Considerations for cultivated crustacean meat: potential cell sources, potential differentiation and immortalization strategies, and lessons from crustacean and other animal models

Critical Reviews in Food Science and Nutrition, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 25

Published: May 11, 2024

Cultivated crustacean meat (CCM) is a means to create highly valued shrimp, lobster, and crab products directly from stem cells, thus removing the need farm or fish live animals. Conventional enterprises face increasing pressures in managing overfishing, pollution, warming climate, so CCM may provide way ensure sufficient supply as global demand for these grows. To support development of CCM, this review briefly details cell culture work date, before addressing what presently known about muscle development, particularly molecular mechanisms involved, how might relate recent on cultivated production vertebrate species. Recognizing current lack lines available establish cultures, we also consider primary sources that can be obtained non-lethally including tissues limbs which are readily released regrown, putative cells circulating hemolymph. Molecular approaches inducing myogenic differentiation immortalization reviewed. Finally, assess status tools researchers, antibodies, propose avenues address existing shortfalls order see field progress.

Language: Английский

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The Role of Cholesterol during the Ovarian Maturation and Lipid Metabolism of Female Chinese Mitten Crab (Eriocheir sinensis)

Aquaculture Nutrition, Journal Year: 2024, Volume and Issue: 2024(1)

Published: Jan. 1, 2024

In previous study, we found that the cholesterol requirement of Eriocheir sinensis was 0.27%, to further investigate effects on health status, ovarian maturation, and lipid metabolism female broodstock. Two diets containing 0% 0.25% (actually 0.05% 0.27%) were fed crabs (average weight: 49.21 ± 0.11 g) for 4 months sampled once a month. The results showed body weight (BW), survival rate (SR), meat yield (MY), condition factor (CF), hepatosomatic index (HSI), gonadosomatic (GSI) significantly affected by treatment time compared with deficient group, supplementing increased BW, HSI, GSI ( P < 0.05). addition, long‐term lack will lead significant decrease in activity ACP, AKP, SOD increase content MDA. histological volume oocytes Further studies 0.27% transcription levels vtg vgr hepatopancreas ovaries, which may be main reason oocyte size When diet, contents nutrients ovaries significantly, especially lipids Through analysis mRNA expression level genes related metabolism, it enhanced synthesis transport hepatopancreas, thereby promoting accumulation organism. Furthermore, control juvenile hormone (JH), 17 β ‐estradiol (E 2 ), methyl farnesoate (MF), ecdysone organism after feeding diet summary, an appropriate amount can improve growth performance broodstock, enhance body’s antioxidant immune system, promote maturation.

Language: Английский

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The moulting arthropod: a complete genetic toolkit review

Biological reviews/Biological reviews of the Cambridge Philosophical Society, Journal Year: 2024, Volume and Issue: 99(6), P. 2338 - 2375

Published: July 22, 2024

ABSTRACT Exoskeletons are a defining character of all arthropods that provide physical support for their segmented bodies and appendages as well protection from the environment predation. This ubiquitous yet evolutionarily variable feature has been instrumental in facilitating adoption variety lifestyles exploitation ecological niches across environments. Throughout radiation produced more than one million described modern species, adaptability afforded by segmentation exoskeletons led to diversity is unrivalled amongst animals. However, because limited extensibility exoskeleton chitin cuticle components, they must be periodically shed replaced with new larger ones, notably accommodate growing individuals encased within. Therefore, grow discontinuously undergoing periodic moulting events, which follow series steps preparatory pre‐moult phase ecdysis itself post‐moult maturation exoskeletons. Each event represents particularly vulnerable period an arthropod's life cycle, so processes tightly regulated meticulously executed ensure successful transitions normal growth development. Decades research representative foundation understanding mechanisms involved. Building on this, studies continue develop test hypotheses presence function molecular including neuropeptides, hormones, receptors, so‐called early, late, fate genes, arthropod diversity. Here, we review literature comprehensive overview status accumulated knowledge genetic toolkit governing moulting. From biosynthesis regulation ecdysteroid sesquiterpenoid factors involved hormonal stimulation responses remodelling, identify commonalities differences, highlighting major gaps, groups. We examine available evidence supporting current models how components operate together prepare for, execute, recover ecdysis, comparing reports Chelicerata, Myriapoda, Crustacea, Hexapoda. Evidence generally highly taxonomically imbalanced, most based insect study systems. Biases also evident different phases processes, early triggers late effectors being least explored. Our synthesis contrasts reported observations reasonably plausible assumptions given taxonomic sampling, exposes weak or gaps need addressing. Encouragingly, advances genomics driving diversification tractable systems cataloguing putative toolkits previously under‐explored taxa. Analysis genome transcriptome data supported experimental investigations have validated “ultra‐conserved” core genes processes. The machinery likely evolved elaborations this conserved pathway backbone, but exploration needed characterise lineage‐specific changes novelties. Furthermore, linking these transformative innovations Arthropoda remains hampered untested assumptions. Promisingly however, emerging framework highlights avenues underlying genetics dynamic biology through complex physiology

Language: Английский

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A brief and updated introduction to the neuroendocrine system of crustaceans

Molecular and Cellular Endocrinology, Journal Year: 2024, Volume and Issue: 590, P. 112265 - 112265

