Dietary strategies for early immune modulation in primary food allergy prevention DOI Creative Commons
Ralf G. Heine

BMJ Nutrition Prevention & Health, Journal Year: 2023, Volume and Issue: 6(Suppl 3), P. s8 - s19

Published: Dec. 1, 2023

As the global incidence of food allergies continues to rise, effective primary prevention strategies remain a public health priority. Eczema in early infancy is major risk factor for IgE-mediated allergies. Exposure allergens via inflamed skin promotes cascade allergic immune responses and increases By contrast, exposure gut likely induce oral tolerance. Landmark studies have demonstrated significant reduction egg peanut allergy response dietary introduction allergens. result, feeding guidelines countries with high shifted from recommending prolonged allergen avoidance policy around 6 months age, as part diverse complementary diet. Uncertainties regarding optimum timing specific allergens, well minimum frequency dose. Exclusive breast months, continuation 2 years, generally recommended but does not appear consistently prevent Perinatal intermittent supplementary cow’s milk-based formula may increase milk protein allergy. Maternal during pregnancy lactation effective. However, maternal consumption appears their preventive effect infant conjunction into Data on supplementation pregnant women, breastfeeding mothers infants vitamin D, omega-3 polyunsaturated fatty acids, prebiotics or probiotics purpose are insufficient make firm clinical recommendations. In eczema, combination regular emollient treatment additive effects, trials so far had mixed outcomes. Further combining interventions aiming restore disrupted barrier eczema provide new insights at-risk populations.

Language: Английский

Immune‐related microRNAs in breast milk and their relation to regulatory T cells in breastfed children DOI Creative Commons
Emelie Ahlberg, Magalí Martí, Dhanapal Govindaraj

et al.

Pediatric Allergy and Immunology, Journal Year: 2023, Volume and Issue: 34(4)

Published: April 1, 2023

Abstract Background The immunomodulatory capacity of breast milk may partially be mediated by microRNAs (miRNA), small RNA molecules that regulate gene expression on a post‐transcriptional level and are hypothesized to involved in modulation immunological pathways. Here, we evaluate the immune‐related miRNAs after pre‐ postnatal supplementation with Limosilactobacillus reuteri omega‐3 (ω‐3) polyunsaturated fatty acids (PUFAs), association infant regulatory T cell (Treg) frequencies. Methods One‐hundred twenty women included double‐blind, randomized, placebo‐controlled allergy intervention trial received L. and/or ω‐3 PUFAs daily from gestational week 20. Using Taqman qPCR, 24 were analyzed obtained at birth (colostrum) 3 months (mature milk) lactation. proportion activated resting Treg cells blood using flow cytometry 6, 12, months. Results Relative changed significantly over lactation period for most miRNAs; however, was not influenced any supplements. Colostrum miR‐181a‐3p correlated frequencies 6 miR‐148a‐3p let‐7d‐3p months, as did mature miR‐181c‐3p. Conclusion Maternal affect relative miRNA . Interestingly, some correlate subpopulations breastfed children, supporting hypothesis could important immune regulation. Trial registration ClinicalTrials.gov‐ID: NCT01542970.

Language: Английский

Citations

15
Expression Profile of MicroRNAs in Breast Milk of Women With Inflammatory Bowel Disease: Correlation With Disease Activity and Medical Treatments DOI Creative Commons
Regina Golan‐Gerstl, Ami Ben Ya’acov,

Mirit Musseri

et al.

Inflammatory Bowel Diseases, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 17, 2025

Although most inflammatory bowel disease (IBD) medications are considered safe during pregnancy, their impact on microRNAs (miRNAs) in breast milk is largely unknown. MiRNAs milk, carried by milk-derived extracellular vesicles (MDEs), transmitted to the newborn's gut regulate genes. Aberrant miRNA expression profiles have been found IBD within tissue, blood, and feces, but data mother's scarce. We collected samples from 32 mothers with Crohn's (CD), 14 ulcerative colitis (UC), 44 healthy controls. analyzed through qualitative real-time polymerase chain reaction Affymetrix chips. Target genes of differentially expressed miRNAs were predicted using miRATBase. Statistical analyses conducted GraphPad Prism software Mann-Whitney tests. Milk-derived showed altered compared Specifically, miR-21 miR-320 downregulated, while Let-7a was upregulated mothers. The patterns varied between CD UC, significantly lower MiR-21 UC higher CD. Additionally, anti-tumor necrosis factor treatment pregnancy associated reduced miR-148a levels MDEs. Pathway analysis revealed that these involved immune regulation, particularly interleukin signaling pathways. This study highlights IBD, influenced its treatments. These findings emphasize maternal health composition potential implications for infant development. Understanding may guide personalized strategies promote breastfeeding among IBD.

