Growth Hormone Action as a Target in Cancer: Significance, Mechanisms and Possible Therapies DOI Creative Commons
Reetobrata Basu, César Luiz Boguszewski, John J. Kopchick

et al.

Endocrine Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 3, 2024

Growth hormone (GH) is a pituitary derived endocrine required for normal post-natal growth and development. Hypo or hypersecretion of GH results in two pathologic conditions, namely deficiency (GHD) acromegaly. Additionally, also produced non-pituitary tumoral tissues where it acts rather as cellular factor with an autocrine/paracrine mode action. An increasingly persuasive large body evidence over the last 70 years concur that action implicit escalating several cancer-associated events, locally systemically. This pleiotropy GH's effects puzzling, but association cancer-risk automatically raises concern patients acromegaly individuals treated GH. By careful assessment available knowledge on fundamental concepts cancer, suggestions from epidemiological clinical studies, specific reports, this review we aimed to help clarify distinction vs. promoting cancer reconcile discrepancies between experimental data. Along discourse, critically weigh targetability - firstly by detailing molecular mechanisms which posit critical node tumor circuitry, secondly, enumerating currently therapeutic options targeting On basis our discussion, infer targeted intervention appropriate patient population can benefit sizeable subset current prognoses.

Language: Английский

Growth Hormone Receptor Antagonist Markedly Improves Gemcitabine Response in a Mouse Xenograft Model of Human Pancreatic Cancer DOI Open Access
Reetobrata Basu, Prateek Kulkarni,

Deborah Swegan

et al.

Published: May 13, 2024

Chemotherapy treatment against pancreatic ductal adenocarcinoma (PDAC) is thwarted by tumoral activation of multiple therapy-resistance pathways. The growth hormone (GH) – GH receptor (GHR) pair a covert driver multimodal therapy resistance in cancer, and overexpressed PDAC tumors, yet the therapeutic potential targeting same has not been explored. Here, we report that GHR expression negative prognostic factor patients with PDAC. Combinations gemcitabine different antagonists (GHRAs) markedly improve outcomes nude mice xenografts. Employing cultured cells, mouse xenografts, analyses human transcriptome, identified attenuation multidrug transporter epithelial-to-mesenchymal transition programs tumors underlie observed augmentation chemotherapy efficacy GHRAs. Moreover, patients, strongly correlates gene signature tumor-promotion immune-evasion, which corroborate syngeneic wild-type vs. transgenic mice. Overall, found action promoted therapy-refractory vivo, can be effectively attenuated antagonism. Our results collectively present proof concept towards considering antagonist to chemotherapeutic outcome highly chemoresistant

Language: Английский

Citations

3

Growth Hormone Receptor Antagonist Markedly Improves Gemcitabine Response in a Mouse Xenograft Model of Human Pancreatic Cancer DOI Open Access
Reetobrata Basu, Prateek Kulkarni,

Deborah Swegan

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(13), P. 7438 - 7438

Published: July 6, 2024

Chemotherapy treatment against pancreatic ductal adenocarcinoma (PDAC) is thwarted by tumoral activation of multiple therapy resistance pathways. The growth hormone (GH)-GH receptor (GHR) pair a covert driver multimodal in cancer and overexpressed PDAC tumors, yet the therapeutic potential targeting same has not been explored. Here, we report that GHR expression negative prognostic factor patients with PDAC. Combinations gemcitabine different antagonists (GHRAs) markedly improve outcomes nude mice xenografts. Employing cultured cells, mouse xenografts, analyses human transcriptome, identified attenuation multidrug transporter epithelial-to-mesenchymal transition programs tumors underlie observed augmentation chemotherapy efficacy GHRAs. Moreover, patients, strongly correlates gene signature tumor promotion immune evasion, which corroborate syngeneic wild-type vs. GH transgenic mice. Overall, found action promoted therapy-refractory vivo, can be effectively attenuated antagonism. Our results collectively present proof concept toward considering to chemotherapeutic highly chemoresistant

Language: Английский

Citations

2

Common and Uncommon Mouse Models of Growth Hormone Deficiency DOI Creative Commons
Edward O. List, Reetobrata Basu, Darlene E. Berryman

et al.

