Behavior, hormone, and gut microbiota change by YYNS intervention in an OVX mouse model DOI Creative Commons

Huajuan Lei,

Jian Liu, Juan Deng

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Nov. 7, 2024

Object Perimenopause depression disorder (PDD) is a very common problem in clinical practice and characterized by autonomic nervous symptoms, including hot flashes, palpitation, night sweating. In addition, the comorbidity of menopause has long been an integral component estradiol (E2) shortage. Previous studies have suggested that mechanisms underlying this involved overlap endocrine cerebellar networks. Emerging evidence shown endocrine–brain–gut–microbiota axis plays key role regulation affective disorders. Yangyin-ningshen formula (YYNS) traditional Chinese decoction tailored Yijintang for menopausal intervention. Thus, we hypothesized YYNS may be alleviation through axis. Methods To verify this, constructed bilateral ovariectomy (OVX) mouse model to simulate menopausal-related depression. Subsequently, behavioral tests open field test (OFT) forced swimming (FST) were conducted examine state post-OVX. With or E2 intervention, enzyme-linked immunosorbent assay (ELISA) was used determine serum sex hormones level. 16S rRNA gene sequencing liquid chromatography-mass spectrometry (LC-MS) analyze microbiome colon samples collected from mice sham surgery group (CSH), OVX (CMD), with hormone intervention (CHM), (CYYNS). One week after OVX, CMD, CHM, CYYNS showed OFT, FST. Three weeks post-OVX, CHM notable relief depression; CMD shaped OTUs shrinkage; raised sham, group. The abundance Actinobiota decreased but Bacteriodia increased. relative genus varied each Moreover, functional correlation changes gut microbes between different groups PRL level negatively correlated Odoribacter . T positively Lachnospiraceae NK4A136 ( p < 0.05). Conclusion Our results not only offer novel insights into also build important basis therapeutic efficacy on PDD, which provide future research etiology network.

Language: Английский

Specific gut microbiota and serum metabolite changes in patients with osteoarthritis DOI Creative Commons
Wendong Wang, Xincheng Liu, Nan Hao

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: Feb. 14, 2025

Introduction Recent research indicated a strong link between the gut microbiota and osteoarthritis. However, complex interplay microbiota, serum metabolites, progression of osteoarthritis in affected individuals remains largely unexplored. This study aimed to investigate characteristics metabolites patients with Methods Participants either healthy knees or were enrolled categorized into control (HC) (OA) groups. Fecal blood samples collected for 16S rRNA gene sequencing, metabolomic analysis via liquid chromatography–mass spectrometry (LC-MS), integrated evaluation. Results The results showed no significant variation richness diversity two abundance Bacteroides plebeius Faecalibacterium prausnitzii was reduced OA group, both which are known their potential as next-generation probiotics human health. Metabolomic that including pyrogallol 3-hydroxybutyrate (3HB), significantly lower group. These positively impact other diseases demonstrated good diagnostic performance distinguishing from controls. Correlation revealed positive correlation 3HB. Discussion highlighted specific metabolite profiles patients, suggesting changes bacteria derived closely tied progression. underscores modifiable elements therapeutic targets prevention.

Language: Английский

Citations

0
Cross-talks between osteoporosis and gut microbiome DOI
Shiva Shankar Jha, Naveen Jeyaraman,

Madhan Jeyaraman

et al.

World Journal of Orthopedics, Journal Year: 2025, Volume and Issue: 16(3)

Published: March 12, 2025

Language: Английский

Citations

0
[Moxibustion Inhibits Postmenopausal Bone Loss by Regulating the Metabolism of Gut Microbiota-Related Serotonin]. DOI

Xianhao Huang,

P. Alberto,

Huimin Xu

et al.

