CDetection.v2: One-pot assay for the detection of SARS-CoV-2

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: March 23, 2023

The ongoing 2019 coronavirus disease pandemic (COVID-19), caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and its variants, is a global public health threat. Early diagnosis identification of SARS-CoV-2 variants plays critical role in COVID-19 prevention control. Currently, the most widely used technique to detect quantitative reverse transcription real-time PCR (RT-qPCR), which takes nearly 1 hour should be performed experienced personnel ensure accuracy results. Therefore, development nucleic acid detection kit with higher sensitivity, faster greater important.Here, we optimized system components reaction conditions our previous approach using RT-RAA Cas12b.We developed Cas12b-assisted one-pot platform (CDetection.v2) that allows rapid 30 minutes. This was able up 5,000 copies/ml without cross-reactivity other viruses. Moreover, sensitivity this CRISPR comparable RT-qPCR when tested on 120 clinical samples.The CDetection.v2 provides novel based integration CRISPR/Cas12b for detecting screening large-scale samples, offering more efficient strategy various types

Language: Английский

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Variant-Related Differences in Laboratory Biomarkers among Patients Affected with Alpha, Delta and Omicron: A Retrospective Whole Viral Genome Sequencing and Hospital-Setting Cohort Study

Biomedicines, Journal Year: 2023, Volume and Issue: 11(4), P. 1143 - 1143

Published: April 10, 2023

During the COVID-19 pandemic, different SARS-CoV-2 variants of concern (VOC) with specific characteristics have emerged and spread worldwide. At same time, clinicians routinely evaluate results certain blood tests upon patient admission as well during hospitalization to assess disease severity overall status. In present study, we searched for significant cell count biomarker differences among patients affected Alpha, Delta Omicron VOCs at admission. Data from 330 were retrieved regarding age, gender, VOC, (WBC, Neut%, Lymph%, Ig%, PLT), common biomarkers (D-dimers, urea, creatinine, SGOT, SGPT, CRP, IL-6, suPAR), ICU death. Statistical analyses performed using ANOVA, Kruskal-Wallis test, two-way Chi-square, T-test, Mann-Whitney test logistic regression was where appropriate SPSS v.28 STATA 14. Age VOC significantly associated hospitalization, whereas groups found WBC, PLT, D-dimers suPAR. Our showed that throughout current not only but also laboratory parameters are used patient's status subject changes.

Language: Английский

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Characterization of anti-SARS-CoV-2 monoclonal antibodies focusing on antigen binding, neutralization, and FcγR activation via formation of immune complex

mAbs, Journal Year: 2023, Volume and Issue: 15(1)

Published: June 12, 2023

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV−2) causes disease 2019 (COVID−19). Antibodies induced by SARS-CoV−2 infection or vaccination play pivotal roles in the body's defense against virus; many monoclonal antibodies (mAbs) have been cloned, and some neutralizing mAbs used as therapeutic drugs. In this study, we prepared an antibody panel consisting of 31 clones anti-SARS-CoV−2 analyzed compared their biological activities. The study were classified into different binding classes based on epitopes showed to spike protein kinetics. A multiplex assay using proteins Alpha, Beta, Gamma, Delta, Omicron variants clearly effects variant mutations neutralization activities mAbs. addition, evaluated Fcγ receptor (FcγR) activation immune complexes mAb pseudo-typed virus, revealed differences FcγR properties among It has reported that FcγR-mediated immune-cell is involved promotion immunopathology COVID−19; therefore, FcγR-activation are most important characteristics when considering clinical impacts

Language: Английский

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Facing the Challenges in the COVID-19 Pandemic Era: From Standard Treatments to the Umbilical Cord-Derived Mesenchymal Stromal Cells as a New Therapeutic Strategy

Cells, Journal Year: 2023, Volume and Issue: 12(12), P. 1664 - 1664

Published: June 19, 2023

Coronavirus disease 2019 (COVID-19), the pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which counts more than 650 million cases and 6.6 of deaths worldwide, affects system with typical symptoms such as fever, cough, sore throat, distress (ARDS), fatigue. Other nonpulmonary manifestations are related abnormal inflammatory response, "cytokine storm", that could lead to a multiorgan death. Evolution effective vaccines against SARS-CoV-2 provided multiple options prevent infection, but treatment forms remains difficult manage. The cytokine storm is usually counteracted standard medical care anti-inflammatory drugs, researchers moved forward their studies on new strategies based cell therapy approaches. perinatal tissues, placental membranes, amniotic fluid, umbilical cord derivatives, enriched in mesenchymal stromal cells (MSCs) exert well-known role, immune response modulation, tissue repair. In this review, we focused umbilical-cord-derived MSCs (UC-MSCs) used vitro vivo order evaluate weakening symptoms, recent clinical trials from different databases, supporting favorable potential UC-MSCs therapeutic strategy.

Language: Английский

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Differential Transcriptomic Landscapes of SARS-CoV-2 Variants in Multiple Organs from Infected Rhesus Macaques

Genomics Proteomics & Bioinformatics, Journal Year: 2023, Volume and Issue: 21(5), P. 1014 - 1029

Published: July 13, 2023

Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused the persistent disease 2019 (COVID-19) pandemic, which has resulted in millions of deaths worldwide and brought an enormous public health global economic burden. The recurring wave infections been exacerbated by growing variants SARS-CoV-2. In this study, virological characteristics original SARS-CoV-2 strain its concern (VOCs; including Alpha, Beta, Delta) vitro, as well differential transcriptomic landscapes multiple organs (lung, right ventricle, blood, cerebral cortex, cerebellum) from infected rhesus macaques, were elucidated. a stronger innate immune response host cells, VOCs markedly increased levels subgenomic RNA s, such N, Orf9b, Orf6, Orf7ab, are known antagonists inhibitors antiviral factors. Intriguingly, Alpha variant induced larger alteration abundance tissues monkeys than Beta Delta did. Moreover, hyperinflammatory state active shown ventricles up-regulation inflammation- immune-related RNAs. Furthermore, peripheral blood may mediate signaling transmission among to coordinate molecular changes individuals. Collectively, these data provide insights into pathogenesis COVID-19 at early stage infection VOCs.

Language: Английский

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