Bifidobacterium longum subsp. infantis B6MNI Alleviates Collagen-Induced Arthritis in Rats via Regulating 5-HIAA and Pim-1/JAK/STAT3 Inflammation Pathways DOI
Bowen Li, Mengfan Ding, Chi Chen

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(46), P. 17819 - 17832

Published: Oct. 31, 2023

The immunomodulatory potential of certain bacterial strains suggests that they could be beneficial in the treatment rheumatoid arthritis (RA). In this study, we investigated effects Bifidobacterium longum subsp. infantis B6MNI on progression collagen-induced (CIA) rats as well its influence gut microbiota and fecal metabolites. Forty-eight female Wistar were divided into six groups included a group with CIA intragastrically administered B. (109 CFU/day/rat), control (CON), group, both which intracardiacally same volume saline. Rats sacrificed after short-term (ST, 4 weeks) or long-term (LT, 6 administration. results indicate can modulate metabolites, including 5-hydroxyindole-3-acetic acid (5-HIAA), turn impacts expression Pim-1 immune cell differentiation, then through JAK-STAT3 pathway affects joint inflammation, regulates osteoclast differentiation factors, delays RA. Our also suggest is most efficacious for early middle stages

Language: Английский

Metabolic profiling and growth characteristics of a spaceflight-induced mutant of Lacticaseibacillus rhamnosus: Unveiling enhanced carbohydrate and amino acid metabolism for improved probiotic potential DOI
Shuai Guo, Yue Sun, Ting Wu

et al.

Food Bioscience, Journal Year: 2024, Volume and Issue: 58, P. 103758 - 103758

Published: Feb. 28, 2024

Language: Английский

Citations

3
Multi-omics insights into anti-colitis benefits of the synbiotic and postbiotic derived from wheat bran arabinoxylan and Limosilactobacillus reuteri DOI

Lanqi Zhou,

Song Wei, Tianqi Liu

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 278, P. 134860 - 134860

Published: Aug. 18, 2024

Language: Английский

Citations

3
Evaluation of In Vitro Production Capabilities of Indole Derivatives by Lactic Acid Bacteria DOI Creative Commons
B. Ma, Yan Zhao, Liping Liu

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(1), P. 150 - 150

Published: Jan. 13, 2025

Lactic acid Bacteria (LAB) convert tryptophan to indole derivatives and induce protective IL-22 production in vivo. However, differences metabolizing capabilities among LAB species have not been widely investigated. In the present study, we compared of 186 strains produce four kinds derivatives, including indole-3-carboxaldehyde (IAId), indole-3-lactic (ILA), indole-3-propanoic (IPA), indole-3-acetic (IAA). These were isolated from fermented foods, dairy products, feces healthy individuals, as well fish shrimp Shanxi Jiangsu provinces. They represent 15 genera, Bifidobacterium, Enterococcus, Lacticaseibacillus, Lactiplantibacillus, Lactobacillus, Lactococcus, Limosilactobacillus, Pediococcus, Streptococcus, Weissella, Latilactobacillus, Levilactobacillus, Ligilactobacillus, Loigolactobacillus. The results indicate widespread IAId-producing strains, with positive rates approximately 90% (106/117) 100% (69/69) provinces, respectively. concentrations IAId ranged 72.42 ng/mL 423.14 all Province 169.39 503.51 Province. Intriguingly, also observed specific ILA-producing Lactiplantibacillus 55.17% (16/29) 80.95% (17/21) overall detection ILA tested (including both other genus strains) 17.9% (21/117) 26.1% (18/69). 12.22 101.86 5.75 62.96 IPA IAA detected any strains. Finally, these derivative-producing related their geographical origins or isolation sources. current study provides insights into species- genus-dependent for derivatives. Defining roles derivative metabolism will uncover exact physiological mechanisms be helpful functional strain screening.

Language: Английский

Citations

0
Synergistical Amelioration of Muscle Atrophy by Modulating Gut Microbiota through the Bioconversion of Grape Seed Extract and Whey Using Kefir Lactic Acid Bacteria DOI Creative Commons
Kun‐Ho Seo,

Eseul Kim,

Hyunsook Kim

et al.

Future Foods, Journal Year: 2025, Volume and Issue: unknown, P. 100559 - 100559

Published: Feb. 1, 2025

Language: Английский

Citations

0
Specific dietary fiber combination modulates gut indole-3-aldehyde and indole-3-lactic acid levels to improve atopic dermatitis in mice DOI
Ning Wang, Zhangming Pei,

Hongchao Wang

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: 65, P. 106083 - 106083

Published: Feb. 6, 2025

Language: Английский

Citations

0
Bacillus megaterium GXU087 secretes indole - 3 - lactic acid to promote soybean growth and nodulation DOI Creative Commons

Jingsi Qiu,

Xiang‐Chen Meng, Jingdong Li

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: March 21, 2025

Bacillus species are recognized as plant growth-promoting bacteria (PGPB), yet the mechanisms behind their crop growth promotion remain elusive. This study is designed to explore (PGP) effects of megaterium GXU087 on soybeans and uncover underlying mechanism. In vitro , exhibited various PGP traits, including phosphate solubilization, nitrogen fixation, production exopolysaccharide, biofilm formation. Pot experiments indicated that significantly enhanced soybean growth, leading a remarkable increase in fresh weight ( p < 0.05). Additionally, nodulation parameters were improved. Specifically, 10% concentration extracellular extracts from exerted significant nodulation, with both nodule number increasing UPLC-MS analysis verified secreted indole-3-lactic acid (ILA) at 232.7 ng/mL. assays further demonstrated this ILA effectively promoted within range 0.1-10 mg/L. However, exogenous application did not stimulate rhizobia reproduction, suggesting bacterial does promote by enhancing growth. Notably, first report B. secreting promoter soybeans. Our findings offer new insights into mechanism action contribute understanding microbe-induced crops.