PubMed, Journal Year: 2025, Volume and Issue: 56(1), P. 129 - 136

Published: Jan. 20, 2025

To investigate the regulatory effect of moxibustion, a traditional Chinese medicine therapy, on bone metabolism in ovariectomized (estrogen-deficient) mice and to explore its underlying mechanisms. Female C57BL/6J 12 weeks old were randomly assigned five groups, including sham operation control group (SHAM), an ovariectomy (OVX), given broad-spectrum antibiotics (OVX-A), moxibustion (OVX-M), ovariectomy, antibiotics, (OVX-A-M), with 5 each group. Then, 4 post-surgery, received and/or intervention for additional weeks. After that, sacrificed, samples collected. Micro-CT was used assess volume parameters distal femurs, volume/tissue fraction (BV/TV), trabecular number (Tb.N), thickness (Tb.Th), separation (Tb.Sp), cortical (Ct.Th). Targeted metabolomics measure serum tryptophan metabolites, qPCR performed quantify serotonin (SER) receptors Htr2a Htr2b mRNA levels marrow. In addition, primary marrow mesenchymal stem cells (BMSCs) treated varying concentration gradients (0, 0.01, 0.1, 1, 10 μmol/L). Alkaline phosphatase (ALP) staining osteogenic differentiation, while expression Colla1, osteogenesis-related gene, Htr2b. Compared SHAM mice, OVX exhibited significant deterioration microarchitecture, showing decreased BV/TV ([10.57 ± 2.82]% vs. [4.20 0.96]%, P < 0.01), reduced Tb.N ([3.16 0.11] [2.25 0.15], increased Tb.Sp ([0.31 0.01] [0.45 0.03], metabolite SER. OVX-M showed notable improvement increasing (7.51 1.42)% (P 0.05), elevated SER gene 0.05). However, reversing loss disappeared when gut microbiota disrupted by antibiotics. OVX-A-M had significantly lower compared 0.001). According findings from vitro experiments, enhanced differentiation BMSCs, optimal achieved at 0.1 μmol/L. Furthermore, μmol/L genes Colla1 Moxibustion therapy can inhibit postmenopausal loss, potentially regulating microbiota-derived SER, activating 5-HT2A receptor, promoting BMSCs.

Language: Английский

Citations

0
Integrated metabolomics and gut microbiota analysis to explore the protective effects of Gushudan on postmenopausal osteoporosis rats via gut-bone axis DOI
Qinghua Liang, Hongying Du, Yajing Wang

et al.

Journal of Pharmaceutical and Biomedical Analysis, Journal Year: 2025, Volume and Issue: 263, P. 116942 - 116942

Published: May 3, 2025

Language: Английский

Citations

0
Progress of research on the gut microbiome and its metabolite short-chain fatty acids in postmenopausal osteoporosis: a literature review DOI
Qianming Chen, Ying Xie, Xijie Yu

et al.

Frontiers of Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: May 10, 2025

Language: Английский

Citations

0
Effects of probiotic supplementation on bone health in postmenopausal women: a systematic review and meta-analysis DOI Creative Commons
Fang Wang, Wei Wei, Peng Ju Liu

et al.

Frontiers in Endocrinology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 1, 2024

The beneficial effects of probiotic supplementation on bone health in postmenopausal women require further validation.

Language: Английский

Citations

2
Behavior, hormone, and gut microbiota change by YYNS intervention in an OVX mouse model DOI Creative Commons

Huajuan Lei,

Jian Liu, Juan Deng

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Nov. 7, 2024

Object Perimenopause depression disorder (PDD) is a very common problem in clinical practice and characterized by autonomic nervous symptoms, including hot flashes, palpitation, night sweating. In addition, the comorbidity of menopause has long been an integral component estradiol (E2) shortage. Previous studies have suggested that mechanisms underlying this involved overlap endocrine cerebellar networks. Emerging evidence shown endocrine–brain–gut–microbiota axis plays key role regulation affective disorders. Yangyin-ningshen formula (YYNS) traditional Chinese decoction tailored Yijintang for menopausal intervention. Thus, we hypothesized YYNS may be alleviation through axis. Methods To verify this, constructed bilateral ovariectomy (OVX) mouse model to simulate menopausal-related depression. Subsequently, behavioral tests open field test (OFT) forced swimming (FST) were conducted examine state post-OVX. With or E2 intervention, enzyme-linked immunosorbent assay (ELISA) was used determine serum sex hormones level. 16S rRNA gene sequencing liquid chromatography-mass spectrometry (LC-MS) analyze microbiome colon samples collected from mice sham surgery group (CSH), OVX (CMD), with hormone intervention (CHM), (CYYNS). One week after OVX, CMD, CHM, CYYNS showed OFT, FST. Three weeks post-OVX, CHM notable relief depression; CMD shaped OTUs shrinkage; raised sham, group. The abundance Actinobiota decreased but Bacteriodia increased. relative genus varied each Moreover, functional correlation changes gut microbes between different groups PRL level negatively correlated Odoribacter . T positively Lachnospiraceae NK4A136 ( p &lt; 0.05). Conclusion Our results not only offer novel insights into also build important basis therapeutic efficacy on PDD, which provide future research etiology network.

Language: Английский

Citations

1