Immune related gene signature derived from integrate machine learning algorithm predicts outcomes of HNSC patients DOI Creative Commons
Lei Xiao, Yong Liu, Zhe Shen

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 15, 2023

Abstract Background: Due to the highly heterogeneous of tumor, head and neck squamous cancer (HNSC) patients are in variable immunotherapeutic response have different clinical outcomes. We since develop immune related gene signature (IRGS) using a machine learning based integrative procedure for distinguishing microenvironment subtype diverse HNSC then help improve outcomes HNSC. Methods: This study integrate 10 algorithms 111 combination screening out best on 4 multicenter cohorts. Survival analysis, multivariate Cox regression decision curve analysis (DCA) were employed assess performance IRGS. Gene Ontology(GO) Kyoto Encyclopedia Genes Genomesenrichment (KEGG) analyses conducted evaluate potential biological functions mechanisms Results: Through integrated algorithms, we constructed 17-IRG signature, which demonstrated be an excellent prognostic model all cohorts displayed better efficiency when compared with other 68 published signatures. IRGS exhibits strong negative correlation characteristics. The low group demonstrates increased infiltration heightened sensitivity immunotherapy, whereas high higher frequency deletion mutations tumor suppressor genes. Besides, considering insensitive immunotherapy their poor prognosis, scheduled agents strategy selected dasatinib as most target drug patients. Conclusions: was offer more precise selection assessing pre-immune efficacy, will individual

Language: Английский

Rapid and Accurate Diagnosis of Urinary Tract Infections Using Targeted Next-Generation Sequencing: A Multicenter Comparative Study with Metagenomic Sequencing and Traditional Culture Methods DOI Creative Commons
Zhenglin Chang, Jiwang Deng, Jinhu Zhang

et al.

Journal of Infection, Journal Year: 2025, Volume and Issue: 90(4), P. 106459 - 106459

Published: March 7, 2025

BACKGROUNDUrinary tract infections (UTIs) rank among the most prevalent bacterial globally. Traditional urine culture methods have significant limitations in detection time and sensitivity, prompting need to evaluate targeted next-generation sequencing (tNGS) as a potential diagnostic tool.METHODSThe study included discovery cohort of 400 suspected UTI patients (202 analyzed) validation 200 (110 analyzed). The assessed time, concordance rates, ability identify polymicrobial infections, antibiotic resistance genes (ARGs). Both clear turbid samples were evaluated across different clinical settings.RESULTSIn cohort, tNGS demonstrated 96.5% with culture-positive samples, while showing superior specificity culture-negative specimens (53.1% vs 28.1% for mNGS). Detection (12.89h) was notably shorter than mNGS (17.38h) traditional (61.48h). exhibited remarkable capability identifying (55.4% samples), significantly outperforming both (27.7%) methods, which failed detect any co-infections. method showed particular strength detecting fastidious organisms like Ureaplasma parvum fungal species such Candida tropicalis. For prediction, detected more ARGs (52.67% 41.22% mNGS) achieved 100% sensitivity vancomycin methicillin Gram-positive pathogens. confirmed tNGS's robust performance, maintaining high rates (90.00%) (55.00%), demonstrating consistent reliability settingsCONCLUSIONStNGS demonstrates advantages rapid accurate diagnosis, particularly analyzing genes. It shows promise an effective complementary tool diagnostics.

Language: Английский

Citations

2
TRP Ion Channels in Immune Cells and Their Implications for Inflammation DOI Open Access
Qiyue Yan,

Chuanzhou Gao,

Mei Li

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(5), P. 2719 - 2719

Published: Feb. 27, 2024

The transient receptor potential (TRP) ion channels act as cellular sensors and mediate a plethora of physiological processes, including somatosensation, proliferation, apoptosis, metabolism. Under specific conditions, certain TRP are involved in inflammation immune responses. Thus, focusing on the role TRPs system cells may contribute to resolving inflammation. In this review, we discuss distribution five subfamilies mammalian how these function inflammatory mechanisms. This review provides an overview current understanding mediating offer avenues for therapeutic intervention.

Language: Английский

Citations

10
Single-cell and bulk RNA-sequence identified fibroblasts signature and CD8+ T-cell - fibroblast subtype predicting prognosis and immune therapeutic response of bladder cancer, based on machine-learning bioinformatics retrospective study DOI Creative Commons
Jingxian Li, Ning Kong,

Yuanjiong Qi

et al.

International Journal of Surgery, Journal Year: 2024, Volume and Issue: unknown

Published: May 17, 2024

Cancer-associated fibroblasts (CAFs) are found in primary and advanced tumours. They primarily involved tumour progression through complex mechanisms with other types of cells the microenvironment. However, essential fibroblasts-related genes (FRG) bladder cancer still need to be explored, there is a shortage an ideal predictive model or molecular subtype for immune therapeutic assessment cancer, especially muscular-invasive based on FRG.

