Jan Sjödin faba bean mutation collection: status and molecular characterization DOI Creative Commons
Hamid Khazaei, Ulrika Carlson-Nilsson, Alan H. Schulman

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 28, 2024

Abstract Background Plant mutagenesis creates novel alleles that increase genetic diversity and enhance crop resilience, productivity, sustainability. The availability of the faba bean (Vicia faba L.) reference genome a growing set additional genomic resources has increased scientific practical value mutant collections. We phenotyped genotyped historical collection developed characterized by Jan Sjödin (1934–2023) over half century ago. was using high-throughput single-primer enrichment technology (SPET) assays. Results In this study, we used 11,073 informative single nucleotide polymorphism (SNP) markers spanning to genetically characterize 52 lines along with background line, cv. Primus. A range flowers, seeds, leaves, stipule mutations were observed. analysis population structure revealed shallow no major subpopulations. Principal component cluster analyses revealed, minor extent, mutants clustered their phenotype. Conclusions Given high marker density, our findings support power SPET for genotyping. Our results will be useful breeding studies in bean.

Language: Английский

Transforming field crops with CRISPR/Cas: a new era in genome editing DOI

Asif Mukhtiar,

Athar Mahmood, Muhammad Azam Khan

et al.

Rendiconti lincei. Scienze fisiche e naturali, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 7, 2025

Language: Английский

Citations

1
Twenty years of AT-HOOK MOTIF NUCLEAR LOCALIZED (AHL) gene family research – their potential in crop improvement DOI Creative Commons
Michaela Martinčová, Aleš Soukup

Current Plant Biology, Journal Year: 2025, Volume and Issue: unknown, P. 100460 - 100460

Published: Feb. 1, 2025

Language: Английский

Citations

1
Molecular tools for genome editing in plants: a synthetic overview DOI Creative Commons
Nicolau Brito da Cunha, Michel Lopes Leite, Júlio Carlyle Macedo Rodrigues

et al.

Vegetable Research, Journal Year: 2025, Volume and Issue: 5(1), P. 0 - 0

Published: Jan. 1, 2025

Language: Английский

Citations

1
Genome editing and kidney health DOI Creative Commons

Nadia Tavakolidakhrabadi,

Francesco Aulicino, Carl May

et al.

Clinical Kidney Journal, Journal Year: 2024, Volume and Issue: 17(5)

Published: April 17, 2024

ABSTRACT Genome editing technologies, clustered regularly interspaced short palindromic repeats (CRISPR)-Cas in particular, have revolutionized the field of genetic engineering, providing promising avenues for treating various diseases. Chronic kidney disease (CKD), a significant health concern affecting millions individuals worldwide, can arise from either monogenic or polygenic mutations. With recent advancements genomic sequencing, valuable insights into disease-causing mutations be obtained, allowing development new treatments these disorders. CRISPR-based emerged as potential therapies, especially diseases, offering ability to correct and eliminate phenotypes. Innovations genome led enhanced efficiency, specificity ease use, surpassing earlier tools such zinc-finger nucleases transcription activator-like effector (TALENs). Two prominent gene are prime base editing. Prime allows precise efficient modifications without inducing double-stranded DNA breaks (DSBs), while enables targeted changes individual nucleotides both RNA DNA, correction absence DSBs. These technologies treat diseases through specific mutations, somatic PKD1 PKD2 polycystic disease; NPHS1, NPHS2 TRPC6 focal segmental glomerulosclerosis; COL4A3, COL4A4 COL4A5 Alport syndrome; SLC3A1 SLC7A9 cystinuria even VHL renal cell carcinoma. Apart sequence, CRISPR-mediated epigenome offers cost-effective method treatment therapeutic development, given that epigenetic associated with However, there challenges overcome, including developing delivery methods, improving safety reducing off-target effects. Efforts improve CRISPR-Cas involve optimizing vectors, employing viral non-viral approaches minimizing immunogenicity. research animal models results rescuing expression wild-type podocin mouse nephrotic syndrome successful clinical trials early stages disorders, cancer immunotherapy, is hope translation

Language: Английский

Citations

7
Targeted genome editing for cotton improvement: prospects and challenges DOI
Rakesh Kumar, Joy Das,

Raghavendra Keelara Puttaswamy

et al.

The Nucleus, Journal Year: 2024, Volume and Issue: 67(1), P. 181 - 203

Published: March 21, 2024

Language: Английский

Citations

6
Molecular Communication of Microbial Plant Biostimulants in the Rhizosphere Under Abiotic Stress Conditions DOI Open Access
Sajid Ali, Muhammad Saeed Akhtar, Muhammad Siraj

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 12424 - 12424

Published: Nov. 19, 2024

Microbial plant biostimulants offer a promising, sustainable solution for enhancing growth and resilience, particularly under abiotic stress conditions such as drought, salinity, extreme temperatures, heavy metal toxicity. These biostimulants, including growth-promoting rhizobacteria, mycorrhizal fungi, nitrogen-fixing bacteria, enhance tolerance through mechanisms phytohormone production, nutrient solubilization, osmotic adjustment, antioxidant enzyme activation. Advances in genomics, metagenomics, transcriptomics, proteomics have significantly expanded our understanding of plant-microbe molecular communication the rhizosphere, revealing underlying these interactions that promote resilience. However, challenges inconsistent field performance, knowledge gaps stress-related signaling, regulatory hurdles continue to limit broader biostimulant adoption. Despite challenges, microbial hold significant potential advancing agricultural sustainability, amid climate change-induced stresses. Future studies innovation, Clustered Regularly Interspaced Short Palindromic Repeats other editing tools, should optimize formulations their application diverse agro-ecological systems. This review aims underscore current advances, future directions field, advocating multidisciplinary approach fully harness modern agriculture.

Language: Английский

Citations

5
Exploring RNAi’s Prospective Role in Curtailing Virus Transmission in Crop Plants DOI

Abreham Chebte Alemu

Published: Jan. 1, 2025

Language: Английский

Citations

0
Role of Biotechnology in Improvement and Regulation of Biosynthesis of Bioactive Compounds DOI
Sandeep Kour,

Tania Sagar,

Nipunta

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Genome Editing: An Approach to Engineer Crops to Withstand Climate Change and Sustain Food Security DOI
Othman Al‐Dossary

Published: Jan. 1, 2025

Language: Английский

Citations

0
Protocol Development for CRISPR/Cas9 Knockout of the Anti-inflammatory Protein TNIP1 in HaCaT Keratinocytes DOI
Liam E. Carman, Michael L. Samulevich, Brian J. Aneskievich

et al.

Methods in molecular biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0