Development,
Journal Year:
2022,
Volume and Issue:
149(21)
Published: Oct. 13, 2022
High-resolution
ribosome
fractionation
and
low-input
profiling
of
bovine
oocytes
preimplantation
embryos
has
enabled
us
to
define
the
translational
landscapes
early
embryo
development
at
an
unprecedented
level.
We
analyzed
transcriptome
polysome-
non-polysome-bound
RNA
profiles
(germinal
vesicle
metaphase
II
stages)
two-cell,
eight-cell,
morula
blastocyst
stages,
revealed
four
modes
selectivity:
(1)
selective
translation
non-abundant
mRNAs;
(2)
active,
but
modest
a
selection
highly
expressed
(3)
translationally
suppressed
abundant
moderately
(4)
mRNAs
associated
specifically
with
monosomes.
A
strong
low-abundance
transcripts
involved
in
metabolic
pathways
lysosomes
was
found
throughout
embryonic
development.
Notably,
genes
mitochondrial
function
were
prioritized
for
translation.
that
largely
reflected
transcription
two-cell
embryos,
observed
marked
shift
control
eight-cell
main
phase
genome
activation.
Subsequently,
become
more
synchronized
morulae
blastocysts.
Taken
together,
these
data
reveal
unique
spatiotemporal
regulation
accompanies
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(9), P. 4808 - 4808
Published: April 28, 2024
Extreme
temperature
during
summer
may
lead
to
heat
stress
in
cattle
and
compromise
their
productivity.
It
also
poses
detrimental
impacts
on
the
developmental
capacity
of
bovine
budding
oocytes,
which
halt
fertility.
To
mitigate
adverse
effects
stress,
it
is
necessary
investigate
mechanisms
through
affects
oocytes.
The
primary
goal
this
study
was
impact
epigenetic
modifications
oocytes
embryos,
as
well
oocyte
capacity,
reactive
oxygen
species,
mitochondrial
membrane
potential,
apoptosis,
transzonal
projections,
gene
expression
levels.
Our
results
showed
that
significantly
reduced
levels
from
histone
H1,
H2A,
H2B,
H4,
DNA
methylation,
hydroxymethylation
at
all
stages
embryo.
Similarly,
cleavage
rate,
blastocyst
mitochondrial-membrane
potential
level,
adenosine-triphosphate
(ATP)
copy
number,
projection
level.
found
affected
distribution
increased
apoptosis
autophagy
findings
suggest
genes
related
ability,
cytoskeleton,
function,
modification,
lowering
competence
season.
Cells,
Journal Year:
2023,
Volume and Issue:
12(14), P. 1874 - 1874
Published: July 17, 2023
In
this
review,
advances
in
the
understanding
of
epigenetic
reprogramming
from
fertilization
to
development
primordial
germline
cells
a
mouse
and
human
embryo
are
discussed.
To
gain
insights
into
molecular
underpinnings
various
diseases,
it
is
essential
comprehend
intricate
interplay
between
genetic,
epigenetic,
environmental
factors
during
cellular
embryonic
differentiation.
An
increasing
range
including
cancer
developmental
disorders,
have
been
linked
alterations
DNA
methylation
histone
modifications.
Global
occurs
mammals
at
two
stages:
post-fertilization
germ
(PGC).
Epigenetic
after
involves
rapid
demethylation
paternal
genome
mediated
through
active
passive
demethylation,
gradual
maternal
demethylation.
The
de
novo
methyltransferase
enzymes,
Dnmt3a
Dnmt3b,
restore
beginning
blastocyst
stage
until
formation
gastrula,
maintenance
methyltransferase,
Dnmt1,
maintains
somatic
cells.
PGC
undergo
second
round
global
allocation
formative
pluripotent
before
gastrulation,
where
imprints
marks
on
transposable
elements
known
as
retrotransposons,
long
interspersed
nuclear
(LINE-1)
intracisternal
A-particle
(IAP)
demethylated
well.
Finally,
restored
implantation
sex-specific
corresponding
sex
embryo.
This
review
introduces
novel
perspective
by
uncovering
how
toxicants
stress
stimuli
impact
critical
period
pluripotency,
potentially
influencing
both
quantity
quality
PGCs.
Furthermore,
comprehensive
comparison
events
mice
humans
breaks
new
ground,
empowering
researchers
make
informed
decisions
regarding
suitability
models
for
their
experiments.
