)-enabled potentiostat that brings high electroanalytical techniques (cyclic voltammetry, chronoamperometry, square wave voltammetry) to field. The NFC-potentiostat be operated mobile app by minimally trained users; ii) approach producing membranes integrated electrodes facilitate electrochemical point-of-need. We produced an system housed in 3D-printed phone case demonstrated its use Maize Mosaic Virus (MMV), pathogen, as proof-of-concept application.

Language: Английский

Citations

8
Boron-Doped Diamond Electrode Outperforms the State-of-the-Art Electrochemiluminescence from Microbeads Immunoassay DOI

Kohei Sakanoue,

Andrea Fiorani, Claudio Santo

et al.

ACS Sensors, Journal Year: 2022, Volume and Issue: 7(4), P. 1145 - 1155

Published: March 17, 2022

Electrochemiluminescence (ECL) is a powerful transduction technique where light emission from molecular species triggered by an electrochemical reaction. Application to biosensors has led wide range of electroanalytical methods with particular impact on clinical analysis for diagnostic and therapeutic monitoring. Therefore, the quest increasing sensitivity while maintaining reproducible easy procedures brought investigations innovations in (i) electrode materials, (ii) luminophores, (iii) reagents. Particularly, ECL signal strongly affected material its surface modification during experiments. Here, we exploit boron-doped diamond (BDD) as microbead-based immunoassay be compared approach used commercial instrumentation. We conducted careful characterization signals tris(2,2'-bipyridine)ruthenium(II) (Ru(bpy)32+)/tri-n-propylamine (TPrA) system, both homogeneous (i.e., free diffusing Ru(bpy)32+) heterogeneous Ru(bpy)32+ bound microbeads). investigated promote TPrA oxidation, which enhancement intensity, results revealed that BDD properties greatly affect emission, so it does addition neutral, cationic, or anionic surfactants. Our show opposite outcomes, have practical consequences optimization. In conclusion, using Ru(bpy)32+-labeled immunoglobulins microbeads, resulted increase 70% double signal-to-noise ratio platinum electrodes, are actually instrumentation analysis. This research infers immunoassays higher can realized BDD.

Language: Английский

Citations

31
A fully integrated and handheld electrochemiluminescence device for detection of dopamine in bio-samples DOI
Lihang Zhu, Wenxuan Fu, Jing Chen

et al.

Sensors and Actuators B Chemical, Journal Year: 2022, Volume and Issue: 366, P. 131972 - 131972

Published: May 4, 2022

Language: Английский

Citations

27
Biochar: A Sustainable Alternative in the Development of Electrochemical Printed Platforms DOI Creative Commons
Rocco Cancelliere, Miriam Cianciaruso, Katya Carbone

et al.

Chemosensors, Journal Year: 2022, Volume and Issue: 10(8), P. 344 - 344

Published: Aug. 22, 2022

Biochar is a pyrolytic material with several environmental benefits such as reducing greenhouse gas emissions, sequestering atmospheric carbon and contrasting global warming. However, nowadays, it has moved to the forefront for its conductivity electron transfer properties, finding applications in fabrication of electrochemical platforms. In this field, researchers have focused on low-cost biomass capable replacing more popular expensive carbonaceous nanomaterials (i.e., graphene, nanotubes quantum dots) realization sensitive cost-effectiveness eco-friendly tools. This review discusses recent developments biochar-modified screen-printed electrodes (SPEs). Special attention been paid biochar’s manufacturing processes, electron-donating capabilities sensing applications. Examples representative works are introduced explain distinct roles biochar electro-bioanalytical strategies.

Language: Английский

Citations

26
Aspects of Electrochemical Biosensors Using Affinity Assays DOI Creative Commons

Thor Pedersen,

Leonid Gurevich, Nils E. Magnusson

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(3), P. 166 - 166

Published: March 4, 2025

In recent decades, the utilization of biomarkers has gained increasing attention. The timely identification and quantification proteins, nucleic acids, small molecules associated with a medical condition, infection, or contaminant have become increasingly crucial across variety fields, including medicine, food safety, quality/environmental control. State-of-the-art biomarker detection methods predominantly rely on standard immunoassay techniques, requiring specialized laboratory equipment trained personnel. This impedes broad commercial implementation biosensors in, e.g., Point-of-Care (PoC) settings where ease operation, portability, cost-efficiency are prioritized. Small, robust electrochemical promising alternative for analyzing in complex samples within PoC environments. Therefore, creating designing optimized sensing surfaces, immobilization strategies, efficient signal generation improving biosensor systems, which turn can real-world impact. present paper, we reviewed common electrode types geometries used approaches, discussed advantages drawbacks different methods, presented labeling strategies enhancement.

