Published: Jan. 1, 2024
Language: Английский
Frontiers in Nutrition, Journal Year: 2023, Volume and Issue: 10
Published: Feb. 10, 2023
Biofortification is gaining importance globally to improve human nutrition through enhancing the micronutrient content, such as vitamin A, iron, and zinc, in staple food crops. The present study aims identify chromosomal regions governing grain iron concentration (GFeC), zinc (GZnC), thousand kernel weight (TKW) using recombinant inbred lines (RILs) wheat, developed from a cross between HD3086 HI1500. experiment was conducted four different production conditions at Delhi viz. , control, drought, heat, combined heat drought stress Indore under stress. Grain content increased conditions, while decreased. Medium high heritability with moderate correlation observed. Out of 4,106 polymorphic markers parents, 3,407 SNP were used for linkage map construction which spanned over length 14791.18 cm. QTL analysis identified total 32 traits study, includes 9, 11, 12 QTLs GFeC, GZnC, TKW, respectively. A hotspot on chromosome 4B associated explaining phenotypic variance 29.28, 10.98, 17.53%, Similarly, common loci chromosomes 4D weight. In silico these putative candidate genes that code proteins Inositol 1,3,4-trisphosphate 5/6-kinase, P-loop containing nucleoside triphosphate hydrolase, Pleckstrin homology (PH) domains, Serine-threonine/tyrosine-protein kinase F-box-like domain superfamily play role many important biochemical or physiological process. linked can be MAS once successfully validated.
Language: Английский
Citations
12
Genes, Journal Year: 2023, Volume and Issue: 14(1), P. 221 - 221
Published: Jan. 14, 2023
A set of 188 recombinant inbred lines (RILs) derived from a cross between high-yielding Indian bread wheat cultivar HD2932 and synthetic hexaploid (SHW) Synthetic 46 tetraploid Triticum turgidum (AA, BB 2n = 28) diploid tauschii (DD, 14) was used to identify novel genomic regions associated in the expression grain iron concentration (GFeC), zinc (GZnC), protein content (GPC) thousand kernel weight (TKW). The RIL population genotyped using SNPs 35K Axiom® Wheat Breeder’s Array 34 SSRs phenotyped two environments. total nine QTLs including five for GPC (QGpc.iari_1B, QGpc.iari_4A, QGpc.iari_4B, QGpc.iari_5D, QGpc.iari_6B), GFeC (QGfec.iari_5B QGfec.iari_6B), one each GZnC (QGznc.iari_7A) TKW (QTkw.iari_4B) were identified. stable co-localized (QGpc.iari_4B QTkw.iari_4B) identified on 4B chromosome flanking region Xgwm149–AX-94559916. In silico analysis revealed that key putative candidate genes such as P-loop containing nucleoside triphosphatehydrolase, Nodulin-like protein, NAC domain, Purine permease, Zinc-binding ribosomal Cytochrome P450, Protein phosphatase 2A, Zinc finger CCCH-type, Kinesin motor domain located within QTL these are involved regulation homeostasis, transportation, Fe, Zn, remobilization developing grain, size shape, increased nitrogen use efficiency. QTLs, particularly useful subsequent marker-assisted selection (MAS).
Language: Английский
Citations
11
Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16
Published: April 24, 2025
Food and nutritional security are global challenges exacerbated by an increasing human population impacted climate change. Barley is among the top cereal crops grown worldwide a strategic crop for food nutrition in several geographical domains. However, barley grains generally limited iron zinc, two major micronutrient deficiencies affecting billions of people around world, but particularly women children developing countries. One promising strategy to enhance status via biofortification, identification use nutrient-rich natural variants genetic improvement. Germplasm assessed as being rich essential nutrients used parental materials traditional breeding strategies. While simple theory, directly assessing grain nutrient concentration phenotype program not trivial, lesser developed geographies. As alternative, diagnostics can simplify desirable progenies accelerate process. Here we explored variation zinc within 296 Ethiopian Eritrean landraces using genome-wide association study. We found strong associations with SNPs, both which were located ortholog tonoplast-associated facilitator superfamily (MFS) transporter gene, Zinc induced facilitator-like 1 (ZIFL1) Arabidopsis thaliana (AtZIFL1). Sequence-based haplotype analysis gene (HvZIFL1) extended this 153-162 bp deletion non-coding region. The favourable haplotype, associated higher Zn concentration, was ~20% germplasm. Markers designed diagnostic SNPs molecular genotypes enhanced Zn.
Language: Английский
Citations
0
Field Crops Research, Journal Year: 2023, Volume and Issue: 292, P. 108826 - 108826
Published: Jan. 21, 2023
Language: Английский
Citations
9
BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)
Published: July 15, 2024
Biofortification represents a promising and sustainable strategy for mitigating global nutrient deficiencies. However, its successful implementation poses significant challenges. Among staple crops, wheat emerges as prime candidate to address these nutritional gaps. Wheat biofortification offers robust approach enhance cultivars by elevating the micronutrient levels in grains, addressing one of most crucial concerns present era.
