The effect of the mechanodynamic lung environment on fibroblast phenotype via the Flexcell DOI Creative Commons

S. Al Yazeedi,

A. F Abokor,

J. Brussow

et al.

BMC Pulmonary Medicine, Journal Year: 2024, Volume and Issue: 24(1)

Published: July 27, 2024

Abstract The lung is a highly mechanical organ as it exposed to approximately 10 9 strain cycles, (where the length change of tissue structure per unit initial length), with an 4% amplitude during quiet tidal breathing or 7 cycles at 25% heavy exercises, sighs, and deep inspirations. These indices have been reported become aberrant in diseases such acute respiratory distress syndrome (ARDS), pulmonary hypertension, bronchopulmonary dysplasia (BPD), idiopathic fibrosis (IPF), asthma. Through recent innovations, various vitro systems/bioreactors used mimic lung’s developed. Among these, Flexcell tension system which composed bioreactors that utilize variety programs apply static cyclic on different cell-types established 2D monolayer cultures cell-embedded 3D hydrogel models, has enabled assessment response cells fibroblasts health disease. Fibroblasts are main effector responsible for production extracellular matrix (ECM) proteins repair maintain homeostasis implicated excessive deposition leads fibrosis. In this review, we summarise, studies bioreactor assess effects structure, function, phenotype homeostatic conditions abnormal environments associated injury We show these revealed regulate fibroblast proliferation, ECM protein production, inflammation normal diseased lung.

Language: Английский

Copaiba oil minimizes inflammation and promotes parenchyma re-epithelization in acute allergic asthma model induced by ovalbumin in BALB/c mice DOI Creative Commons

Ludmila de Souza Caputo,

Carolina de Lima Alves,

Inês Martins Laranjeira

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: April 11, 2024

Introduction: Asthma is a condition of airflow limitation, common throughout the world, with high mortality rates, especially as it still faces some obstacles in its management. As constitutes public health challenge, this study aimed to investigate effect copaiba oil (e.g., Copaifera langsdorffii ), treatment resource, at doses 50 and 100 mg/kg on certain mediators acute lung inflammation (IL-33, GATA3, FOXP3, STAT3, TBET) early mechanisms remodeling (degradation elastic fiber tissues, collagen deposition, goblet cell hyperplasia). Methods: Using an ovalbumin-induced allergic asthma model BALB/c mice, we analyzed inflammatory through immunohistochemistry histopathology, employing orcein, Masson’s trichrome, periodic acid-Schiff staining. Results: Copaiba (CO) reduced IL-33 increased FOXP3 by stimulating FOXP3/GATA3 FOXP3/STAT3 pathways. Additionally, upregulated TBET, suggesting additional role controlling GATA3 activity. In respiratory epithelium, CO decreased fragmentation fibers while increasing deposition fibers, favoring epithelial restructuring. Simultaneously, hyperplasia. Discussion: Although research warranted, demonstrated anti-inflammatory re-epithelializing action makes viable option exploring new treatments for asthma.

Language: Английский

Citations

1
Review of in-vitro studies evaluating respiratory toxicity of aerosols: impact of cell types, chemical composition, and atmospheric processing DOI
Sudheer Salana, Vishal Verma

Environmental Science Processes & Impacts, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

In recent decades, several cell-based and acellular methods have been developed to evaluate ambient particulate matter (PM) toxicity. Although provide a more comprehensive assessment of PM toxicity, their results are difficult comprehend due the diversity in cellular endpoints, cell types, assays interference chemical components with some assays' techniques. this review, we attempt clarify these issues. We first discuss morphological immunological differences among various macrophage epithelial cells, belonging respiratory systems human murine species, used

Language: Английский

Citations

1
Integrative Cross-Talk in Asthma: Unraveling the Complex Interactions Between Eosinophils, Immune, and Structural Cells in the Airway Microenvironment DOI Creative Commons
Andrius Januskevicius,

Egle Vasyle,

Airidas Rimkūnas

et al.

