Discovery of Glucose Metabolism-Associated Genes in Neuropathic Pain: Insights from Bioinformatics DOI Open Access

Ying Yu,

R. Cheung,

Chi-Wai Cheung

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13503 - 13503

Published: Dec. 17, 2024

Metabolic dysfunction has been demonstrated to contribute diabetic pain, pointing towards a potential correlation between glucose metabolism and pain. To investigate the relationship altered neuropathic we compared samples from healthy subjects with those intervertebral disc degeneration (IVDD) patients, utilizing data two public datasets. This led identification of 412 differentially expressed genes (DEG), which 234 were upregulated 178 downregulated. Among these, three key (Ins, Igfbp3, Plod2) found. Kyoto Encyclopedia Genes Genomes pathway analysis enrichment hub in pathways such as positive regulation ErbB signaling pathway, monocyte activation, response reactive oxygen species; thereby suggesting these biological pain sensation. Further identified Plod2), showed significant correlations immune cell infiltration, their roles modulating through response. validate our findings, quantitative real-time polymerase chain reaction (qPCR) confirmed expression levels partial sciatic nerve ligation (PSNL) model, immunofluorescence studies increased infiltration at injury site. Behavioral assessments further corroborated hypersensitivity (NP) models. Our study sheds light on molecular mechanisms underlying NP aids therapeutic targets for future drug development.

Language: Английский

Comprehensive analysis of PRPF19 immune infiltrates, DNA methylation, senescence-associated secretory phenotype and ceRNA network in bladder cancer DOI Creative Commons
Yaxuan Wang, Jinfeng Wang,

JiaXing He

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: Nov. 6, 2023

Background Pre-mRNA processing factor 19 (PRPF19) is an E3 ligase that plays a crucial role in repairing tumor-damaged cells and promoting cell survival. However, the predictive value biological function of PRPF19 bladder urothelial carcinoma (BLCA) require further investigation. Methods In this study, we utilized transcriptomic data cancer tissue microarrays to identify high expression BLCA, suggesting its potential as prognostic biomarker. To gain better understanding immune microenvironment performed single analysis employed LASSO method. Additionally, examined methylation profiles using SMART website. Our investigation confirmed correlation between BLCA senescence stemness. Furthermore, constructed PRPF19-miR-125a-5p-LINC02693-MIR4435-2HG ceRNA network ENCORI miRWALK databases. Results comprehensive reveals can serve marker for significantly associated with various immune-infiltrating BLCA. Moreover, our findings suggest influences cellular through regulation Finally, developed has predict prognosis patients. Conclusion We multiple functions specific be used therapeutic target

Language: Английский

Citations

23
YTHDC1‐dependent m6A modification modulated FOXM1 promotes glycolysis and tumor progression through CENPA in triple‐negative breast cancer DOI Creative Commons
Xi Shen, Jianxin Zhong, Yu Pan

et al.

Cancer Science, Journal Year: 2024, Volume and Issue: 115(6), P. 1881 - 1895

Published: April 3, 2024

Abstract Triple‐negative breast cancer (TNBC) exhibits heightened aggressiveness compared with other (BC) subtypes, earlier relapse, a higher risk of distant metastasis, and worse prognosis. Transcription factors play pivotal role in various cancers. Here, we found that factor forkhead box M1 (FOXM1) expression was significantly TNBC than BC subtypes normal tissues. Combining the findings Gene Ontology (GO) enrichment analysis series experiments, knockdown FOXM1 gene attenuated ability cells to proliferate metastasize both vivo vitro. In addition, Spearman's test showed correlated glycolysis‐related genes, especially centromere protein A (CENPA) datasets (GSE76250, GSE76124, GSE206912, GSE103091). The effect silencing on inhibition CENPA expression, proliferation, migration, glycolysis could be recovered by overexpression CENPA. According MeRIP, level m6A modification FOMX1 decreased treated cycloleucine (a inhibitor) control group. increase caused YTHDC1 reversed inhibitor, which indicated enhanced depending modification. Therefore, concluded YTHDC1‐m6A modification/FOXM1/CENPA axis plays an important progression glycolysis.

