Effects of vaginal microbiota on in vitro fertilization outcomes in women with different infertility causes

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 27, 2025

ABSTRACT Backed by advancements in technologies like microbial sequencing, many studies indicate that the vaginal microbiome is a key marker of female reproductive health. However, further are still needed to investigate correlation between microbiota (VMB) and outcomes assisted technology (ART). Therefore, this study compared VMB two types infertile women undergoing vitro fertilization (IVF) with normal control during implantation window period investigated effects characteristics on IVF outcomes. Vaginal swabs from patients ( n = 85) were collected before embryo transfer (ET), 37) 6–8 days ovulation detection. Results showed differences community structure polycystic ovary syndrome (PCOS) tubal factor infertility (TFI) treatment. The results revealed higher relative abundance Lactobacillus iners non-pregnant group pregnant group. also demonstrated Pseudomonas spp. both groups women. findings suggested composition affects pre-ET high L. may potentially an failure. vagina be adverse for ART pregnancy. IMPORTANCE Many suggest affect fertilization-embryo (IVF-ET) Assessing can optimize timing better (ART) results. This examined distribution using 16S rRNA sequencing. impacted ([less than or equal to] 24 hours). identified as factors post-ET PCOS TFI ovulatory window. These highlight importance considering personalize treatment based type, improving chances success.

Language: Английский

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Gut–X axis

iMeta, Journal Year: 2025, Volume and Issue: 4(1)

Published: Feb. 1, 2025

Abstract Recent advances in understanding the modulatory functions of gut and microbiota on human diseases facilitated our focused attention contribution to pathophysiological alterations many extraintestinal organs, including liver, heart, brain, lungs, kidneys, bone, skin, reproductive, endocrine systems. In this review, we applied “gut–X axis” concept describe linkages between other organs discussed latest findings related axis,” underlying mechanisms potential clinical intervention strategies.

Language: Английский

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Editorial: New techniques in microbiome research - volume II: Host-microbiome interactions using ‘meta-omics’ techniques

