Same same, but different: exploring the enigmatic role of the pituitary adenylate cyclase- activating polypeptide (PACAP) in invertebrate physiology DOI Creative Commons
Zsolt Pirger, Péter Urbán, Bence Gálik

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Feb. 28, 2023

Abstract There is a long-standing debate about the presence and functionality of pituitary adenylate cyclase-activating polypeptide (PACAP) in non-bilaterians, protostomes, invertebrate deuterostomes. Evidence has been accumulating that homologous sequences to genes encoding PACAP peptides their receptors vertebrates are missing genomes. This at odds, however, with partial sequence-, immunohistochemical-, physiological evidence literature. In this study, we first sequenced neural transcriptome widely used model species, great pond snail ( Lymnaea stagnalis ), then screened it for elements vertebrate system. Further, performed vitro vivo studies on heart L. explore enigmatic role physiology. Our thorough screening failed identify putative transcripts (or genes) prepropeptides, active peptides, receptors. Despite lack relevant sequences, our immunohistochemical investigations an anti-human PAC 1 receptor antibody yielded positive signal neuronal heart. Although gel electrophoretic separation, followed by immunostaining, proteins extracted from central nervous system found band PACAP-38, mass spectrometric analysis did not find any corresponding peptide sequences. Similarly effects reported vertebrates, 10 µM synthetic PACAP-38 significantly increased cAMP synthesis homogenate had ionotropic effect isolated preparations. Moreover, modulated serotonin acetylcholine. findings support idea absent mollusks emerged after protostome-deuterostome divergence. The no matter how similar they those should be considered non-specific. Further aimed investigating cellular molecular underpinnings including identification which may bind non-specifically.

Language: Английский

Exploring BPA alternatives – Environmental levels and toxicity review DOI Creative Commons
Ondřej Adamovský, Ksenia J. Groh, Anna Białk‐Bielińska

et al.

Environment International, Journal Year: 2024, Volume and Issue: 189, P. 108728 - 108728

Published: May 23, 2024

Bisphenol A alternatives are manufactured as potentially less harmful substitutes of bisphenol (BPA) that offer similar functionality. These already in the market, entering environment and thus raising ecological concerns. However, it can be expected levels BPA will dominate future, they limited information on their environmental safety. The EU PARC project highlights priority chemicals consolidates alternatives, with a focus relevance identification research gaps. review highlighted aspects future perspectives. In brief, an extension monitoring is crucial, extending to cover track facilitate timely implementation mitigation measures. biological activity has been studied for but non-systematic way prioritized number chemicals. For several data provided substantial evidence regarding potential harm environment. We stress importance conducting more comprehensive assessments go beyond traditional reproductive studies overlooked relevant endpoints. Future should also consider mixture effects, realistic concentrations, long-term consequences biota ecosystems.

Language: Английский

Citations

17
Toxicity and bioconcentration of bisphenol A alternatives in the freshwater pulmonate snail Planorbella pilsbryi DOI Creative Commons
Ève A.M. Gilroy, Karyn Robichaud,

Maria Villella

et al.

Environmental Science and Pollution Research, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

Language: Английский

Citations

1
Same same, but different: exploring the enigmatic role of the pituitary adenylate cyclase-activating polypeptide (PACAP) in invertebrate physiology DOI Creative Commons
Zsolt Pirger, Péter Urbán, Bence Gálik

et al.

Journal of Comparative Physiology A, Journal Year: 2024, Volume and Issue: 210(6), P. 909 - 925

Published: June 28, 2024

Evidence has been accumulating that elements of the vertebrate pituitary adenylate cyclase-activating polypeptide (PACAP) system are missing in non-chordate genomes, which is at odds with partial sequence-, immunohistochemical-, and physiological data literature. Multilevel experiments were performed on great pond snail (Lymnaea stagnalis) to explore role PACAP invertebrates. Screening neuronal transcriptome genome did not reveal homologs system. Despite this, immunohistochemical investigations an anti-human PAC

