Normal butanol fraction of Polygonum hydropiper L. flavonoids reduces inflammation caused by PCV2 infections in cell and mouse models DOI Creative Commons

Yuheng Wei,

Shu‐Mian Zhou,

Wen Zhao

et al.

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12

Published: Feb. 4, 2025

Introduction The normal butanol fraction of Polygonum hydropiper L. flavonoids (FNB) exhibits significant anti-inflammatory effects. This study investigated FNB's impact on inflammatory responses induced by Porcine circovirus type 2 (PCV2) in cell and mouse models. Methods An model was established RAW264.7 cells infected with varying PCV2 concentrations. And assigning both 108 SPF-grade KM mice to Control, PCV2, Rutin, various dosages FNB groups. Inflammatory factors such as Monocyte Chemoattractant Protein-1 (MCP-1), interleukin-6 (IL-6), IL-8, IL-10, Tumor Necrosis Factor-alpha (TNF-α), Reactive Oxygen Species (ROS), Nitric Oxide (NO) were quantified using ELISA, RT-qPCR immunohistochemistry. Results showed that a titer 10 4.5 TCID 50 /0.1 mL when applied effectively an vitro at 12 24 h post-infection. Following infection, all the displayed increased culture supernatant intracellular mRNA expression levels ( p < 0.05 or 0.01), but these reduced treatment 0.01). In sera post-PCV2 infection also elevated IL-6, IL-8 TNF-α, MCP-1 Additionally, protein for iNOS rose significantly lung tissues 0.01) decreased Discussion These findings suggest reduces factor production modulates response triggered potentially enhancing host resistance against it.

Language: Английский

In vitro study of cold atmospheric plasma-activated liquids inhibits malignant melanoma by affecting macrophage polarization through the ROS/JAK2/STAT1 pathway DOI Open Access
Cheng Chen,

Shiyun Zhou,

Xingyu Yang

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 175, P. 116657 - 116657

Published: April 30, 2024

Melanoma is a prevalent malignant skin tumor known for its high invasive ability and rate of metastasis, making clinical treatment exceptionally challenging. Tumor-associated macrophages (TAMs) are the most abundant immune cells in microenvironment play crucial role survival development. Cold atmospheric plasma (CAP) an emerging tool that has garnered attention from scholars due to interaction with non-tumor microenvironment. Here, we used macrophage lines THP-1 RAW264.7, as well melanoma cell A375 MV3, research subjects investigate effect plasma-activated liquid (PAL) on differentiation inhibitory proliferation. We confirmed killing PAL was selective. Using flow cytometry PCR, discovered can influence differentiation. Through vitro coculture, demonstrated PAL-treated significantly impede development progression, more potent than directly targeting cells. Furthermore, have proposed hypothesis promotes into M1 type through ROS/JAK2/STAT1 pathway. To test hypothesis, employed catalase fludarabine block different sites The results were then validated Western Blot, qPCR ELISA. This study illustrates therapy effective immunotherapy expands scope immunotherapy. these findings establish theoretical foundation potential applications PAL.

Language: Английский

Citations

6
A mussel-inspired, antibacterial, antioxidant, injectable composite hydrogel for the sustain delivery of salvianolic acid B for the treatment of frozen shoulder DOI Creative Commons
Yan Yan,

Xinhao Li,

Chen Chen

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 40, P. 396 - 416

Published: June 19, 2024

Language: Английский

Citations

6
miR‐3184‐3p enriched in cerebrospinal fluid exosomes contributes to progression of glioma and promotes M2‐like macrophage polarization DOI Creative Commons
Hao Xu, Ming Li, Ziwen Pan

et al.

Cancer Science, Journal Year: 2022, Volume and Issue: 113(8), P. 2668 - 2680

Published: April 12, 2022

Abstract Liquid biopsy is a novel strategy for tumour diagnosis. The contents of cerebrospinal fluid (CSF) exosomes could reflect glioma status, hence sampling from CSF means liquid glioma. However, few studies have focused on the function microRNAs in exosomes. In this study, we found that miR‐3184‐3p was enriched patients and downregulated after resection. We miR‐3184 facilitates progression two ways. On one hand, directly promotes proliferation, migration, invasion while inhibiting apoptosis other glioma‐derived polarizes macrophages to an M2‐like phenotype, which further aggravates progression. Overall, current findings uncovered new mechanism highlighted significant role Furthermore, exosomal be considerable factor with potential applications diagnosis treatment future.

Language: Английский

Citations

27
Methylsulfonylmethane protects against lethal dose MRSA-induced sepsis through promoting M2 macrophage polarization DOI Creative Commons
Wei Ma, Shengxiang Ao, Jianping Zhou

et al.

Molecular Immunology, Journal Year: 2022, Volume and Issue: 146, P. 69 - 77

Published: April 20, 2022

Multi-drug-resistant bacterial infections, which have become a global threat, lack effective treatments. The discoveries of non-antibiotics with different modes antibacterial action, such as methylsulfonylmethane (MSM), are promising new treatment for multi-drug-resistant pathogens.We constructed mouse peritonitis infection model to evaluate the effects MSM against methicillin-resistant Staphylococcus aureus (MRSA) infection. time-kill kinetics MRSA and effect on integrity cell membrane were measured. Viability THP1 cells performed by CCK-8 cytotoxicity assay. Systematic inflammatory factor levels mice detected using ELISA. immune response peritoneal macrophages during MRSA-infection was evaluated RNA sequencing. Gene Ontology function, Kyoto Encyclopedia Genes Genomes pathway enrichment analyses, correlation analyses applied analysis sequencing data. RT-qPCR, western blotting flow cytometry gene protein expression macrophages.In in vitro experiments, did not show significant killing growth directly destroy integrity. also displayed no proliferative capacity cells. However, protected lethal dose decreased systemic inflammation. upregulated metabolic macrophages, especial glycolysis, increased M2 markers (such Arg1), promoted phosphorylation STAT3 (which regulates polarization), M1 macrophages. Additionally, H3K18 lactylation specific target genes, including Arg1. GNE-140, LDHA-specific inhibitor blocked MSM-induced Arg1 this disease model.MSM protects through immunomodulation. promotes lactate-H3K18la control macrophage polarization; it firstly provides therapeutic potential drug-resistant infections sepsis.

