MicroRNAs in Juvenile Idiopathic Arthritis: State of the Art and Future Perspectives DOI Creative Commons
Simone Pelassa, Federica Raggi, Chiara Rossi

et al.

Biology, Journal Year: 2023, Volume and Issue: 12(7), P. 991 - 991

Published: July 12, 2023

Juvenile Idiopathic Arthritis (JIA) represents the most common chronic pediatric arthritis in Western countries and a leading cause of disability children. Despite recent clinical achievements, patient management is still hindered by lack diagnostic/prognostic biomarkers targeted treatment protocols. MicroRNAs (miRNAs) are short non-coding RNAs playing key role gene regulation, their involvement many pathologies has been widely reported literature. In decades, miRNA’s contribution to regulation immune system pathogenesis autoimmune diseases demonstrated. Furthermore, miRNAs isolated from patients’ biological samples currently under investigation for potential as novel biomarkers. This review aims provide an overview state art on miRNA JIA. The literature addressing expression different types JIA patients was reviewed, focusing particular application responses affected joints will also be discussed along with utility markers therapeutic approaches. information value investigators field rheumatology, encouraging further research increase our knowledge miRNAs’ future applications

Language: Английский

The Circulating Biomarkers League: Combining miRNAs with Cell-Free DNAs and Proteins DOI Open Access

Kyriacos Felekkis,

Christos Papaneophytou

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(6), P. 3403 - 3403

Published: March 17, 2024

The potential of liquid biopsy for the prognosis and diagnosis diseases is unquestionable. Within evolving landscape disease diagnostics personalized medicine, circulating microRNAs (c-miRNAs) stand out among biomarkers found in blood circulation other biological fluids due to their stability, specificity, non-invasive detection biofluids. However, complexity human limitations inherent single-marker highlight need a more integrative approach. It has been recently suggested that multi-analyte approach offers advantages over single-analyte diseases. In this review, we explore combining three well-studied classes biofluids—miRNAs, DNAs, proteins—to enhance accuracy efficacy monitoring. Initially, provide an overview each biomarker class discuss main disadvantages highlighting superiority c-miRNAs biomarkers. Additionally, challenges future directions integrating these into clinical practice, emphasizing standardized protocols further validation studies. This integrated revolutionize precision medicine by offering insights mechanisms, facilitating early detection, guiding therapeutic strategies. collaborative power with represents promising frontier comprehensive understanding management complex Nevertheless, several must be addressed before can translated practice.

Language: Английский

Citations

19
Exposome: Epigenetics and autoimmune diseases DOI Creative Commons
Maria Giovanna Danieli, Marco Casciaro, Alberto Paladini

et al.

Autoimmunity Reviews, Journal Year: 2024, Volume and Issue: 23(6), P. 103584 - 103584

Published: June 1, 2024

Systemic autoimmune diseases are complex conditions characterized by an immune system dysregulation and aberrant activation against self-antigens, leading to tissue organ damage. Even though genetic predisposition plays a role, it cannot fully explain the onset of these diseases, highlighting significant impact non-heritable influences such as environment, hormones infections. The exposome represents all those factors, ranging from chemical pollutants dietary components psychological stressors infectious agents. Epigenetics, which studies changes in gene expression without altering DNA sequence, is crucial link between development diseases. Key epigenetic mechanisms include methylation, histone modifications, non-coding RNAs. These modifications could provide potential piece puzzle understanding systemic their connection with exposome. In this work we have collected most important recent evidence linked (systemic lupus erythematosus, idiopathic inflammatory myopathies, ANCA-associated vasculitis, rheumatoid arthritis), emphasizing roles may play disease pathogenesis, diagnostic biomarkers prospective targeted therapies.

Language: Английский

Citations

10
Exosomal miRNAs-mediated macrophage polarization and its potential clinical application DOI

Ming yun Yu,

Hui jie Jia,

Jing Zhang

et al.

International Immunopharmacology, Journal Year: 2023, Volume and Issue: 117, P. 109905 - 109905

Published: Feb. 27, 2023

Language: Английский

Citations

15
Effect of M0 macrophage-derived exosome miR-181d-5p targeting BCL-2 to regulate NLRP3/caspase-1/GSDMD pathway on human renal mesangial cells pyroptosis DOI

Fu Chai,

Xu Chang,

Yingliang Lin

et al.

Gene, Journal Year: 2024, Volume and Issue: 908, P. 148289 - 148289

Published: Feb. 13, 2024

Language: Английский

Citations

5
MiR-223 within neutrophil axis promotes Th17 expansion by PI3K-AKT pathway in systemic lupus erythematosus DOI Creative Commons
Cheng‐Zhong Zhang, Yan Lu

Arthritis Research & Therapy, Journal Year: 2025, Volume and Issue: 27(1)