Published: May 1, 2024

Language: Английский

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Transcriptomic Analysis across Crayfish (Cherax quadricarinatus) Claw Regeneration Reveals Potential Stem Cell Sources for Cultivated Crustacean Meat

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 8623 - 8623

Published: Aug. 7, 2024

In the face of rising global demand and unsustainable production methods, cultivated crustacean meat (CCM) is proposed as an alternative means to produce delicious lobster, shrimp, crab products. Cultivated requires starting stem cells that may vary in terms potency propensity proliferate or differentiate into myogenic (muscle-related) tissues. Recognizing regenerating limbs are a non-lethal source tissue harbor relevant cells, we selected those crayfish Cherax quadricarinatus our model. To investigate cell activity, conducted RNA-Seq analysis across six stages claw regeneration (four pre-molt two post-molt stages), along with histology real-time quantitative PCR (qPCR). Our results showed while genes related energy production, muscle hypertrophy, exoskeletal cuticle synthesis dominated stages, growth factor receptors (FGFR, EGFR, TGFR, BMPR) proliferation (Cyclins, CDKs, Wnts, C-Myc, Klf4, Sox2, PCNA, p53) were upregulated before molt. Pre-molt upregulation several occurred peaks; Stages 2 4. We therefore propose limb tissues, particularly larger Stage 4, present prolific for CCM development.

Language: Английский

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Ontogeny of the Cytochrome P450 Superfamily in the Ornate Spiny Lobster (Panulirus ornatus)

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(2), P. 1070 - 1070

Published: Jan. 15, 2024

Cytochrome P450s (CYP450s) are a versatile superfamily of enzymes known to undergo rapid evolution. They have important roles across growth and development pathways in crustaceans, although it is difficult characterise orthologs between species due their sequence diversity. Conserved CYP450s crustaceans those associated with ecdysteroidogenesis: synthesising breaking down the active moult hormone, 20-hydroxyecdysone. The complex life cycle ornate spiny lobster, Panulirus ornatus, relies on moulting order grow develop. Many these diverse stages been analysed establish comprehensive transcriptomic database for this species. transcripts putatively encoding were mapped using analysis identified stages. With aid phylogeny, 28 42 putative P. ornatus annotated, including well conserved Halloween genes, which involved ecdysteroidogenesis. Expression patterns determined that only subset can be detected each stage or tissue. Four Shed show overlapping expression metamorphosis adult tissues, suggesting pleotropic functions multiple within ornatus.

Language: Английский

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Effects of molting on the expression of ecdysteroid responsive genes in the crustacean molting gland (Y-organ)

General and Comparative Endocrinology, Journal Year: 2024, Volume and Issue: 355, P. 114548 - 114548

Published: May 16, 2024

Language: Английский

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Integration of ATAC-seq and RNA-seq reveals signal regulation during post-molt and inter-molt stages in muscle of Eriocheir sinensis

Frontiers in Marine Science, Journal Year: 2025, Volume and Issue: 11

Published: Jan. 21, 2025

Introduction The molting processes are crucial for the survival and development of crustaceans. Eriocheir sinensis demonstrates representative discontinuous growth during molting, while muscle is most obvious tissue exhibiting this property. However, signal regulation mechanisms involved in remain unexplored. Methods In work, a comprehensive analysis gene expressions E. between post-molt inter-molt stages were performed by integrating ATAC-seq RNA-seq techniques. Results integration identified 446 up-regulated 21 down-regulated genes two stages. GO enrichment revealed that largely associated with protein phosphorylation phosphorus metabolism, mainly DNA transcription, cell adhesion, G protein-coupled receptor (GPCR) signaling pathway. all enriched pathways, GPCR pathway includes differentially expressed (8 genes), which underlines its importance transduction from stage to stage. Further structure RT-qPCR validation confirmed five related process, four ( GRM7, FMRFaR, mth2, gpr161 ) active one moody functions Discussion These findings highlight key regulatory proteins pathways also offer foundational data studying

Language: Английский

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Effect of blocking transforming growth factor-β/Activin-Myostatin signaling on the expression of ecdysteroid metabolism and responsive genes in the crustacean molting gland (Y-organ)

General and Comparative Endocrinology, Journal Year: 2025, Volume and Issue: 362, P. 114675 - 114675

Published: Feb. 1, 2025

Language: Английский

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Sexual dimorphism alters seasonal chelae muscle mechanisms in spiny-cheek crayfish (Faxonius limosus)

Frontiers in Physiology, Journal Year: 2025, Volume and Issue: 16

Published: April 2, 2025

Sex-specific behaviours of freshwater crayfish are key elements in sustaining species persistence and successful conquering new habitats ecosystems. However, to date, information on molecular mechanisms that underpin the anatomy physiology sexes mating behaviour was scarcely presented. In this study, Faxonius limosus females males were sampled spring autumn assess impact seasons body parameters activity arginine kinase (ak), ferritin (fr), crustacean calcium-binding protein 23 (ccbp-23), troponin c (tnnc), skeletal muscle actin 8 (actinsk8) genes related functioning muscles chelae. Comparison showed significant differences weight size individuals two seasons, underlining large chelae essential for male-male competitive interactions. The gene expression analysis activities five F. influenced by season- sex-specific drivers. Multivariate analyses specifically identified (e.g., tnnc from spring) directly involved metabolisms collected autumn. first time, described direct anatomical features shaped limosus.

Language: Английский

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