Language: Английский

Citations

0
The fermented cabbage metabolome and its protection against cytokine-induced intestinal barrier disruption of Caco-2 monolayers DOI Creative Commons
Lei Wei, Maria L. Marco

Applied and Environmental Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

ABSTRACT Fermented vegetables, such as fermented cabbage (sauerkraut), have garnered growing interest for their associations with a myriad of health benefits. However, the mechanistic details underlying outcomes consuming these foods require further investigation. This study examined capacity soluble metabolites in laboratory-scale and commercial-fermented to protect against disruption polarized Caco-2 monolayers by interferon gamma (IFN-γ) tumor necrosis factor-alpha (TNF-α). Laboratory-scale ferments (LSF) were prepared without addition Lactiplantibacillus plantarum NCIMB8826R (LP8826R) sampled after 7 14 days incubation. Trans-epithelial electrical resistance (TER) paracellular permeability fluorescein isothiocyanate (FITC)-dextran revealed that cabbage, but not raw or brine, protected cytokine-induced damage monolayers. Barrier-protective effects occurred despite increased IL-8 production following cytokine exposure. Metabolomic analyses performed using gas liquid chromatography resulted identification 149 333 metabolites, respectively. Significant differences found between cabbage. LSF metabolomes changed over time, profiles LP8826R best resembled commercial product. Overall, fermentation lower carbohydrate lactic acid, lipid, amino acid derivative (including D-phenyl-lactate [D-PLA], indole-3-lactate [ILA], γ-aminobutyric [GABA]), phenolic compound concentrations. Lactate, D-PLA, ILA tested individually combined only partially TER reductions increases The findings show intestinal barrier-protective compounds are consistently enriched during fermentations, irrespective scale microbial additions, which may contribute health-promoting potential foods. IMPORTANCE vegetables increasingly associated importance transformations process remains be determined. We spontaneously epithelial cells induced proinflammatory cytokines. Cabbage fermentations consistent metabolome bioactive known made beneficial members human gut microbiome, including (D-PLA) (ILA). distinct from differentiated lab ferments, sampling presence an exogenous strain. Because partial protection barrier was when individual (D-PLA, ILA, lactate) applied, indicate complex mixture offers advantages single benefit health.

Language: Английский

Citations

0
Novel cross-feeding human gut microbes metabolizing tryptophan to indole-3-propionate DOI Creative Commons
Janina N. Zünd,

Denisa Mujezinovic,

Markus Reichlin

et al.

Gut Microbes, Journal Year: 2025, Volume and Issue: 17(1)

Published: May 7, 2025

Tryptophan-derived indoles produced by the gut microbiota, particularly indole-3-propionate (IPA), are key compounds associated with gastrointestinal balance and overall health. Reduced levels of IPA have been inflammatory bowel disease, type 2 diabetes, colorectal cancer. Since fiber-rich diets shown to promote IPA, we aimed decipher fiber-specific effects identify IPA-producing taxa in a range healthy individuals. We cultured fecal microbiota from 16 adults tryptophan eight different dietary fibers monitored community shifts 16S rRNA gene amplicon sequencing tryptophan-derived using targeted liquid chromatography diode array detection. The concentrations types were donor-specific, pectin strongly promoting production certain donors. was not any known producer but pectin-utilizing species Lachnospira eligens, which indole-3-lactate (ILA) vitro, precursor. Supplementation ILA additional cultures (n = 6) revealed its effective use as substrate for production. identified novel producer, Enterocloster aldenensis, exclusively tryptophan. Co-culture L. eligens E. aldenensis resulted production, providing new evidence an cross-feeding mechanism that may contribute IPA-promoting observed pectin. Overall, highlight potential interventions beneficial metabolites.

Language: Английский

Citations

0
What We Know About the Actual Role of Traditional Probiotics in Health and Disease DOI
Faisal Al‐Akayleh, Ahmed S.A. Ali Agha, Mayyas Al‐Remawi

et al.

Probiotics and Antimicrobial Proteins, Journal Year: 2024, Volume and Issue: 16(5), P. 1836 - 1856

Published: May 3, 2024

Language: Английский

Citations

3
A diet-dependent host metabolite shapes the gut microbiota to protect from autoimmunity DOI Open Access
Margaret Alexander, Vaibhav Upadhyay,

Rachel Rock

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 3, 2023

Diet can protect from autoimmune disease; however, whether diet acts via the host and/or microbiome remains unclear. Here, we use a ketogenic (KD) as model to dissect these complex interactions. A KD rescued experimental encephalomyelitis (EAE) mouse of multiple sclerosis in microbiota-dependent fashion. Dietary supplementation with single KD-dependent metabolite (β-hydroxybutyrate, βHB) EAE whereas transgenic mice unable produce βHB intestine developed more severe disease. Transplantation βHB-shaped gut microbiota was protective.

Language: Английский

Citations

5