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Feb. 5, 2025

influence human metabolic pathways, offering potential avenues for early diagnosis and targeted therapy.Therapeutic Interventions. Understanding microbiome-disease associations has led to the exploration of microbiome-based therapies. Probiotic prebiotic interventions, as well fecal microbiota transplantation (FMT), are being investigated their restore healthy microbiome composition mitigate disease progression. FMT gained traction a treatment recurrent Clostridioides difficile (C. difficile) infections been explored its role in other conditions such IBD (5).The impact on cancer one most intriguing areas research. Studies suggest that can progression, immune response, even efficacy For example, research shown microbial species residing tumor microenvironment modulate checkpoints affect response immunotherapy. have certain species, Fusobacterium nucleatum, exacerbate colorectal by promoting inflammation genomic instability (6).Microbiome neurological disorders. The gut-brain axis become focal point into neurodegenerative diseases. Meta-omics approaches gut dysbiosis health altering metabolite production, which turn affects neuroinflammation brain function (7). This emerging evidence suggests may play crucial development diseases Parkinson's Alzheimer's, new opportunities therapies.The profoundly impacts susceptibility defense. Dysbiosis body gut, vaginal, skin microbiomes is associated with an increased risk (8). Similarly, compromises function, leading greater gastrointestinal infections, including C. infection (9). Research also highlights gut-lung axis, showing imbalances respiratory suggesting systemic maintaining homeostasis Moreover, sputum severe critically ill influenza patients (10), In addition, indicates alter SARS-CoV-2 virus load COVID-19 severity (11).Microbiome women health. plays critical women's health, particularly context vaginal microbiomes. microbiome, typically dominated Lactobacillus essential environment producing lactic acid acidic pH. Disruptions this balance result bacterial vaginosis, yeast sexually transmitted (STIs) (12). cases vaginosis dysbiosis, increase pathogens like Gardnerella vaginalis Prevotella linked adverse reproductive outcomes, infertility miscarriage (12)(13).In oral changes menstrual cycle. Different profiles were observed during follicular phase, late luteal phases. Alpha diversity beta analyses revealed distinct across four phases (14). lactobacilli used (15). urinary signatures different types diversion (16).Furthermore, significant implications hormonal regulation responses, polycystic ovary syndrome (PCOS) pregnancy outcomes. alterations insulin resistance, imbalances, PCOS, contributing symptoms disturbances (12) (13).The aim Topic offer platform articles expand our understanding host-microbiome interactions using 'meta-omics' techniques. collection includes 5 reviews 14 original articles, highlighting between various These contributions made strides advancing knowledge complex interplay states, cancers (Lin et al. Microbial influences mechanism, prognosis, diagnosis, therapy. A comprehensive review examines intratumoral mechanisms, treatment. It delves roles microbiota, emphasizing responses. Additionally, provides overview recent microbiome-cancer research, summarizing key findings from literature, past current advancements, discussing novel techniques, identifying gaps, outlining future goals. explores cancer, detailed analysis rapidly evolving field (Lin, al., 2025). tract lung investigates communities inhabiting regions system (Cheng, 2024). could mediating oncogenesis through potentially opening therapeutic interventions (Li, 2023). Colorectal (CRC)-associated Porphyromonas endodontalis, Ruminococcus torques, Odoribacter splanchnicus. stage-specific taxa identified, aid diagnosing polyps (BP) CRC stages (Cai Furthermore, specific pathways occurrence series immune-related events (irAEs) gastric, esophageal, colon cancers. Notably, callidus Bacteroides xylanisolvens enriched without irAEs. Several citrulline arginine biosynthesis, irAE (Zhang 2023).The authors reviewed innovative single cell sequencing broad applications addressing questions regarding spatial heterogeneity. advancements deeper insights functional individual cells within (Madhu, etal., microbiota-gut-brain pivotal regulating neuroprotective functions host. Patients (PD) often exhibit (Jia, he appear long-term depression (Li identification classes Negativicutes Selenomonadales direction strategies manage sleep disorders via modulation (Wang, another study, ten (GM) found causal insomnia 2023).Oral During relative abundance Streptococcus genus was significantly higher compared phases, while 7 6 genera exhibited lower phase than notably (Yamazaki 2023).Use probiotic infections. Lactobacilli microenvironment. treating female contribute restoring preserving competing pathogenic bacteria, enhancing local antimicrobial substances (Liu, diversion. studied. Urinary landscapes radical cystectomy (UD) analyzed 2024).Sputum characteristics patients. Bacteroidetes showed depletion cohort. group marked overrepresentation Neisseria, Porphyromonas, Actinobacillus, Alloprevotella, Nanosynbacter lyticus TM7x, Clostridia UCG-014. Alloprevotella inverse correlation cycle threshold (Ct) values. C-reactive protein (CRP) levels demonstrated positive presence Haemophilus (Gu, infection. Distinct patterns identified among individuals, colonized patients, those (CDI), (R-CDI), non-Clostridioides (NOCDI) diarrhea. Potential biomarkers discovered be valuable distinguishing true CDI conditions, improving diagnostic accuracy guiding (Vázquez-Cuesta, 2023).Gut children split-dose bowel preparations. pediatric undergoing PEG preparation colonoscopy, at genus, pathway levels. However, no phylum level (Zou, 2023).Correlations fungal clinical parameters Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD) (Niu, One study reveals differences cholesterol pigment gallstone 2024).A combination NaClO H₂O₂ more effective disinfectant, against pathogens, alternative solution efficient control 2022).In conclusion, integration meta-omics techniques revolutionized ability explore microbiome's diseases, providing As these continue evolve, they hold immense promise targets wide range

Language: Английский

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Vaginal and Cervical Microbiota Composition in Patients with Endometrial Cancer

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(9), P. 8266 - 8266

Published: May 5, 2023

According to recent data, changes in the vaginal microbiota could affect risk of gynaecological cancers. Women suffering from endometrial cancer present significant cervicovaginal composition. The objective our study was characterize women undergoing hysterectomy due benign disease, atypical hyperplasia, and cancer; included 96 patients, who undergone surgical treatment uterine precancerous lesion, cancer. Quantitative qualitative real-time PCR analysis DNA isolated fornix endocervical canal samples performed detect 19 most commonly identified microorganisms, including different Lactobacillus spp., Atopobium, Bifidobacterium, Chlamydia, Gardnerella; At least one tested microorganisms 88.5% 83.3% cervical samples. iners significantly more frequent patients with condition, whereas Dialister pneumosintes Mobiluncus curtisii patients; curtisi pneumosintes, which were as common samples, may be considered potential co-factors promote/stimulate carcinogenesis. However, exact mechanism such activity remains unexplained requires further investigations.