Biology of Reproduction,
Journal Year:
2024,
Volume and Issue:
unknown
Published: June 8, 2024
Choline
is
a
vital
micronutrient
that
can
be
utilized
in
the
formation
of
betaine
and
multiple
phospholipids.
In
this
study,
we
aimed
to
confirm,
expand
on
previous
findings,
how
choline
impacts
embryos
from
first
7
days
development
affect
postnatal
phenotype.
Bos
indicus
were
cultured
choline-free
medium
(termed
vehicle)
or
supplemented
with
1.8
mM
Blastocyst-stage
transferred
into
crossbred
recipients.
Once
born,
calves
evaluated
at
birth,
94
d,
178
d
weaning
(average
age
=
239
d).
Following
weaning,
all
enrolled
feed
efficiency
trial
before
being
separated
by
sex,
males
slaughtered
approximately
580
females
followed
until
their
pregnancy
check.
Results
confirm
exposure
chloride
during
alters
characteristics
resultant
calves.
Calves
both
sexes
choline-treated
consistently
heavier
through
had
testes
3
mo
age.
There
sex-dependent
alterations
DNA
methylation
whole
blood
caused
treatment.
After
was
affected
an
interaction
more
efficient
for
less
males.
heavier,
tended
than
vehicle
observations
after
weaning.
Carcass
weight
cross-sectional
area
Longissumus
thoracis
muscle
increased
choline.
Few
became
pregnant
experiment
although
numerically
females.
preimplantation
embryo
alter
phenotypes
19
months
birth.
International Journal of Molecular Sciences,
Journal Year:
2021,
Volume and Issue:
22(21), P. 11668 - 11668
Published: Oct. 28, 2021
Assisted
reproductive
techniques
(ART)
and
parental
nutritional
status
have
profound
effects
on
embryonic/fetal
placental
development,
which
are
probably
mediated
via
"programming"
of
gene
expression,
as
reflected
by
changes
in
their
epigenetic
landscape.
Such
may
underlie
programming
growth,
function
fetal
organs
later
pregnancy
the
offspring
postnatally,
potentially
lead
to
long-term
organ
structure
adults.
This
latter
concept
has
been
termed
developmental
origins
health
disease
(DOHaD),
or
simply
programming,
emerged
a
major
issue
animals
humans
because
it
is
associated
with
an
increased
risk
non-communicable
diseases
offspring,
including
metabolic,
behavioral,
dysfunction.
In
this
review,
we
will
briefly
introduce
its
relationship
epigenetics.
We
then
discuss
evidence
that
ART
periconceptual
maternal
paternal
nutrition
alterations
very
early
pregnancy,
how
each
experiences
based
signals
received
from
dam.
Lastly,
current
research
strategies
designed
overcome
minimize
negative
consequences
or,
conversely,
maximize
positive
aspects
programming.
Reproduction Fertility and Development,
Journal Year:
2021,
Volume and Issue:
34(2), P. 36 - 51
Published: Oct. 28, 2021
Genomic
selection
combined
with
in
vitro
embryo
production
(IVEP)
oocytes
from
heifer
calves
provides
a
powerful
technology
platform
to
reduce
generation
interval
and
significantly
increase
the
rate
of
genetic
gain
cattle.
The
ability
obtain
developmental
competence
has
been
recognised
for
more
than
60years.
There
is
renewed
interest
potential
this
reproductive
as
beef
dairy
industries
seek
identify
multiply
animals
that
satisfy
consumer
demand
efficient
utilisation
natural
resources,
minimal
environmental
impact
high
product
quality.
Heifer
show
good
follicular
response
FSH
stimulation
can
be
recovered
by
ovum
pick-up
(OPU).
However,
efficiency
OPU/IVEP
remains
lower
compared
peripubertal
heifers
cows,
both
indicus
(Zebu,
Bos
)
taurus
(Bos
breeds.
Indicus
cattle
generally
have
follicles,
yield
greater
number
oocytes,
better
IVEP
outcome,
treatment
prepubertal
bovine
somatotrophin
(bST)
propylene
glycol
before
yet
major
improvement
production.
Holstein
(taurus)
derived
different
technologies
(AI,
MOET,
OPU/IVEP)
showed
similar
age
at
puberty
first-lactation
milk
embryos
transferred
or
cows
likewise
yielded
same
performance.
gains
productivity
achieved
strategic
use
make
relevant
highly
important
breeding.
Ongoing
optimisation
needed
young
donors
fully
realised.
