Akkermansia muciniphila Protects Against Trinitrobenzene Sulfonic Acid Induced Colitis by Inhibiting IL6/STAT3 Pathway DOI
Mingshan Jiang,

Yongbin Jia,

Chunxiang Ma

et al.

Inflammatory Bowel Diseases, Journal Year: 2025, Volume and Issue: unknown

Published: April 10, 2025

Abstract Background Inflammatory bowel disease is a long-standing inflammatory disorder that influences the intestinal tract. The intent of this research to explore whether relative abundance Akkermansia muciniphila related IL6/STAT3 pathway and fundamental molecular mechanisms A. on trinitrobenzene sulfonic acid (TNBS)-induced enteritis mouse model, including expression cytokines proteins in signaling pathway. Methods association between was investigated by using mucosal biopsies fecal samples. TNBS-induced colitis models were performed elucidate underlying mechanisms. alteration microbiota organized 16s rRNA sequencing. Results In Crohn’s patients, level STAT3 IL-6 presented negative relationship with muciniphila. IL-6, p-STAT3, downregulated A.m+TNBS group, indicating may inhibit vivo. To investigate potential defensive role supplementation vivo enteritis, 16S sequencing analyze changes composition. results revealed marked increase microbial diversity within muciniphila-treated suggesting beneficial modulation gut microbiome associated supplementation. Conclusions Our findings declared alleviates gastrointestinal inflammation through IL-6/STAT3 This protective effect mediated downregulation STAT3, highlighting mechanism which modulates responses. work disclosed demonstrates influence against vivo, proposing it qualified as unique therapeutic focusing modulating or p-STAT3 treatment colitis.

Language: Английский

Epstein-Barr virus Infection Exacerbates Ulcerative Colitis by Driving Macrophage Pyroptosis via the Upregulation of Glycolysis DOI Creative Commons
Chunxiang Ma, Kexin Chen, Lili Li

et al.

Precision Clinical Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 21, 2025

Epstein-Barr virus (EBV) infection is associated with clinical symptoms, treatment response, need for surgical intervention, and an enhanced likelihood of lymphoma among patients ulcerative colitis (UC). However, existing studies have primarily concentrated on the epidemiological associations between EBV UC, leaving mechanisms by which exacerbates poorly understood. Clinical specimens UC a mouse model dextran sulfate sodium-induced concurrent murine γ-herpesvirus 68 (MHV-68) were utilized to investigate relationship macrophage pyroptosis. In vivo, adoptive transfer MHV-68-induced macrophages depletion performed elucidate underlying mechanisms. vitro, myeloid leukemia mononuclear cells human (THP-1) derived from bone marrow (BMDMs) stimulated MHV-68, respectively, assess pyroptosis glycolysis. EBV-induced activation was positively correlated disease activity in patients. Furthermore, MHV-68 activated upregulating gasdermin D, NLRP3, interleukin-1β, interleukin-18 colonic tissues peritoneal mice colitis. also mediated THP-1 BMDMs, respectively. Additionally, BMDMs aggravated colitis, whereas attenuated intestinal injury. Mechanistically, promoted glycolysis, while glycolysis inhibitor, 2-deoxy-D-glucose, blocked this process vitro. driving through upregulation indicating potential therapeutic approach mitigate inflammation.

Language: Английский

Citations

2
Gut virome and its implications in the pathogenesis and therapeutics of inflammatory bowel disease DOI Creative Commons

Yushan Wu,

Rui Cheng, Hao Lin

et al.

BMC Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: March 26, 2025

Language: Английский

Citations

0
Akkermansia muciniphila Protects Against Trinitrobenzene Sulfonic Acid Induced Colitis by Inhibiting IL6/STAT3 Pathway DOI
Mingshan Jiang,

Yongbin Jia,

Chunxiang Ma

et al.

Inflammatory Bowel Diseases, Journal Year: 2025, Volume and Issue: unknown

Published: April 10, 2025

Abstract Background Inflammatory bowel disease is a long-standing inflammatory disorder that influences the intestinal tract. The intent of this research to explore whether relative abundance Akkermansia muciniphila related IL6/STAT3 pathway and fundamental molecular mechanisms A. on trinitrobenzene sulfonic acid (TNBS)-induced enteritis mouse model, including expression cytokines proteins in signaling pathway. Methods association between was investigated by using mucosal biopsies fecal samples. TNBS-induced colitis models were performed elucidate underlying mechanisms. alteration microbiota organized 16s rRNA sequencing. Results In Crohn’s patients, level STAT3 IL-6 presented negative relationship with muciniphila. IL-6, p-STAT3, downregulated A.m+TNBS group, indicating may inhibit vivo. To investigate potential defensive role supplementation vivo enteritis, 16S sequencing analyze changes composition. results revealed marked increase microbial diversity within muciniphila-treated suggesting beneficial modulation gut microbiome associated supplementation. Conclusions Our findings declared alleviates gastrointestinal inflammation through IL-6/STAT3 This protective effect mediated downregulation STAT3, highlighting mechanism which modulates responses. work disclosed demonstrates influence against vivo, proposing it qualified as unique therapeutic focusing modulating or p-STAT3 treatment colitis.

Language: Английский

Citations

0