Rapid and Accurate Diagnosis of Urinary Tract Infections Using Targeted Next-Generation Sequencing: A Multicenter Comparative Study with Metagenomic Sequencing and Traditional Culture Methods

Journal of Infection, Journal Year: 2025, Volume and Issue: 90(4), P. 106459 - 106459

Published: March 7, 2025

BACKGROUNDUrinary tract infections (UTIs) rank among the most prevalent bacterial globally. Traditional urine culture methods have significant limitations in detection time and sensitivity, prompting need to evaluate targeted next-generation sequencing (tNGS) as a potential diagnostic tool.METHODSThe study included discovery cohort of 400 suspected UTI patients (202 analyzed) validation 200 (110 analyzed). The assessed time, concordance rates, ability identify polymicrobial infections, antibiotic resistance genes (ARGs). Both clear turbid samples were evaluated across different clinical settings.RESULTSIn cohort, tNGS demonstrated 96.5% with culture-positive samples, while showing superior specificity culture-negative specimens (53.1% vs 28.1% for mNGS). Detection (12.89h) was notably shorter than mNGS (17.38h) traditional (61.48h). exhibited remarkable capability identifying (55.4% samples), significantly outperforming both (27.7%) methods, which failed detect any co-infections. method showed particular strength detecting fastidious organisms like Ureaplasma parvum fungal species such Candida tropicalis. For prediction, detected more ARGs (52.67% 41.22% mNGS) achieved 100% sensitivity vancomycin methicillin Gram-positive pathogens. confirmed tNGS's robust performance, maintaining high rates (90.00%) (55.00%), demonstrating consistent reliability settingsCONCLUSIONStNGS demonstrates advantages rapid accurate diagnosis, particularly analyzing genes. It shows promise an effective complementary tool diagnostics.

Language: Английский

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Clinical biomarker profiles reveals gender differences and mortality factors in sepsis

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: May 21, 2024

Background Sepsis is a major contributor to global morbidity and mortality, affecting millions each year. Notwithstanding the decline in sepsis incidence mortality over decades, gender disparities outcomes persist, with research suggesting higher rates males. Methods This retrospective study aims delineate gender-specific clinical biomarker profiles impacting progression by examining cases related data from past three years. Propensity score matching was used select age-matched healthy controls for comparison. Results Among 265 patients, significantly proportion were male (60.8%, P<0.001). While did not differ gender, deceased patients older (mean 69 vs 43 years, P=0.003), more likely have hypertension (54% 25%, P=0.019), had SOFA scores ~10 4, P<0.01) compared survivors. Principal Component Analysis (PCA) showed clear separation between controls. 48 serum biomarkers altered sepsis, Triiodothyronine, Apolipoprotein A, Serum cystatin C having highest diagnostic value ROC analysis. Gender-stratified comparisons identified male-specific (e.g. AFP, HDLC) female-specific Rheumatoid factor, Interleukin-6) biomarkers. Deceased differed survivors, 22 differentially expressed markers; Antithrombin, Prealbumin, HDL cholesterol, Urea nitrogen Hydroxybutyrate efficiency mortality. Conclusion These findings enhance our understanding of may guide future therapeutic strategies. Further warranted validate these investigate molecular mechanisms underlying differences outcomes.

Language: Английский

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Peripheral blood indicators and COVID-19: an observational and bidirectional Mendelian randomization study

BMC Medical Genomics, Journal Year: 2024, Volume and Issue: 17(1)

Published: March 28, 2024

Abstract Blood is critical for health, supporting key functions like immunity and oxygen transport. While studies have found links between common blood clinical indicators COVID-19, they cannot provide causal inference due to residual confounding reverse causality. To identify affecting we analyzed data ( n = 2,293, aged 18–65 years) from Guangzhou Medical University’s first affiliated hospital (2022-present), identifying 34 significant differentiating COVID-19 patients healthy controls. Utilizing bidirectional Mendelian randomization analyses, integrating over 2.46 million participants various large-scale studies, established six with risk, five of which consistent our observational findings. Specifically, elevated Troponin I Platelet Distribution Width levels are linked increased susceptibility, whereas higher Hematocrit, Hemoglobin, Neutrophil counts confer a protective effect. Reverse MR analysis confirmed four biomarkers influenced by aligning three them. Notably, exhibited positive relationship (Tnl) Serum Amyloid Protein A, while negative association was observed Plateletcrit. These findings may help high-risk individuals further direction on the management COVID‐19.

Language: Английский

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Organ-specific cancer biomarker identification: a ten-year single-center study in southern China

BMC Cancer, Journal Year: 2025, Volume and Issue: 25(1)

Published: May 1, 2025

Cancer biomarker discovery is essential for early detection and monitoring, yet there a lack of comprehensive studies examining organ-specific biomarkers across various cancer types. In this study, we analyzed clinical data from 59,184 patients diagnosed between 2013 2023, focusing on 11 major systems. We used propensity score matching with 55,010 healthy controls to create balanced comparison groups. Serum profiles were assessed through principal component analysis, differential expression ROC curve analysis. Our findings revealed patterns, such as decreased CA724, ferritin, β2-microglobulin in thoracic cancer, reduced serum phosphorus neurological elevated cystatin C creatinine urinary system cancer. Further analysis 22 types uncovered additional biomarkers, including ALT hepatobiliary altered coagulation factors laryngeal increased monocytes pancreatic complement C3 intestinal These results provide valuable insights into the unique signatures different cancers, contributing potential development more targeted efficient screening methods.

