Platelet-neutrophil interaction in COVID-19 and vaccine-induced thrombotic thrombocytopenia

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: May 19, 2023

Coronavirus disease 2019 (COVID-19) is known to commonly induce a thrombotic diathesis, particularly in severely affected individuals. So far, this COVID-19-associated coagulopathy (CAC) has been partially explained by hyperactivated platelets as well the prothrombotic effects of neutrophil extracellular traps (NETs) released from neutrophils. However, precise insight into bidirectional relationship between and neutrophils pathophysiology CAC still lags behind. Vaccine-induced thrombocytopenia (VITT) rare autoimmune disorder caused auto-antibody formation response immunization with adenoviral vector vaccines. VITT associated life-threatening thromboembolic events thus, high fatality rates. Our concept thrombophilia observed relatively new, hence better understanding could help management such patients potential also prevent VITT. In review we aim summarize current knowledge on platelet-neutrophil interplay COVID-19

Language: Английский

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Association between urinary metals and prostate-specific antigen in aging population with depression: a cross-sectional study

Frontiers in Public Health, Journal Year: 2024, Volume and Issue: 12

Published: May 23, 2024

Objective This study aims to investigate the impact of depression and urinary metals on Prostate-Specific Antigen (PSA). Methods Analysis was conducted 1901 samples collected from National Health Nutrition Examination Survey (NHANES) database between 2001 2010. Analytical methods included stepwise multiple linear regression (MLR) analysis overall population’s PSA relationship, relationship in older adults BMI subgroups, depressed population, restricted cubic spline (RCS) analysis. A significance level p < 0.05 considered statistically significant. Results In regression, beryllium (Be) showed a dose–response association with (third quartile: β = 0.05, 95%CI (0.02, 0.09); fourth 0.07, 0.12), trend 0.048). Subgroup indicated that individuals aged >60, Be at Q4 [β 0.09, (0.05, 0.21)] exhibited correlation PSA. population 25 ≤ 30, might more significantly elevate PSA, having pronounced levels 0.03, 1.27)]. cadmium (Cd) significant positive Cd maximum 0.3, (0.09, 0.49)]. Conclusion Individuals exposed (Be), especially overweight, should monitor their levels. patients, may further levels, necessitating increased monitoring among males.

Language: Английский

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Mitochondria in COVID-19: from cellular and molecular perspective

Frontiers in Physiology, Journal Year: 2024, Volume and Issue: 15

Published: June 21, 2024

The rapid development of the COVID-19 pandemic resulted in a closer analysis cell functioning during β-coronavirus infection. This review will describe evidence for as syndrome with strong, albeit still underestimated, mitochondrial component. Due to sensitivity host mitochondria coronavirus infection, SARS-CoV-2 affects signaling, modulates immune response, modifies cellular energy metabolism, induces apoptosis and ageing, worsening symptoms which can sometimes be fatal. Various aberrations across human systems tissues their relationships were reported. In this review, particular attention is given characterization multiple alterations gene expression pattern metabolism COVID-19; complexity interactions between proteins presented. participation mitogenome fragments signaling occurrence subgenomic RNA within membranous compartments, including widely discussed. As severely quality system mitochondria, background dynamics additionally characterized. Finally, perspectives on mitigation by affecting biogenesis numerous compounds therapeutic treatments are briefly outlined.

Language: Английский

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The role of neutrophilia in hyperlactatemia, blood acidosis, impaired oxygen transport, and mortality outcome in critically ill COVID-19 patients

Frontiers in Molecular Biosciences, Journal Year: 2025, Volume and Issue: 11

Published: Jan. 6, 2025

COVID-19 severity and high in-hospital mortality are often associated with severe hypoxemia, hyperlactatemia, acidosis, yet the key players driving this association remain unclear. It is generally assumed that organ damage causes toxic but since neutrophil numbers in can exceed 80% of total circulating leukocytes, we asked if metabolic acidosis mediated by glycolytic neutrophils lung impaired oxygen delivery critically ill patients. Based on prospective outcome, patients were divided into ICU- survivors ICU-non-survivors. Samples analyzed to explore correlations exist between counts, damage, glycolysis, blood lactate, pH, hemoglobin saturation, outcome. We also interrogated isolated neutrophils, platelets, PBMCs for activities. Arterial gas analyses showed remarkable hypoxemia non-survivors no consistent differences PCO2 or [HCO3 -]. The dissociation curve revealed a right-shift, lower blood-pH elevated lactate non-survivors. Metabolic analysis different cells increased activity only when considering number neutrophils. This indicates role neutrophilia hyperlactatemia subsequently contributing outcomes SARS-CoV-2 infection.