Language: Английский

Citations

0
Exosomes in Dermatological Research: Unveiling Their Multifaceted Role in Cellular Communication, Healing, and Disease Modulation DOI Creative Commons
Daniela Pinto,

Giammaria Giuliani,

Giorgia Mondadori

et al.

Cosmetics, Journal Year: 2025, Volume and Issue: 12(1), P. 16 - 16

Published: Jan. 22, 2025

Exosomes, a subtype of extracellular vehicles (EVs), play pivotal role in cellular communication and have gained considerable attention dermatological research. Formed through the inward budding endosomal membrane, exosomes facilitate transfer proteins, lipids, nucleic acids, including microRNAs (miRNAs), thereby influencing behavior function recipient cells. These vesicles are secreted by various cell types, keratinocytes, crucial for maintaining skin homeostasis, regulating immune responses, promoting wound healing. Exosomes demonstrated therapeutic potential addressing conditions such as hair disorders, cancers photoaging enhanced regeneration reduced oxidative stress. However, they also implicated disease progression, with pathogens utilizing exosome release to evade host responses. Recent studies highlight diverse origins functions exosomes, suggesting their promise innovative agents dermatology. As research continues elucidate multifaceted roles, represent frontier understanding intercellular developing novel treatments skin-related diseases, underscoring impact on both health clinical applications. This review synthesizes existing literature biology isolation focus implications contexts.

Language: Английский

Citations

0
Can exclusive breastfeeding in the first 4 months reduce food allergy? A retrospective questionnaire study DOI

Nadira Musallam,

Meital Almog,

Roni Wagner

et al.

Annals of Allergy Asthma & Immunology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

0
A Review of microRNA in Cheese: From Processing to Putative Biological Implications DOI Creative Commons
Olubukunmi Amos Ilori, Diana Marisol Abrego-Guandique, Maria Cristina Caroleo

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106290 - 106290

Published: March 1, 2025

Language: Английский

Citations

0
Key roles of milk-derived extracellular vesicles in intestinal tract, with special emphasis on microRNAs in extracellular vesicles DOI
Xue Bai,

Jingwen Shang,

Hong Yu

et al.

Critical Reviews in Food Science and Nutrition, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 23

Published: March 6, 2025

Studies have indicated that milk extracellular vesicles (EVs) are resistant to both the enzymatic activity of digestive enzymes and acidic conditions common in gastrointestinal tract. Thus, intestinal tract efficiently absorbs uses these EVs. There is mounting evidence recent years microRNAs (miRNAs) found EVs derived from essential for prebiotic effect through target gene regulation. In this paper, we summarized sources, biosynthetic processes, cargos, factors affecting milk-derived Furthermore, with particular attention potential communication mechanism cell receptors, including recognizing binding surface receptor cells, internalization uptake, cargos acting on cells. Especially, also explored role several aspects health, such as immunological homeostasis, barrier, injury, inflammatory bowel disease, necrotizing enterocolitis, malignancy, harm other organs connected intestine. We expected use or miRNAs new dietary additives functional regulatory factors. future, will gain more knowledge about substantial impact human intestines health-related matters.

Language: Английский

Citations

0
Influence of Maternal Diet and Lactation Time on the Exosomal miRNA Cargo in Breast Milk DOI Creative Commons

Laura Sanjulián,

Alexandre Lamas, Rocío Barreiro

et al.

