Gene expression variation underlying tissue-specific responses to copper stress inDrosophila melanogaster DOI Creative Commons
Elizabeth R. Everman, Stuart J. Macdonald

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: July 12, 2023

Copper is one of a handful biologically necessary heavy metals that also common environmental pollutant. Under normal conditions, copper ions are required for many key physiological processes. However, in excess, quickly results cell and tissue damage can range severity from temporary injury to permanent neurological damage. Because its biological relevance, because conserved copper-responsive genes respond other non-essential metal pollutants, resistance

Language: Английский

Identification of a longevity gene through evolutionary rate covariation of insect mito-nuclear genomes DOI

Mei Tao,

Jiani Chen, Chunlai Cui

et al.

Nature Aging, Journal Year: 2024, Volume and Issue: 4(8), P. 1076 - 1088

Published: June 4, 2024

Language: Английский

Citations

8
Independently evolved pollution resistance in four killifish populations is largely explained by few variants of large effect DOI
Jeffrey T. Miller, Bryan W. Clark, Noah M. Reid

et al.

Evolutionary Applications, Journal Year: 2024, Volume and Issue: 17(1)

Published: Jan. 1, 2024

Abstract The genetic architecture of phenotypic traits can affect the mode and tempo trait evolution. Human‐altered environments impose strong natural selection, where successful evolutionary adaptation requires swift large shifts. In these scenarios, theory predicts that is due to a few adaptive variants effect, but empirical studies have revealed rapidly evolved phenotypes are rare, especially for populations inhabiting polluted environments. Fundulus killifish repeatedly resistance extreme pollution in urban estuaries. Prior studies, including genome scans signatures some genes pathways important resistance, provide context genotype–phenotype association reported here. We created multiple quantitative locus (QTL) mapping families using progenitors from four different resistant populations, RAD‐seq genetically mapped variation sensitivity (developmental perturbations) following embryonic exposure model toxicant PCB‐126. found one two large‐effect QTL loci accounted PCB‐mediated developmental toxicity. QTLs harbored candidate govern regulation aryl hydrocarbon receptor (AHR) signaling. One was shared across all another three populations. showed recent selection corresponding wild population did not. Some PCB inferred were identified as QTL, key conclude defects normally caused by PCB‐126 governed effect. However, other aspects beyond may be additional loci, such comprehensive PCB‐126, mixtures chemicals distinguish estuaries more broadly, complex.

Language: Английский

Citations

4
Lead (Pb) concentrations across 22 species of butterflies correlate with soil and air lead and decreased wing size in an urban field study DOI Creative Commons
Lindsey R. Kemmerling, Ashley L. Darst,

Mina Adabag

et al.

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 969, P. 178900 - 178900

Published: March 1, 2025

Pollution is a global issue contributing to biodiversity loss, climate change, and human health concerns. Lead (Pb) has long been recognized as toxic heavy metal pollutant but few studies have investigated the impact routes of exposure lead in field conditions across multiple species. We collected 22 common species butterflies gradient pollution Twin Cities metropolitan area (Minneapolis St. Paul, MN, USA). measured their thorax concentrations body condition including wing area, number eggs, brain mass. quantified soil, host plant leaves, air (through lichen bio-monitors) at sites where were investigate potential exposure. found negative correlation between sublethal butterfly size all Contrary expectations from previous literature, we did not find correlations concentration eggs or Our data indicate that for are particularly pronounced through soil air, relative plants, there positive nearby plants. Such effects lead, even low levels pollution, underline importance continuing reduce emissions impacts pollutants protect biodiversity.

Language: Английский

Citations

0
Application of the Drosophila melanogaster Research Model to Evaluate the Toxicity Levels between Lead and Copper DOI Creative Commons

Sărac Ioan,

Petrescu Irina,

Onisan Emilian

et al.

