Genome wide identification of Dof transcription factors in Carmine radish reveals RsDof33 role in cadmium stress and anthocyanin biosynthesis

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 8, 2025

Language: Английский

Genome-Based Identification of the Dof Gene Family in Three Cymbidium Species and Their Responses to Heat Stress in Cymbidium goeringii

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(14), P. 7662 - 7662

Published: July 12, 2024

As an important genus in Orchidaceae, Cymbidium has rich ecological diversity and significant economic value. DNA binding with one zinc finger (Dof) proteins are pivotal plant-specific transcription factors that play crucial roles the growth, development, stress response of plants. Although Dof genes have been identified functionally analyzed numerous plants, exploration Orchidaceae remains limited. We conducted a thorough analysis gene family goeringii, C. ensifolium, sinensis. In total, 91 (27 CgDofs, 34 CeDofs, 30 CsDofs) were identified, divided into five groups (I–V) based on phylogenetic analysis. All motif 1 2 conserved domains over half contained introns. Chromosomal localization collinearity revealed their evolutionary relationships potential duplication events. Analysis cis-elements CsDofs promoters showed light-responsive most common, followed by hormone-responsive elements, plant growth-related abiotic elements. three species primarily exhibit random coil structure, while homology modeling exhibited similarity. addition, RT-qPCR expression levels nine CgDofs changed greatly under heat stress. CgDof03, CgDof22, CgDof27, CgDof08, CgDof23 varying degrees upregulation. Most upregulated belong to group I, indicating I great for high-temperature resistance. conclusion, our study systematically demonstrated molecular characteristics different species, preliminarily patterns stress, provided reference further breeding orchids.

Language: Английский

Plant AT-rich protein and zinc-binding protein (PLATZ) family in Dendrobium huoshanense: identification, evolution and expression analysis

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Dec. 30, 2024

PLATZ (plant A/T-rich protein and zinc-binding protein) transcription factors are essential for plant growth, development, responses to abiotic stress. The regulatory role of genes in the environmental adaptation D. huoshanense is inadequately comprehended. genome-wide identification elucidates functions processes gene family. Our investigation encompassed examination structures chromosome distribution, construction phylogenetic tree with its relatives, analysis cis-acting elements expression profiles potentially implicated growth stress responses. Eleven DhPLATZs were classified into three clades (I, II, III) according their evolutionary homology. distribution these over six chromosomes indicated that both whole genome duplication (WGD) segmental events have contributed expansion this Ka/Ks revealed a pattern purifying selection after occurrences, suggesting little alterations functional divergence. collinearity microsynteny results DhPLATZ shared same conserved domains as paralogs from chrysotoxum. Expression profiling quantitative demonstrated had unique patterns response phytohormones cold Subcellular localization expressed nucleus, factors. These findings enhance our understanding genes' involvement species underscore significance important areas further research.

Language: Английский