Foods, Journal Year: 2025, Volume and Issue: 14(6), P. 1003 - 1003

Published: March 16, 2025

The importance of breastfeeding for maternal and infant health is widely accepted. In this sense, the presence macro micronutrients in human milk ensures proper safe nutrition, along with multiple bioactive molecules that modulate newborn's immunity. Usually packaged within exosomes, miRNAs circulating are bioavailable to breastfed infants. Their role development poorly understood, mainly because miRNA cargo has not been fully elucidated date. objective study assess eleven (miR-148a-3p, miR-29b-3p, miR-125b-5p, miR-200c-3p, let-7f-5p, let-7b-5p, let-7a-5p, miR-92a, miR-181a-3p, miR-30a-5p miR-155-5p) breast exosomes determine impact lactation time factors on their levels. Samples were collected from a cohort 59 Spanish mothers, ranging 1 months lactation. MiRNAs analyzed using reverse transcription qPCR. Lactation showed significant noteworthy downregulation miR-148a-3p, miR-200c-3p miR-125b-5p hsa-let-7a-5p. levels correlated some fatty acids such as arachidonic acid DHA. Also, positive correlation between Se was found, linked upregulated miR-125b. results work demonstrate dynamic, probably aim adapting specific needs infant.

Language: Английский

Citations

0
The Protective Role of Exosome-Derived MicroRNAs and Proteins from Human Breast Milk against Infectious Agents DOI Creative Commons

Ki-Uk Kim,

Kyusun Han,

Jisu Kim

et al.

Metabolites, Journal Year: 2023, Volume and Issue: 13(5), P. 635 - 635

Published: May 8, 2023

Human breast milk (HBM)-derived exosomes contain various biological and immunological components. However, comprehensive immune-related antimicrobial factor analysis requires transcriptomic, proteomic, multiple databases for functional analyses, has yet to be conducted. Therefore, we isolated confirmed HBM-derived by detecting specific markers examining their morphology using western blot transmission electron microscopy. Moreover, implemented small RNA sequencing liquid chromatography-mass spectrometry investigate substances within the roles in combating pathogenic effects, identifying 208 miRNAs 377 proteins associated with pathways diseases. Integrated omics analyses identified a connection between exosomal microbial infections. In addition, gene ontology Kyoto Encyclopedia of Genes Genomes pathway demonstrated that miRNA influence functions Finally, protein-protein interaction three primary (ICAM1, TLR2, FN1) infections mediating pro-inflammation, controlling infection, facilitating elimination. Our findings determine modulate immune system could offer therapeutic strategies regulating infection.

Language: Английский

Citations

10
Unraveling the Multifaceted Roles of Extracellular Vesicles: Insights into Biology, Pharmacology, and Pharmaceutical Applications for Drug Delivery DOI Open Access
Ali Al-Jipouri, Àuria Eritja, Milica Božić

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 25(1), P. 485 - 485

Published: Dec. 29, 2023

Extracellular vesicles (EVs) are nanoparticles released from various cell types that have emerged as powerful new therapeutic option for a variety of diseases. EVs involved in the transmission biological signals between cells and regulation processes, highlighting them potential novel targets/platforms therapeutics intervention and/or delivery. Therefore, it is necessary to investigate aspects EVs' biogenesis, biodistribution, metabolism, excretion well safety/compatibility both unmodified engineered upon administration different pharmaceutical dosage forms delivery systems. In this review, we summarize current knowledge essential physiological pathological roles organs organ We provide an overview regarding application targets, therapeutics, drug platforms. also explore approaches implemented over years improve specific EV products routes.

Language: Английский

Citations

10
Recent insights into breast milk microRNA: their role as functional regulators DOI Creative Commons
Yiran Xu, Jinglu Zhao, Hsi‐Yuan Huang

et al.

Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11

Published: April 16, 2024

Breast milk (BM) is a primary biofluid that plays crucial role in infant development and the regulation of immune system. As class rich biomolecules BM, microRNAs (miRNAs) are regarded as active factors contributing to growth development. Surprisingly, these molecules exhibit resilience harsh conditions, providing an opportunity for infants absorb them. In addition, many studies have shown miRNAs breast milk, when absorbed into gastrointestinal system, can act functional regulators effectively regulate gene expression. Understanding absorption pattern BM miRNA may facilitate creation formula with more optimal balance pave way novel drug delivery techniques. this review, we initially present evidence absorption. Subsequently, compile integrate both vivo vitro findings illustrate bioavailability biodistribution post-absorption. evaluate strengths weaknesses previous discuss potential variables discrepancies their outcomes. This literature review indicates be regulatory agents.

Language: Английский

Citations

3