Fine-tuning spatial-temporal dynamics and surface receptor expression support plasma cell-intrinsic longevity DOI Open Access
Zhixin Jing, Phillip M. Galbo, Luis Ovando

et al.

Published: June 6, 2024

Durable serological memory following vaccination is critically dependent on the production and survival of long-lived plasma cells (LLPCs). Yet, factors that control LLPC specification remain poorly resolved. Using intra-vital two-photon imaging, we find in contrast to most bone marrow (BM), LLPCs are uniquely sessile organized into clusters APRIL, an important factor. deep, bulk RNA sequencing, surface protein flow-based phenotyping, express a unique transcriptome phenotype compared PCs, fine tuning expression key cell molecules, CD93, CD81, CXCR4, CD326, CD44 CD48, for adhesion homing. Conditional deletion Cxcr4 PCs immunization leads rapid mobilization from BM, reduced antigen-specific ultimately accelerated decay antibody titer. In naïve mice, endogenous BCR repertoire exhibits diversity, somatic mutations, increased public clones IgM isotypes, particularly young suggesting non-random. As mice age, BM PC compartment becomes enriched LLPCs, which may outcompete limit entry new niche pool.

Language: Английский

An Update on Anti-COVID-19 Vaccines and the Challenges to Protect Against New SARS-CoV-2 Variants DOI Creative Commons
Fábio Mambelli, Ana Carolina V. S. C. de Araujo, Jéssica Pires Farias

et al.

Pathogens, Journal Year: 2025, Volume and Issue: 14(1), P. 23 - 23

Published: Jan. 1, 2025

The COVID-19 pandemic has posed a significant threat to global health systems, with extensive impacts across many sectors of society. been responsible for millions deaths worldwide since its first identification in late 2019. Several actions have taken prevent the disease, including unprecedented fast development and vaccination campaigns, which were pivotal reducing symptoms deaths. Given impact pandemic, continuous changes virus, present vaccine technologies, this review analyzes how, so far, we met challenge by emergence new variants discusses how next-generation pan-coronavirus vaccines, enhanced longevity breadth immune responses, may be tackled alternative administration routes antigen delivery platforms. By addressing these critical aspects, aims contribute ongoing efforts achieve long-term control COVID-19, stimulating discussion work on vaccines capable facing future waves infection.

Language: Английский

Citations

2
Measurement of IFN-γ and IL-2 for the assessment of the cellular immunity against SARS-CoV-2 DOI Creative Commons
Guillem Safont, Raquel Villar-Hernández,

Daria Smalchuk

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Jan. 11, 2024

The study of specific T-cell responses against SARS-CoV-2 is important for understanding long-term immunity and infection management. aim this was to assess the dual IFN-γ IL-2 detection, using a fluorescence ELISPOT, in patients undergoing acute disease, during convalescence, after vaccination. We also evaluated humoral response compared with T-cells correlating both types responses, increase number detection. Blood samples were drawn from COVID-19 convalescent individuals classified according disease severity; unvaccinated vaccinated uninfected individuals. IgGs Spike nucleocapsid, IgMs neutralizing antibodies analyzed. Our results show that combination increases detection (p = 0.023). In addition, can be useful biomarker monitoring severe patients, as our indicate those poor outcome have lower levels cytokine. some cases, lack cellular compensated by antibodies, confirming role immune infection, their detections. summary, IFN-γ/IL-2 promising characterizing assessing immunization status, helping patient

Language: Английский

Citations

12
Age-related changes in the immune system and challenges for the development of age-specific vaccines DOI Creative Commons
Mark Doherty, Birgit Weinberger, Arnaud M. Didierlaurent

et al.

Annals of Medicine, Journal Year: 2025, Volume and Issue: 57(1)

Published: March 20, 2025

Background A better understanding of how the immune system evolves with age and vaccines work in older people has led to increasing focus on development aimed specifically at groups. We discuss strategies used improve vaccine immunogenicity for adults, focusing licensed adjuvants.

Language: Английский

Citations

1
Factors Associated with Anti-SARS-CoV-2 Vaccine Acceptance among Pregnant Women: Data from Outpatient Women Experiencing High-Risk Pregnancy DOI Creative Commons
Marianna Maranto, Giuseppe Gullo, Alessandra Nejar Bruno

et al.

Vaccines, Journal Year: 2023, Volume and Issue: 11(2), P. 454 - 454

Published: Feb. 16, 2023

Pregnant women are at higher risk of severe Coronavirus disease 2019 (COVID-19) complications than non-pregnant women. The initial exclusion pregnant from anti-SARS-CoV-2 vaccines clinical trials has caused a lack conclusive data about safety and efficacy for this vulnerable population. This cross-sectional study aims to define the factors related vaccination adherence in sample experiencing high-risk pregnancies. recruited completed questionnaire based on Health Belief Model. Data were analyzed evaluate associations between socio-demographic variables acceptance through descriptive, univariate multivariate logistic analyses. Among 233 enrolled, 65.2% (n = 152) declared that they would accept vaccine. Multivariate analysis showed was associated with high educational level (aOR 4.52, p 0.001), low perception barriers 1.58, 0.005) gynecologist’s advice 3.18, 0.01). About third still hesitant vaccine, probably because conflicting information received media, friends, acquaintances health institutions. Determining linked vaccine hesitancy among is useful creating strategies increase uptake.

Language: Английский

Citations

17
Correlation between CD4 T-Cell Counts and Seroconversion among COVID-19 Vaccinated Patients with HIV: A Meta-Analysis DOI Creative Commons
Qian Zhou,

Yihuang Liu,

Furong Zeng

et al.

