Circulating MicroRNAs Related to Arterial Stiffness in Adults with HIV Infection DOI Creative Commons
Sideris Nanoudis, Maria P. Yavropoulou, Οlga Tsachouridou

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(12), P. 1945 - 1945

Published: Dec. 19, 2024

People with HIV (PWH) have an elevated risk of cardiovascular disease compared to those without HIV. This study aimed investigate the relative serum expression microRNAs (miRNAs) associated arterial stiffness, a significant marker disease. A total 36 male PWH and people HIV, matched for age, body mass index, pack years, dyslipidemia, were included in study. Participants history hypertension, diabetes mellitus, disease, cancer, or intravenous drug use excluded. Markers including carotid–femoral pulse wave velocity (cfPWV) augmentation index adjusted 75 beats per minute (AIx@75), measured via applanation tonometry. We analyzed 11 circulating miRNAs using real-time PCR: let-7b-5p, miR-19b-3p, miR-21-5p, miR-29a-3p, miR-126-3p, miR-130a-3p, miR-145-5p, miR-181b-5p, miR-221-3p, miR-222-3p, miR-223-3p. cfPWV was significantly higher (9.3 vs. 8.6 m/s, p = 0.019), while AIx@75, peripheral, aortic blood pressures did not differ among groups. The controls let-7b-5p (fold change: 5.24, 0.027), miR-21-5p 3.41, < 0.001), miR-126-3p 1.23, miR-222-3p 3.31, 0.002). Conversely, miR-19b-3p lower 0.61, 0.049). Among HIV-related factors, nadir CD4+T-cell count <200 cells/mm3 independently (β 0.344, 0.049), current non-nucleoside reverse transcriptase inhibitor (NNRTI) treatment 0.389, 0.010). No associations found between duration infection ART miRNA expression. highlights distinct profile which may contribute increased stiffness.

Language: Английский

Critical Role of Cellular microRNAs in Virus Infection: Decades of Progress DOI Creative Commons
Yaqi Han, Guoqing Zhang,

Xinru Lv

et al.

Animals and zoonoses., Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

1
Anti-HIV-1 Effect of the Fluoroquinolone Enoxacin and Modulation of Pro-Viral hsa-miR-132 Processing in CEM-SS Cells DOI Creative Commons
Verena Schlösser, Helen L. Lightfoot,

Christine Leemann

et al.

Non-Coding RNA, Journal Year: 2025, Volume and Issue: 11(1), P. 8 - 8

Published: Jan. 20, 2025

Background: Despite tremendous advances in antiretroviral therapy (ART) against HIV-1 infections, no cure or vaccination is available. Therefore, discovering novel therapeutic strategies remains an urgent need. In that sense, miRNAs and miRNA therapeutics have moved intensively into the focus of recent HIV-1-related investigations. A strong reciprocal interdependence has been demonstrated between infection changes intrinsic cellular milieu. This interrelationship may direct potential alterations host cells’ environment beneficial for virus its suppression replication. Whether this tightly balanced controlled battle can be exploited therapeutically to further addressed. context, fluoroquinolone antibiotic Enoxacin as a potent modulator processing. Here, we test hypothesis applies also selected miRNAs. Methods: We studied effect on replication coupled with qRT-PCR analysis CEM-SS MT-4 T-cells. The effects mimic transfections combined treatment were assessed. Finally, employed vitro DICER1 cleavage assay study pro-HIV-1 hsa-miR-132 Results: established Enoxacin, but not structurally similar compound nalidixic acid, exhibits anti-HIV-1 T-cell line CEM-SS, MT-4. provide experimental data partly attributed specific downregulation mature hsa-miR-132-3p, other tested pro- miRNAs, which likely due affecting Conclusions: Our findings show anti-retroviral activity at least part by relevant future antiviral applications modulation RNA interference pathway.

Language: Английский

Citations

0
Editorial for the “Non-Coding RNAs in Human Health and Disease” Special Issue DOI Open Access
Sujay Paul

Genes, Journal Year: 2025, Volume and Issue: 16(2), P. 211 - 211

Published: Feb. 9, 2025

Numerous non-coding RNA (ncRNA) species, including miRNAs, siRNAs, piRNAs, circRNAs, and lncRNAs, have displayed a substantial correlation with human diseases, may serve as prospective targets for gene therapy diagnostic biomarkers [...]

Language: Английский

Citations

0
Harnessing miRNA Dynamics in HIV-1-Infected Macrophages: Unveiling New Targeted Therapeutics using Systems Biology DOI Creative Commons

R Harshithkumar,

M. L. H. Kaul,

Madhuri Chandane-Tak

et al.

Computational and Structural Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Citations

0
Longitudinal analysis of hsa-miR-3163, hsa-miR-124-3p, hsa-miR-548c-3p, and hsa-miR-27a-3p as prognostic biomarkers in HIV-infected patients DOI Creative Commons
İ̇lker İnanç Balkan,

Andleeb Shahzadi,

Haktan Sönmez

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: May 6, 2025

Introduction MicroRNAs (miRNAs), key regulators of cellular pathways, play crucial roles in the pathogenesis various diseases, including Human Immunodeficiency Virus (HIV). This study aimed to evaluate expression and diagnostic potential silico -identified miRNAs (miR-124-3p, miR-27a-3p, miR-548ac-3p, miR-3163) before during antiretroviral treatment (ART), together with their correlations immunological markers (CD4 count, CD4/CD45 ratio) virological parameters (HIV RNA load). Methods Blood samples clinical data 16 patients were collected at 4 different time points; initiation ART (baseline), 1 st , 2 nd 6 th months following HIV diagnosis. healthy controls enrolled this study. RT-qPCR ELISA techniques used analyze miRNA levels while count assessed by flow cytometry. Results miR-27a-3p was significantly increased (p&lt;0.001). miR-548ac-3p upregulated month compared individuals ART-naive subjects (p&lt;0.05). miR-124-3p elevated comparison Conversely, miR-3163 downregulated ART-naive, 1-month, 2-month groups (p&lt;0.001), but returned normal months. showed moderate-to-strong positive CD4 counts (R=0.46, R=0.67; p&lt;0.001). ROC analysis identified as a promising prognostic marker, an AUC 0.8561, (95% CI: 0.756–0.9265). Discussion Our findings highlight robust biomarker for monitoring progression optimizing strategies. Validation larger cohorts is warranted confirm its utility.

