Matrix metalloproteinase-12 by M2 macrophages induced epithelial to mesenchymal transition in chronic rhinosinusitis with nasal polyps DOI Creative Commons
Joo‐Hoo Park, Jae‐Min Shin,

Hyun-Woo Yang

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(12), P. e0313097 - e0313097

Published: Dec. 31, 2024

Th2 inflammation and epithelial-mesenchymal transition (EMT) play crucial roles in the pathophysiology of chronic rhinosinusitis with nasal polyps (CRSwNP). This study aimed to investigate hypothesis that MMP-12, produced by M2 macrophages, induces EMT epithelial cells, thereby contributing airway remodeling CRSwNP. The expression levels MMP-12 were measured RT-PCR CRS mucosa THP-1 cells. mRNA protein E-cadherin, vimentin, α-SMA, fibronectin determined using RT-PCR, western blotting, immunofluorescence staining primary cells air-liquid interface culture. was significantly increased CRSwNP M2-like In co-culture E-cadherin inhibited, fibronectin, α-SMA increased. decreased but induced MMP408, an inhibitor, inhibited EMT-related factors. These findings suggest macrophages may contribute pathogenesis

Language: Английский

Macrophage plasticity: signaling pathways, tissue repair, and regeneration DOI Creative Commons
Lingfeng Yan,

Jue Wang,

Xin Cai

et al.

MedComm, Journal Year: 2024, Volume and Issue: 5(8)

Published: Aug. 1, 2024

Abstract Macrophages are versatile immune cells with remarkable plasticity, enabling them to adapt diverse tissue microenvironments and perform various functions. Traditionally categorized into classically activated (M1) alternatively (M2) phenotypes, recent advances have revealed a spectrum of macrophage activation states that extend beyond this dichotomy. The complex interplay signaling pathways, transcriptional regulators, epigenetic modifications orchestrates polarization, allowing respond stimuli dynamically. Here, we provide comprehensive overview the cascades governing focusing on roles Toll‐like receptors, signal transducer activator transcription proteins, nuclear microRNAs. We also discuss emerging concepts metabolic reprogramming trained immunity, contributing their functional adaptability. Macrophage plasticity plays pivotal role in repair regeneration, macrophages coordinating inflammation, angiogenesis, matrix remodeling restore homeostasis. By harnessing potential novel therapeutic strategies targeting polarization could be developed for diseases, including chronic wounds, fibrotic disorders, inflammatory conditions. Ultimately, deeper understanding molecular mechanisms underpinning will pave way innovative regenerative medicine engineering approaches.

Language: Английский

Citations

27
ALOX15+ M2 macrophages contribute to epithelial remodeling in eosinophilic chronic rhinosinusitis with nasal polyps DOI
Chang Liu, Kanghua Wang, Wenqin Liu

et al.

Journal of Allergy and Clinical Immunology, Journal Year: 2024, Volume and Issue: 154(3), P. 592 - 608

Published: May 3, 2024

Language: Английский

Citations

13
Role of Nasal Fibroblasts in Airway Remodeling of Chronic Rhinosinusitis: The Modulating Functions Reexamined DOI Open Access
Jae‐Min Shin,

Hyun Woo Yang,

Jae Hyung Park

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(4), P. 4017 - 4017

Published: Feb. 16, 2023

Chronic rhinosinusitis (CRS) is a multifactorial inflammatory disease of the nose and sinuses that affects more than 10% adult population worldwide. Currently, CRS classified into endotypes according to response (Th1, Th2, Th17) or distribution immune cells in mucosa (eosinophilic non-eosinophilic). induces mucosal tissue remodeling. Extracellular matrix (ECM) accumulation, fibrin deposition, edema, cell infiltration, angiogenesis are observed stromal region. Conversely, epithelial-to-mesenchymal transition (EMT), goblet hyperplasia, increased epithelial permeability, metaplasia found epithelium. Fibroblasts synthesize collagen ECM, which create structural skeleton play an important role wound-healing process. This review discusses recent knowledge regarding modulation remodeling by nasal fibroblasts CRS.

