Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: June 19, 2024
Abstract
Methylene
blue
(MB)
is
an
antifungal
agent
widely
used
during
critical
stages
of
zebrafish
development.
Most
guidelines
recommend
0.00005%
or
0.0001%
MB
for
embryo/larval
rearing.
The
Organisation
Economic
Co-operation
and
Development
embryo
toxicity
test
guideline
omits
recommendations,
leading
to
inconsistent
use
in
research.
Because
affects
oxidative
energy
metabolism
vitro
vivo,
we
investigated
possible
metabolic
effects
recommended
concentrations
developing
(1-5
days
post-fertilization
(dpf)).
increased
O2
consumption
rate
at
1
dpf,
followed
by
overall
reduction
post-hatch
eleutheroembryos
(4-5
dpf).
Concomitantly,
mitochondrial
transcripts
decreased
4
dpf
zebrafish.
Our
findings
show
that
MB,
husbandry
concentrations,
can
thus
confound
experiments.
Since
the
model
gaining
traction
as
a
high-throughput
New
Approach
Methodology
(NAM)
assessment,
researchers
should
reconsider
use.
Hormones and Behavior,
Journal Year:
2024,
Volume and Issue:
161, P. 105521 - 105521
Published: March 6, 2024
The
neuropeptides
arginine
vasopressin
(AVP)
and
oxytocin
(OXT)
are
key
regulators
of
social
behaviour
across
vertebrates.
However,
much
our
understanding
how
these
neuropeptide
systems
interact
with
is
centred
around
laboratory
studies
which
fail
to
capture
the
physiological
challenges
living
in
wild.
To
evaluate
relationships
between
wild,
we
studied
groups
cichlid
fish
Neolamprologus
pulcher
Lake
Tanganyika,
Africa.
We
first
used
SCUBA
observe
focal
group
members
then
measured
transcript
abundance
components
AVP
OXT
different
brain
regions.
While
often
associated
male-typical
behaviours,
found
that
dominant
females
had
higher
expression
avp
its
receptor
(avpr1a2)
preoptic
area
compared
either
males
or
subordinates
sex.
Dominant
also
generally
highest
levels
leucyl-cystinyl
aminopeptidase
(lnpep)-which
inactivates
OXT-throughout
brain,
potentially
indicating
greater
overall
activity
(i.e.,
production,
release,
turnover)
system
females.
Expression
receptors
did
not
differ
ranks.
visited
brood
chamber
more
lower
a
(oxtra)
suggesting
negative
relationship
signalling
parental
care
this
species.
Overall,
results
advance
complex
behaviours
neuroendocrine
under
natural
settings.
Communications Biology,
Journal Year:
2025,
Volume and Issue:
8(1)
Published: Jan. 25, 2025
Methylene
blue
(MB)
is
an
antifungal
agent
widely
used
during
critical
stages
of
zebrafish
development.
Most
guidelines
recommend
0.00005%
or
0.0001%
MB
for
embryo/larval
rearing.
The
Organisation
Economic
Co-operation
and
Development
embryo
toxicity
test
guideline
omits
recommendations,
leading
to
inconsistent
use
in
research.
Because
affects
oxidative
energy
metabolism
vitro
vivo,
we
investigate
possible
metabolic
effects
recommended
concentrations
developing
(1–5
days
post-fertilization
(dpf)).
increases
O2
consumption
rate
at
1
dpf,
followed
by
overall
reduction
post-hatch
eleutheroembryos
(4–5
dpf).
Concomitantly,
mitochondrial
transcripts
decrease
4
dpf
zebrafish.
Our
findings
show
that
MB,
husbandry
concentrations,
can
thus
confound
experiments.
Since
the
model
gaining
traction
as
a
high-throughput
New
Approach
Methodology
(NAM)
assessment,
researchers
should
reconsider
use.
Blue
additive
medium.
At
it
exerts
acute
effects.
This
represents
previously
unappreciated
Frontiers in Endocrinology,
Journal Year:
2023,
Volume and Issue:
14
Published: Dec. 8, 2023
In
zebrafish,
estrogens
produced
in
the
ovaries
via
Cyp19a1a
activity
are
required
for
both
sexual
differentiation
of
ovary
during
early
development
as
well
maintenance
ovarian
state
adulthood.