Language: Английский

Citations

10
Clinical biomarker profiles reveals gender differences and mortality factors in sepsis DOI Creative Commons
Zhenglin Chang,

Jiancai Lu,

Qitai Zhang

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: May 21, 2024

Background Sepsis is a major contributor to global morbidity and mortality, affecting millions each year. Notwithstanding the decline in sepsis incidence mortality over decades, gender disparities outcomes persist, with research suggesting higher rates males. Methods This retrospective study aims delineate gender-specific clinical biomarker profiles impacting progression by examining cases related data from past three years. Propensity score matching was used select age-matched healthy controls for comparison. Results Among 265 patients, significantly proportion were male (60.8%, P<0.001). While did not differ gender, deceased patients older (mean 69 vs 43 years, P=0.003), more likely have hypertension (54% 25%, P=0.019), had SOFA scores ~10 4, P<0.01) compared survivors. Principal Component Analysis (PCA) showed clear separation between controls. 48 serum biomarkers altered sepsis, Triiodothyronine, Apolipoprotein A, Serum cystatin C having highest diagnostic value ROC analysis. Gender-stratified comparisons identified male-specific (e.g. AFP, HDLC) female-specific Rheumatoid factor, Interleukin-6) biomarkers. Deceased differed survivors, 22 differentially expressed markers; Antithrombin, Prealbumin, HDL cholesterol, Urea nitrogen Hydroxybutyrate efficiency mortality. Conclusion These findings enhance our understanding of may guide future therapeutic strategies. Further warranted validate these investigate molecular mechanisms underlying differences outcomes.

Language: Английский

Citations

7
The Application of Single-Cell RNA Sequencing in the Inflammatory Tumor Microenvironment DOI Creative Commons
Jiayi Zhao, Yiwei Shi, Guangwen Cao

et al.

Biomolecules, Journal Year: 2023, Volume and Issue: 13(2), P. 344 - 344

Published: Feb. 10, 2023

The initiation and progression of tumors are complex. cancer evolution-development hypothesis holds that the dysregulation immune balance is caused by synergistic effect genetic factors environmental stimulate maintain non-resolving inflammation. Throughout development process, this inflammation creates a microenvironment for evolution cancer. Research on inflammatory tumor (TME) explains guides anti-cancer immunotherapy. Single-cell RNA sequencing (scRNA-seq) can detect transcription levels cells at single-cell resolution level, reveal heterogeneity evolutionary trajectory infiltrated cells, provide insight into composition function each cell group in TME. This paper summarizes application scRNA-seq

Language: Английский

Citations

16
MiR-125b suppresses bladder Cancer cell growth and triggers apoptosis by regulating IL-6/IL-6R/STAT3 axis in vitro and in vivo DOI
Fang Lin,

Shaorun Hu,

Jinxiang Chen

et al.

Cytokine, Journal Year: 2025, Volume and Issue: 190, P. 156926 - 156926

Published: March 22, 2025

Language: Английский

Citations

0
Organ-specific cancer biomarker identification: a ten-year single-center study in southern China DOI Creative Commons
Zhenglin Chang,

Bingsen Chen,

Suilin Wang

et al.

BMC Cancer, Journal Year: 2025, Volume and Issue: 25(1)

Published: May 1, 2025

Cancer biomarker discovery is essential for early detection and monitoring, yet there a lack of comprehensive studies examining organ-specific biomarkers across various cancer types. In this study, we analyzed clinical data from 59,184 patients diagnosed between 2013 2023, focusing on 11 major systems. We used propensity score matching with 55,010 healthy controls to create balanced comparison groups. Serum profiles were assessed through principal component analysis, differential expression ROC curve analysis. Our findings revealed patterns, such as decreased CA724, ferritin, β2-microglobulin in thoracic cancer, reduced serum phosphorus neurological elevated cystatin C creatinine urinary system cancer. Further analysis 22 types uncovered additional biomarkers, including ALT hepatobiliary altered coagulation factors laryngeal increased monocytes pancreatic complement C3 intestinal These results provide valuable insights into the unique signatures different cancers, contributing potential development more targeted efficient screening methods.

Language: Английский

Citations

0
Spatial immune profiling reveals distinct microenvironments in medullary thyroid carcinoma DOI Creative Commons
María José Castro,

Gustavo Forlin de Siqueira,

J. Mariano

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: May 21, 2025

Introduction Medullary thyroid carcinoma (MTC) is a rare and aggressive cancer with challenging prognosis. While the immune microenvironment plays crucial role in progression, its MTC remains underexplored compared to more common cancers. Methods this study investigates landscape of by systematically evaluating cell infiltration expression markers across various tissue topographies. We utilized advanced immunohistochemical techniques analyze samples from 24 patients, focusing on tumor core, interface healthy tissue, adjacent normal lymph node metastases. Results our findings reveal distinct profile increased CD3+, CD4+, CD8+ CD20+ lymphocytes tissues tumors notable presence granzyme B+ cells interface, particularly patients structural disease. Additionally, we observed significant enrichment mast metastatic tissues. Discussion these results highlight complex spatially dependent MTC, suggesting implications for targeted immunotherapy. This provides novel insights into emphasizes need further research elucidate impact disease progression therapeutic response.

Language: Английский

Citations

0
The genetic associations of COVID-19 on genitourinary symptoms DOI Creative Commons
Zhenglin Chang, Lingyue An, Min Lei

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: June 21, 2023

Recently emerged reports indicated that patients with coronavirus disease 2019 (COVID-19) might experience novo genitourinary symptoms after discharge. Nevertheless, the causal associations and underlying mechanisms remain largely unclear.

Language: Английский

Citations

7
Identification of bladder cancer subtypes and predictive model for prognosis, immune features, and immunotherapy based on neutrophil extracellular trap-related genes DOI Creative Commons
Changhong Guo, Peiying Li,

Xingkui Guo

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Nov. 27, 2023

Language: Английский

Citations

1