Diagnostic utility of SARS-CoV-2 nucleocapsid antigenemia: a meta-analysis DOI Creative Commons
Gregory L. Damhorst,

Sydney E Martin,

Eli Wilber

et al.

Open Forum Infectious Diseases, Journal Year: 2024, Volume and Issue: 11(10)

Published: Sept. 27, 2024

Abstract Background Studies of the diagnostic performance severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid antigen in blood (antigenemia) have reached variable conclusions. The potential utility antigenemia measurements as a clinical test needs clarification. Methods We performed systematic review Pubmed, Embase, and Scopus through July 15, 2023, requested source data from corresponding authors. Results Summary sensitivity 16 studies (4543 cases) sampled at ≤14 days symptoms was 0.83 (0.75–0.89), specificity 0.98 (0.87–1.00) 6 (792 reverse transcription polymerase chain reaction–negative controls). for paired specimens with cycle threshold values ≤33 were 0.91 (0.85–0.95) 0.56 (0.39–0.73) 10 (612 individuals). Source 1779 cases reveal that >70% weeks following symptom onset, which persists <10% 28 days. available suffer heterogeneity, Omicron-era are scarce. Conclusions Nucleocapsid currently has limited due to limitations existing lack data. Improved study designs targeting uses screening, surveillance, complex decision-making—especially immunocompromised patients—are needed.

Language: Английский

Unraveling the role of the nucleocapsid protein in SARS-CoV-2 pathogenesis: From viral life cycle to vaccine development DOI
Yousra A. El‐Maradny,

Moustafa A. Badawy,

Kareem I. Mohamed

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 279, P. 135201 - 135201

Published: Aug. 30, 2024

Language: Английский

Citations

6
Isolation and molecular characterization of an enteric isolate of the Genotype-Ia Bovine coronavirus with notable mutations in the receptor binding domain of the spike glycoprotein. DOI Creative Commons

Abid Ullah Shah,

Phillip C. Gauger, Maged Gomaa Hemida

et al.

Virology, Journal Year: 2024, Volume and Issue: 603, P. 110313 - 110313

Published: Nov. 29, 2024

Language: Английский

Citations

4
Distinct roles of SARS-CoV-2 N protein and NFP in host cell response modulation DOI

Hsin-Chi Lan,

Baidong Hou,

Shu-Ting Chang

et al.

Journal of Molecular Biology, Journal Year: 2025, Volume and Issue: unknown, P. 169094 - 169094

Published: March 1, 2025

Language: Английский

Citations

0
Research Progress on the Structure and Function, Immune Escape Mechanism, Antiviral Drug Development Methods, and Clinical Use of SARS-CoV-2 Mpro DOI Creative Commons
Jiayi Ren, Zhengfu Zhang, Yi Xia

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(2), P. 351 - 351

Published: Jan. 16, 2025

The three-year COVID-19 pandemic ‘has’ caused a wide range of medical, social, political, and financial implications. Since the end 2020, various mutations variations in SARS-CoV-2 strains, along with immune escape phenomenon, have emerged. There is an urgent need to identify relatively stable target for development universal vaccines drugs that can effectively combat both strains their mutants. Currently, main focus treating lies disrupting virus’s life cycle. protease (Mpro) closely associated virus replication maturation plays crucial role early stages infection. Consequently, it has become important SARS-CoV-2-specific drugs. This review summarizes recent research progress on novel coronavirus’s proteases, including pivotal Mpro cycle, structure catalytic mechanism Mpro, self-maturation escape, current methods developing antiviral targeting key successfully entered clinical trials. aim provide researchers involved systematic comprehensive information.

Language: Английский

Citations

0
Isolation and molecular characterization of an enteric isolate of the Genotype-I Bovine coronavirus with notable mutations in the receptor binding domain of the spike glycoprotein and deletion downstream the RNA binding domain of the nucleocapsid protein DOI Open Access

Abid Ullah Shah,

Phillip C. Gauger, Maged Gomaa Hemida

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 4, 2024

Abstract Bovine coronavirus (BCoV) continues to be a significant threat cattle populations despite the implementation of vaccination programs. The continuous circulation BCoV highlights necessity for ongoing genomic surveillance understand better virus’s evolution and its impact on health. main goal this study was do isolation perform comprehensive molecular characterization new enteric field isolate BCoV. To identify any genetic elements in sequences that could act as markers infection cattle. achieve these objectives, newly identified propagated MDBK cell line several subsequent blind passages. immunofluorescence assay verified confirmation virus propagation. We plaque purified titrated it by using HRT-18 line. examined viral protein expression SDS-PAGE followed Western blot BCoV/S BCoV/N antibodies. Our results show substantial increase genome copy number, expression, infectivity with culture full-length sequence NGS drafted. vial is 31 Kb length. has typical organization (5’-UTR-Gene- 1- HE-S-M-E-N-UTR-3’). phylogenetic analysis based nucleotide (full-length genome, S, HE, N) showed BCoV-13 clustered other members (genotype I-i). shows synonymous mutations among various domains S glycoprotein, especially receptor binding domain. found nine notable deletions immediately downstream RNA domain nucleocapsid gene. Further gene function studies are encouraged pathogenesis immune regulation/evasion. This research enhances our understanding genomics contributes improved diagnostic control measures infections populations.

Language: Английский

Citations

0
Structural basis for the participation of the SARS-CoV-2 nucleocapsid protein in the template switch mechanism and genomic RNA reorganization DOI Creative Commons
Peter Reis Bezerra, Fábio C. L. Almeida

Journal of Biological Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 107834 - 107834

Published: Sept. 1, 2024

Language: Английский

Citations

0
Diagnostic utility of SARS-CoV-2 nucleocapsid antigenemia: a meta-analysis DOI Creative Commons
Gregory L. Damhorst,

Sydney E Martin,

Eli Wilber

et al.

Open Forum Infectious Diseases, Journal Year: 2024, Volume and Issue: 11(10)

Published: Sept. 27, 2024

Abstract Background Studies of the diagnostic performance severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid antigen in blood (antigenemia) have reached variable conclusions. The potential utility antigenemia measurements as a clinical test needs clarification. Methods We performed systematic review Pubmed, Embase, and Scopus through July 15, 2023, requested source data from corresponding authors. Results Summary sensitivity 16 studies (4543 cases) sampled at ≤14 days symptoms was 0.83 (0.75–0.89), specificity 0.98 (0.87–1.00) 6 (792 reverse transcription polymerase chain reaction–negative controls). for paired specimens with cycle threshold values ≤33 were 0.91 (0.85–0.95) 0.56 (0.39–0.73) 10 (612 individuals). Source 1779 cases reveal that >70% weeks following symptom onset, which persists <10% 28 days. available suffer heterogeneity, Omicron-era are scarce. Conclusions Nucleocapsid currently has limited due to limitations existing lack data. Improved study designs targeting uses screening, surveillance, complex decision-making—especially immunocompromised patients—are needed.

Language: Английский

Citations

0