Language: Английский

Citations

14
Integrative analysis of metabolomics and transcriptomics to uncover biomarkers in sepsis DOI Creative Commons
Wenhao Chen, Wentao Guo, Yang Li

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: April 27, 2024

Abstract To utilize metabolomics in conjunction with RNA sequencing to identify biomarkers the blood of sepsis patients and discover novel targets for diagnosing treating sepsis. In January 2019 December 2020, samples were collected from a cohort 16 diagnosed 11 systemic inflammatory response syndrome (SIRS). Non-targeted analysis was conducted using liquid chromatography coupled mass spectrometry (LC–MS/MS technology), while gene performed sequencing. Afterward, metabolite data underwent quality control difference analysis, fold change (FC) greater than or equal 2 false discovery rate (FDR) less 0.05.Co-analysis then differential factors consistent expression trends based on metabolic pathway context; KEGG enrichment crossover factors, Meta-analysis at transcriptome level public dataset. end, total five single nucleated cells peripheral (two normal controls, one syndrome, two sepsis) examined determine cellular location essential genes 10× cell (scRNA-seq). A 485 1083 metabolites found be differentially expressed group compared SIRS group. Among these, 40 both metabolome transcriptome. Functional revealed that these primarily involved biological processes related response, immune regulation, amino acid metabolism. Furthermore, meta-analysis identified four genes, namely ITGAM, CD44, C3AR1, IL2RG, which highly ( P < 0.05). Additionally, scRNA-seq core ITGAM C3AR1 predominantly localized within macrophage lineage. The primary exhibit predominant macrophages, play significant role responses. Moreover, show elevated levels plasma individuals sepsis, indicating their potential as valuable subjects further research

Language: Английский

Citations

5
Molecular dynamics of chemotactic signalling orchestrates dental pulp stem cell fibrosis during aging DOI Creative Commons
Tianmeng Sun, Qing Zhong, Xiaoyi Yu

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 12

Published: Jan. 10, 2025

Aging often triggers dental pulp fibrosis, resulting in clinical repercussions such as increased susceptibility to infections, compromised tooth vitality, and reduced responsiveness interventions. Despite its prevalence, the precise molecular mechanisms underlying this condition remains unclear. Leveraging single-cell transcriptome analysis from both our own publicly available datasets, we identified Ccrl2+ macrophages particularly vulnerable during early stages of aging. Notably, progenitors with high expression RARRES2, a unique ligand for CCRL2, facilitate selective recruitment specific macrophage population stem cell niches. This process culminates formation ligand-receptor complex that engages CMKLR1, receptor broadly expressed across populations. interaction drives activation expansion through RARRES2/CCRL2/CMKLR1 axis. Through rigorous experimental validation, demonstrated within niches lead secretion proinflammatory factors, promoting fibrosis Our findings uncover intricate dynamics aging, emphasizing immune microenvironment interactions. study provides novel perspective on potential therapeutic strategies age-related diseases by targeting modulating microenvironment.

Language: Английский

Citations

0
Macrophage polarization-mediated PKM2/mTORC1/YME1L signaling pathway activation in fibrosis associated with Cardiorenal syndrome DOI
Xuefeng Zhang, Wenyu Shao,

Yun Gao

et al.

Cellular Signalling, Journal Year: 2025, Volume and Issue: unknown, P. 111664 - 111664

Published: Feb. 1, 2025

Language: Английский

Citations

0
Multi-omics integration and machine learning identify and validate neutrophil extracellular trap-associated gene signatures in chronic rhinosinusitis with nasal polyps DOI
Fu Shu, Yaping Wang, Linglong Li

et al.

Clinical Immunology, Journal Year: 2025, Volume and Issue: 275, P. 110473 - 110473

Published: March 13, 2025

Language: Английский

Citations

0
The characterization and validation of regulated cell death-related genes in chronic rhinosinusitis with nasal polyps DOI
Hongxia Li, Fei Jing, Wei Xu

et al.

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 154, P. 114509 - 114509

Published: March 31, 2025

Language: Английский

Citations

0
Overexpression of AXL on macrophages associates with disease severity and recurrence in chronic rhinosinusitis with nasal polyps DOI

Tiansheng Wang,

Yu Chen, Ru Gao

et al.

International Immunopharmacology, Journal Year: 2023, Volume and Issue: 121, P. 110449 - 110449

Published: June 10, 2023

Language: Английский

Citations

10
Unraveling the Role of Epithelial Cells in the Development of Chronic Rhinosinusitis DOI Open Access
Jong‐Gyun Ha, Hyung‐Ju Cho

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(18), P. 14229 - 14229

Published: Sept. 18, 2023

The pathophysiology of CRS is multifactorial and complex yet needs to be completed. Recent evidence emphasizes the crucial part played by epithelial cells in development CRS. act as physical barriers play roles host defense, including initiating shaping innate adaptive immune responses. This review aims present a comprehensive understanding significance nasal New research suggests that dysfunction plays role developing through multiple mechanisms. refers issues with weakened barrier function, disrupted mucociliary clearance, irregular When compromised, it can lead passage pathogens allergens, triggering inflammation body. Furthermore, impaired clearance accumulate secretions inflammatory mediators, promoting chronic inflammation. Epithelial release cytokines chemokines, which attract activate cells. result an imbalanced response continues cause interaction between various leads production either increase or decrease By comprehending CRS, we enhance our disease's pathogenesis explore new therapeutics.

Language: Английский

Citations

9