Language: Английский

Citations

9
FoxO1 promotes ovarian cancer by increasing transcription and METTL14‐mediated m6A modification of SMC4 DOI Creative Commons
Liping Tan,

Shuangan Wang,

Shijia Huang

et al.

Cancer Science, Journal Year: 2024, Volume and Issue: 115(4), P. 1224 - 1240

Published: Feb. 25, 2024

The transcription factor forkhead box protein O1 (FoxO1) is closely related to the occurrence and development of ovarian cancer (OC), however its role molecular mechanisms remain unclear. Herein, we found that FoxO1 was highly expressed in clinical samples OC patients significantly correlated with poor prognosis. knockdown inhibited proliferation cells vitro vivo. ChIP-seq combined GEPIA2 Kaplan-Meier database analysis showed structural maintenance chromosome 4 (SMC4) a downstream target FoxO1, promotes SMC4 by binding -1400/-1390 bp promoter. high expression blocked tumor inhibition effect knockdown. Furtherly, increased mRNA abundance transcriptionally activating methyltransferase-like 14 (METTL14) increasing m

Language: Английский

Citations

6
A systematic overview of single-cell transcriptomics databases, their use cases, and limitations DOI Creative Commons

Mahnoor N. Gondal -,

Syed Hamad Hassan Shah, Arul M. Chinnaiyan

et al.

Frontiers in Bioinformatics, Journal Year: 2024, Volume and Issue: 4

Published: July 8, 2024

Rapid advancements in high-throughput single-cell RNA-seq (scRNA-seq) technologies and experimental protocols have led to the generation of vast amounts transcriptomic data that populates several online databases repositories. Here, we systematically examined large-scale scRNA-seq databases, categorizing them based on their scope purpose such as general, tissue-specific disease-specific cancer-focused cell type-focused databases. Next, discuss technical methodological challenges associated with curating along current computational solutions. We argue understanding including limitations assumptions, is crucial for effectively utilizing this make robust discoveries identify novel biological insights. Such platforms can help bridge gap between wet lab scientists through user-friendly web-based interfaces needed democratizing access data. These would facilitate interdisciplinary research, enabling researchers from various disciplines collaborate effectively. This review underscores importance leveraging approaches unravel complexities offers a promising direction future research field.

Language: Английский

Citations

6
KIAA1429 increases FOXM1 expression through YTHDF1–mediated m6A modification to promote aerobic glycolysis and tumorigenesis in multiple myeloma DOI Creative Commons

Yue Wu,

Y. Luo,

Xingchen Yao

et al.

Cell Biology and Toxicology, Journal Year: 2024, Volume and Issue: 40(1)

Published: July 26, 2024

Abstract Objective Multiple myeloma (MM) is a deadly plasma cell malignancy with elusive pathogenesis. N6-methyladenosine (m6A) critically engaged in hematological malignancies. The function of KIAA1429, the largest component methyltransferases, unknown. This study delved into mechanism KIAA1429 MM, hoping to offer novel targets for MM therapy. Methods Bone marrow samples were attained from 55 patients and 15 controls. YTHDF1, FOXM1 mRNA levels detected their correlation was analyzed. Cell viability, proliferation, cycle, apoptosis testified. Glycolysis-enhancing genes (HK2, ENO1, LDHA), lactate production, glucose uptake evaluated. interaction between m6A-modified level, stability assayed. A transplantation tumor model built confirm KIAA1429. Results at high cells linked poor prognoses. knockdown restrained arrested G0/G1 phase, increased apoptosis. positively glycolysis-enhancing genes. genes, uptake, production repressed after knockdown, along reduced stability. YTHDF1 recognized KIAA1429-methylated raised Knockdown curbed aerobic glycolysis malignant behaviors cells, which nullified by overexpression. also inhibited growth animal experiments. Conclusion reduces expression through YTHDF1-mediated m6A modification, thus inhibiting tumorigenesis. Graphical tumorigenesis

Language: Английский

Citations

5
miR-125 in Breast Cancer Etiopathogenesis: An Emerging Role as a Biomarker in Differential Diagnosis, Regenerative Medicine, and the Challenges of Personalized Medicine DOI Creative Commons
Roberto Piergentili, Enrico Marinelli, Gaspare Cucinella

et al.

