Clinical Nutrition, Journal Year: 2024, Volume and Issue: 43(6), P. 1459 - 1472
Published: April 12, 2024
Language: Английский
Molecular and Cellular Biochemistry, Journal Year: 2023, Volume and Issue: 478(12), P. 2683 - 2693
Published: March 13, 2023
Language: Английский
Citations
8
BMC Cancer, Journal Year: 2023, Volume and Issue: 23(1)
Published: May 6, 2023
Abstract We used pyroptosis-related genes to establish a risk–score model for prognostic prediction of liver hepatocellular carcinoma (LIHC) patients. A total 52 pyroptosis-associated were identified. Then, data 374 LIHC patients and 50 normal individuals acquired from the TCGA database. Through gene expression analyses, differentially expressed (DEGs) determined. The 13 (PRGs) confirmed as potential factors through univariate Cox regression analysis entered into Lasso multivariate build PRGs signature, containing four (BAK1, GSDME, NLRP6, NOD2) determined independent factors. mRNA levels evaluated by qRT-PCR, while overall survival (OS) rates assessed Kaplan–Meier method. Enrichment analyses done mechanisms associated with differential status tumor immunology perspective. Additionally, risk score could divide low- high-risk groups using median cut-off. nomogram, derived integrating clinical characteristics patients, was constructed. function also validated GEO, ICGC cohorts, online databases Plotter. Small interfering RNA-mediated knockdown well lentivirus-mediated GSDME knockdown, performed validate that markedly suppressed growth HCC cells both in vivo vitro. Collectively, our study demonstrated signature had great value prognosis assessment.
Language: Английский
Citations
8
Aging, Journal Year: 2023, Volume and Issue: 15(21), P. 12314 - 12329
Published: Nov. 7, 2023
Renal clear cell carcinoma (ccRCC) is the most common type of renal cancer. Here we aim to explore prognosis and immunotherapeutic value copper death-related gene Dihydrolipoamide S-acetyltransferase (DLAT) in ccRCC.The mRNA protein expressions methylation level DLAT, as well relation DLAT survival prognosis, clinical characteristics, biological function, immune microenvironment responses patients with ccRCC were evaluated using multiple databases. In addition, 75 paired tissue samples 3 kinds lines tested for experimental validation.Bioinformatics analysis databases, qRT-PCR, western blot verified that expression was lower than paracancerous tissues. Patients low had a rate, worse stronger infiltration immunosuppressive points, higher tumor dysfunction exclusion (TIDE) scores.DLAT identified an independent prognostic factor closely related ccRCC.
Language: Английский
Citations
8
Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)
Published: Dec. 21, 2023
Lipoyltransferase 2 (LIPT2) acts as a key enzyme involved in fatty acid metabolism and cell membrane synthesis. However, the biological function of LIPT2 various cancer types its potential significance prognosis continue to be unresolved. For this analysis, we evaluated expression levels gene all cancers by bioinformatics methods. The results found that was dramatically overexpressed vast majority cancers. upregulated related bad Brain Lower Grade Glioma (LGG), (GBMLGG), Glioblastoma multiforme (GBM), Kidney Chromophobe (KICH), High-Risk Wilms Tumor (WT), while it had favorable renal clear carcinoma (KIRC), Ovarian serous cystadenocarcinoma (OV), Pan-kidney cohort (KIPAN). Furthermore, assessed mutation status, methylation levels, immune status pan-cancer. Single-cell sequencing revealed correlation with characteristics such DNA lesion, tumor angiogenesis, apoptosis, metastasis, invasion. Enrichment analysis unveiled molecular regulatory mechanisms. In conclusion, our research reveals detailed role progression, prognosis, efficacy forms cancer. Therefore, have reason believe has candidate biomarker for tumors.
Language: Английский
Citations
7
Clinical Nutrition, Journal Year: 2024, Volume and Issue: 43(6), P. 1459 - 1472
Published: April 12, 2024
Language: Английский
Citations
2