Identifying the HIV-Resistance-Related Factors and Regulatory Network via Multi-Omics Analyses DOI Open Access

Xueyan Long,

Gexin Liu, Xinyi Liu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11757 - 11757

Published: Nov. 1, 2024

For research on HIV/AIDS, it is important to elucidate the complex viral-host interaction, host dependency factors (HDFs), and restriction factors. However, regulatory network of HIV-resistance-related remains not well understood. Therefore, we integrated four publicly available HIV-related transcriptome datasets, along with three datasets HIV-infection-related DNA methylation, miRNA, ChIP-seq, predict influencing HIV resistance infection. Our approach involved differential analysis, functional annotation, protein-protein interaction analysis. Through comprehensive analyses, identified 25 potential genes (including shared

Language: Английский

The Role of MicroRNAs in HIV Infection DOI Open Access
Nicolás Morando, Mara Cecília Rosenzvit, María A. Pando

et al.

Genes, Journal Year: 2024, Volume and Issue: 15(5), P. 574 - 574

Published: April 29, 2024

MicroRNAs (miRNAs), a class of small, non-coding RNAs, play pivotal role in regulating gene expression at the post-transcriptional level. These regulatory molecules are integral to many biological processes and have been implicated pathogenesis various diseases, including Human Immunodeficiency Virus (HIV) infection. This review aims cover current understanding multifaceted roles miRNAs assume context HIV infection pathogenesis. The discourse is structured around three primary focal points: (i) elucidation mechanisms through which regulate replication, encompassing both direct targeting viral transcripts indirect modulation host factors critical for replication; (ii) examination miRNA by HIV, mediated either proteins or activation cellular pathways consequent infection; (iii) assessment impact on immune response progression disease HIV-infected individuals. Further, this delves into potential utility as biomarkers therapeutic agents infection, underscoring challenges prospects inherent line inquiry. synthesis evidence positions significant modulators host-virus interplay, offering promising avenues enhancing diagnosis, treatment, prevention

Language: Английский

Citations

9
MicroRNAs in HIV infection: dual regulators of viral replication and host immunity DOI
Reda Mansour, Gharieb S. El‐Sayyad,

Nehal I. Rizk

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

Language: Английский

Citations

1
Multi-omics in HIV: searching insights to understand immunological non-response in PLHIV DOI Creative Commons

Sonia Espineira,

Marina Flores-Piñas, Sílvia Chafino

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: Aug. 15, 2023

Antiretroviral therapy (ART) induces persistent suppression of HIV-1 replication and gradual recovery T-cell counts, consequently, morbidity mortality from HIV-related illnesses have been significantly reduced. However, in approximately 30% people living with HIV (PLHIV) on ART, CD4+ counts fail to normalize despite ART complete viral load, resulting severe immune dysfunction, which may represent an increased risk clinical progression AIDS non-AIDS events as well mortality. These patients are referred "immune inadequate responders", "immunodiscordant responders" or nonresponders (INR)". The molecular mechanisms underlying poor still unclear. In this sense, the use omics sciences has shed light possible factors involved activity metabolic dysregulation cells during failure INR. Moreover, identification key molecules by approaches allows for proposal potential biomarkers therapeutic targets improve quality life these patients. Hence, review aimed summarize information obtained through different concerning pathways associated INR phenotype better understand complexity immunological status infection.

Language: Английский

Citations

10
Anti-HIV-1 Effect of the Fluoroquinolone Enoxacin and Modulation of Pro-Viral hsa-miR-132 Processing in CEM-SS Cells DOI Creative Commons
Verena Schlösser, Helen L. Lightfoot,

Christine Leemann

et al.

Non-Coding RNA, Journal Year: 2025, Volume and Issue: 11(1), P. 8 - 8

Published: Jan. 20, 2025

Background: Despite tremendous advances in antiretroviral therapy (ART) against HIV-1 infections, no cure or vaccination is available. Therefore, discovering novel therapeutic strategies remains an urgent need. In that sense, miRNAs and miRNA therapeutics have moved intensively into the focus of recent HIV-1-related investigations. A strong reciprocal interdependence has been demonstrated between infection changes intrinsic cellular milieu. This interrelationship may direct potential alterations host cells’ environment beneficial for virus its suppression replication. Whether this tightly balanced controlled battle can be exploited therapeutically to further addressed. context, fluoroquinolone antibiotic Enoxacin as a potent modulator processing. Here, we test hypothesis applies also selected miRNAs. Methods: We studied effect on replication coupled with qRT-PCR analysis CEM-SS MT-4 T-cells. The effects mimic transfections combined treatment were assessed. Finally, employed vitro DICER1 cleavage assay study pro-HIV-1 hsa-miR-132 Results: established Enoxacin, but not structurally similar compound nalidixic acid, exhibits anti-HIV-1 T-cell line CEM-SS, MT-4. provide experimental data partly attributed specific downregulation mature hsa-miR-132-3p, other tested pro- miRNAs, which likely due affecting Conclusions: Our findings show anti-retroviral activity at least part by relevant future antiviral applications modulation RNA interference pathway.

Language: Английский

Citations

0
Pathogenesis of HIV-associated depression: contributing factors and underlying mechanisms DOI Creative Commons
Silvère D. Zaongo,

W.K. Wu,

Yaokai Chen

et al.

