Transcriptomic Profiling of Subcutaneous Adipose Tissue in Relation to Bariatric Surgery: A Retrospective, Pooled Re-analysis DOI Creative Commons
Youdinghuan Chen

Journal of Obesity & Metabolic Syndrome, Journal Year: 2023, Volume and Issue: 32(1), P. 98 - 102

Published: Feb. 1, 2023

Background: Bariatric surgery is the most effective intervention for weight loss possibly through modulating subcutaneous adipose tissue (SAT) molecular programs.The post-operative and biological impacts, including gene expression, deserve in-depth investigation especially given small sample sizes in literature.Methods: Five existing datasets (n=237 SATs) were re-processed corrected batch-to-batch variation.Unsupervised approaches robust linear mixed effect model used to compare expression post-(n=126) pre-operation (n=111).Results: Post-operative SATs showed distinct global expression.Forty-four 395 genes over-and under-expressed post-operation (all Bonferroni P<0.05).The significantly enriched 21 processes/pathways P<0.05), 17 (76.2%)and two (9.5%) directly involved immunity amino/proteo-glycan metabolism, respectively.Conclusion: might adopt transcriptomic landscapes undergo a reduction immune-related processes metabolism.

Language: Английский

Glycine and aging: Evidence and mechanisms DOI Creative Commons
Adiv A. Johnson, Trinna Cuellar

Ageing Research Reviews, Journal Year: 2023, Volume and Issue: 87, P. 101922 - 101922

Published: March 31, 2023

The restriction of calories, branched-chain amino acids, and methionine have all been shown to extend lifespan in model organisms. Recently, glycine was found boost longevity genetically heterogenous mice. This simple acid similarly extends rats improves health mammalian models age-related disease. While compelling data indicate that is a pro-longevity molecule, divergent mechanisms may underlie its effects on aging. Glycine abundant collagen, building block for glutathione, precursor creatine, an acceptor the enzyme N-methyltransferase (GNMT). A review literature strongly implicates GNMT, which clears from body by taking methyl group S-adenosyl-L-methionine methylating form sarcosine. In flies, Gnmt required reduced insulin/insulin-like growth factor 1 signaling dietary fully lifespan. geroprotector spermidine requires upregulate autophagy genes longevity. Moreover, overexpression sufficient reduce levels. Sarcosine, or methylglycine, declines with age multiple species capable inducing both vitro vivo. Taken together, existing evidence suggests prolongs life mimicking activating autophagy.

Language: Английский

Citations

23
Glycine homeostasis requires reverse SHMT flux DOI Creative Commons
Matthew J. McBride,

Craig Hunter,

Zhaoyue Zhang

et al.

Cell Metabolism, Journal Year: 2024, Volume and Issue: 36(1), P. 103 - 115.e4

Published: Jan. 1, 2024

Language: Английский

Citations

15
Amino Acid Metabolism and Atherosclerotic Cardiovascular Disease DOI Open Access
Sumit Kumar Anand, Theresea-Anne Governale, Xiangyu Zhang

et al.

American Journal Of Pathology, Journal Year: 2024, Volume and Issue: 194(4), P. 510 - 524

Published: Jan. 1, 2024

Language: Английский

Citations

9
Plasma metabolites of a healthy lifestyle in relation to mortality and longevity: Four prospective US cohort studies DOI Creative Commons
Anne‐Julie Tessier, Fenglei Wang, Liming Liang

et al.

Med, Journal Year: 2024, Volume and Issue: 5(3), P. 224 - 238.e5

Published: Feb. 15, 2024

A healthy lifestyle is associated with a lower premature mortality risk and longer life expectancy. However, the metabolic pathways of how they relate to longevity are unclear. We aimed identify replicate metabolomic signature examine it related total cause-specific longevity.

Language: Английский

Citations

8
Exploring the Role of Glycine Metabolism in Coronary Artery Disease: Insights from Human Genetics and Mouse Models DOI Open Access
Subarna Biswas, James R. Hilser,

Nicholas C. Woodward

et al.

Nutrients, Journal Year: 2025, Volume and Issue: 17(1), P. 198 - 198

Published: Jan. 6, 2025

Background: Circulating glycine levels have been associated with reduced risk of coronary artery disease (CAD) in humans but these associations not observed all studies. We evaluated whether the relationship between and atherosclerosis was causal using genetic analyses feeding studies mice. Methods: Serum were for association CAD UK Biobank. Genetic determinants identified through a genome-wide study (GWAS) used to evaluate by Mendelian randomization (MR). A dietary supplementation carried out atherosclerosis-prone apolipoprotein E deficient (ApoE−/−) mice determine effects increased circulating on cardiometabolic traits aortic lesion formation. Results: Among 105,718 Biobank subjects, elevated serum significantly prevalent (Quintile 5 vs. Quintile 1 OR = 0.76, 95% CI 0.67–0.87; p < 0.0001) incident HR 0.70, 0.65–0.77; after adjustment age, sex, ethnicity, anti-hypertensive lipid-lowering medications, blood pressure, kidney function, diabetes. GWAS meta-analysis 230,947 subjects 61 loci levels, which 26 novel. MR provided modest evidence that genetically causally systolic pressure type 2 diabetes, did provide significant an decreased CAD. Glycine had no or atherosclerotic development. Conclusions: While expanding architecture metabolism, vivo clinical this amino acid represents relationship.