Language: Английский

Citations

2
The genome of the simultaneously hermaphroditic snail Lymnaea stagnalis reveals an evolutionary expansion of FMRFamide-like receptors DOI Creative Commons
Joris M. Koene, Daniel J. Jackson, Yumi Nakadera

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 12, 2024

Abstract The great pond snail Lymnaea stagnalis has served as a model organism for over century in diverse disciplines such neurophysiology, evolution, ecotoxicology and developmental biology. To support both established uses newly emerging research interests we have performed whole genome sequencing (~ 176 x depth), assembly annotation of single individual derived from an inbred line. These efforts resulted final 943 Mb (L50 = 257; N50 957,215) with total 22,499 predicted gene models. As first step towards understanding the hermaphroditic reproductive biology L. stagnalis, identified molecular receptors, specifically nuclear receptors (including discovered 2xDNA binding domain-NRs), G protein-coupled receptor tyrosine kinases, that may be involved cellular specification maintenance simultaneously active male female systems. A phylogenetic analysis one particular family GPCRs (Rhodopsin neuropeptide FMRFamide-receptor-like genes) shows remarkable expansion coincides occurrence simultaneous hermaphroditism Euthyneura gastropods. some NRs also showed qualitative differences expression (albumen gland) (prostate organs, it is possible separate regulation processes part been enabled by increased abundance transition separate-sexed state to condition. findings will pair their activating ligands, more generally stimulate deeper insight into mechanisms underlie modes action compounds neuroendocrine reproduction, induced toxicity, development molluscs general.

Language: Английский

Citations

1
Spermatogenesis and Spermiogenesis in the Mollusca DOI
Alan N. Hodgson,

Kevin J. Eckelbarger

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

Citations

0
Characterization of corazonin signaling in a molluscan model species, Lymnaea stagnalis DOI Creative Commons
I Fodor, Tomohiro Osugi, Shin Matsubara

et al.

General and Comparative Endocrinology, Journal Year: 2024, Volume and Issue: 357, P. 114594 - 114594

Published: July 22, 2024

In recent years, new concepts have emerged regarding the nomenclature, functions, and relationships of different peptide families gonadotropin-releasing hormone (GnRH) superfamily. One main driving forces for this originated from emerging evidence that neuropeptides previously called molluscan GnRH are multifunctional should be classified as corazonin (CRZ). However, research articles still appear use incorrect nomenclature attribute same function to CRZs vertebrate GnRHs. The aim present study was further support interpretation origin Towards goal, we report characterization CRZ signaling system in model species, great pond snail (Lymnaea stagnalis). We detected a CRZ-receptor-like sequence (Lym-CRZR) by homology-searching Lymnaea transcriptomes deduced amino acid showed high similarity receptors receptors. Molecular phylogenetic tree analysis demonstrated Lym-CRZR is included cluster CRZRs. transiently transfected into HEK293 cells found localized at plasma membrane, confirming it functions membrane receptor, like other G protein-coupled assays revealed identified Lym-CRZ neuropeptide stimulated intracellular Ca2+ mobilization dose-dependent manner, but not cyclic AMP production, with Lym-CRZR. Finally, wide tissue distribution These results suggest provide insights evolution also supports notion termed "GnRH" "CRZ".

Language: Английский

Citations

0
Lack of membrane sex steroid receptors for mediating rapid endocrine responses in molluscan nervous systems DOI Creative Commons
I Fodor, Shin Matsubara, Tomohiro Osugi

et al.

Frontiers in Endocrinology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 12, 2024

Despite the lack of endogenous synthesis and relevant nuclear receptors, several papers have been published over decades claiming that physiology mollusks is affected by natural synthetic sex steroids. With scant evidence for existence functional steroid receptors in mollusks, some scientists speculated effects steroids might be mediated via membrane (i.e. non-genomic/non-classical actions) - a mechanism has well-characterized vertebrates. However, no study yet investigated ligand-binding ability such receptor candidates mollusks. The aim present was to further trace evolution endocrine system investigating presence mollusk, great pond snail ( Lymnaea stagnalis ). We detected sequences homologous known vertebrate transcriptome genome data: G protein-coupled estrogen receptor-1 (GPER1); progestin (mPRs); family C group 6 member A (GPRC6A); Zrt- Irt-like protein 9 (ZIP9). Sequence analyses, including conserved domain analysis, phylogenetics, transmembrane prediction, indicated mPR ZIP9 appeared homologs, while GPER1 GPRC6A seemed non-orthologous receptors. All transiently transfected into HEK293MSR cells were found localized at plasma membrane, confirming they function as signaling assays revealed none interacted with main ligands. Our findings strongly suggest which would ones are not . Although experiments required on other molluscan model species well, we propose both classical non-classical responses specific chordates, systems fundamentally different.