Language: Английский

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Development of a risk prediction model for secondary infection in severe/critical COVID-19 patients

BMC Infectious Diseases, Journal Year: 2025, Volume and Issue: 25(1)

Published: May 21, 2025

This study aimed to develop a predictive model for secondary infections in patients with severe or critical COVID-19 by analyzing clinical characteristics and laboratory indicators. A total of 307 admitted Peking University Third Hospital from December 2022 February 2023 were retrospectively analyzed, including 156 infection 151 without infection. The Boruta algorithm identified significant variables, eight machine learning models evaluated based on area under the curve (AUC) performance. optimal selected was further assessed, interpretability provided using SHapley Additive exPlanations (SHAP). Nine factors identified: Mechanical Ventilation, Procalcitonin (PCT), Interleukin-8 (IL-8), Interleukin-6 (IL-6), Blood Urea Nitrogen, Glucose, Creatine Kinase, Lactate Dehydrogenase, Mean Platelet Volume (MPV). random forest demonstrated best performance, evaluation showing an average AUC 0.981 (CI 0.965-0.998) training set 0.836 0.761-0.912) test set. SHAP analysis MPV, PCT, IL-8 as strongest predictors infections. We developed effective risk readily available parameters, enabling early intervention. approach demonstrates potential improving patient management. does not involve trial interventions. Therefore, registration applicable.

Language: Английский

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Comparison of Different Vascular Biomarkers for Predicting In-Hospital Mortality in Severe SARS-CoV-2 Infection

Microorganisms, Journal Year: 2024, Volume and Issue: 12(1), P. 229 - 229

Published: Jan. 22, 2024

Severe SARS-CoV-2 elicits a hyper-inflammatory response that results in intravascular inflammation with endothelial injury, which contributes to increased mortality COVID-19. To predict the outcome of severe infection, we analyzed baseline level different biomarkers vascular disorders COVID-19 subjects upon intensive care unit (ICU) admission and prior any vaccination. A total 70 patients (37 survivors 33 non-survivors) were included 16 age- sex-matched controls. Vascular dysfunction was monitored via soluble VCAM-1, E-selectin, ACE2 Lp-PLA2, while abnormal platelet activation evaluated by P-selectin CD40L parallel. These correlated routine laboratory parameters disease outcomes. Among these parameters, VCAM-1 showed significantly higher serum levels early death vs. convalescent subjects. Horowitz index (r = 0.3115) IL-6 0.4599), related E-selectin 0.4143) 0.2948). Lp-PLA2 altered none subcohorts no relationship other parameters. Finally, pre-treatment (≥1420 ng/mL) activity (≥45.2 μU/mL) predicted larger risk for (Log-Rank

Language: Английский

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An early warning indicator of mortality risk in patients with COVID-19: the neutrophil extracellular traps/neutrophilic segmented granulocyte ratio

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Jan. 29, 2024

Background Neutrophil extracellular traps (NETs) play a key role in thrombus formation patients with coronavirus disease 2019 (COVID-19). However, the existing detection and observation methods for NETs are limited their ability to provide quantitative, convenient, accurate descriptions of situ NETs. Therefore, establishing quantitative description relationship between thrombosis remains challenge. Objective We employed morphological observations blood cells statistical analyses investigate correlation NETs/neutrophilic segmented granulocyte ratio mortality risk COVID-19. Methods Peripheral samples were collected from 117 hospitalized COVID-19 November 2022 February 2023, various cell parameters measured. Two types smudge observed counted: lymphatic neutral cells. Statistical data analysis was used establish assessment indicators. Results Morphological neutrophilic revealed swelling, eruption, neutrophil nuclei. Subsequently, (NNSR) calculated. A high concentration poses fatal patients. indicated that NNSR more suitable evaluating death compared elevated fibrinogen (FIB) D-dimer (DD) levels. Conclusion Observing morphology is an effective method NETs, important markers revealing