Applied Sciences, Journal Year: 2024, Volume and Issue: 14(10), P. 4190 - 4190

Published: May 15, 2024

Applications of the Drosophila melanogaster (D.m.) research model have an important means both for genetic investigations and study metal toxicity, because D.m. has physiological mechanisms comparable to those in human organisms. In this research, toxic effect lead (Pb2+) copper (Cu2+) on four genotypes—the wild genotype (Oregon-R, used as control) three mutant genotypes (white, brown, white-vestigial)—was compared. Five replicates were made observe development progress monitorized exposed five different concentrations 0, 0.50, 0.75, 1.00, 2.00 mM (CuSO4) Pb(C2H3O2)2. Proliferation rates larvae, pupae, adults depend factors, metals (copper or lead), their concentrations. The white-vestigial showed greatest sensitivity at IC50 concentration (inhibition proliferation more than 50% compared with control sample) doses 1.00 Cu2+ Pb2+. contrast, (Oregon-R) only inhibition Cu2+. results conclude that (i) dose influences prolificacy rate a directly proportional way, (ii) comparative analyses between Pb2+ revealed acute Cu2+, (iii) differentiated values according recorded. Those reflect importance using studies interactions factors toxicity. Also, provides significant information non-toxic maximum

Language: Английский

Citations

2
Gene expression variation underlying tissue-specific responses to copper stress in Drosophila melanogaster DOI Creative Commons
Elizabeth R. Everman, Stuart J. Macdonald

G3 Genes Genomes Genetics, Journal Year: 2024, Volume and Issue: 14(3)

Published: Jan. 23, 2024

Abstract Copper is one of a handful biologically necessary heavy metals that also common environmental pollutant. Under normal conditions, copper ions are required for many key physiological processes. However, in excess, results cell and tissue damage ranging severity from temporary injury to permanent neurological damage. Because its biological relevance, because conserved copper-responsive genes respond nonessential metal pollutants, resistance Drosophila melanogaster useful model system with which investigate the genetic control stress response. toxicity has potential differently impact specific tissues, we genetically characterized gene expression response tissue-specific manner this study. We assessed head gut 96 inbred strains Synthetic Population Resource using combination differential analysis quantitative trait locus mapping. Differential revealed clear patterns expression. Tissue treatment responses were detected Expression associated MtnA, Mdr49, Mdr50, Sod3 exhibited both genotype-by-tissue genotype-by-treatment effects on under stress, illuminating tissue- treatment-specific control. Together, our data build nuanced description roles interactions between allelic variation genes, provide valuable insight into genomic architecture susceptibility toxicity, highlight candidate future functional characterization.

Language: Английский

Citations

1
Drosophila melanogaster Modelinde Maleik Asit ve Vanilik Asitin Bakır Toksisitesi Üzerine Etkilerinin Araştırılması DOI
Emine Toraman, Melike Karaman

Türk doğa ve fen dergisi :/Türk doğa ve fen dergisi, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 6, 2024

Bakır, tüm canlı organizmaların biyolojik fonksiyonlarının sürdürülmesi için gerekli olan bir metaldir. düşük konsantrasyonlarda hücresel metabolizmanın olmasına rağmen, yüksek ROS oluşumuna neden olduğundan toksik etkilere olabilir. Bu çalışmada larva ve yetişkin D. melanogaster'de CuSO4 (1 mM) ile toksisite oluşturuldu. Sinekler daha sonra maleik asit (MA) (2 mg) vanilik (VA) işlendi. Sonuçlar Cu toksisitesinin SOD, CAT, GPX, AChE GSH düzeylerinde azalmaya olduğunu gösterdi. MDA önemli artış oldu. Ancak MA VA tedavisinin miktarlarını artırdığı, miktarını ise azalttığı belirlendi. sonuçlar VA'nın CuSO4'ün olduğu oksidatif stres üzerinde iyileştirici sahip Sonuç olarak, gelecekteki çalışmalarda metal kaynaklı toksisiteye karşı doğal bileşiklerin farklı parametreler üzerindeki etkileri değerlendirilmelidir.

Citations

0
Gene expression variation underlying tissue-specific responses to copper stress inDrosophila melanogaster DOI Creative Commons
Elizabeth R. Everman, Stuart J. Macdonald

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: July 12, 2023

Copper is one of a handful biologically necessary heavy metals that also common environmental pollutant. Under normal conditions, copper ions are required for many key physiological processes. However, in excess, quickly results cell and tissue damage can range severity from temporary injury to permanent neurological damage. Because its biological relevance, because conserved copper-responsive genes respond other non-essential metal pollutants, resistance

Language: Английский

Citations

0