Molecular characterization and functional identification of the insulin-like peptides receptor gene in the oriental river prawn Macrobrachium nipponense DOI Creative Commons
Fajun Li,

Xiaocui Cui,

Meina Mao

et al.

Aquaculture Reports, Journal Year: 2024, Volume and Issue: 36, P. 102190 - 102190

Published: June 1, 2024

The insulin-like signaling pathway plays diverse biological roles in regulating metabolism, immune responses, growth, and development invertebrates. peptides (ILP) receptor (ILPR) is the pivotal protein any pathway. In our previous study, we cloned Mn-ILP gene from oriental river prawn Macrobrachium nipponense elucidated its function. However, ILPR has not yet been described M. nipponense. present further examined function of Mn-ILPR full length mRNA was 4790 bp, encoding 1412 amino acids. a transmembrane under subfamily tyrosine kinases. predominantly expressed muscle hepatopancreas. To elucidate Mn-ILPR, investigated expression different growth stages prawns both sexes, sizes at same time, size molt cycle using qRT-PCR. markedly higher: (1) rapid stage than other male individuals; (2) normal gonad female (3) large individuals small (4) intermolt cycle. Furthermore, long-term knockdown by RNAi effectively delayed speed Meanwhile, short-term revealed that ILP-ILPR signal played part process These findings provide greater understanding molecular mechanisms underlying crustaceans.

Language: Английский

Integration of transcriptome and whole-genome re-sequencing analyses reveal growth-related candidate genes in Procambarus clarkii DOI

Zihao Zhou,

Yanhe Li, Yu‐Dong Shen

et al.

Comparative Biochemistry and Physiology Part D Genomics and Proteomics, Journal Year: 2024, Volume and Issue: 49, P. 101198 - 101198

Published: Feb. 1, 2024

Language: Английский

Citations

2
Reproductive Ability Disparity in the Pacific Whiteleg Shrimp (Penaeus vannamei): Insights from Ovarian Cellular and Molecular Levels DOI Creative Commons
Jianchun Zhang, Jie Kong,

Jiawang Cao

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(4), P. 218 - 218

Published: March 27, 2024

The Pacific whiteleg shrimp (Penaeus vannamei) is a highly significant species in aquaculture. In the production of larvae, noticeable variations reproductive capacity among female individuals have been observed. Some females experience slow gonadal development, resulting inability to spawn, while others undergo multiple maturations and contribute majority larval supply. Despite numerous studies that conducted on regulatory mechanisms ovarian development shrimp, factors contributing differences remain unclear. To elucidate underlying mechanisms, this study examined characteristics between high low bulks at different maturity stages, focusing cellular molecular levels. Transmission electron microscopy analysis revealed abundance endoplasmic reticulum, ribosomes, mitochondria, mitochondrial cristae oocytes bulk was significantly higher than early stages maturation (stages I II). As ovaries progressed late-stage III IV), internal structures with capacities gradually diminished. Transcriptome identified differentially expressed genes (DEGs) related mitochondria two groups, suggesting energy processes might play crucial role observed ovary development. expression levels ETS homology factor (EHF) PRDI-BF1 RIZ domain containing 9 (PRDM9), which were compared using qRT-PCR maturation. results showed EHF gene II IV bulk, almost no detected eyestalk tissue bulk. PRDM9 exclusively tissue, four Fluorescence situ hybridization further patterns fertility levels, both showing stronger positive signals stages. These findings not only our understanding involved but also provide valuable insights for cultivation new varieties aimed improving fecundity.

Language: Английский

Citations

2
Phylogenetic and transcriptomic characterization of insulin and growth factor receptor tyrosine kinases in crustaceans DOI Creative Commons

Kaylie A. Flores,

Jorge L. Pérez‐Moreno,

David S. Durica

et al.

Frontiers in Endocrinology, Journal Year: 2024, Volume and Issue: 15

Published: April 4, 2024

Receptor tyrosine kinases (RTKs) mediate the actions of growth factors in metazoans. In decapod crustaceans, RTKs are implicated various physiological processes, such molting and growth, limb regeneration, reproduction sexual differentiation, innate immunity. organized into two main types: insulin receptors (InsRs) factor receptors, which include epidermal receptor (EGFR), fibroblast (FGFR), vascular endothelial (VEGFR), platelet-derived (PDGFR). The identities crustacean RTK genes incomplete. A phylogenetic analysis CrusTome transcriptome database, included all major taxa, showed that sequences segregated clades representing InsR (72 sequences), EGFR (228 FGFR (129 PDGFR/VEGFR (PVR; 235 sequences). These four families were distinguished by domain organization extracellular N-terminal region motif protein kinase catalytic C-terminus or ligand-binding N-terminus. EGFR1 formed a single monophyletic group, while other divided subclades, designated InsR1-3, FGFR1-3, PVR1-2. decapods, isoforms within subclades common. InsRs characterized leucine-rich repeat, furin-like cysteine-rich, fibronectin type 3 domains EGFRs had IV domains. regions FGFR1 one to three immunoglobulin-like domains, whereas FGFR2 cadherin tandem repeat domain. PVRs between five classification nomenclature classes, based on multiple sequence alignments, is proposed.

Language: Английский

Citations

2
Physical Forces in Regeneration of Cells and Tissues DOI
Sindy K. Y. Tang, Wallace F. Marshall

Cold Spring Harbor Perspectives in Biology, Journal Year: 2024, Volume and Issue: unknown, P. a041527 - a041527

Published: May 28, 2024

The ability to regenerate after the loss of a part is hallmark living systems and occurs at both tissue organ scales, but also within individual cells. Regeneration entails many processes that are physical mechanical in nature, including closure wounds, repositioning material from one place another, restoration symmetry following perturbations. However, we currently know far more about genetics molecular signaling pathways involved regeneration, there need investigate role forces process. Here, will provide an overview how may play wound healing which compare contrast regenerative cell scales.

Language: Английский

Citations

2
Molecular characterization and functional identification of the insulin-like peptides receptor gene in the oriental river prawn Macrobrachium nipponense DOI Creative Commons
Fajun Li,

Xiaocui Cui,

Meina Mao

et al.

Aquaculture Reports, Journal Year: 2024, Volume and Issue: 36, P. 102190 - 102190

Published: June 1, 2024

The insulin-like signaling pathway plays diverse biological roles in regulating metabolism, immune responses, growth, and development invertebrates. peptides (ILP) receptor (ILPR) is the pivotal protein any pathway. In our previous study, we cloned Mn-ILP gene from oriental river prawn Macrobrachium nipponense elucidated its function. However, ILPR has not yet been described M. nipponense. present further examined function of Mn-ILPR full length mRNA was 4790 bp, encoding 1412 amino acids. a transmembrane under subfamily tyrosine kinases. predominantly expressed muscle hepatopancreas. To elucidate Mn-ILPR, investigated expression different growth stages prawns both sexes, sizes at same time, size molt cycle using qRT-PCR. markedly higher: (1) rapid stage than other male individuals; (2) normal gonad female (3) large individuals small (4) intermolt cycle. Furthermore, long-term knockdown by RNAi effectively delayed speed Meanwhile, short-term revealed that ILP-ILPR signal played part process These findings provide greater understanding molecular mechanisms underlying crustaceans.

Language: Английский

Citations

2