Investigating the association between Candida albicans and early childhood dental caries: A comprehensive systematic review and meta-analysis DOI Creative Commons
Lusine Khachatryan,

Fatemeh Allahbakhsi,

Diana E. Vadiyan

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(12), P. e0315086 - e0315086

Published: Dec. 16, 2024

Background Early childhood caries (ECC) is a significant public health concern affecting children globally. Recent studies suggest potential association between the presence of Candida albicans (C . albicans) in oral cavity and risk ECC, but findings have been inconsistent. This systematic review meta-analysis aimed to investigate C ECC. Methods A comprehensive literature search was conducted across databases including PubMed, Web Science, Cochrane Central Register Controlled Trials, Embase, Google Scholar, Scopus. Studies were included if they examined occurrence or severity dental children. Data extraction quality assessment performed independently by two reviewers. Statistical analyses, pooled odds ratios (OR) 95% confidence intervals (CI), synthesize findings. Results total 22 studies, involving 3301 participants, meta-analysis. The revealed an increased likelihood with ratio 4.42 (95% CI: 3.14–6.24, p < 0.001). Subgroup analyses showed that factors such as geographic region, study design, detection methods influenced strength this association. Europe reported stronger (OR: 10.13, 6.01–17.09) compared those Asia 3.62, 2.53–5.20) Americas 3.98, 1.14–13.87). Case-control had higher OR (5.30, 1.72–16.31) cross-sectional (4.30, 2.92–6.33). ECC 4.08 2.65–6.27) plaque samples, 9.55 2.17–42.01) swab 4.70 2.44–9.09) saliva samples. No publication bias observed based on Begg’s test (P-value = 0.612) Egger’s 0.250). Conclusions provide support for hypothesis plays role development Moving forward, it essential future research concentrate comprehending mechanisms underlie relationship develop specific strategies prevention.

Language: Английский

Effects of Green Tea Extract Epigallocatechin-3-Gallate on Oral Diseases: A Narrative Review DOI Creative Commons
Yizhen Li, Lei Cheng, Mingyun Li

et al.

Pathogens, Journal Year: 2024, Volume and Issue: 13(8), P. 634 - 634

Published: July 29, 2024

Oral diseases are among the most prevalent globally. Accumulating new evidence suggests considerable benefits of epigallocatechin-3-gallate (EGCG) for oral health. This review aims to explore role and application EGCG in main diseases.

Language: Английский

Citations

7
Fluoride export is required for the competitive fitness of pathogenic microorganisms in dental biofilm models DOI Creative Commons
Aditya Banerjee, Chia-Yu Kang, Minjun An

et al.

mBio, Journal Year: 2024, Volume and Issue: 15(5)

Published: April 16, 2024

ABSTRACT Microorganisms resist fluoride toxicity using export proteins from one of several different molecular families. Cariogenic species Streptococcus mutans and Candida albicans extrude intracellular a CLC F - /H + antiporter FEX channel, respectively, whereas oral commensal eubacteria, such as gordonii, Fluc channel. In this work, we examine how genetic knockout impacts pathogen fitness in single-species three-species dental biofilm models. For biofilms generated S. with the transporter, exposure to low concentrations decreased counts, synergistically reduced populations C. , increased relative proportion gordonii properties associated pathogenicity, including acid production hydroxyapatite dissolution. Biofilms prepared channel also exhibited presence but lesser degree. Imaging studies indicate that is highly sensitive fluoride, strain undergoing complete lysis when exposed for moderate amount time. Biochemical purification transporter functional reconstitution establishes protein dimer encoded by single gene. Together, these findings suggest pathogens can be targeted specific inhibitors restore symbiosis especially susceptible toxicity. IMPORTANCE Dental caries globally prevalent condition occurs pathogenic species, outcompete beneficial biofilm. Fluoride routinely used hygiene prevent caries. has antimicrobial properties, although most microbes possess exporters its This work shows sensitization cariogenic alters microbial composition biofilms. These results development drugs inhibit could potentiate anticaries effect over-the-counter products like toothpaste mouth rinses. novel strategy treat

Language: Английский

Citations

5
Droplet microfluidics: unveiling the hidden complexity of the human microbiome DOI

Yibin Xu,

Zhiyi Wang, Caiming Li

et al.

Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

The human microbiome is vital for health. Droplet microfluidics offers a versatile toolbox research, enabling single-cell sequencing, cultivation, and functional analyses to deepen our understanding drive innovations.

Language: Английский

Citations

0
V-ATPase contributes to the cariogenicity of Candida albicans- Streptococcus mutans biofilm DOI Creative Commons
Jiang Qian,

Mengmeng Xu,

Hong Chen

et al.

npj Biofilms and Microbiomes, Journal Year: 2025, Volume and Issue: 11(1)

Published: March 8, 2025

The interaction between Candida albicans and Streptococcus mutans plays an important role in the progression of dental caries. vacuolar proton pump (V-ATPase) is a vital enzyme regulating growth virulence C. albicans, which potential target for caries prevention. However, effect V-ATPase on cariogenicity albicans-S. biofilm remains to be explored. In this study, detection rate caries-active (group CA) (22.03%) was significantly higher than that caries-free CF) children (8.00%), expression related genes were group CA. Then, expressed coding VMA3, VMA4 VMA11 CA knocked out. Compared with wild type SC5314, mutants showed slower rate, inhibited hyphal growth, defective integrity cell wall. biomass extracellular polysaccharide (EPS) production dual reduced, structure impacted. Transcriptome analysis indicated participated various metabolisms biosynthesis pathways influenced EPS metabolism S. mutans. Finally, compared positive control, severity, plaque reduced after deletion vivo. This study revealed first time its mechanisms. results may provide basis new strategies ecological prevention treatment

Language: Английский

Citations

0
Suppressive effects of lemon myrtle extract against the colonization and virulence factors of Candida spp. DOI
Siyuan Liu, Yi Wang, C. F. Xu

et al.

Journal of Oral Biosciences, Journal Year: 2025, Volume and Issue: unknown, P. 100657 - 100657

Published: March 1, 2025

Language: Английский

Citations

0
Candida albicans impacts carbohydrate metabolism of Enterococcus faecalis in interkingdom biofilms DOI Creative Commons
Nicole de Mello Fiallos, I. Santos, Debra N. Brunson

et al.

Journal of Oral Microbiology, Journal Year: 2025, Volume and Issue: 17(1)

Published: April 16, 2025

This study investigated the transcriptional and physiological responses of Enterococcus faecalis, an opportunistic pathogen linked to endodontic infections, when cultivated in dual-species biofilms with Candida albicans, a yeast pathobiont found oral cavity. Forty-eight-hour E. faecalis OG1RF were developed BHI medium as mono- or C. albicans SC5314. Biofilms assessed for biomass, colony-forming units (CFUs), architecture using confocal microscopy. RNA sequencing was performed on Illumina platform. Mannose-PTS activity glycerol quantification assays conducted investigate changes carbohydrate metabolism. Transcriptomic analysis revealed 149 genes differentially expressed biofilms. Genes mannose-PTS metabolism notably upregulated. significantly higher Mannose, sole source, increased CFUs decreased co-culture, while glucose had no effect. As is net producer, levels always present, likely contributing upregulation co-cultures. The presence alters gene expression metabolism, suggesting metabolic crosstalk that may influence their pathogenicity role infections.

Language: Английский

Citations

0
Novel two-stage expansion of Streptococcus mutans biofilm supports EPS-targeted prevention strategies for early childhood caries DOI Creative Commons

Jinseon Moon,

Kwang‐Suk Seo, Jae‐Sung Kwon

et al.

npj Biofilms and Microbiomes, Journal Year: 2025, Volume and Issue: 11(1)

Published: April 24, 2025

Early childhood caries (ECC) affects nearly half of preschool children worldwide and characterized by rapid progression across multiple teeth. While Streptococcus mutans (S. mutans) is a keystone species in dental caries, its process for biofilm expansion remains unclear. Using an air-solid interface model simulating the oral environment, we uncovered novel S. biofilms. Our findings reveal that employs distinct two-step strategy. Through osmotic pressure, extracellular polymeric substances (EPS) spread transport bacterial clusters to new sites. Subsequently, hydroxyapatite surface enables colony formation. Hydroxyapatite's acid-neutralization properties appear critical growth colonization. Despite successful EPS spreading, environments without failed support These results unique pattern ECC sugar-rich establish as promising therapeutic target, advancing understanding cariogenic behavior preventative strategies prevention.