Language: Английский

Citations

24
Crucial Roles of RSAD2/viperin in Immunomodulation, Mitochondrial Metabolism and Autoimmune Diseases DOI Creative Commons

Siyan Chen,

Jiani Ye,

Yinfang Lin

et al.

Inflammation, Journal Year: 2024, Volume and Issue: unknown

Published: June 23, 2024

Autoimmune diseases are typically characterized by aberrant activation of immune system that leads to excessive inflammatory reactions and tissue damage. Nevertheless, precise targeted efficient therapies limited. Thus, studies into novel therapeutic targets for the management autoimmune urgently needed. Radical S-adenosyl methionine domain-containing 2 (RSAD2) is an interferon-stimulated gene (ISG) renowned antiviral properties protein it encodes, named viperin. An increasing number have underscored new roles RSAD2/viperin in immunomodulation mitochondrial metabolism. Previous shown there a complex interplay between RSAD2/vipeirn mitochondria binding iron-sulfur (Fe-S) cluster necessary involvement viperin Viperin influences proliferation development cells as well inflammation via different signaling pathways. However, function varies comprehensive overview this emerging theme lacking. This review will describe characteristics RSAD2/viperin, decipher its immunometabolic processes, clarify crosstalk mitochondria. Furthermore, we emphasize crucial RSAD2 potential application value.

Language: Английский

Citations

5
Anti-Inflammatory Activities of Yataprasen Thai Traditional Formulary and Its Active Compounds, Beta-Amyrin and Stigmasterol, in RAW264.7 and THP-1 Cells DOI Creative Commons

Jaenjira Angsusing,

Sudarshan Singh, Weerasak Samee

et al.

Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(8), P. 1018 - 1018

Published: Aug. 1, 2024

Yataprasen (YTPS) remedy formulary, a national Thai traditional medicine comprises 13 herbal plants. It has been extensively prescribed to relieve osteoarthritis and musculoskeletal pain in the healthcare system. The aim of this study was investigate antioxidant anti-inflammatory properties bioactive compounds (β-amyrin stigmasterol) YTPS formulary ethanolic extract, along with its composition. extract contains 70.30 nM β-amyrin 605.76 stigmasterol. exhibited ABTS DPPH free radical scavenging activity, IC

Language: Английский

Citations

5
Tumor-associated macrophages in tumor progression and the role of traditional Chinese medicine in regulating TAMs to enhance antitumor effects DOI Creative Commons
Jiatong Zhang, Jiafeng Gao, Jingwen Cui

et al.

Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13

Published: Oct. 13, 2022

Purpose To emphasize the importance of tumor-associated macrophages (TAMs) in tumor immunity and to describe ways which extracts from Traditional Chinese Medicine (TCM) achieve therapy by modulating macrophages. Significance By summarizing these available data, this review focused on TAMs TCM can build foundation for future research antitumor therapeutics. Methods In review, we summarized key functions cancer development overviewed literature targeting together with other immune cells aiming enhance immunity. Conclusions With an indispensable role immunity, contribute progression, migration, invasion, angiogenesis, lymphangiogenesis, immunosuppressive microenvironment. recent years, has gradually gained attention as a potential adjunctive preclinical clinical trials. is also regulator cytokine secretion cell surface molecule expression balancing microenvironment (TME), especially macrophage activation polarization. Therefore, it believed that could serve modifiers immunomodulatory capability.

Language: Английский

Citations

21
ASIC1a-CMPK2-mediated M1 macrophage polarization exacerbates chondrocyte senescence in osteoarthritis through IL-18 DOI

Lei Dong,

Yingjie Zhao, Cheng Sun

et al.

International Immunopharmacology, Journal Year: 2023, Volume and Issue: 124, P. 110878 - 110878

Published: Sept. 1, 2023

Language: Английский

Citations

12
Macrophage Phenotyping in Atherosclerosis by Proteomics DOI Open Access
Sonia Eligini, Erica Gianazza, Alice Mallia

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(3), P. 2613 - 2613

Published: Jan. 30, 2023

Macrophages are heterogeneous and plastic cells, able to adapt their phenotype functions changes in the microenvironment. They involved several homeostatic processes also many human diseases, including atherosclerosis, where they participate all stages of disease. For these reasons, macrophages have been studied extensively using different approaches, proteomics. Proteomics, indeed, may be a powerful tool better understand behavior careful analysis proteome macrophage phenotypes can help characterize role atherosclerosis provide broad view proteins that might potentially affect course In this review, we discuss proteomic techniques used delineate profile summarize some results elucidate roles develop new strategies counteract progression and/or promote regression.

Language: Английский

Citations

11
IL-4-induced M2 macrophages inhibit fibrosis of endometrial stromal cells DOI
Dan Feng, Yang Li, Hongyun Zheng

et al.

Reproductive Biology, Journal Year: 2024, Volume and Issue: 24(2), P. 100852 - 100852

Published: Feb. 14, 2024

Language: Английский

Citations

4