Language: Английский

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Platelet dysfunction caused by differentially expressed genes as key pathogenic mechanisms in COVID-19

Minerva Cardiology and Angiology, Journal Year: 2024, Volume and Issue: 72(5)

Published: May 28, 2024

At the end of 2019, novel coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome 2 (SARS-CoV-2) became prevalent worldwide, which brought a heavy medical burden and tremendous economic losses to world population. In addition common clinical symptoms such as fever, cough headache, patients with COVID-19 often have hematological diseases, especially platelet dysfunction. Platelet dysfunction usually leads multiple organ dysfunction, is closely related patient severity or mortality. addition, studies confirmed significant changes in gene expression profile circulating platelets under SARS-CoV-2 infection, will further lead function. same time, shown that may absorb SARS-COV-2 mRNA independently ACE2, emphasizes importance stability function defense against infection. This study reviewed relationship between damage circulatory system, analyzed significantly differentially expressed after infection on basis previous studies. The top eight hub genes were identified NLRP3, MT-CO1, CD86, ICAM1, MT-CYB, CASP8, CXCL8 CXCR4. Subsequently, effects transcript abnormalities explored 8 genes. Finally, treatment measures complications discussed detail, so provide reference for prevention, diagnosis COVID-19.

Language: Английский

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Platelet Ido1 expression is induced during Plasmodium yoelii infection, altering plasma tryptophan metabolites

Blood Advances, Journal Year: 2024, Volume and Issue: 8(22), P. 5814 - 5825

Published: Aug. 12, 2024

Language: Английский

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СТРЕС І ФОРМУВАННЯ КОМОРБІДНОГО ПЕРЕБІГУ ЛОНГ-КОВІДУ ТА УРГЕНТНОЇ ХІРУРГІЧНОЇ ПАТОЛОГІЇ

Immunology and Allergy Science and Practice, Journal Year: 2024, Volume and Issue: 1, P. 10 - 15

Published: April 22, 2024

Вступ. Збільшення кількості пацієнтів з невідкладними хірургічними станами черевної порожнини може бути пов’язано періодичним поширенням вірусної інфекції SARS-CoV-2 і формуванням постковідного синдрому. Різні штами вірусу відрізняються специфічністю до рецепторів клітин- мішеней, а їх дія викликає різноманітність симптомів. Важливим є визначення чинників, які сприяють високому розвитку синдрому, маніфестації хронічних захворювань виникнення ургентної хірургічної абдомінальної патології. Мета роботи – виявити характер та ступінь порушень показників вродженого адаптивного імунітету у коморбідними ур-гентною хірургічною абдомінальною патологією на тлі COVID-19 віддалений період при синдрому (ПКС). Матеріали методи. Обстежено 115 невідкладною патологією, яких відмічали симптоми ПКС. Використовували методи: цитометрії (визначення лейкоцитів, лімфоцитів, нейтрофілів, тромбоцитів); флуоресцентної мікроскопії (показники фагоцитуючих кількість позаклітинних нейтрофільних пасток); УФ-спектроскопія (спектри поглинання сироватки крові); спектрофотометрія (вміст цитотоксичних фракцій DAMP); імуноферментний аналіз білків МСР-1 S100B). Результати обговорення. У обстежених ургентною одужали, виявили високу концентрацію DAMP; померлих було більш виражене збільшення олігонуклеотидної фракції, що супроводжувалось максимальною зміною імунних показників: вмісту специфічних S100В МСР-1, пригнічення нейтрофілів (зниження активних фагоцитів індексу перетравлювання). А пасток була компенсаторно підвищеною пацієнтів, одужали. Висновок. Розвиток симптомів, тяжкість перебігу патології, формування ПКС ризик летальності взаємопов’язані природою, величиною співвідношенням DАМР відповідь імунітету.

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