Non-Coding RNA, Journal Year: 2024, Volume and Issue: 10(2), P. 16 - 16

Published: Feb. 21, 2024

Breast Cancer (BC) is one of the most common cancer types worldwide, and it characterized by a complex etiopathogenesis, resulting in an equally classification subtypes. MicroRNA (miRNA or miR) are small non-coding RNA molecules that have essential role gene expression significantly linked to tumor development angiogenesis different cancer. Recently, interactions among coding been elucidated, further shedding light on complexity roles these fulfill formation. In this context, knowledge about miR BC has improved, highlighting deregulation as additional factors influencing occurrence, classification. A considerable number papers published over past few years regarding miR-125 human pathology general several formation particular. Interestingly, family members recently well, (competing endogenous networks, ceRNET) between molecule target mRNA described. review, we summarize state-of-the-art research topic.

Language: Английский

Citations

4
The oncogenic role of FOXM1 in hepatocellular carcinoma: molecular mechanisms, clinical significance, and therapeutic potentials DOI
Fengtian He, He Liu, Fangcheng Zhao

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: April 23, 2025

Language: Английский

Citations

0
FOXM1 transcriptional regulation DOI
Mengxi Li,

Xuzheng Gao,

Yanting Su

et al.

Biology of the Cell, Journal Year: 2024, Volume and Issue: 116(9)

Published: July 4, 2024

Abstract FOXM1 is a key transcriptional regulator involved in various biological processes mammals, including carbohydrate and lipid metabolism, aging, immune regulation, development, disease. Early studies have shown that acts as an oncogene by regulating cell proliferation, cycle, migration, metastasis, apoptosis, well genes related to diagnosis, treatment, chemotherapy resistance, prognosis. Researchers are increasingly focusing on functions tumor microenvironment, epigenetics, infiltration. However, researchers not comprehensively described FOXM1's involvement microenvironment shaping, Here we review the role of formation development malignant tumors, will provide comprehensive summary interacting proteins, infiltration, suggest areas for further research.

Language: Английский

Citations

3
The impact of epitranscriptomic modifications on liver disease DOI
Keith A. Berggren, Robert E. Schwartz, Ralph E. Kleiner

et al.

Trends in Endocrinology and Metabolism, Journal Year: 2024, Volume and Issue: 35(4), P. 331 - 346

Published: Jan. 11, 2024

Language: Английский

Citations

3
CAF-EVs carry lncRNA MAPKAPK5-AS1 into hepatocellular carcinoma cells and promote malignant cell proliferation DOI Creative Commons

Lin Sheng,

Junmei Lin, Yili Zhang

et al.

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: Dec. 30, 2024

Hepatocellular carcinoma (HCC) is an aggressive malignancy with poor prognosis. LncRNA MAPKAPK5-AS1 a potential oncogene and contributes to HCC cell malignant proliferation. This study explores the role of carried by carcinoma-associated fibroblasts-derived extracellular vesicles (CAF-EVs) in Our findings reveal that CAF-EVs promotes proliferation delivering MAPKAPK5-AS1, which binds inhibits SMURF2 stabilizes TCF12. leads TCF12 ubiquitination degradation. upregulates FOXH1 expression. In animal model, enhances tumor growth stabilizing via activating transcription. conclusion, carrying expression competitively inhibiting SMURF2, thus promoting TCF12-mediated transcription driving may offer insights for treatment suggest targets future treatments, opening avenues therapies.

Language: Английский

Citations

1