Language: Английский

Citations

0
CRL4-DCAF1 Ubiquitin Ligase Dependent Functions of HIV Viral Protein R and Viral Protein X DOI Creative Commons

Ashley Dobransky,

Mary Root,

Nicholas Hafner

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(8), P. 1313 - 1313

Published: Aug. 17, 2024

The Human Immunodeficiency Virus (HIV) encodes several proteins that contort the host cell environment to promote viral replication and spread. This is often accomplished through hijacking of cellular ubiquitin ligases. These reprogrammed complexes initiate or enhance ubiquitination may otherwise act restrain replication. Ubiquitination target alter protein function proteasome-dependent destruction. HIV Viral Protein R (Vpr) related HIV-2 X (Vpx), engage CRL4-DCAF1 ligase complex numerous proteins. In this review we describe its interactions with Vpr Vpx. We additionally summarize targeted by association as well observed hypothesized impact on HIV.

Language: Английский

Citations

2
Characterizing Host microRNA: Virus Interactions of Orthoavulavirus javaense DOI Creative Commons
Megan C. Mears, Abhijeet Bakre

Viruses, Journal Year: 2024, Volume and Issue: 16(11), P. 1748 - 1748

Published: Nov. 7, 2024

Post-transcriptional gene regulation mediated by microRNAs (miRNAs) relies on sequence complementarity between the miRNA seed site and target transcript(s). This can completely inhibit or reduce translation into protein. We hypothesized that viruses employ complementarity/similarity with host miRNAs to increase miRNA-mediated of expression specifically during viral infection(s). In this study, we focus

Language: Английский

Citations

1
Anti-HIV-1 Effect of the Fluoroquinolone Enoxacin and Modulation of Pro-viral hsa-miR-132 Processing DOI Open Access
Verena Schlösser, Helen L. Lightfoot,

Christine Leemann

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 10, 2024

Abstract Background Despite tremendous advances in antiretroviral therapy (ART) against HIV-1 infections no cure or vaccination is available. Therefore, discovering novel therapeutic strategies remains an urgent need. In that sense, miRNAs and miRNA therapeutics have moved intensively into the focus of recent related investigations. A strong reciprocal interdependence has been demonstrated between infection changes intrinsic cellular milieu. This interrelationship may direct potential alterations host cells’ environment beneficial for virus its suppression replication. Whether this tightly balanced controlled battle can be exploited therapeutically, to further addressed. context, fluoroquinolone antibiotic Enoxacin as a potent modulator processing. Here, we test hypothesis applies also selected miRNAs. Methods We studied effect on replication coupled with qRT-PCR analysis CEM-SS MT-4 T-cells. The effects mimic transfections combined treatment were assessed. Finally, employed vitro DICER1 cleavage assay study pro-HIV-1 hsa-miR-132 Results established Enoxacin, but not structurally similar compound nalidixic acid, exhibits anti-HIV-1 T-cell line CEM-SS, MT-4. provide experimental data partly attributed specific downregulation mature hsa-miR-132-3p, other pro- miRNAs, which likely due affecting Conclusions Our findings show anti-retroviral activity at least part by relevant future antiviral applications modulation RNA interference pathway.

Language: Английский

Citations

0
Circulating MicroRNAs Related to Arterial Stiffness in Adults with HIV Infection DOI Creative Commons
Sideris Nanoudis, Maria P. Yavropoulou, Οlga Tsachouridou

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(12), P. 1945 - 1945

Published: Dec. 19, 2024

People with HIV (PWH) have an elevated risk of cardiovascular disease compared to those without HIV. This study aimed investigate the relative serum expression microRNAs (miRNAs) associated arterial stiffness, a significant marker disease. A total 36 male PWH and people HIV, matched for age, body mass index, pack years, dyslipidemia, were included in study. Participants history hypertension, diabetes mellitus, disease, cancer, or intravenous drug use excluded. Markers including carotid–femoral pulse wave velocity (cfPWV) augmentation index adjusted 75 beats per minute (AIx@75), measured via applanation tonometry. We analyzed 11 circulating miRNAs using real-time PCR: let-7b-5p, miR-19b-3p, miR-21-5p, miR-29a-3p, miR-126-3p, miR-130a-3p, miR-145-5p, miR-181b-5p, miR-221-3p, miR-222-3p, miR-223-3p. cfPWV was significantly higher (9.3 vs. 8.6 m/s, p = 0.019), while AIx@75, peripheral, aortic blood pressures did not differ among groups. The controls let-7b-5p (fold change: 5.24, 0.027), miR-21-5p 3.41, < 0.001), miR-126-3p 1.23, miR-222-3p 3.31, 0.002). Conversely, miR-19b-3p lower 0.61, 0.049). Among HIV-related factors, nadir CD4+T-cell count <200 cells/mm3 independently (β 0.344, 0.049), current non-nucleoside reverse transcriptase inhibitor (NNRTI) treatment 0.389, 0.010). No associations found between duration infection ART miRNA expression. highlights distinct profile which may contribute increased stiffness.

Language: Английский

Citations

0