Diagnostics, Journal Year: 2024, Volume and Issue: 14(21), P. 2448 - 2448

Published: Oct. 31, 2024

Asthma is a chronic inflammatory process that leads to airway narrowing, causing breath loss followed by spasms, wheezing, and shortness of breath. Within the asthmatic lungs, interaction among various immune cells structural plays significant role in orchestrating an response which eosinophils hold central importance. In these settings, allergens or other environmental exposures commonly drive recruit airways. The appearance airways indicates dynamic interplay cell types within lung tissue does not represent passive effect inflammation. cellular cross-talk causes persistence eosinophilic inflammation, if left untreated, it results long-term damage structure function. Further exacerbation condition occurs because this. We discuss how this complex eosinophils, immune, microenvironment distinct pathophysiological features asthma, variability disease severity, biological treatments.

Language: Английский

Citations

1
Potential of Patient-Derived Organoids and Other 3D Culture Models to Assess Immunotherapy Response in Cancer DOI
Melissa Acosta-Plasencia,

Philipp Menauer,

Ramón M. Marrades

et al.

Springer eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 29

Published: Jan. 1, 2024

Language: Английский

Citations

0
Asthma in patients with the syndrome of undifferentiated dysplasia of connective tissue: peculiarities of the course or mutually aggravating mechanisms? DOI
Олена СОЛЄЙКО,

Mariia O. Chernykh,

І. А. Iliuk

et al.

Wiadomości Lekarskie, Journal Year: 2024, Volume and Issue: 77(4), P. 821 - 827

Published: Jan. 1, 2024

Aim: To analyse laboratory and biochemical features of the severe persistent course asthma in patients with undifferentiated connective tissue dysplasia (UCTD) syndrome, their phenotypic visceral stigmas dysembryogenesis. Materials Methods: We enrolled 60 male asthma, aged from 23 to 62 years (mean age (46.83 ±0.85) years): 30 background UCTD, - without UCTD. analysed clinical, somatometric, surveying (original questionnaire based on map Glesby), instrumental (spirography, echocardiography, endoscopy, esophagofibrogastroduodenoscopy) (including eosinophilic granulocytes aldosterone levels) data. Results: Correlations were found men UCTD between number markers rate earlobe diagonal fold (r=+0.75; р<0.05), asthenic constitution (r=+0.72; easy bruising (r=+0.7; p<0.05) straight abdominal line hernia (r=+0.52; p<0.05). Average serum level (176,10 ±11,22) was significantly higher than those (142,77 ±± 9,43), (p<0.05), as well average eosinophils levels (1.3 ±0.25 vs. 0.57 ±0.12, In absolute majority (93.3%) onset confirmed after pneumonia, manifestation (37.2 ±1.21) (21.4 ±1.13). Also, there a high exacerbations during last year (2.7 ±0.12 per year) doses combined inhaled glucocorticosteroids use. Conclusions: Identified “phenotypic profile”, clinical which determine early formation complications, will further accelerate diagnosis this phenotype improve approaches selection treatment regimens for these patients.

Language: Английский

Citations

0
The effect of the mechanodynamic lung environment on fibroblast phenotype via the Flexcell DOI Creative Commons

S. Al Yazeedi,

A. F Abokor,

J. Brussow

et al.

BMC Pulmonary Medicine, Journal Year: 2024, Volume and Issue: 24(1)

Published: July 27, 2024

Abstract The lung is a highly mechanical organ as it exposed to approximately 10 9 strain cycles, (where the length change of tissue structure per unit initial length), with an 4% amplitude during quiet tidal breathing or 7 cycles at 25% heavy exercises, sighs, and deep inspirations. These indices have been reported become aberrant in diseases such acute respiratory distress syndrome (ARDS), pulmonary hypertension, bronchopulmonary dysplasia (BPD), idiopathic fibrosis (IPF), asthma. Through recent innovations, various vitro systems/bioreactors used mimic lung’s developed. Among these, Flexcell tension system which composed bioreactors that utilize variety programs apply static cyclic on different cell-types established 2D monolayer cultures cell-embedded 3D hydrogel models, has enabled assessment response cells fibroblasts health disease. Fibroblasts are main effector responsible for production extracellular matrix (ECM) proteins repair maintain homeostasis implicated excessive deposition leads fibrosis. In this review, we summarise, studies bioreactor assess effects structure, function, phenotype homeostatic conditions abnormal environments associated injury We show these revealed regulate fibroblast proliferation, ECM protein production, inflammation normal diseased lung.

Language: Английский

Citations

0