DLK1 Is Associated with Stemness Phenotype in Medullary Thyroid Carcinoma Cell Lines DOI Open Access

Danilo Dias da Silva,

Rodrigo Pinheiro Araldi, Mariana Rocha Belizario

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 11924 - 11924

Published: Nov. 6, 2024

Medullary thyroid carcinoma (MTC) is a rare and aggressive tumor, often requiring systemic treatment in advanced or metastatic stages, where drug resistance presents significant challenge. Given the role of cancer stem cells (CSCs) recurrence resistance, we aimed to identify CSC subpopulations within two MTC cell lines harboring pathogenic variants most common MEN2-associated codons. We analyzed 15 stemness-associated markers, along with well-established markers (CD133, CD44, ALDH1), novel candidate (DLK1), multidrug proteins (MRP1 MRP3). The ability efflux fluorescent dye Hoechst 3342 form spheroids, representing behavior, was also assessed. MZ-CRC-1 (p.M918T) displayed higher expressions canonical DLK1, MRP than TT (p.C634W). formed more spheroids showed less accumulation (p < 0.0001). Finally, observed that DLK1+ (those expressing DLK1) both exhibited significantly levels stemness compared DLK1− lacking DLK1 expression). These findings underscore DLK1’s enhancing phenotype, providing valuable insights into progression suggesting potential therapeutic implications.

Language: Английский

The Potential of Peptide-Based Inhibitors in Disrupting Protein–Protein Interactions for Targeted Cancer Therapy DOI Open Access

Ana Maria Sardinha Afonso,

Carlos Cavaleiro, Miguel A. R. B. Castanho

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3117 - 3117

Published: March 28, 2025

Protein-protein interactions (PPIs) form an intricate cellular network known as the interactome, which is essential for various processes, such gene regulation, signal transduction, and metabolic pathways. The dysregulation of this has been closely linked to disease states. In cancer, these aberrant PPIs, termed oncogenic PPIs (OncoPPIs), are involved in tumour formation proliferation. Therefore, inhibition OncoPPIs becomes a strategy targeted cancer therapy. Small molecule inhibitors have dominant PPI owing their small size ability cross cell membranes. However, peptide-based emerged compelling alternatives, offering distinct advantages over inhibitors. Peptides, with larger flexible backbones, can effectively engage broad interfaces PPIs. Their high specificity, lower toxicity, ease modification make them promising candidates Over past decade, significant advancements made developing This review discusses critical aspects targeting emphasizes significance therapy, explores using therapeutic agents. It also highlights recent progress peptide design aimed at overcoming limitations therapeutics, comprehensive overview current landscape potential treatment.

Language: Английский

Citations

1
Circulating Biomarkers of Thyroid Cancer: An Appraisal DOI Open Access
Marta Codrich,

Alessia Biasotto,

Federica DˈAurizio

et al.

Journal of Clinical Medicine, Journal Year: 2025, Volume and Issue: 14(5), P. 1582 - 1582

Published: Feb. 26, 2025

Thyroid cancer is the most prevalent endocrine cancer. The prognosis depends on type and stage at diagnosis. treatments involve surgery, possibly followed by additional therapeutic options such as hormone therapy, radiation targeted therapy chemotherapy. Besides well-known thyroid tumor biomarkers, new circulating biomarkers are now emerging. Advances in genomic, transcriptomic proteomic technologies have allowed development of novel biomarkers. This review explores current literature data to critically analyze benefits limitations routinely measured for diagnosis monitoring also sheds light focusing challenges their use clinical management cancer, underlining need identification a generation

Language: Английский

Citations

0
Identification of thyroid cancer biomarkers using WGCNA and machine learning DOI Creative Commons

Gaofeng Hu,

Wenyuan Niu,

Jiaming Ge

et al.

European journal of medical research, Journal Year: 2025, Volume and Issue: 30(1)

Published: April 5, 2025

Language: Английский

Citations

0
Machine Learning for Thyroid Cancer Detection, Presence of Metastasis, and Recurrence Predictions—A Scoping Review DOI Open Access
Irina-Oana Lixandru-Petre, Alexandru Dima,

Mädälina Muşat

et al.