Endocrine Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: June 10, 2024

Abstract Mouse models of growth hormone deficiency (GHD) have provided important tools for uncovering the various actions GH. Nearly 100 years research using these mouse lines has greatly enhanced our knowledge GH/IGF-1 axis. Some shared phenotypes 5 “common” GHD include reduced body size, delayed sexual maturation, decreased fertility, muscle mass, increased adiposity, and insulin sensitivity. Since common outlive their normal-sized littermates—and protection from age-associated disease—they become fixtures in aging field. On other hand, 12 “uncommon” described herein tremendously divergent health outcomes ranging beneficial (similar to those models) extremely detrimental features (such as improper development central nervous system, numerous sensory organ defects, embryonic lethality). Moreover, advancements next-generation sequencing technologies led identification an expanding array genes that are recognized causative agents rare syndromes with concomitant GHD. Accordingly, this review provides researchers a comprehensive up-to-date collection uncommon been used study aspects physiology metabolism associated multiple forms For each line presented, closest comparable human discussed providing parallels clinic.

Language: Английский

Citations

1

Characterizing Hormone Secretion Patterns in PitNETs with Metabolomics: Implications for Understanding Tumor Biology DOI Creative Commons
Fatmanur Köktaşoğlu, Metin Demirel, Halime Dulun Ağaç

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 17, 2024

Abstract Background Pituitary neuroendocrine tumors (PitNETs) are heterogeneous neoplasms originating from the pituitary gland. Metabolomics, a comprehensive analysis of small molecules, has emerged as valuable tool for studying tumors. In presen investigation, metabolomic methodology was employed to facilitate more understanding tumor pathogenesis. Methods Nuclear Magnetic Resonance (NMR) Spectroscopy utilized investigate metabolic profiles hypophyseal tissue samples obtained 22 patients with PitNETs, who underwent excisional surgery and exhibited varying hormone secretion statuses. Results Using NMR analysis, we identified 10 metabolites significant changes, including O-Phosphoethanolamine (PEA), myo-Inositol (I), choline, several amino acids in samples. non-functioning (NF) group, elevated levels PEA, myo-I, Glycine, Choline were observed, whereas Glutamate, Phenylalanine, Valine, Isoleucine, Tyrosine, Methionine decreased same group. Phospholipid metabolism, inositol phosphate acid metabolism proposed potential mechanisms underlying secretory characteristics tissue. Conclusions Functioning nonfunctioning PitNETs display distinct characteristics. Elevated PEA observed group might have inhibited synthesis by suppressing mitochondrial activity, which could potentially contribute development Further research is warranted validate these findings explore their clinical applications, such biomarker discovery therapeutic targeting

Language: Английский

Citations

0

Growth Hormone Action as a Target in Cancer: Significance, Mechanisms and Possible Therapies DOI Creative Commons
Reetobrata Basu, César Luiz Boguszewski, John J. Kopchick

et al.

Endocrine Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 3, 2024

Growth hormone (GH) is a pituitary derived endocrine required for normal post-natal growth and development. Hypo or hypersecretion of GH results in two pathologic conditions, namely deficiency (GHD) acromegaly. Additionally, also produced non-pituitary tumoral tissues where it acts rather as cellular factor with an autocrine/paracrine mode action. An increasingly persuasive large body evidence over the last 70 years concur that action implicit escalating several cancer-associated events, locally systemically. This pleiotropy GH's effects puzzling, but association cancer-risk automatically raises concern patients acromegaly individuals treated GH. By careful assessment available knowledge on fundamental concepts cancer, suggestions from epidemiological clinical studies, specific reports, this review we aimed to help clarify distinction vs. promoting cancer reconcile discrepancies between experimental data. Along discourse, critically weigh targetability - firstly by detailing molecular mechanisms which posit critical node tumor circuitry, secondly, enumerating currently therapeutic options targeting On basis our discussion, infer targeted intervention appropriate patient population can benefit sizeable subset current prognoses.

Language: Английский

Citations

0