Published: Feb. 3, 2025

Further investigation is required to determine the etiology of systemic lupus erythematosus (SLE). The aim this study assess presence miR-223 within neutrophils in SLE and investigate its impact on expansion Th17 cells. Experiments were performed MRL/lpr mice, which divided into control knockdown (miR-223-) group. We assessed expression mice patients with using RT-PCR, luciferase reporter assay, Elisa, flow cytometry analysis. Signaling pathway, RT-PCR western blot conducted elucidate mechanism by expands Th17. initially identified as a pivotal factor pathogenesis both patients. Subsequently, led significant reduction mice. Moreover, inhibition effectively attenuated recruitment activation SLE. Furthermore, we found rb6-8c5 treatment alleviated symptoms reduce level Finally, elucidated that potentiate induction through thePI3K-AKT pathway mediated during SLE-associated expansion. MiR-223 neutrophil axis contributes PI3K-AKT SLE, could be therapeutic target

Language: Английский

Citations

0
Biomarkers in Systemic Lupus Erythematosus along with Metabolic Syndrome DOI Open Access
Fernanda-Isadora Corona-Meraz, Mónica Vázquez-Del Mercado,

Flavio Sandoval-García

et al.

Journal of Clinical Medicine, Journal Year: 2024, Volume and Issue: 13(7), P. 1988 - 1988

Published: March 29, 2024

Metabolic syndrome (MetS) is a group of physiological abnormalities characterized by obesity, insulin resistance (IR), and hypertriglyceridemia, which carry the risk developing cardiovascular disease (CVD) type 2 diabetes (T2D). Immune metabolic alterations have been observed in MetS are associated with autoimmune development. Systemic lupus erythematosus (SLE) an caused complex interaction environmental, hormonal, genetic factors hyperactivation immune cells. Patients SLE high prevalence MetS, elevated CVD observed. Among efforts multidisciplinary healthcare teams to make early diagnosis, wide variety considered generation biomarkers. This review aimed elucidate some primary biomarkers propose set assessments improve projection diagnosis evolution patients. These include profiles, cytokines, tests, microRNAs (miRs), be dysregulated these patients outcomes.

Language: Английский

Citations

3
Sex bias in immune response: it is time to include the sex variable in studies of autoimmune rheumatic diseases DOI Open Access
Lazaros I. Sakkas, Ian C. Chikanza

Rheumatology International, Journal Year: 2023, Volume and Issue: 44(2), P. 203 - 209

Published: Sept. 16, 2023

Language: Английский

Citations

6
Role of exosomal noncoding RNA in esophageal carcinoma DOI Creative Commons

Mao-Yan Si,

Dingyu Rao,

Yao Xia

et al.

Frontiers in Oncology, Journal Year: 2023, Volume and Issue: 13

Published: May 9, 2023

Esophageal cancer is a common malignant tumor with high degree of malignancy. Understanding its pathogenesis and identifying early diagnostic biomarkers can significantly improve the prognosis esophageal patients. Exosomes are small double-membrane vesicles found in various body fluids containing components (DNA, RNA, proteins) that mediate intercellular signal communication. Non-coding RNAs class gene transcription products encode polypeptide functions widely detected exosomes. There growing evidence exosomal non-coding involved growth, metastasis angiogenesis, also be used as prognostic markers. This article reviews recent progress cancer, including research progress, value, proliferation, migration, invasion, drug resistance, provide new ideas for precise treatment cancer.

Language: Английский

Citations

3
MiR-191-5p inhibits KLF6 to promote epithelial-mesenchymal transition in breast cancer DOI
Ling Pan,

Wenya Liu,

Hao Zhao

et al.

Technology and Health Care, Journal Year: 2023, Volume and Issue: 31(6), P. 2251 - 2265

Published: Aug. 4, 2023

MicroRNAs (miRNAs) exert certain functions in the development of several cancers and can be a potential hallmark for cancer diagnosis prognosis. MiR-191-5p has been proven to have high expression breast (BC), while its biological role regulatory mechanisms BC remain an open issue.Bioinformatics was utilized assay miR-191-5p level tissues predict downstream target gene as well enriched signaling pathways gene.qRT-PCR carried out KLF6 levels cells blood-derived exosomes from patients. Western blot examine proteins linked with cell adhesion, epithelial-mesenchymal transition (EMT), exosome markers. A dual luciferase reporter verify interaction between KLF6. Abilities phenotypes were detected by CCK8, Transwell, adhesion assay, separately.Upregulated downregulated observed cells. There targeting relationship negatively regulated promote EMT malignant progression Additionally, we described dramatically blood patients.MiR-191-5p advances KLF6, indicating that may new biomarkers BC.

Language: Английский

Citations

3
The RNA-Binding and RNA-Melting Activities of the Multifunctional Protein Nucleobindin 1 DOI Open Access
Alisa Mikhaylina,

Arina Svoeglazova,

Elena Stolboushkina

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(7), P. 6193 - 6193

Published: March 24, 2023

Nucleobindin 1 (NUCB1) is a ubiquitous multidomain protein that belongs to the EF-hand Ca2+-binding superfamily. NUCB1 interacts with Galphai3 protein, cyclooxygenase, amyloid precursor and lipids. It involved in stress response human diseases. In addition, this transcription factor binds DNA E-box motif. Using surface plasmon resonance molecular beacon approaches, we first showed RNA binding melting activities of NUCB1. We suggest could induce local changes structured RNAs via GGAUAU loop sequence. Our results demonstrate importance structure for its RNA-chaperone activity vitro.

Language: Английский

Citations

2