Language: Английский
Citations
3
Molecular Genetics and Genomics, Journal Year: 2023, Volume and Issue: 298(6), P. 1515 - 1526
Published: Oct. 18, 2023
Abstract Globally, over 2 billion people suffer from malnutrition due to inadequate intake of micronutrients. Genomic-assisted breeding is identified as a valuable method facilitate developing new improved plant varieties targeting grain yield and micronutrient-related traits. In this study, genome-wide association study (GWAS) single- multi-trait-based genomic prediction (GP) analysis was conducted using set 252 elite wheat genotypes the International Center for Agricultural Research in Dry Areas (ICARDA). The objective identify linked SNP markers, putative candidate genes evaluate estimated values (GEBVs) traits.. For purpose, field trial at drought-prone station, Merchouch, Morocco consecutive years (2018 2019) followed by GWAS with 10,173 quality markers. studied exhibited significant genotypic variation highly significantly associated markers on chromosomes 1B 2B zinc (Zn) iron (Fe) contents, respectively. predictive ability selenium (Se) Fe traits GP GBLUP model 0.161 0.259 improving 6.62 4.44%, respectively , compared corresponding single-trait-based models. genes, developed models could potentially programs improve overall genetic gain biofortification micronutrients via marker-assisted (MAS) selection (GS) methods.
Language: Английский
Citations
7
Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 13
Published: Jan. 16, 2023
Wheat is grown and consumed worldwide, making it an important staple food crop for both its calorific nutritional content. In places where wheat used as a food, suboptimal micronutrient content levels, especially of grain iron (Fe) zinc (Zn), can lead to malnutrition. Grain nutrient influenced by abiotic stresses, such drought heat stress. The best method addressing deficiencies the biofortification crops. prerequisites marker-assisted varietal development are identification genomic region responsible high contents understanding their genetics.A total 193 diverse genotypes were evaluated under stress conditions across years at Indian Agricultural Research Institute (IARI), New Delhi, timely sown irrigated (IR), restricted (RI) late (LS) conditions. (GFeC) (GZnC) estimated from control treatment groups. Genotyping all lines study was carried out with single nucleotide polymorphisms (SNPs) Breeder's 35K Axiom Array.Three subgroups observed in association panel based on principal component analysis (PCA) dendrogram analysis. A large whole-genome linkage disequilibrium (LD) block size 3.49 Mb observed. genome-wide identified 16 unique stringent marker trait associations GFeC, GZnC, 1000-grain weight (TGW). silico demonstrated presence 28 potential candidate genes flanking linked SNPs, synaptotagmin-like mitochondrial-lipid-binding domain, HAUS augmin-like complex, di-copper center-containing protein kinase, chaperonin Cpn60, finger, NUDIX hydrolase, etc. Expression levels these vegetative tissues also found. Utilization markers breeding may rapid biofortified combat
Language: Английский
Citations
6
Crop Design, Journal Year: 2024, Volume and Issue: unknown, P. 100084 - 100084
Published: Oct. 1, 2024
Language: Английский
Citations
2
International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(17), P. 13304 - 13304
Published: Aug. 27, 2023
The content and quality of gluten in wheat grain is a distinctive characteristic that determines the final properties flour. In this study, genome-wide association study (GWAS) was performed on panel consisting bread varieties introgression lines (ILs) obtained via hybridization with tetraploid relatives. A total 17 stable quantitative trait nucleotides (QTNs) located chromosomes 1D, 2A, 2B, 3D, 5A, 6A, 7B, 7D explained up to 21% phenotypic variation were identified. Among them, QTLs 2A 7B found contain three six linked SNP markers, respectively. Comparative analysis genotypes according composition haplotypes for closely SNPs chromosome indicated haplotype TT/AA/GG ten ILs containing introgressions from T. timopheevii. plants significantly higher than GG/GG/AA. Having compared newly data previously reported loci (QTLs) we inferred locus inherited timopheevii potentially novel. new can be used as sources genetic factors improve traits wheat.
Language: Английский
Citations
4
Cogent Food & Agriculture, Journal Year: 2024, Volume and Issue: 10(1)
Published: May 31, 2024
There are about two billion people suffered malnourished because of the failing global food system, historical emphasis on crop yield, notably lacking in Fe and Zn. Breeding biofortified staple crops is a sustainable strategy to tackle this issue, but it poses challenges, primarily due limited genetic resources modern breeding populations. Simultaneously, climate change, marked by rising temperatures increased atmospheric CO2, threatens stability micronutrient concentrations harvest organs, such as gain, fruit, root, tubers etc. The rich biodiversity wild relatives, including species, landraces, natives, offers promising response change. Advanced technologies, like marker-assisted selection breeding, genomic selection, gene editing provide opportunities enhance Zn crops, rice, maize, wheat, cassava, Genome-wide association studies population genetics research will help found qualitative genes major-effect homeostasis for Zn, which purpose uncover molecular mechanisms governing regulation crops. Despite these strides, enhancing bioavailability human, requires considering absorption enhancers inhibitors, rather than solely increasing concentrations. Absorption testing systems Caco-2 cell line, elucidating meta-process human body should be considered when through traditional breeding.
Language: Английский
Citations
0