Comprehensive Engineering Strategies for Heterologous Production of Zealexin A1 in Saccharomyces cerevisiae DOI
Yukun Li, Ran Li, Jianjun Ge

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(34), P. 19071 - 19080

Published: Aug. 14, 2024

Zealexin A1 is a nonvolatile sesquiterpene phytoalexin, which not only exhibits extensive antifungal and insecticidal activities but also has the ability to enhance drought resistance of plants, thus potential applications in agricultural food fields. In this study, biosynthetic pathway zealexin was constructed

Language: Английский

The E factor at 30: a passion for pollution prevention DOI Creative Commons
Roger A. Sheldon

Green Chemistry, Journal Year: 2023, Volume and Issue: 25(5), P. 1704 - 1728

Published: Jan. 1, 2023

Publication of the E Factor drew attention to problem waste in chemicals manufacture. Thirty yeas later it is abundantly clear that underlying cause global environmental problems, from climate change plastic pollution.

Language: Английский

Citations

148
Metabolic and enzymatic engineering strategies for polyethylene terephthalate degradation and valorization DOI Creative Commons
Alessandro Satta, Guido Zampieri, Giovanni Loprete

et al.

Reviews in Environmental Science and Bio/Technology, Journal Year: 2024, Volume and Issue: 23(2), P. 351 - 383

Published: May 14, 2024

Abstract Polyethylene terephthalate (PET) is one of the most marketed aromatic polyesters in world with an annual demand 2022 approximately 29 million metric tons, expected to increase by 40% 2030. The escalating volume PET waste and current inadequacy recycling methods have led accumulation terrestrial ecosystem, thereby posing significant global health risks. pressing energy environmental issues associated underscore urgent need for “upcycling” technologies. These technologies aim transform reclaimed into higher-value products, addressing both concerns sustainability. Enzyme-mediated biocatalytic depolymerization has emerged as a potentially bio-sustainable method treating plastics. Numerous plastic-degrading enzymes been identified from microbial origins, advancements protein engineering employed modify enhance these enzymes. Microbial metabolic allows development modified chassis capable degrading substrates converting their derived monomers industrial relevant products. In this review, we describe several approaches aiming at enhancing performances PET-degrading present strategies adopted bio-upcycle high-value molecules.

Language: Английский

Citations

12
Design of fusion proteins for biocatalysis DOI
Beyzanur Celebi,

Janina Lawniczek,

David Angelo V Guanzon

et al.

Methods in enzymology on CD-ROM/Methods in enzymology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

1
Rational Design of Spontaneous Self-Cyclization Enzymes In Vivo and In Vitro with Improved Thermal Tolerance and Activity DOI
Bin Wei, Yilin Kang, Yuxuan Zhao

et al.

ACS Catalysis, Journal Year: 2024, Volume and Issue: 14(7), P. 5469 - 5480

Published: March 28, 2024

Enzymes have selectivity, require mild catalytic conditions, and are important cornerstones in many industrial processes. Protein self-cyclization has opened up the possibility of preserving fragile enzymes during long-term high-temperature catalysis. However, mechanism for improvement thermal tolerance not been elucidated, severely limiting their applications. Herein, we provide a strategy rational design fusion proteins based on structural analysis to obtain cyclized with improved properties. First, constructed that preferentially translated SpyCatcher (CBT) or SpyTag (TBC), both which could form stable single significantly tolerance. Especially, half-life TBC obtained by modifying N-terminal at 40 °C was 10.83 times wild enzymes. Structural revealed terminus protein, SpyCatcher, folded into conformation adversely affected stability. In addition, structure pocket orientation residues CBT were different from those wild-type enzymes, led decrease activity. These conclusions confirmed when another enzyme, sucrose phosphorylase, cyclized. Finally, glucosidase also superior strain preparation ginsenoside F1 high titers as whole-cell catalyst extended use. conclusion, demonstrated first time conjugated long oligopeptide activity stability It necessary translate units less steric hindrance reduce impact protein structure. The provides simple effective modification poor tolerance, providing considerable prospects biosynthesis vivo vitro.

Language: Английский

Citations

7
The Role of Microbial Diversity in Lignocellulosic Biomass Degradation: A Biotechnological Perspective DOI
Ghulam Rasool, Muhammad Irfan

ChemBioEng Reviews, Journal Year: 2024, Volume and Issue: 11(3), P. 613 - 635

Published: April 29, 2024

Abstract Lignocellulosic biomass, such as plant residues and agricultural waste, holds immense potential a renewable resource for the production of biofuels, chemicals, animal feed. However, efficient degradation lignocellulose into fermentable sugars remains significant challenge. Recent research has highlighted critical role microbial diversity in lignocellulosic biomass degradation, offering new insights from biotechnological perspective. The comprehension utilization are crucial developing strategies degradation. By uncovering intricate relationships between communities their enzymatic machinery, researchers can optimize processes, enhance biofuel production, contribute to more sustainable bio‐based economy. Microorganisms, including bacteria, fungi, archaea, possess diverse capabilities, allowing them secrete plethora lignocellulolytic enzymes. Microbial organisms inhabiting extreme environments, rumen, hot cold springs, deep sea trenches, acidic alkaline pH exhibit generating enzymes, hemicellulolytic which superior biochemical properties essential industrial bioconversion applications. This review explores ability enzymes sources efficiently break down applications biotechnology.

Language: Английский

Citations

5
Engineering of 4-hydroxyphenylacetate 3-hydroxylase derived from Pseudomonas aeruginosa for the ortho-hydroxylation of ferulic acid DOI

Ping Sun,

Pu Zheng, Pengcheng Chen

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 264, P. 130545 - 130545

Published: Feb. 29, 2024

Language: Английский

Citations

4
Enhanced degradation of post-consumer polyethylene terephthalate (PET) wastes by fusion cutinase: Effects of anchor and linker peptides DOI

Qingjun Jia,

Zixuan Zhang,

Lankai Su

et al.

Process Biochemistry, Journal Year: 2024, Volume and Issue: 145, P. 1 - 12

Published: June 17, 2024

Language: Английский

Citations

4
Advances in microbial production of geraniol: from metabolic engineering to potential industrial applications DOI
Xun Wang, Xinyi Zhang, Jia Zhang

et al.

Critical Reviews in Biotechnology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 16

Published: Sept. 12, 2024

Geraniol, an acyclic monoterpene alcohol, has significant potential applications in various fields, including: food, cosmetics, biofuels, and pharmaceuticals. However, the current sources of geraniol mainly include plant tissue extraction or chemical synthesis, which are unsustainable suffer severely from high energy consumption severe environmental problems. The process microbial production recently undergone vigorous development. Particularly, sustainable construction recombinant

Language: Английский

Citations

4
Chimeric enzymes in the pulp and paper making industry: Current developments DOI
Abdullah Arsalan,

Yuvaraj Ravikumar,

Xinrui Tang

et al.

Biotechnology Advances, Journal Year: 2025, Volume and Issue: unknown, P. 108530 - 108530

Published: Feb. 1, 2025

Language: Английский

Citations

0
Functional and structural insights into α-L-Rhamnosidase: cloning, characterization, and decoding evolutionary constraints through structural motif DOI

Yupeng Liang,

Yalan Zhao,

Zhongwei Yin

et al.

Archives of Microbiology, Journal Year: 2025, Volume and Issue: 207(3)

Published: Feb. 15, 2025

Language: Английский

Citations

0