Vaccines, Journal Year: 2023, Volume and Issue: 11(4), P. 789 - 789

Published: April 4, 2023

To evaluate the potential factors for predicting seroconversion due to coronavirus disease 2019 (COVID-19) vaccine in people living with HIV (PLWH).We searched PubMed, Embase and Cochrane databases eligible studies published from inception 13th September 2022 on predictors of serologic response COVID-19 among PLWH. This meta-analysis was registered PROSPERO (CRD42022359603).A total 23 comprising 4428 PLWH were included meta-analysis. Pooled data demonstrated that about 4.6 times patients high CD4 T-cell counts (odds ratio (OR) = 4.64, 95% CI 2.63 8.19) compared those low counts. Seroconversion 17.5 receiving mRNA vaccines (OR 17.48, 6.16 49.55) other types vaccines. There no differences different ages, gender, viral load, comorbidities, days after complete vaccination, type. Subgroup analyses further validated our findings predictive value range, 2.30 9.59).The associated vaccinated Precautions should be emphasized these counts, even a course vaccination.

Language: Английский

Citations

14
Prolonged SARS-CoV-2 T Cell Responses in a Vaccinated COVID-19-Naive Population DOI Creative Commons
Vassiliki Pitiriga, Myrto Papamentzelopoulou,

Kanella E. Konstantinakou

et al.

Vaccines, Journal Year: 2024, Volume and Issue: 12(3), P. 270 - 270

Published: March 4, 2024

Exploring T cell response duration is pivotal for understanding immune protection evolution in natural SARS-CoV-2 infections. The objective of the present study was to analyze over time individuals who were both vaccinated and COVID-19-naive had undetectable levels IgG antibodies at testing.

Language: Английский

Citations

5
Homologous or heterologous administration of mRNA or adenovirus-vectored vaccines show comparable immunogenicity and effectiveness against the SARS-CoV-2 Omicron variant DOI Creative Commons
G Pastore, Jacopo Polvere, Fabio Fiorino

et al.

Expert Review of Vaccines, Journal Year: 2024, Volume and Issue: 23(1), P. 432 - 444

Published: March 22, 2024

Heterologous prime-boost schedules have been employed in SARS-CoV-2 vaccination, yet additional data on immunogenicity and effectiveness are still needed.

Language: Английский

Citations

5
Longitudinal Follow‐Up of the Specific Antibody Response to SARS‐CoV‐2 Vaccination in Colombia DOI Creative Commons
Myriam Arévalo‐Herrera, Bladimiro Rincón‐Orozco,

John Mario González‐Escobar

et al.

Journal of Medical Virology, Journal Year: 2025, Volume and Issue: 97(1)

Published: Jan. 1, 2025

ABSTRACT A total of 5011 adult volunteers attending vaccination centers in different regions Colombia were enrolled a 1‐year prospective observational cohort study to evaluate the immunogenicity and effectiveness SARS‐CoV‐2‐based vaccines as part National Vaccine Program established contain COVID‐19 pandemic. Following informed consent, 5,011 participants underwent sociodemographic survey PCR testing assess SARS‐CoV‐2 infection. Blood samples collected, serum fractions obtained from participant subsample ( n = 3441) at six‐time points virus‐specific IgG responses Spike protein, its Receptor Binding Domain, Nucleoprotein by ELISA. Additionally, antibody‐neutralizing activity was evaluated using cPass neutralization kit. Most (95.8%; 4802) received between one Ad26. COV2.S (Janssen vaccine) four vaccine doses BNT162b2 (Pfizer/BioNTech), AZD1222 (AstraZeneca), mRNA‐1273 (Moderna), CoronaVac (Sinovac), with some receiving combinations; small group, 4.2% 209), remained unvaccinated. Throughout study, only 8.76% 439) tested positive for PCR. Notably, all seroconverted antibodies, high seropositivity rates S (99.8%; 4795), RBD (99.7%; 1691), N (92.7%; 3072) proteins. Moreover, significant (92%–97%) neutralizing observed circulating variants. This highlights importance assessing duration response elicited infection, antibody potential surrogate marker protection. These findings provide important insight further strengthening strategies control COVID‐19.

Language: Английский

Citations

0
JN.1 variants circulating in Italy from October 2023 to April 2024: genetic diversity and immune recognition DOI Creative Commons
Emanuela Giombini, Ilaria Schiavoni, Luigina Ambrosio

et al.

BMC Infectious Diseases, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 28, 2025

The continuous emergence of SARS-CoV-2 variants and subvariants poses significant public health challenges. latest designated subvariant JN.1, with all its descendants, shows more than 30 mutations in the spike gene. JN.1 has raised concerns due to genomic diversity potential enhance transmissibility immune evasion. This study aims analyse molecular characteristics JN.1-related lineages (JN.1*) identified Italy from October 2023 April 2024 evaluate neutralization activity against a subsample sera individuals vaccinated XBB.1.5 mRNA. gene 794 JN.1* strain was evaluated phylogenetic analysis conducted compare distance XBB.1.5. Moreover, serum assays were performed on 19 healthcare workers (HCWs) monovalent mRNA booster assess neutralizing capacity JN.1. Sequence displayed high variability between investigation confirmed substantial differentiation regions 29 shared mutations, which 17 located within RBD region. Pre-booster observed 42% HCWs sera, increasing significantly post-booster, showing three months after vaccination. A correlation found anti-trimeric Spike IgG levels titers highlights Italy. Results vaccine suggested enhanced

Language: Английский

Citations

0
The evolving role of B cells in malignancies DOI
Samik Bindu,

Roshni Bibi,

Roshini Pradeep

et al.

Human Immunology, Journal Year: 2025, Volume and Issue: 86(3), P. 111301 - 111301

Published: March 25, 2025

Language: Английский

Citations

0