Caracterização de proteínas recombinantes de Rickettsia rickettsii para diagnóstico sorológico em suídeos DOI Creative Commons

Maribel Loza

Published: Aug. 25, 2023

Brazilian Spotted Fever (BSF) is a disease strongly associated with wild boars (Sus scrofa) as its primary hosts, caused by the etiological agent Rickettsia rickettsii.It an endemic zoonosis primarily transmitted to humans through bite of ticks Amblyomma genus, presenting high fatality rate (≥ 50%), especially in southeastern region country.Studies have shown that 72.5% boars, 14.1% hunting dogs, and 14.7% hunters exhibited seropositivity for at least one species Rickettsia.The importance proteins found outer membrane Gram-negative bacteria like R. rickettsii crucial physiological functions, virulence, bacterial resistance, serving cellular support.In this study, we evaluated IgG reactivity rOmpA, rPhenAl, rYJBL using ELISA well-characterized boar serum panel, laboratory-confirmed diagnosis indirect immunofluorescence (IFA).We positivity recombinant samples (97.2% rPhenAl rYJBL; 94.4% rOmpA), indicating these potential antigen candidates various immunoassays be applied detection specific antibodies suids other wildlife naturally exposed sp. immunogens future research development vaccines against rickettsioses.

Language: Английский

Discovery of Kaempferol, a Novel ADAM10 Inhibitor, as a Potential Treatment for Staphylococcus aureus Infection DOI Creative Commons
Tingting Wang, Jianfeng Wang,

Xiangzhu Xu

et al.

Engineering, Journal Year: 2023, Volume and Issue: 28, P. 206 - 221

Published: April 14, 2023

Host-directed therapy (HDT) is an emerging novel approach for treating multidrug-resistant Staphylococcus aureus (S. aureus) infection. Functioning as the indispensable specific cellular receptor α-toxin (Hla), a-disintegrin and metalloproteinase 10 (ADAM10) exploited to accelerate S. infection through diverse mechanisms. The extraordinary contribution of ADAM10 pathogenesis renders it attractive HDT target combating Our study first demonstrate role in aureus-induced necroptosis, enhances our knowledge Using a fluorogenic substrate assay, we further identified kaempferol potent inhibitor that effectively protected mice from by suppressing Hla-mediated barrier disruption necroptosis. Collectively, work presents host-directed therapeutic strategy using promising candidate treat other diseases relevant disordered upregulation ADAM10.

Language: Английский

Citations

4
Editorial: Innate immunity against intracellular bacteria: mechanisms and strategies DOI Creative Commons

Jingfei Tian,

Han Liu,

Jingai Che

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: March 20, 2024

Keywords: autophagy, pyroptosis, pathogen-associated molecular patterns, antimicrobial peptides, phagocytic cells

Language: Английский

Citations

1
Rickettsia helvetica in C3H/HeN mice: a model for studying pathogen- host interactions DOI Creative Commons
Apolline Maître, Lourdes Mateos‐Hernández, Tal Azagi

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(18), P. e37931 - e37931

Published: Sept. 1, 2024

Language: Английский

Citations

1
Spotted fever rickettsia-induced microvascular endothelial barrier dysfunction is delayed by the calcium channel blocker benidipine DOI Creative Commons
Yuri Kim,

Emily G. Clemens,

Jennifer M. Farner

et al.

Biochemical and Biophysical Research Communications, Journal Year: 2023, Volume and Issue: 663, P. 96 - 103

Published: April 24, 2023

Language: Английский

Citations

1
The intricate dance: host autophagy and Coxiella burnetii infection DOI Creative Commons
Tingting Wang, Chao Wang, Chang Li

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Sept. 22, 2023

Q fever is a zoonotic disease caused by Coxiella burnetii, an obligatory intracellular bacterial pathogen. Like other pathogens, C. burnetii able to survive and reproduce within host cells manipulating cellular processes. In particular, the relationship between infection autophagy, process involved in degradation recycling, of great interest due its intricate nature. Studies have shown that autophagy can recognize target pathogens such as Legionella Salmonella for degradation, limiting their replication promoting clearance. However, actively manipulate autophagic pathway create niche, known Coxiella-containing vacuole (CCV), where it multiply evade immune responses. promotes fusion CCVs with lysosomes through mechanisms involving virulence factors Cig57 CvpF. This review summarizes latest findings on dynamic interaction infection, highlighting complex strategies employed both bacterium host. A better understanding these could provide important insights into development novel therapeutic interventions vaccine against infections.

Language: Английский

Citations

1
Anaphylatoxin signaling activates macrophages to control intracellular Rickettsia proliferation DOI Creative Commons
Mustapha Dahmani, Jinyi C. Zhu,

Jack H. Cook

et al.

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(6)

Published: Oct. 19, 2023

Pathogenic Rickettsia species are extremely dangerous bacteria that grow within the cytoplasm of host mammalian cells. In most cases, these able to overpower cell and protected environment cytoplasm. However, a dramatic conflict occurs when encounter innate immune cells; can "win" by taking over host, or "lose" if efficiently fights infection. This manuscript examines how complement system is detect presence alert nearby Byproducts activation called anaphylatoxins signals "activate" cells mount an aggressive defensive strategy. study enhances our collective understanding reaction intracellular will contribute future efforts at controlling infections.

Language: Английский

Citations

1
Genomic characterization of an emerging Rickettsia barbariae isolated from tick eggs in northwestern China DOI Creative Commons
Ning Wang, Huijun Yu,

Xiaoyu Han

et al.

Emerging Microbes & Infections, Journal Year: 2024, Volume and Issue: 13(1)

Published: Aug. 28, 2024

The continual emergence of tick-borne rickettsioses has garnered widespread global attention.

Language: Английский

Citations

0
Caracterização de proteínas recombinantes de Rickettsia rickettsii para diagnóstico sorológico em suídeos DOI Creative Commons

Maribel Loza

Published: Aug. 25, 2023

Brazilian Spotted Fever (BSF) is a disease strongly associated with wild boars (Sus scrofa) as its primary hosts, caused by the etiological agent Rickettsia rickettsii.It an endemic zoonosis primarily transmitted to humans through bite of ticks Amblyomma genus, presenting high fatality rate (≥ 50%), especially in southeastern region country.Studies have shown that 72.5% boars, 14.1% hunting dogs, and 14.7% hunters exhibited seropositivity for at least one species Rickettsia.The importance proteins found outer membrane Gram-negative bacteria like R. rickettsii crucial physiological functions, virulence, bacterial resistance, serving cellular support.In this study, we evaluated IgG reactivity rOmpA, rPhenAl, rYJBL using ELISA well-characterized boar serum panel, laboratory-confirmed diagnosis indirect immunofluorescence (IFA).We positivity recombinant samples (97.2% rPhenAl rYJBL; 94.4% rOmpA), indicating these potential antigen candidates various immunoassays be applied detection specific antibodies suids other wildlife naturally exposed sp. immunogens future research development vaccines against rickettsioses.

Language: Английский

Citations

0