Cancers, Journal Year: 2025, Volume and Issue: 17(8), P. 1308 - 1308

Published: April 12, 2025

Thyroid Cancer (TC) is one of the most prevalent endocrine malignancies, with early detection being critical for patient management. The motivation integrating Machine Learning (ML) in thyroid cancer research stems from limitations conventional diagnostic and monitoring approaches, as ML offers transformative potential reducing human errors improving prediction outcomes accuracy, risk stratification, treatment options, recurrence prognosis, quality life. This scoping review maps existing literature on applications TC, particularly those leveraging clinical data, Electronic Medical Records (EMRs), synthesized findings. study analyzed 1231 papers, evaluated 203 full-text articles, selected 21 detailed three themes: (1) malignancy nodule classification; (2) other metastases derived TC prediction; (3) survival prediction. work examined case studies’ characteristics objectives identified key trends challenges ML-driven research. Finally, this addressed related highlighted directions to enhance domain while emphasizing its capability transform care into advanced precision medicine.

Language: Английский

Citations

0
Identification of oxidative stress-related subgroups and signature genes for the prediction of prognosis and immune microenvironment in thyroid cancer DOI Creative Commons

Zhiquan Qiu,

Jing Li,

Mei Tian

et al.

Molecular Genetics and Genomics, Journal Year: 2025, Volume and Issue: 300(1)

Published: April 30, 2025

Language: Английский

Citations

0
Advances in the Diagnosis and Treatment of Follicular Thyroid Carcinoma: A Comprehensive Review DOI Open Access

Vasundara Gopalan,

Swati G Deshpande,

Anup A Zade

et al.

Cureus, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 5, 2024

Follicular thyroid carcinoma (FTC) is the second most common type of cancer, presenting unique diagnostic and therapeutic challenges. This review provides a comprehensive analysis recent advancements in diagnosis treatment FTC, emphasizing significance these developments improving patient outcomes. We discuss evolution techniques, including imaging modalities, fine needle aspiration biopsy, molecular diagnostics, which have enhanced accuracy FTC detection differentiation from benign conditions. The also evaluates current strategies, surgical interventions, radioactive iodine therapy, targeted therapies, examining their effectiveness impact on prognosis. Additionally, we address ongoing challenges management, such as variability guidelines disparities care. Finally, explores emerging therapies future research directions, highlighting innovations that may further optimize management. By synthesizing knowledge identifying opportunities, this aims to contribute refining approaches for FTC.

Language: Английский

Citations

3
The Impact of NF-κB on Inflammatory and Angiogenic Processes in Age-Related Macular Degeneration DOI
Waleed Hassan Almalki, Salem Salman Almujri

Experimental Eye Research, Journal Year: 2024, Volume and Issue: 248, P. 110111 - 110111

Published: Sept. 24, 2024

Language: Английский

Citations

3
Editorial: Molecular targets in oncological and hematological disease management: innovations in precision medicine DOI Creative Commons
Adrian Bogdan Țigu,

Gregory Wiedman,

Stefan Eugen Szedlacsek

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 20, 2024

Language: Английский

Citations

0
DLK1 Is Associated with Stemness Phenotype in Medullary Thyroid Carcinoma Cell Lines DOI Open Access

Danilo Dias da Silva,

Rodrigo Pinheiro Araldi, Mariana Rocha Belizario

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 11924 - 11924

Published: Nov. 6, 2024

Medullary thyroid carcinoma (MTC) is a rare and aggressive tumor, often requiring systemic treatment in advanced or metastatic stages, where drug resistance presents significant challenge. Given the role of cancer stem cells (CSCs) recurrence resistance, we aimed to identify CSC subpopulations within two MTC cell lines harboring pathogenic variants most common MEN2-associated codons. We analyzed 15 stemness-associated markers, along with well-established markers (CD133, CD44, ALDH1), novel candidate (DLK1), multidrug proteins (MRP1 MRP3). The ability efflux fluorescent dye Hoechst 3342 form spheroids, representing behavior, was also assessed. MZ-CRC-1 (p.M918T) displayed higher expressions canonical DLK1, MRP than TT (p.C634W). formed more spheroids showed less accumulation (p < 0.0001). Finally, observed that DLK1+ (those expressing DLK1) both exhibited significantly levels stemness compared DLK1− lacking DLK1 expression). These findings underscore DLK1’s enhancing phenotype, providing valuable insights into progression suggesting potential therapeutic implications.

Language: Английский

Citations

0