Language: Английский

Citations

0
Aquatic invertebrate endocrine disruption DOI

Enmin Zou

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Citations

0
The genome of the simultaneously hermaphroditic snail Lymnaea stagnalis reveals an evolutionary expansion of FMRFamide-like receptors DOI Creative Commons
Joris M. Koene, Daniel J. Jackson, Yumi Nakadera

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 25, 2024

The great pond snail Lymnaea stagnalis has served as a model organism for over century in diverse disciplines such neurophysiology, evolution, ecotoxicology and developmental biology. To support both established uses newly emerging research interests we have performed whole genome sequencing (avg.176 × depth), assembly annotation of single individual derived from an inbred line. These efforts resulted final 943 Mb (L50 = 257; N50 957,215) with total 22,499 predicted gene models. mitogenome was found to be 13,834 bp long similarly organized other lymnaeid species, minor differences location tRNA genes. As first step towards understanding the hermaphroditic reproductive biology L. stagnalis, identified molecular receptors, specifically nuclear receptors (including discovered 2xDNA binding domain-NRs), G protein-coupled receptor tyrosine kinases, that may involved cellular specification maintenance simultaneously active male female systems. A phylogenetic analysis one particular family GPCRs (Rhodopsin neuropeptide FMRFamide-receptor-like genes) shows remarkable expansion coincides occurrence simultaneous hermaphroditism Euthyneura gastropods. some NRs also showed qualitative expression (albumen gland) (prostate organs, it is possible separate regulation processes part been enabled by increased abundance transition separate-sexed state condition. findings will pair their activating ligands, more generally stimulate deeper insight into mechanisms underlie modes action compounds neuroendocrine reproduction, induced toxicity, development molluscs general.

Language: Английский

Citations

0
Same same, but different: exploring the enigmatic role of the pituitary adenylate cyclase- activating polypeptide (PACAP) in invertebrate physiology DOI Creative Commons
Zsolt Pirger, Péter Urbán, Bence Gálik

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Feb. 28, 2023

Abstract There is a long-standing debate about the presence and functionality of pituitary adenylate cyclase-activating polypeptide (PACAP) in non-bilaterians, protostomes, invertebrate deuterostomes. Evidence has been accumulating that homologous sequences to genes encoding PACAP peptides their receptors vertebrates are missing genomes. This at odds, however, with partial sequence-, immunohistochemical-, physiological evidence literature. In this study, we first sequenced neural transcriptome widely used model species, great pond snail ( Lymnaea stagnalis ), then screened it for elements vertebrate system. Further, performed vitro vivo studies on heart L. explore enigmatic role physiology. Our thorough screening failed identify putative transcripts (or genes) prepropeptides, active peptides, receptors. Despite lack relevant sequences, our immunohistochemical investigations an anti-human PAC 1 receptor antibody yielded positive signal neuronal heart. Although gel electrophoretic separation, followed by immunostaining, proteins extracted from central nervous system found band PACAP-38, mass spectrometric analysis did not find any corresponding peptide sequences. Similarly effects reported vertebrates, 10 µM synthetic PACAP-38 significantly increased cAMP synthesis homogenate had ionotropic effect isolated preparations. Moreover, modulated serotonin acetylcholine. findings support idea absent mollusks emerged after protostome-deuterostome divergence. The no matter how similar they those should be considered non-specific. Further aimed investigating cellular molecular underpinnings including identification which may bind non-specifically.

Language: Английский

Citations

0