Emerging treatment strategies in dry eye disease: potential of blood-derived approaches and natural plant-based products DOI
Lingli Kong,

Jiali Sun,

Razzagh Abedi-Firouzjah

et al.

Experimental Eye Research, Journal Year: 2024, Volume and Issue: unknown, P. 110217 - 110217

Published: Dec. 1, 2024

Language: Английский

The CD163 + tissue-infiltrating macrophages regulate ferroptosis in thyroid-associated ophthalmopathy orbital fibroblasts via the TGF-β/Smad2/3 signaling pathway DOI Creative Commons
Xuemei Li, Siyi Wang, Hui Cao

et al.

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: April 10, 2025

Thyroid-associated ophthalmopathy (TAO) is a thyroid function-related, organ-specific autoimmune disease that primarily leads to specific reactive changes and tissue remodeling in the periocular region. The exact pathogenesis of TAO remains unclear. High-throughput gene expression datasets related were comprehensively retrieved from Gene Expression Omnibus (GEO) database, selecting GSE174139 GSE158464 for analysis. Differentially expressed genes (DEGs) between patients healthy controls identified, ferroptosis-related (FRGs) obtained FerrDb database. intersection DEGs FRGs yielded associated with TAO.The transcriptional was validated using real-time quantitative polymerase chain reaction (RT-qPCR) on orbital adipose samples controls. Single-cell sequencing six human further analyzed cellular subpopulations within microenvironment.Additionally, co-culture model CD163 + macrophages fibroblasts, along an vitro TGF-β1-induced fibroblast (OF) model, constructed validate role TGF-β1/SMAD2/3 axis ferroptosis regulation. Finally, potential clinical drugs targeting high activity predicted Random Walk Restart (RWR) algorithm combined DGIdb We first utilized TAO-related GEO identify iron metabolism during progression through differential analysis, screening 7 key proteins. In validation revealed all but AOPQ LGMN, which upregulated, exhibited downregulated expression.Single-cell connective 4 2 identified 16,364 cells spanning 18 cell types. Analysis proteins fibroblasts displayed elevated signaling progression. Subcluster analysis distinct subpopulations, C2 subpopulation-characterized by CCL18-exhibiting prominent activation signals.Further samples, confirmed aberrant pathway as regulator ferroptosis. Hub subpopulation marker genes, macrophages. This study, integrating single-cell RNA-Seq bulk transcriptome involvement tissue-infiltrating regulating therapeutic candidates macrophage TAO. Furthermore, experiments demonstrated promotes highlighting novel intervention.

Language: Английский

Citations

0
Natural AI-based drug designing by modification of ascorbic acid and curcumin to combat buprofezin toxicity by using molecular dynamics study DOI Creative Commons
Haleema Sadia, Irfan Zia Qureshi, Muhammad Naveed

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 18, 2024

Language: Английский

Citations

2
Present Situation and Prospect of Prevention and Treatment of Mild Graves Ophthalmopathy DOI

卿龙 刘

Advances in Clinical Medicine, Journal Year: 2024, Volume and Issue: 14(11), P. 479 - 485

Published: Jan. 1, 2024

Language: Английский

Citations

0
Emerging treatment strategies in dry eye disease: potential of blood-derived approaches and natural plant-based products DOI
Lingli Kong,

Jiali Sun,

Razzagh Abedi-Firouzjah

et al.

Experimental Eye Research, Journal Year: 2024, Volume and Issue: unknown, P. 110217 - 110217

Published: Dec. 1, 2024

Language: Английский

Citations

0