Language: Английский

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Impact of Probiotics and Prebiotics on Gut Microbiome and Hormonal Regulation

Gastrointestinal Disorders, Journal Year: 2024, Volume and Issue: 6(4), P. 801 - 815

Published: Sept. 27, 2024

The gut microbiome plays a crucial role in human health by influencing various physiological functions through complex interactions with the endocrine system. These involve production of metabolites, signaling molecules, and direct communication cells, which modulate hormone secretion activity. As result, can exert neuroendocrine effects contribute to metabolic regulation, adiposity, appetite control. Additionally, influences reproductive altering levels sex hormones such as estrogen testosterone, potentially contributing conditions like polycystic ovary syndrome (PCOS) hypogonadism. Given these roles, targeting offers researchers clinicians novel opportunities improve overall well-being. Probiotics,

Language: Английский

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Vaginal microbiome in obesity and its impact on reproduction

Best Practice & Research Clinical Obstetrics & Gynaecology, Journal Year: 2023, Volume and Issue: 90, P. 102365 - 102365

Published: June 13, 2023

A number of reproductive outcomes have been increasingly found to be affected by the vaginal microbiota. Obesity has become a global epidemic, affecting increasing numbers reproductive-age women, and shown risk factor for adverse female health outcomes. healthy microbiome is characterized Lactobacillus-dominance, in particular Lactobacillus crispatus; obesity associated with higher diversity lower likelihood Lactobacillus-dominance. In this review, we summarize evidence on obese women impact such as conception rates, early pregnancy, preterm birth. We further explore mechanisms which may result an altered microbial composition highlight future avenues therapeutic targeting

Language: Английский

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Vaginal and endometrial microbiome dysbiosis associated with adverse embryo transfer outcomes

Reproductive Biology and Endocrinology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Aug. 28, 2024

Assisted reproductive technology (ART) is the most effective method to treat infertility and pathogenesis of implantation failure after in vitro fertilization-embryo transfer (IVF-ET) a challenging filed infertility. Microbes female tract are considered be associated with gynecological obstetric diseases. However, its effects on embryo unsured.

Language: Английский

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Elevated LINC00115 expression correlates with aggressive endometrial cancer phenotypes via JAK/STAT pathway modulation

Human Molecular Genetics, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

Abstract This study systematically explores the oncogenic role of long non-coding RNA (lncRNA) LINC00115 in endometrial cancer (EC) and reveals its unique mechanism promoting proliferation, invasion, metastasis via JAK/STAT signaling pathway. is significantly upregulated EC tissues closely associated with advanced TNM staging lymph node metastasis. Functional assays showed that knockdown suppressed cell metastasis, while overexpression enhanced these malignant behaviors. In vivo models confirmed accelerates tumor growth Our first to identify as a key activator pathway through direct interaction KH-type Splicing Regulatory Protein (KHSRP), previously unrecognized EC. finding provides new insights into lncRNA-mediated regulation highlights novel biomarker promising therapeutic target for EC, offering theoretical basis developing targeted therapies.

Language: Английский

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Sex Hormones–Gut Microbiome Axis: An Update of What Is Known So Far

Current Opinion in Endocrine and Metabolic Research, Journal Year: 2025, Volume and Issue: unknown, P. 100571 - 100571

Published: Jan. 1, 2025

Language: Английский

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Vaginal Dysbiosis in Infertility: A Comparative Analysis Between Women with Primary and Secondary Infertility

Microorganisms, Journal Year: 2025, Volume and Issue: 13(1), P. 188 - 188

Published: Jan. 17, 2025

Infertility, both primary and secondary, is strongly influenced by microbiological factors, with the vaginal microbiota playing a key role in reproductive health. The aim of this study was to characterize 136 Mexican women diagnosed infertility-primary (n = 58) secondary 78)-by evaluating presence pathogenic bacterial species their associations infertility conditions. Samples were obtained through cervical swabs, microorganism identification performed using qPCR techniques. Analysis revealed positive correlation between increased age likelihood infertility, as well negative infertility. Significant differences microbial composition also observed two groups. Lactobacillus crispatus gasseri dominant addition high prevalence Gardnerella vaginalis Fannyhessea vaginae. Additionally, correlations found human papillomavirus (HPV) sexually transmitted bacteria, vaginalis. Our findings suggest that may play decisive highlighting need for personalized therapeutic strategies based on profiles.

Language: Английский

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