Frontiers in Psychiatry, Journal Year: 2025, Volume and Issue: 16

Published: April 17, 2025

Cumulative evidence indicates that compared to HIV negative individuals, people living with (PLWH) have a higher likelihood of developing depression, anxiety, and cognitive disorders. Depression, which is known be persistent overwhelming feeling sadness accompanied by loss interest in usual activities, one the most common mental illnesses encountered during infection. Experts believe several factors such as neuroinflammation, life stressors, lack sleep, poor nutritional state, opportunistic infections comorbidities, medications are contributing favoring development depression PLWH. However, fundamental mechanisms underlie involvement these emergence context remain poorly explored. Past researches describing role or two preceding do exist; however, very few articles tackle this important topic while considering different putative causative comprehensively particular Herein, we elaborate on currently understood responsible for discuss whereby each factor may result outcome depression. We understanding their underlying essential future therapeutic interventions alleviate burden commonly seen PLWH, therefore facilitate strategies improve overall quality life.

Language: Английский

Citations

0
Harnessing miRNA Dynamics in HIV-1-Infected Macrophages: Unveiling New Targeted Therapeutics using Systems Biology DOI Creative Commons

R Harshithkumar,

M. L. H. Kaul,

Madhuri Chandane-Tak

et al.

Computational and Structural Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Citations

0
Longitudinal analysis of hsa-miR-3163, hsa-miR-124-3p, hsa-miR-548c-3p, and hsa-miR-27a-3p as prognostic biomarkers in HIV-infected patients DOI Creative Commons
İ̇lker İnanç Balkan,

Andleeb Shahzadi,

Haktan Sönmez

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: May 6, 2025

Introduction MicroRNAs (miRNAs), key regulators of cellular pathways, play crucial roles in the pathogenesis various diseases, including Human Immunodeficiency Virus (HIV). This study aimed to evaluate expression and diagnostic potential silico -identified miRNAs (miR-124-3p, miR-27a-3p, miR-548ac-3p, miR-3163) before during antiretroviral treatment (ART), together with their correlations immunological markers (CD4 count, CD4/CD45 ratio) virological parameters (HIV RNA load). Methods Blood samples clinical data 16 patients were collected at 4 different time points; initiation ART (baseline), 1 st , 2 nd 6 th months following HIV diagnosis. healthy controls enrolled this study. RT-qPCR ELISA techniques used analyze miRNA levels while count assessed by flow cytometry. Results miR-27a-3p was significantly increased (p<0.001). miR-548ac-3p upregulated month compared individuals ART-naive subjects (p<0.05). miR-124-3p elevated comparison Conversely, miR-3163 downregulated ART-naive, 1-month, 2-month groups (p<0.001), but returned normal months. showed moderate-to-strong positive CD4 counts (R=0.46, R=0.67; p<0.001). ROC analysis identified as a promising prognostic marker, an AUC 0.8561, (95% CI: 0.756–0.9265). Discussion Our findings highlight robust biomarker for monitoring progression optimizing strategies. Validation larger cohorts is warranted confirm its utility.

Language: Английский

Citations

0
The potential emerging role of piRNA/PIWI complex in virus infection DOI
Yanyan Li, Kai Wang, Wen Liu

et al.

Virus Genes, Journal Year: 2024, Volume and Issue: 60(4), P. 333 - 346

Published: June 4, 2024

Language: Английский

Citations

3
Interactions between HIV proteins and host restriction factors: implications for potential therapeutic intervention in HIV infection DOI Creative Commons
Farooq Rashid, Silvère D. Zaongo,

Hifza Iqbal

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 16, 2024

Different host proteins target different HIV and antagonize their functions, depending on the stage of life cycle infection. Concurrently, also various to facilitate replication within cells. The preceding quite specific area knowledge in pathogenesis, however, remains insufficiently understood. We therefore propose, this review article, examine discuss that counteract those restriction which results directly increased infectivity HIV. elaborate cellular promote replication, thus functions mechanisms via Nef, Vif, Vpu, Env, Vpr, Vpx such as Ser5, PSGL-1, IFITMS, A3G, tetherin, GBP5, SAMHD1, STING, HUSH, REAF, TET2 increase infectivity. Nef antagonizes three proteins, viz., PSGL1, IFITIMs, while factors, HUSH complex; therefore, these may be potential candidates for therapeutic intervention Tetherin is targeted by Vpu PSGL1 Ser5 Env proteins. Finally, conclusive remarks future perspectives are presented.

Language: Английский

Citations

3
Human and Viral microRNA Expression in Acute and Chronic HIV Infections DOI Creative Commons
Elisabetta Lazzari, Gabriella Rozera, Roberta Gagliardini

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(4), P. 496 - 496

Published: March 23, 2024

Human and viral microRNAs (miRNAs) are involved in the regulation of gene transcription, establishment their profiles acute (AHI) chronic (CHI) HIV infections may shed light on pathogenetic events related to different phases disease. Next-generation sequencing (NGS) miRNA libraries was performed, reads were used analyze differential expression plasma with AHI CHI. Functional analysis then undertaken investigate biological processes characterizing two infection. Except for hsa-miR-122-5p, which found 3.39% vs. 0.18% CHI, most represented human miRNAs similarly However, when considering overall detected 15 displayed (FDR p < 0.05). identified 163 target mRNAs promoting angiogenesis activation versus CHI through action hsa-miR10b-5p, hsa-miR1290, hsa-miR1-3p, hsa-miR296-5p. The detected, all belonging herpesviruses, accounted only 0.014% total reads. present data suggest that patients exhibit strong innate immune upregulation hsa-miR-122-5p early angiogenesis. More specific investigations needed study role pathogenesis.

Language: Английский

Citations

2