Microorganisms,
Journal Year:
2024,
Volume and Issue:
12(4), P. 704 - 704
Published: March 30, 2024
Pathogenic
biofilms
provide
a
naturally
favorable
barrier
for
microbial
growth
and
are
closely
related
to
the
virulence
of
pathogens.
Postbiotics
from
lactic
acid
bacteria
(LAB)
secondary
metabolites
cellular
components
obtained
by
inactivation
fermentation
broth;
they
have
certain
inhibitory
effect
on
all
stages
pathogen
biofilms.
LAB
drawn
attention
because
their
high
stability,
safety
dose
parameters,
long
storage
period,
which
give
them
broad
application
prospect
in
fields
food
medicine.
The
mechanisms
eliminating
via
postbiotics
mainly
affect
surface
adhesion,
self-aggregation,
virulence,
QS
pathogens
influencing
interspecific
intraspecific
communication.
However,
there
some
factors
(preparation
process
lack
target)
can
limit
antibiofilm
impact
postbiotics.
Therefore,
using
delivery
carrier
optimizing
interfering
be
eliminated.
This
review
summarizes
concept
characteristics
LAB,
focusing
preparation
technology
effect,
applications
limitations
processing
clinical
treatment
also
discussed.
Infection and Drug Resistance,
Journal Year:
2024,
Volume and Issue:
Volume 17, P. 449 - 462
Published: Feb. 1, 2024
Abstract:
Klebsiella
pneumoniae
(
K.
),
a
significant
contributor
to
the
global
challenge
of
antibiotic
resistance,
is
not
only
ubiquitous
component
human
microbiome
but
also
potent
pathogen
capable
causing
spectrum
diseases.
This
review
provides
thorough
analysis
intricate
interactions
between
and
immune
system,
elucidating
its
substantial
impact
on
metabolic
processes.
We
explore
mechanisms
employed
by
evade
manipulate
responses,
including
molecular
mimicry,
modulation,
biofilm
formation.
The
further
investigates
bacterium
'
s
influence
pathways,
particularly
glycolysis,
highlighting
how
these
exacerbate
disease
severity.
emergence
multidrug-resistant
extremely
drug-resistant
strains
within
Enterobacteriaceae
family
has
heightened
public
health
crisis,
underscoring
urgency
for
comprehensive
research.
investigate
roles
host's
complement
autophagy,
cell
death
mechanisms,
various
cytokines
in
combating
infections,
shedding
light
areas
that
warrant
academic
investigation.
Additionally,
discusses
challenges
posed
K1-
K2-capsule
polysaccharides
vaccine
development
due
their
complex
structures
adhesive
properties.
Acknowledging
limited
availability
effective
antimicrobials,
this
advocates
exploring
alternative
approaches
such
as
immunotherapeutics,
vaccinations,
phage
therapy.
consolidate
current
knowledge
,
covering
classical
non-classical
subtypes,
antimicrobial
resistance-mediated
genes,
virulence
factors,
epidemiological
trends
isolation
resistance
rates.
advances
our
understanding
underscores
imperative
ongoing
research
collaborative
efforts
develop
new
prevention
treatment
strategies
against
formidable
pathogen.
Keywords:
pathogenic
mechanism,
drug-resistant,
strategy
Microorganisms,
Journal Year:
2022,
Volume and Issue:
10(9), P. 1773 - 1773
Published: Sept. 2, 2022
The
increased
incidence
of
mixed
infections
requires
that
the
scientific
community
develop
novel
antimicrobial
molecules.
Essential
oils
and
their
bioactive
pure
compounds
have
been
found
to
exhibit
a
wide
range
remarkable
biological
activities
are
attracting
more
attention.
Therefore,
aim
this
study
was
evaluate
myrtenol
(MYR),
one
constituents
commonly
in
some
essential
oils,
for
its
potential
inhibit
biofilms
alone
combination
with
drugs
against
Candida
auris/Klebsiella
pneumoniae
single
biofilms.
activity
MYR
evaluated
by
determining
bactericidal/fungicidal
concentrations
(MIC),
biofilm
formation
at
sub-MICs
analyzed
96-well
microtiter
plate
crystal
violet,
XTT
reduction
assay,
CFU
counts.
synergistic
interaction
between
checkerboard
method.
exhibited
high
while
showing
efficient
antibiofilm
dual
To
understand
underlying
mechanism
which
promotes
single/mixed-species
inhibition,
we
observed
significant
downregulation
expression
mrkA,
FKS1,
ERG11,
ALS5
genes,
associated
bacterial
motility,
adhesion,
as
well
ROS
production,
can
play
an
important
role
inhibition
formation.