The
importance
Cyp19a1b
that
is
highly
expressed
brain
female
reproduction
still
under
study.
We
previously
reported
cyp19a1b
-/-
mutant
zebrafish
have
significantly
lower
estradiol
levels
and
impaired
spawning
behavior
characterized
by
an
increased
latency
to
oviposition
dyadic
encounters.
current
study,
we
provide
evidence
delayed
mutants
linked
arginine
vasopressin
(Avp)
signaling.
Droplet
digital
PCR
experiments
revealed
estrogen
receptors,
avp
,
oxytocin
(
oxt
)
hypothalamus
females
compared
wildtype
fish.
then
used
acute
intraperitoneal
injections
Avp
Oxt,
along
with
mixtures
their
respective
receptor
antagonists,
determine
can
uniquely
rescue
mutants.
Using
immunohistochemistry,
demonstrated
Cyp19a1b-expressing
radial
glial
cell
(RGC)
fibers
surround
contact
Avp-immunopositive
neurons
preoptic
areas
brain.
This
could
neuroanatomical
proximity
RGC-derived
diffuse
activate
receptors
regulate
expression
levels.
Together
these
findings
identify
a
positive
link
between
behavior.
They
also
suggest
behavioral
phenotype
likely
consequence
processing
Avp-dependent
social
cues
important
mate
identification
assessment.
Genes,
Journal Year:
2024,
Volume and Issue:
15(6), P. 726 - 726
Published: June 1, 2024
Aquaculture
supplies
the
world
food
market
with
a
significant
amount
of
valuable
protein.
Highly
productive
aquaculture
fishes
can
be
derived
by
utilizing
genome-editing
methods,
and
main
problem
is
to
choose
target
gene
obtain
desirable
phenotype.
This
paper
presents
review
studies
genome
editing
for
genes
controlling
body
development,
growth,
pigmentation
sex
determination
in
five
key
Salmonidae
Cyprinidae
species,
such
as
rainbow
trout
(Onchorhynchus
mykiss),
Atlantic
salmon
(Salmo
salar),
common
carp
(Cyprinus
carpio),
goldfish
(Carassius
auratus),
Gibel
gibelio)
model
fish
zebrafish
(Danio
rerio).
Among
studied,
most
applicable
are
mstnba,
pomc,
acvr2,
knockout
which
leads
enhanced
muscle
growth;
runx2b,
mutants
do
not
form
bones
myoseptae;
lepr,
whose
lack
function
makes
fast-growing;
fads2,
Δ6abc/5Mt,
Δ6bcMt,
affecting
composition
fatty
acids
meat;
dnd
mettl3,
wnt4a,
sterile;
disease-susceptibility
prmt7,
gab3,
gcJAM-A,
cxcr3.2.
Schemes
obtaining
populations
consisting
only
large
females
promising
use
aquaculture.
The
immobilized
uncolored
line
interest
laboratory
use.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(12), P. 6564 - 6564
Published: June 14, 2024
The
neuropeptide
vasopressin
is
known
for
its
regulation
of
osmotic
balance
in
mammals.
Arginine
vasotocin
(AVT)
a
non-mammalian
homolog
this
that
present
fish.
Limited
information
suggested
and
homologs
may
also
influence
reproductive
function.
In
the
study,
we
investigated
direct
effect
AVT
on
spermatogenesis,
using
zebrafish
as
model
organism.
Results
demonstrate
receptors
(avpr1aa,
avpr2aa,
avpr1ab,
avpr2ab,
avpr2l)
are
expressed
brain
testes.
action
spermatogenesis
was
an
ex
vivo
culture
mature
testes
7
days.
Using
histological,
morphometric,
biochemical
approaches,
observed
actions
testicular
treatment
directly
increased
number
spermatozoa
androgen-dependent
manner,
while
reducing
mitotic
cells
proliferation
activity
type
B
spermatogonia.
stimulatory
spermiogenesis
blocked
by
flutamide,
androgen
receptor
antagonist.
results
support
novel
hypothesis
stimulates
short-term
spermiogenesis.
However,
prolonged
presence
lead
to
diminished
spermatogonia
B,
resulting
turnover
spermatogonia,
spermatids,
spermatozoa.
overall
findings
offer
insight
into
physiological
significance
vertebrates
contributing
factor
multifactorial
male
reproduction.