Language: Английский

Citations

0
Antimicrobial and Antibiofilm Properties of Hydroxyapatite/Nano-Hydroxyapatite in Preventing Dental Caries: A Systematic Review DOI Creative Commons
Nurdiana Dewi, Meirina Gartika,

Dwi Gustiono

et al.

European Journal of Dentistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Controlling biofilm is a crucial strategy and an essential component of preventing dental caries. Considerable research has been conducted in recent years on the clinical application hydroxyapatite (HAp) nanoparticles (nHAp) However, these studies have yet to investigate effectiveness or mechanism substances as antibacterial antibiofilm agents. This study aimed provide thorough analysis current evidence characteristics HAp/nHAp prevention Searches were across five databases: Cochrane Library, PubMed, Scopus, EBSCOhost, ScienceDirect. Google Scholar was also searched. Titles, abstracts, full text evaluated following guidelines set by Preferred Reporting Item for Systematic Review Meta-Analyses (PRISMA). A methodological quality assessment using QUIN tool. The initial retrieval totaled 15,047 studies, from which 3,487 excluded. total 11,560 screened based title abstract, resulting 24 full-text considered potentially eligible inclusion (κ = 0.9599). Finally, 19 met all defined criteria included this comprehensive systematic review 0.8837). demonstrates antimicrobial activities against gram-negative gram-positive bacteria fungi. nHAp's efficacy remains limited. Further investigation required improve

Language: Английский

Citations

0
Decoding Early Childhood Caries: A Comprehensive Review Navigating the Impact of Evolving Dietary Trends in Preschoolers DOI Open Access
Kanika Singh Dhull, Brahmananda Dutta,

Sushmita Pattanaik

et al.

Cureus, Journal Year: 2024, Volume and Issue: unknown

Published: April 13, 2024

This comprehensive review delves into the intricate relationship between evolving dietary trends in preschoolers and prevalence of early childhood caries (ECC). The investigation meticulously analyzes ECC epidemiology, etiology, preventive strategies. unveils multifaceted nature ECC, highlighting microbial, dietary, environmental factors contributing to its development. Significantly, study explores global substantial implications for overall health, nutrition, development preschool-aged children. public health policy are deliberated, advocating targeted interventions collaborative efforts among healthcare professionals, policymakers, educators, parents. conclusion presents a compelling call action, urging collective engagement mitigate impact prioritize well-being preschoolers. offers valuable insights parents inform evidence-based strategies addressing promoting oral health.

Language: Английский

Citations

3
Association between Salivary Immune Markers and Oral Health Conditions in Pregnant Women DOI
Nora Alomeir, Xueying Mao,

Ruqian Yang

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 14, 2025

Abstract Objectives: This study aims to assess levels of salivary immune markers in women during the 3rd trimester pregnancy and explores association between dental caries, as well oral carriage pathogens Candida albicans Streptococcus mutans. These organisms are known be associated with infectious diseases such caries candida infection. Methods: Pregnant from underserved background participated this (n = 181). Participants received a comprehensive examination by trained calibrated dentists. Levels 36 unstimulated saliva samples were measured using Bio-Plex200 (Luminex). Salivary S. mutans C. quantified viable count (CFU/ml). Quantities colony-forming-units converted natural log data for statistical analysis. Latent class analysis was used clustering effect among participants. Results: The found high quantities pregnant varying detection rates. Univariate higher level IFN-g TNF-b < 4 decayed teeth, compared those ≥ teeth (p 0.05). Among more than 105 CFU/ml saliva, FLT-3L, IL-17a, TNF-B, VEGF-a significantly decreased 0.05), while G-CSF increased Lower VEGF-a, Eotaxin IL-9 individuals Two distinct clusters identified 181 participants, low-level high-level markers. logistic regression model Least Absolute Shrinkage Selection Operator (LASSO) penalty several potential variables Debiased results indicated statistically significant plaque these correlating lower In summary, our research underscores intricate relationship status, microbial populations, women.

Language: Английский

Citations

0