In
addition,
microdilution
assay
showed
strongly
both
caspofungin
(CAS)
meropenem
(MEM)
inhibiting
growth
pneumoniae-mixed
Furthermore,
tested
absence
toxicity
mammalian
cells
vitro
vivo
Galleria
mellonella
models.
Thus,
could
be
considered
agent
management
polymicrobial
iScience,
Journal Year:
2023,
Volume and Issue:
26(7), P. 107215 - 107215
Published: June 28, 2023
Developing
an
effective
therapy
to
overcome
carbapenemase-positive
Klebsiella
pneumoniae
(CPKp)
is
important
therapeutic
challenge
that
must
be
addressed
urgently.
Here,
we
explored
a
Ca-EDTA
combination
with
aztreonam
or
ceftazidime-avibactam
in
vitro
and
vivo
against
diverse
CPKp
clinical
isolates.
The
synergy
testing
of
this
study
demonstrated
novel
aztreonam-Ca-EDTA
ceftazidime-avibactam-Ca-EDTA
was
significantly
eliminating
planktonic
mature
biofilms
vitro,
as
well
eradicating
infections
vivo.
Both
combinations
revealed
significant
efficacies
reducing
bacterial
load
internal
organs
protecting
treated
mice
from
mortality.
Conclusively,
the
first
demonstrate
provide
favorable
efficacy
safety
for
successful
eradication
carbapenemase-producing
biofilm
infections.
Journal of Medicinal Chemistry,
Journal Year:
2023,
Volume and Issue:
66(16), P. 11555 - 11572
Published: Aug. 11, 2023
Antimicrobial
cationic
peptides
are
intriguing
and
propitious
antibiotics
for
the
future,
even
against
multidrug-resistant
superbugs.
Venoms
serve
as
a
source
of
cutting-edge
therapeutics
innovative,
unexplored
medicines.
In
this
study,
novel
peptide
library
consisting
seven
sequences
was
designed
synthesized
from
snake
venom
cathelicidin,
batroxicidin
(BatxC),
with
inclusion
FLPII
motif
at
N-terminus.
SP1V3_1
demonstrated
exceptional
antibacterial
effectiveness
Escherichia
coli,
methicillin-resistant
Staphylococcus
aureus
(MRSA),
Pseudomonas
aeruginosa,
Klebsiella
pneumoniae
destroyed
bacteria
by
depolarizing,
rupturing,
permeabilizing
their
membranes,
evident
fluorescence
assays,
atomic
force
microscopy,
scanning
electron
microscopy.
observed
to
modulate
immune
response
in
LPS-elicited
U937
cells
exhibited
good
antibiofilm
activity
MRSA
K.
pneumoniae.
The
promoted
wound
healing
disinfection
murine
model.
study
that
is
an
exciting
lead
may
be
explored
further
development
better
therapeutic
peptides.
Microorganisms,
Journal Year:
2024,
Volume and Issue:
12(4), P. 704 - 704
Published: March 30, 2024
Pathogenic
biofilms
provide
a
naturally
favorable
barrier
for
microbial
growth
and
are
closely
related
to
the
virulence
of
pathogens.
Postbiotics
from
lactic
acid
bacteria
(LAB)
secondary
metabolites
cellular
components
obtained
by
inactivation
fermentation
broth;
they
have
certain
inhibitory
effect
on
all
stages
pathogen
biofilms.
LAB
drawn
attention
because
their
high
stability,
safety
dose
parameters,
long
storage
period,
which
give
them
broad
application
prospect
in
fields
food
medicine.
The
mechanisms
eliminating
via
postbiotics
mainly
affect
surface
adhesion,
self-aggregation,
virulence,
QS
pathogens
influencing
interspecific
intraspecific
communication.
However,
there
some
factors
(preparation
process
lack
target)
can
limit
antibiofilm
impact
postbiotics.
Therefore,
using
delivery
carrier
optimizing
interfering
be
eliminated.
This
review
summarizes
concept
characteristics
LAB,
focusing
preparation
technology
effect,
applications
limitations
processing
clinical
treatment
also
discussed.