Epigenetics Insights,
Journal Year:
2023,
Volume and Issue:
16
Published: Jan. 1, 2023
Background:
Alterations
in
DNA
methylation
play
an
important
role
cancer
development
and
progression.
Dietary
nutrients
lifestyle
behaviors
can
influence
patterns
thereby
modulate
risk.
Introduction:
To
comprehensively
review
available
evidence
on
how
dietary
factors
impact
contribute
to
carcinogenesis
through
epigenetic
mechanisms.
Materials
methods:
A
literature
search
was
conducted
using
PubMed
identify
relevant
studies
published
between
2005
2022
that
examined
relationships
dietary/lifestyle
cancer.
Studies
investigating
the
effects
of
components
(eg,
micronutrients,
phytochemicals),
physical
activity,
smoking,
obesity
global
gene-specific
changes
animal
human
models
were
included.
Data
specific
exposures,
types,
targets
underlying
mechanisms
extracted.
Results:
Multiple
found
methyltransferase
methyl
donor
availability,
generation
oxidative
stress.
Altered
genes
regulating
cell
proliferation,
apoptosis,
inflammation
linked
Conclusion:
interventions
aimed
at
modulating
have
potential
for
both
prevention
treatment
Further
research
is
needed
actionable
nutrition
lifestyle-based
therapies.
Animal Reproduction Science,
Journal Year:
2024,
Volume and Issue:
269, P. 107476 - 107476
Published: April 18, 2024
Despite
decades
of
research
and
handling
semen
for
use
in
artificial
insemination
(AI)
other
assisted
reproductive
technologies,
5-10%
selected
boar
sires
are
still
considered
sub-fertile,
escaping
current
assessment
methods
sperm
quality
resilience
to
preservation.
As
end-product,
the
ejaculate
(emitted
spermatozoa
sequentially
exposed
composite
seminal
plasma,
SP)
ought
define
homeostasis
testes,
epididymis,
accessory
sexual
glands.
Yet,
linking
findings
production
biology
fertility
is
suboptimal.
The
present
essay
critically
reviews
how
a
fertile
can
help
us
diagnose
both
health
handling,
focusing
on
-available
under
development-
identify
suitable
biomarkers
cryotolerance
fertility.
Bulk
SP,
proteins
microRNAs
(miRNAs)
have,
albeit
linked
function
after
AI,
failed
enhance
outcomes
at
commercial
level,
perhaps
just
being
components
complex
functional
pathway.
Hence,
focus
now
interaction
sperm-SP,
comparing
vivo
with
ex
vivo,
regarding
nano-sized
lipid
bilayer
extracellular
vesicles
(sEVs)
as
priority.
sEVs
transport
fragile
molecules
(lipids,
proteins,
nucleic
acids)
which,
shielded
from
degradation,
mediate
cell-to-cell
communication
female
internal
genital
tract.
Such
modulates
essential
processes,
immunological
tolerance.
be
harvested,
characterized,
stored,
manipulated,
e.g.
used
andrological
diagnosis,
selection
breeders,
alternatively
additives
improve
cryosurvival
Animals,
Journal Year:
2025,
Volume and Issue:
15(2), P. 189 - 189
Published: Jan. 12, 2025
Modern
hyperprolific
sows
are
increasingly
susceptible
to
health
challenges.
Their
rapid
growth
rates
predispose
them
locomotor
disorders,
while
high
metabolic
demands,
reduced
backfat
thickness,
and
increased
protein
accretion
heighten
their
vulnerability
heat
stress
dystocia.
Additionally,
prolonged
farrowing
negatively
affects
the
oxidative
inflammatory
status
of
these
females.
prevalent
conditions
such
as
gastric
ulcers
cystitis
raise
ethical,
welfare,
economic
concerns.
Despite
several
studies
related
sow
nutrition,
there
no
which
compile
extrapolate
nutrition
approaches
from
rearing
period
impact
on
sows’
longevity.
Also,
aim
our
review
was
shed
light
gaps
that
require
further
investigation.
Controlling
body
condition
scores
is
crucial
for
maximizing
productivity
in
sows.
During
gestation,
high-fiber
diets
help
maintain
optimal
prevent
constipation,
particularly
during
peripartum
period.
Antioxidants
offer
a
range
beneficial
effects
this
critical
phase.
probiotics
acidifiers
can
enhance
gut
lower
risk
genitourinary
infections.
On
day
farrowing,
energy
supplementation
emerges
promising
strategy
reduce
duration.
Collectively,
strategies
address
major
challenges,
enhancing
welfare
promoting
sow’s