Language: Английский

Citations

0
Electrochemical Ion-exchange Investigation of Novel Hydrogel Functionalized Screen-printed Carbon Electrodes DOI
Jie Sun, Alexandre Da Silva, Sophie Griveau

et al.

Electrochimica Acta, Journal Year: 2025, Volume and Issue: unknown, P. 146361 - 146361

Published: May 1, 2025

Language: Английский

Citations

0
Emission from the working and counter electrodes under co-reactant electrochemiluminescence conditions DOI Creative Commons

Natasha S. Adamson,

Ashton G. Theakstone, Lachlan C. Soulsby

et al.

Chemical Science, Journal Year: 2021, Volume and Issue: 12(28), P. 9770 - 9777

Published: Jan. 1, 2021

Exploration of potential-dependent, multi-colour co-reactant electrochemiluminescence from multiple luminophores at the working and counter electrodes reveals new pathways to emission.

Language: Английский

Citations

23
Towards a Point-of-Care (POC) Diagnostic Platform for the Multiplex Electrochemiluminescent (ECL) Sensing of Mild Traumatic Brain Injury (mTBI) Biomarkers DOI Creative Commons
Milica Jović, Denis Prim,

Edis Saini

et al.

Biosensors, Journal Year: 2022, Volume and Issue: 12(3), P. 172 - 172

Published: March 11, 2022

Globally, 70 million people are annually affected by TBI. A significant proportion of all TBI cases actually mild (concussion, 70-85%), which is considerably more difficult to diagnose due the absence apparent symptoms. Current clinical practice diagnosing mTBI largely resides on patients' history, aspects, and CT MRI neuroimaging observations. The latter methods costly, time-consuming, not amenable for decentralized or accident site measurements. As an alternative (and/or complementary), diagnostics can be performed detection biomarkers from blood. Herein, we proposed two strategies three mTBI-relevant (GFAP, h-FABP, S100β), in standard solutions human serum samples using electrochemiluminescence (ECL) immunoassay (i) a commercial ECL platform 96-well plate format, (ii) "POC-friendly" with disposable screen-printed carbon electrodes (SPCE) portable reader. We further demonstrated proof-of-concept integrating individually developed assays ("

Language: Английский

Citations

15
Large-scale nano-biosensing technologies DOI Creative Commons
Ioulia Tzouvadaki, Themistoklis Prodromakis

Frontiers in Nanotechnology, Journal Year: 2023, Volume and Issue: 5

Published: Feb. 15, 2023

Nanoscale technologies have brought significant advancements to modern diagnostics, enabling unprecedented bio-chemical sensitivities that are key disease monitoring. At the same time, miniaturized biosensors and their integration across large areas enabled tessellating these into high-density biosensing panels, a capability for development of high throughput monitoring: multiple patients as well analytes per patient. This review provides critical overview various nanoscale ability unlock testing without compromising detection resilience. We report on challenges opportunities each technology presents along this direction present detailed analysis prospects both commercially available emerging technologies.

Language: Английский

Citations

7
Effect of Boron-Doping Level and Surface Termination in Diamond on Electrogenerated Chemiluminescence DOI

Kohei Sakanoue,

Andrea Fiorani,

Irkham Irkham

et al.

ACS Applied Electronic Materials, Journal Year: 2021, Volume and Issue: 3(9), P. 4180 - 4188

Published: Sept. 13, 2021

Electrogenerated chemiluminescence (ECL) is a phenomenon of light emission from molecular species that triggered by an electrochemical reaction. As with many systems, ECL highly dependent on the electrode material and its surface state. Boron-doped diamond (BDD) has been applied for ECL, however, thoughtful investigation remains scarce. In particular, peculiar feature BDD tuning properties changing amount boron doping which in turn affects response. Here, we summarize results most important such as Ru(bpy)32+ (bpy = 2,2′-bipyridine)/tri-n-propylamine (TPrA), Ru(bpy)32+/S2O82–, Ru(bpy)32+/SO42–, Ru(bpy)32+/CO32–, luminol/CO32– system, using correlating level to We showed suitable state are system investigated, highlighting importance right choice electrode. While focused these offer general interpretation might be adapted different systems where finds application.

Language: Английский

Citations

16