Language: Английский

Citations

0
Alterations in glycine metabolism in obesity and chronic metabolic diseases – an update on new advances DOI
Anaïs Alves, Béatrice Morio

Current Opinion in Clinical Nutrition & Metabolic Care, Journal Year: 2022, Volume and Issue: 26(1), P. 50 - 54

Published: Nov. 7, 2022

Purpose of review The metabolic signature associated with obesity is characterized by a decrease in plasma glycine concentration, feature closely insulin resistance and highly predictive the risk developing chronic diseases. This presents recent advances understanding causes decreased availability targeting strategies to replenish pool especially improve resistance. Recent results literature has made progress host gut microbiota mechanisms determining circulating levels. It also explored new clinical pathways restore levels obesity-related Summary findings suggest that metabolism must now be considered close interaction branched-chain amino acid (BCAA) metabolism. Thus, BCAAs seem best glycine. Furthermore, confirmed lifestyle aimed at inducing weight loss are effective replenishing pool. confirms correcting dysbiosis may valuable means achieving this goal. However, it remains unclear whether dietary an strategy for cardiometabolic disorders obesity.

Language: Английский

Citations

17
The impact of obesity-associated glycine deficiency on the elimination of endogenous and exogenous metabolites via the glycine conjugation pathway DOI Creative Commons
Hong Chang Tan, Jean W. Hsu, E Shyong Tai

et al.

Frontiers in Endocrinology, Journal Year: 2024, Volume and Issue: 15

Published: April 3, 2024

Background Glycine is an integral component of the human detoxification system as it reacts with potentially toxic exogenous and endogenously produced compounds metabolites via glycine conjugation pathway for urinary excretion. Because individuals obesity have reduced availability, this may be compromised. However, should restored after bariatric surgery because increased production. Objective To examine impact obesity-associated deficiency on pathway. We hypothesize that synthesis rates acylglycines from endogenous sources are significantly in but increase surgery. Methods recruited 21 participants class III healthy weight controls. At baseline, [1,2- 13 C 2 ] was infused to study by quantifying several acylglycines. The same measurements were repeated six months Data presented mean ± standard deviation, p -value&lt; 0.05 considered statistically significant. Results Baseline data 20 first compared Participants heavier than controls (mean BMI 40.5 7.1 vs. 20.8 2.1 kg/m ). They had lower plasma concentration (168 30 209 50 μmol/L) slower absolute acetylglycine, isobutyrylglycine, tigylglycine, isovalerylglycine, hexanoylglycine. Pre- post-surgery available 16 obesity. Post-surgery decreased 40.9 7.3 31.6 6.0 . Plasma 164 26 212 38 associated higher excretion Benzoic acid (a xenobiotic dicarboxylic acid) excreted benzoylglycine; its rate Conclusion Obesity-associated impairs body’s ability eliminate metabolites/compounds This impairment ameliorated when supply These findings imply dietary supplementation could treat metabolic complications due accumulation intramitochondrial metabolites. Clinical trial registration https://clinicaltrials.gov/study/NCT04660513 , identifier NCT04660513.

Language: Английский

Citations

3
Reduced plasma glycine concentration in healthy and chronically diseased older adults: a marker of visceral adiposity? DOI

Lars NJ Deutz,

Raven Wierzchowska-McNew, Nicolaas E.P. Deutz

et al.

American Journal of Clinical Nutrition, Journal Year: 2024, Volume and Issue: 119(6), P. 1455 - 1464

Published: April 12, 2024

Language: Английский

Citations

2
Impact of Bariatric Surgery on the Stability of the Genetic Material, Oxidation, and Repair of DNA and Telomere Lengths DOI Creative Commons
Franziska Ferk, Miroslav Mišík, Benjamin Ernst

et al.

Antioxidants, Journal Year: 2023, Volume and Issue: 12(3), P. 760 - 760

Published: March 21, 2023

Obesity causes genetic instability, which plays a key-role in the etiology of cancer and aging. We investigated impact bariatric surgery (BS) on DNA repair, oxidative damage, telomere lengths, alterations antioxidant enzymes and, selected proteins reflect inflammation. The study was realized with BS patients (n = 35). base oxidation, BER, NER were measured before 1 month 6 months after single-cell gel electrophoresis technique. SOD GPx quantified spectrophotometrically, malondealdehyde (MDA) by HPLC. Telomere lengths determined qPCR, plasma proteome profiling performed high-resolution mass spectrophotometry. Six operations, reduction body weight 27.5% observed. damage decreased this period, effect paralleled reduced formation oxidized bases, decline MDA levels BER NER, an increase lengths. activities not altered. Clear downregulation certain (CRP, SAA1) inflammation risks Our findings show that possibly as consequence lipid peroxidation, indicate has beneficial long-term health effects.

Language: Английский

Citations

6
Growth Hormone Alters Circulating Levels of Glycine and Hydroxyproline in Mice DOI Creative Commons
J. A. Young, Silvana Duran‐Ortiz, Stephen Bell

et al.

Metabolites, Journal Year: 2023, Volume and Issue: 13(2), P. 191 - 191

Published: Jan. 28, 2023

Growth hormone (GH) has established effects on protein metabolism, such as increasing synthesis and decreasing amino acid degradation, but its circulating levels are less studied. To investigate this relationship, metabolomic analyses were used to measure concentrations in plasma feces of mice with alterations the GH axis, namely bovine transgenic (bGH; increased action) receptor knockout (GHRKO; resistant) mice. determine acute treatment, GH-injected (GHKO) serum glycine. Furthermore, liver gene expression glycine metabolism genes was assessed bGH, GHRKO, GHKO bGH had significantly decreased hydroxyproline both sexes, while GHRKO sexes males. Glycine (Shmt1 females Shmt2 males) (Shmt2 males). Acute treatment caused Shmt1 In conclusion, alters opposing directions, changes at least partially driven by synthesis.

Language: Английский

Citations

5