Language: Английский

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Review of Mendelian randomization studies on common male-specific diseases

Frontiers in Endocrinology, Journal Year: 2025, Volume and Issue: 16

Published: May 16, 2025

Although numerous Mendelian randomization studies on risk factors have been conducted in male medicine, a systematic synthesis of these findings is still lacking. This review searched relevant literature PubMed and the Web Science published before May 2024; systematically summarized progress application infertility, erectile dysfunction, prostate cancer, prostatitis; classified affecting men’s health, such as gut microbiota, modifiable related diseases; presented some problems solutions that were studies. information offers valuable insights into etiology pathogenesis male-specific diseases.

Language: Английский

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Univariable and multivariable mendelian randomization study revealed the modifiable risk factors of urolithiasis

PLoS ONE, Journal Year: 2023, Volume and Issue: 18(8), P. e0290389 - e0290389

Published: Aug. 25, 2023

Urolithiasis is a common urological disease with increasing incidence worldwide, and preventing its risk poses significant challenges. Here, we used Mendelian randomization (MR) framework to genetically assess the causal nature of multifaceted factors on urolithiasis.17 potential associated urolithiasis were collected from recently published observational studies, which can be categorized basically into lifestyle circulating biomarkers. The instrumental variables selected large-scale genome-wide association studies (N ≤ 607,291). Summary-level data obtained UK Biobank (UKB) (3,625 cases 459,308 noncases) FinnGen consortium (5,347 213,445 noncases). univariable multivariable MR analyses applied evaluate causal, independent effect these upon urolithiasis. Effects two consortia combined by meta-analysis methods.Higher predicted sex hormone-binding globulin (SHBG, OR, 0.708; 95% CI, 0.555 0.903), estradiol (OR, 0.179; 0.042 0.751), tea intake 0.550; 0.345 0.878), alcoholic drinks per week 0.992; 0.987 0.997), some physical activity (e.g., swimming, cycling, keeping fit, bowling, 0.054; 0.008 0.363) significantly lower In Multivariate Randomization (MVMR) analyses, associations between estradiol, SHBG, intake, robust even after adjusting for confounding variables. However, previously observed other exercises was no longer factors.The findings highlight roles in development urolithiasis, might provide deeper insight supply preventative strategies.

Language: Английский

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The genetic etiology of body fluids on chronic obstructive airways disease

Respiratory Research, Journal Year: 2024, Volume and Issue: 25(1)

Published: Jan. 19, 2024

Abstract Background Numerous studies have documented significant alterations in the bodily fluids of Chronic Obstructive Pulmonary Disease (COPD) patients. However, existing literature lacks causal inference due to residual confounding and reverse causality. Methods Summary-level data for COPD were obtained from two national biobanks: UK Biobank, comprising 1,605 cases 461,328 controls, FinnGen, with 6,915 186,723 controls. We also validated our findings using clinical 2,690 patients 3,357 healthy controls First Affiliated Hospital Guangzhou Medical University. A total 44 fluid biomarkers selected as candidate risk factors. Mendelian randomization (MR) meta-analyses used evaluate effects these on lung function (FEV1/FVC). Results meta-analyses, by integrating Biobank FinnGen cohort, found that 3 indicators (HDLC, EOS, TP) causally associated COPD, (EOS which is consistent observational findings. Moreover, we noticed EOS TP Conclusions The MR highlight independent roles development (FEV1/FVC), might provide a deeper insight into factors supply potential preventative strategies.

Language: Английский

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Trans-ancestry analysis in over 799,000 individuals yields new insights into the genetic etiology of colorectal cancer

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(7), P. e0301811 - e0301811

Published: July 18, 2024

Background Recent studies have demonstrated the relevance of circulating factors in occurrence and development colorectal cancer (CRC); however, causal relationship remains unclear. Methods Summary-level data for CRC were obtained from UK Biobank (5,657 cases 372,016 controls), FinnGen cohort (3,022 215,770 BioBank Japan Project (BBJ, 7,062 195,745 controls). Thirty-two peripheral markers with consistent definitions collected three biobanks. Mendelian randomization (MR) was used to evaluate effect on CRC. The effects consortiums combined using trans-ancestry meta-analysis methods. Results Our analysis provided compelling evidence association higher genetically predicted eosinophil cell count (EOS, odds ratio [OR], 0.8639; 95% confidence interval [CI] 0.7922–0.9421) red distribution width (RDW, OR, 0.9981; CI, 0.9972–0.9989) levels a decreased risk Additionally, we found suggestive indicating that total cholesterol (TC, 1.0022; 1.0002–1.0042) may increase Conversely, platelet (PLT, 0.9984; 0.9972–0.9996), protein (TP, 0.9445; 0.9037–0.9872), C-reactive (CRP, 0.9991; 0.9983–0.9999) confer protective against Moreover, identified six ancestry-specific factors, necessity considering patients’ ancestry backgrounds before formulating prevention strategies. Conclusions MR findings support independent roles CRC, which might provide deeper insight into early detection supply potential preventative

Language: Английский

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